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The map label for this gene is leuB [H]

Identifier: 157963638

GI number: 157963638

Start: 4652341

End: 4653435

Strand: Reverse

Name: leuB [H]

Synonym: Spea_3827

Alternate gene names: 157963638

Gene position: 4653435-4652341 (Counterclockwise)

Preceding gene: 157963639

Following gene: 157963637

Centisome position: 89.93

GC content: 49.13

Gene sequence:

>1095_bases
ATGAGTTATCAGATTGCAGTACTGGCCGGAGATGGCATTGGACCAGAGGTGATGGCTGAAGCACGTAAGGTGTTAAAAGC
AGTTGAACAACGTTTTACACTTTCTATTGAATATTCTGAGTACGATGTTGGCGGCGCCGCTATCGATAATCATGGCTGCC
CACTTCCAGAGCAAACCCTAAAAGGCTGTGAAGCTGCAGATGCGATTTTGTTTGGCTCGGTAGGCGGTCCTAAATGGGAA
CACTTAGCCCCAAACGATCAACCTGAGCGTGGTGCATTATTGCCACTTCGCGGCCATTTTGAGTTGTTCTGTAATATGCG
CCCTGCCAAATTACACGCAGGTCTTGAGCATATGTCACCATTGCGCAGCGATATCTCTTCTCGTGGTTTTGATGTGGTTT
GTGTTCGCGAGCTAACAAGCGGGATCTACTTTGGTAAGCCCAAGGGGCGCCAAGGTGAAGGCGAAAATGAAGAAGCCTTC
GATACTATGCGTTATAGCCGTAAAGAGATTAGACGCATTGCAAAAATCGCCTTCGAGGCAGCTCAAGGTCGTCGTAAGAA
AGTCACTTCGGTAGACAAAGCCAACGTGCTCGCCTGTTCGGTGTTATGGCGTGAAGTGGTTGAAGAGGTTGCTAAAGAGT
TTCCAGAGGTCGAGCTAGAGCATATCTATATCGATAACGCGACTATGCAGCTACTACGCCGCCCCGATGAGTTCGATGTC
ATGCTGTGTTCAAACCTTTTCGGCGACATCATCTCTGATGAGATAGCTATGCTAACAGGCTCAATGGGATTACTGTCTTC
AATTAGCATGAACAGCTCTGGTTTTGGTTTGTATGAGCCTGCTGGTGGCAGTGCGCCAGATATCGCGGGTCAGGGCATTG
CTAACCCTGTGGCGCAAATTCTATCGGCAGCCTTGTTGTTGCGTCATAGCCTTAAACTTGAGGACGCGGCAACGGCCATT
GAAAACGCCGTAGGAAAAGCGCTGAGTGATGGCTACTTGACGGGGGAATTGTTGCCTGCGGAGCAGCGTCACAATGCAAA
ATCGACTAGCGAGATGGGAGATTACATCTCTCAGTTAGTAAGAGAGGGGGTGTAA

Upstream 100 bases:

>100_bases
CTCGGTGGCGTGTAAGCCGTAGAGTCGATACTAATGTGTTGAGCGCAAGAATATGTCTAAAGAATAAATTGAAGAATAAA
CCACAGGAGTGAGCCGAAGT

Downstream 100 bases:

>100_bases
TCATGGCTAAGACACTATATGAGAAAGTATGGGACAGCCATGTGGTTGTCGCCCCTGAAGGCGAAGCGCCCATTATCTAT
GTTGATAGGCATCTAGTGCA

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSYQIAVLAGDGIGPEVMAEARKVLKAVEQRFTLSIEYSEYDVGGAAIDNHGCPLPEQTLKGCEAADAILFGSVGGPKWE
HLAPNDQPERGALLPLRGHFELFCNMRPAKLHAGLEHMSPLRSDISSRGFDVVCVRELTSGIYFGKPKGRQGEGENEEAF
DTMRYSRKEIRRIAKIAFEAAQGRRKKVTSVDKANVLACSVLWREVVEEVAKEFPEVELEHIYIDNATMQLLRRPDEFDV
MLCSNLFGDIISDEIAMLTGSMGLLSSISMNSSGFGLYEPAGGSAPDIAGQGIANPVAQILSAALLLRHSLKLEDAATAI
ENAVGKALSDGYLTGELLPAEQRHNAKSTSEMGDYISQLVREGV

Sequences:

>Translated_364_residues
MSYQIAVLAGDGIGPEVMAEARKVLKAVEQRFTLSIEYSEYDVGGAAIDNHGCPLPEQTLKGCEAADAILFGSVGGPKWE
HLAPNDQPERGALLPLRGHFELFCNMRPAKLHAGLEHMSPLRSDISSRGFDVVCVRELTSGIYFGKPKGRQGEGENEEAF
DTMRYSRKEIRRIAKIAFEAAQGRRKKVTSVDKANVLACSVLWREVVEEVAKEFPEVELEHIYIDNATMQLLRRPDEFDV
MLCSNLFGDIISDEIAMLTGSMGLLSSISMNSSGFGLYEPAGGSAPDIAGQGIANPVAQILSAALLLRHSLKLEDAATAI
ENAVGKALSDGYLTGELLPAEQRHNAKSTSEMGDYISQLVREGV
>Mature_363_residues
SYQIAVLAGDGIGPEVMAEARKVLKAVEQRFTLSIEYSEYDVGGAAIDNHGCPLPEQTLKGCEAADAILFGSVGGPKWEH
LAPNDQPERGALLPLRGHFELFCNMRPAKLHAGLEHMSPLRSDISSRGFDVVCVRELTSGIYFGKPKGRQGEGENEEAFD
TMRYSRKEIRRIAKIAFEAAQGRRKKVTSVDKANVLACSVLWREVVEEVAKEFPEVELEHIYIDNATMQLLRRPDEFDVM
LCSNLFGDIISDEIAMLTGSMGLLSSISMNSSGFGLYEPAGGSAPDIAGQGIANPVAQILSAALLLRHSLKLEDAATAIE
NAVGKALSDGYLTGELLPAEQRHNAKSTSEMGDYISQLVREGV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=343, Percent_Identity=32.3615160349854, Blast_Score=137, Evalue=1e-32,
Organism=Homo sapiens, GI28178821, Length=334, Percent_Identity=32.0359281437126, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI28178816, Length=334, Percent_Identity=32.0359281437126, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI28178838, Length=338, Percent_Identity=29.585798816568, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI4758582, Length=329, Percent_Identity=30.3951367781155, Blast_Score=122, Evalue=4e-28,
Organism=Homo sapiens, GI28178819, Length=162, Percent_Identity=39.5061728395062, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=67.7685950413223, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI1788101, Length=366, Percent_Identity=34.9726775956284, Blast_Score=182, Evalue=4e-47,
Organism=Escherichia coli, GI1787381, Length=402, Percent_Identity=25.1243781094527, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=342, Percent_Identity=31.8713450292398, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17550882, Length=341, Percent_Identity=29.9120234604106, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI17505779, Length=330, Percent_Identity=29.3939393939394, Blast_Score=121, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI25144293, Length=339, Percent_Identity=30.6784660766962, Blast_Score=119, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6319830, Length=370, Percent_Identity=43.2432432432432, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6322097, Length=347, Percent_Identity=33.7175792507205, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6324709, Length=339, Percent_Identity=30.6784660766962, Blast_Score=129, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6324291, Length=346, Percent_Identity=28.9017341040462, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24643268, Length=342, Percent_Identity=34.7953216374269, Blast_Score=167, Evalue=9e-42,
Organism=Drosophila melanogaster, GI24643270, Length=342, Percent_Identity=34.7953216374269, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24661184, Length=334, Percent_Identity=30.5389221556886, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI161078637, Length=335, Percent_Identity=29.8507462686567, Blast_Score=115, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161078633, Length=335, Percent_Identity=29.8507462686567, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI24650122, Length=335, Percent_Identity=29.8507462686567, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161078635, Length=333, Percent_Identity=30.03003003003, Blast_Score=114, Evalue=7e-26,
Organism=Drosophila melanogaster, GI161078639, Length=329, Percent_Identity=30.3951367781155, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI281362242, Length=333, Percent_Identity=27.3273273273273, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24648872, Length=333, Percent_Identity=27.3273273273273, Blast_Score=112, Evalue=4e-25,
Organism=Drosophila melanogaster, GI20130355, Length=338, Percent_Identity=25.4437869822485, Blast_Score=85, Evalue=7e-17,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39542; Mature: 39411

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQIAVLAGDGIGPEVMAEARKVLKAVEQRFTLSIEYSEYDVGGAAIDNHGCPLPEQTL
CCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEECCCCCCCCHHHH
KGCEAADAILFGSVGGPKWEHLAPNDQPERGALLPLRGHFELFCNMRPAKLHAGLEHMSP
CCCHHHHHEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHCHHHHHH
LRSDISSRGFDVVCVRELTSGIYFGKPKGRQGEGENEEAFDTMRYSRKEIRRIAKIAFEA
HHHHHHHCCCCEEEHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQGRRKKVTSVDKANVLACSVLWREVVEEVAKEFPEVELEHIYIDNATMQLLRRPDEFDV
HCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCHHHH
MLCSNLFGDIISDEIAMLTGSMGLLSSISMNSSGFGLYEPAGGSAPDIAGQGIANPVAQI
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LSAALLLRHSLKLEDAATAIENAVGKALSDGYLTGELLPAEQRHNAKSTSEMGDYISQLV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHH
REGV
HCCC
>Mature Secondary Structure 
SYQIAVLAGDGIGPEVMAEARKVLKAVEQRFTLSIEYSEYDVGGAAIDNHGCPLPEQTL
CEEEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCEECCCCCCCCHHHH
KGCEAADAILFGSVGGPKWEHLAPNDQPERGALLPLRGHFELFCNMRPAKLHAGLEHMSP
CCCHHHHHEEEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEECCCHHHHHHCHHHHHH
LRSDISSRGFDVVCVRELTSGIYFGKPKGRQGEGENEEAFDTMRYSRKEIRRIAKIAFEA
HHHHHHHCCCCEEEHHHHHCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
AQGRRKKVTSVDKANVLACSVLWREVVEEVAKEFPEVELEHIYIDNATMQLLRRPDEFDV
HCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECHHHHHHHHCCCHHHH
MLCSNLFGDIISDEIAMLTGSMGLLSSISMNSSGFGLYEPAGGSAPDIAGQGIANPVAQI
HHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHH
LSAALLLRHSLKLEDAATAIENAVGKALSDGYLTGELLPAEQRHNAKSTSEMGDYISQLV
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHH
REGV
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]