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The map label for this gene is dut [H]

Identifier: 157963650

GI number: 157963650

Start: 4666089

End: 4666547

Strand: Direct

Name: dut [H]

Synonym: Spea_3839

Alternate gene names: 157963650

Gene position: 4666089-4666547 (Clockwise)

Preceding gene: 157963649

Following gene: 157963651

Centisome position: 90.17

GC content: 48.37

Gene sequence:

>459_bases
ATGAAAACACCAATCGAATTAAAGATTTTAGACTCACGCATCGGCACCGAGTTTCCACTTCCTGCTTACGCCACACCAGG
CAGTGCAGGCATGGATCTTCGCGCCATCACAGATACCCAACTTGTTATCCAGCCGGGTGAAACAGTGCTAATCCCTACGG
GAATTGCCATTCACGTTGCAGACCCTAGCCTAGCGGCAATTATTTTACCGCGCTCAGGATTAGGCCATAAGCACGGCATC
GTTCTAGGCAATCTAGTTGGACTTATCGATTCGGATTATCAAGGACCTCTAATGGTTTCTTGCTGGAACCGTGGCAGTGA
GCCTTTTACAATCGAAATCGGTGACAGACTCGCGCAATTGGTCTTTGTACCGGTTGTTCAGGCTGAATTTAAACTGGTAG
ACGAGTTTAATCAGTCAGATCGCGGCGCAGGTGGATTTGGCCACTCAGGAACCAAATAA

Upstream 100 bases:

>100_bases
GCGTATTTTGGAGTGATGGTAGCACCGACCTACCCGCTGTTGATAAAGACACTTTAGCCAAGCAATTGCTTACTCTAATA
GCGAACAAAATAAAAAACTG

Downstream 100 bases:

>100_bases
CTGATTGTTTAGCTTTTTACGCAATCACTTTTAATGGATAGCATGGAGCAATGTTTCCTTGCCGTCTCGCTCAGTAGTAA
TCGAGTGAAGGACAGATAAA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase [H]

Number of amino acids: Translated: 152; Mature: 152

Protein sequence:

>152_residues
MKTPIELKILDSRIGTEFPLPAYATPGSAGMDLRAITDTQLVIQPGETVLIPTGIAIHVADPSLAAIILPRSGLGHKHGI
VLGNLVGLIDSDYQGPLMVSCWNRGSEPFTIEIGDRLAQLVFVPVVQAEFKLVDEFNQSDRGAGGFGHSGTK

Sequences:

>Translated_152_residues
MKTPIELKILDSRIGTEFPLPAYATPGSAGMDLRAITDTQLVIQPGETVLIPTGIAIHVADPSLAAIILPRSGLGHKHGI
VLGNLVGLIDSDYQGPLMVSCWNRGSEPFTIEIGDRLAQLVFVPVVQAEFKLVDEFNQSDRGAGGFGHSGTK
>Mature_152_residues
MKTPIELKILDSRIGTEFPLPAYATPGSAGMDLRAITDTQLVIQPGETVLIPTGIAIHVADPSLAAIILPRSGLGHKHGI
VLGNLVGLIDSDYQGPLMVSCWNRGSEPFTIEIGDRLAQLVFVPVVQAEFKLVDEFNQSDRGAGGFGHSGTK

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA [H]

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family [H]

Homologues:

Organism=Homo sapiens, GI70906444, Length=145, Percent_Identity=34.4827586206897, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI4503423, Length=145, Percent_Identity=34.4827586206897, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI70906441, Length=145, Percent_Identity=34.4827586206897, Blast_Score=77, Evalue=8e-15,
Organism=Escherichia coli, GI1790071, Length=148, Percent_Identity=69.5945945945946, Blast_Score=225, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71988561, Length=154, Percent_Identity=33.7662337662338, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319729, Length=84, Percent_Identity=45.2380952380952, Blast_Score=72, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24583610, Length=132, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921126, Length=132, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008180
- InterPro:   IPR008181 [H]

Pfam domain/function: PF00692 dUTPase [H]

EC number: =3.6.1.23 [H]

Molecular weight: Translated: 16144; Mature: 16144

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTPIELKILDSRIGTEFPLPAYATPGSAGMDLRAITDTQLVIQPGETVLIPTGIAIHVA
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCEEEEECCEEEEEC
DPSLAAIILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRGSEPFTIEIGDRLAQL
CCCEEEEEEECCCCCCCCCEEEEHEEEEECCCCCCCEEEEEECCCCCCEEEEHHHHHHHH
VFVPVVQAEFKLVDEFNQSDRGAGGFGHSGTK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKTPIELKILDSRIGTEFPLPAYATPGSAGMDLRAITDTQLVIQPGETVLIPTGIAIHVA
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCEEEEECCCEEEEECCCEEEEECCEEEEEC
DPSLAAIILPRSGLGHKHGIVLGNLVGLIDSDYQGPLMVSCWNRGSEPFTIEIGDRLAQL
CCCEEEEEEECCCCCCCCCEEEEHEEEEECCCCCCCEEEEEECCCCCCEEEEHHHHHHHH
VFVPVVQAEFKLVDEFNQSDRGAGGFGHSGTK
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA