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The map label for this gene is leuA [H]

Identifier: 157963639

GI number: 157963639

Start: 4653521

End: 4655092

Strand: Reverse

Name: leuA [H]

Synonym: Spea_3828

Alternate gene names: 157963639

Gene position: 4655092-4653521 (Counterclockwise)

Preceding gene: 157963641

Following gene: 157963638

Centisome position: 89.96

GC content: 47.96

Gene sequence:

>1572_bases
ATGTCTAACCGAGTGATTATATTTGACACCACCCTACGCGATGGTGAACAAGCATTAGCAGCAAGTTTAACGGTTAAGGA
AAAACTTCAAATTGCACTCGCGTTAGAGCGTTTAGGTGTCGATGTGATGGAAGTTGGCTTTCCCGTTTCTTCTCCTGGTG
ACTTCGAGTCGGTACAAACCATTGCTAAAACTATTAAAAATAGCCGAGTTTGTGCCCTATCACGTGCATTAGAAAAGGAT
ATCGATGCCGCTGCACAGGCCTTGTCAGTGGCCGAGCAGTTCCGTATTCATACCTTTATTTCAACTTCGACCATTCACGT
CGAGAGTAAATTAAAGCGCTCATTTGATCAGGTGCTGGAAATGGCGGTTGGCGCTGTTAAGTATGCCCGCCGTTTCACCG
ATGATGTGGAGTTTTCTTGTGAAGACGCAGGCCGTACACCTATCGATAACTTATGTCGCATGGTGGAGGAGGCGATTAAA
GCTGGCGCGCGTACCATTAATATTCCCGACACTGTGGGTTATACGGTACCGAGTGAGTTTGGCGGCATCATTCAGACTCT
GTTTAATCGGGTACCCAATATCGACCAAGCGGTGATCTCGGTGCATTGTCATGACGATTTAGGTTTGTCGGTTGCTAACT
CTATTACTGCCGTGCAGCACGGAGCTCGCCAAATAGAGTGTACCGTCAACGGTATTGGTGAGCGTGCGGGTAATTGTTCT
CTAGAAGAGATCGCCATGATCTTATCGACTCGCAAGGGTGAGCTAGGACTTGAGACGGGTATTAATGCCAAAGAGATCCA
CCGTACCTCAAGCTTGGTTAGTCAACTTTGTAATATGCCTGTGCAGGCGAATAAGGCCATTGTTGGCGCGAATGCATTCA
CTCACTCATCGGGCATTCATCAAGATGGCATGCTAAAAGCGCAAAACACCTATGAGATTATGACGCCTGAGAGCATTGGC
TTAAATCGCAATAACTTGAATATGACCTCACGCTCTGGCCGTCATGTGATTAAGCACCGTATGAGTGAGCTGGGCTATGG
TGAGCAAGATTATAATATGGATGTCTTGTATGAGGAGTTTTTGACCCTAGCAGATAAGAAGGGTCAGGTGTTTGATTATG
ACCTAGAGGCGCTTGCCTTTATGGAAGCTCAAGCCGAAACCGATGATCACTATAAATTACAGCAGCTAGTAGTACATTCA
GACTCTACAGAAGGCAGCGCAACAGCTACGGTCAAGGTTGCGGTTAACGGTGAAACCATTACCGAAGCCGCGACGGGTAA
TGGTCCTGTCGATGCCGCTTATAAGGCCGTTGCTCGGGCCAGTGGTTGTGAAATCAACATTAGCAGTTACCAGTTAAGCG
CAAAAGGCGAGGGGCAAAATGCCTTGGGCCAAGTGGATATTACCGCTAAGTATCGCGAGCAAAACTTCCATGGTGTTGGC
TTAGCTACCGACGTGGTTGAGGCGTCAGCCAAGGCTTTGGTCCATGTGATGAACCTAACTTGGCGCGCCGATAAAGTCGC
CGATTGTAAGCAGCGTATTCAGCAGAACAAGCGAGAGCTCGGTGGCGTGTAA

Upstream 100 bases:

>100_bases
CATTAATAAACCCCGCAAATAGAGCGGGGTTTTTTGTATTTTAATCGTAACAAGCAAAGTGAACACATAACGGAACAGTG
ATGTTCAATGGAGAAACTAG

Downstream 100 bases:

>100_bases
GCCGTAGAGTCGATACTAATGTGTTGAGCGCAAGAATATGTCTAAAGAATAAATTGAAGAATAAACCACAGGAGTGAGCC
GAAGTATGAGTTATCAGATT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MSNRVIIFDTTLRDGEQALAASLTVKEKLQIALALERLGVDVMEVGFPVSSPGDFESVQTIAKTIKNSRVCALSRALEKD
IDAAAQALSVAEQFRIHTFISTSTIHVESKLKRSFDQVLEMAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEEAIK
AGARTINIPDTVGYTVPSEFGGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTVNGIGERAGNCS
LEEIAMILSTRKGELGLETGINAKEIHRTSSLVSQLCNMPVQANKAIVGANAFTHSSGIHQDGMLKAQNTYEIMTPESIG
LNRNNLNMTSRSGRHVIKHRMSELGYGEQDYNMDVLYEEFLTLADKKGQVFDYDLEALAFMEAQAETDDHYKLQQLVVHS
DSTEGSATATVKVAVNGETITEAATGNGPVDAAYKAVARASGCEINISSYQLSAKGEGQNALGQVDITAKYREQNFHGVG
LATDVVEASAKALVHVMNLTWRADKVADCKQRIQQNKRELGGV

Sequences:

>Translated_523_residues
MSNRVIIFDTTLRDGEQALAASLTVKEKLQIALALERLGVDVMEVGFPVSSPGDFESVQTIAKTIKNSRVCALSRALEKD
IDAAAQALSVAEQFRIHTFISTSTIHVESKLKRSFDQVLEMAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEEAIK
AGARTINIPDTVGYTVPSEFGGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTVNGIGERAGNCS
LEEIAMILSTRKGELGLETGINAKEIHRTSSLVSQLCNMPVQANKAIVGANAFTHSSGIHQDGMLKAQNTYEIMTPESIG
LNRNNLNMTSRSGRHVIKHRMSELGYGEQDYNMDVLYEEFLTLADKKGQVFDYDLEALAFMEAQAETDDHYKLQQLVVHS
DSTEGSATATVKVAVNGETITEAATGNGPVDAAYKAVARASGCEINISSYQLSAKGEGQNALGQVDITAKYREQNFHGVG
LATDVVEASAKALVHVMNLTWRADKVADCKQRIQQNKRELGGV
>Mature_522_residues
SNRVIIFDTTLRDGEQALAASLTVKEKLQIALALERLGVDVMEVGFPVSSPGDFESVQTIAKTIKNSRVCALSRALEKDI
DAAAQALSVAEQFRIHTFISTSTIHVESKLKRSFDQVLEMAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEEAIKA
GARTINIPDTVGYTVPSEFGGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTVNGIGERAGNCSL
EEIAMILSTRKGELGLETGINAKEIHRTSSLVSQLCNMPVQANKAIVGANAFTHSSGIHQDGMLKAQNTYEIMTPESIGL
NRNNLNMTSRSGRHVIKHRMSELGYGEQDYNMDVLYEEFLTLADKKGQVFDYDLEALAFMEAQAETDDHYKLQQLVVHSD
STEGSATATVKVAVNGETITEAATGNGPVDAAYKAVARASGCEINISSYQLSAKGEGQNALGQVDITAKYREQNFHGVGL
ATDVVEASAKALVHVMNLTWRADKVADCKQRIQQNKRELGGV

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=516, Percent_Identity=66.2790697674419, Blast_Score=706, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324225, Length=535, Percent_Identity=25.607476635514, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6320019, Length=350, Percent_Identity=28.2857142857143, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320071, Length=346, Percent_Identity=28.3236994219653, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6324682, Length=544, Percent_Identity=24.6323529411765, Blast_Score=124, Evalue=5e-29,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005671
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 56786; Mature: 56655

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRVIIFDTTLRDGEQALAASLTVKEKLQIALALERLGVDVMEVGFPVSSPGDFESVQT
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IAKTIKNSRVCALSRALEKDIDAAAQALSVAEQFRIHTFISTSTIHVESKLKRSFDQVLE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEHHHHHHHHHHHHHH
MAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEEAIKAGARTINIPDTVGYTVPSEF
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHH
GGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTVNGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEIAMILSTRKGELGLETGINAKEIHRTSSLVSQLCNMPVQANKAIVGANAFTHSSGIH
HHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEECCCCCC
QDGMLKAQNTYEIMTPESIGLNRNNLNMTSRSGRHVIKHRMSELGYGEQDYNMDVLYEEF
CCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LTLADKKGQVFDYDLEALAFMEAQAETDDHYKLQQLVVHSDSTEGSATATVKVAVNGETI
HHHHCCCCCEEECCHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCCEEEEEEEEECCCEE
TEAATGNGPVDAAYKAVARASGCEINISSYQLSAKGEGQNALGQVDITAKYREQNFHGVG
EECCCCCCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCC
LATDVVEASAKALVHVMNLTWRADKVADCKQRIQQNKRELGGV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNRVIIFDTTLRDGEQALAASLTVKEKLQIALALERLGVDVMEVGFPVSSPGDFESVQT
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCHHHHHH
IAKTIKNSRVCALSRALEKDIDAAAQALSVAEQFRIHTFISTSTIHVESKLKRSFDQVLE
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEHHHHHHHHHHHHHH
MAVGAVKYARRFTDDVEFSCEDAGRTPIDNLCRMVEEAIKAGARTINIPDTVGYTVPSEF
HHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCCHHH
GGIIQTLFNRVPNIDQAVISVHCHDDLGLSVANSITAVQHGARQIECTVNGIGERAGNCS
HHHHHHHHHHCCCCCEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCC
LEEIAMILSTRKGELGLETGINAKEIHRTSSLVSQLCNMPVQANKAIVGANAFTHSSGIH
HHHHHHHHHCCCCCCCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEECCCCCC
QDGMLKAQNTYEIMTPESIGLNRNNLNMTSRSGRHVIKHRMSELGYGEQDYNMDVLYEEF
CCCCEEECCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
LTLADKKGQVFDYDLEALAFMEAQAETDDHYKLQQLVVHSDSTEGSATATVKVAVNGETI
HHHHCCCCCEEECCHHHHHHHHHHCCCCCHHHHHHHHEECCCCCCCEEEEEEEEECCCEE
TEAATGNGPVDAAYKAVARASGCEINISSYQLSAKGEGQNALGQVDITAKYREQNFHGVG
EECCCCCCCHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCEEEEEEEECCCCCCCCCC
LATDVVEASAKALVHVMNLTWRADKVADCKQRIQQNKRELGGV
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA