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The map label for this gene is leuC [H]

Identifier: 157963637

GI number: 157963637

Start: 4650938

End: 4652338

Strand: Reverse

Name: leuC [H]

Synonym: Spea_3826

Alternate gene names: 157963637

Gene position: 4652338-4650938 (Counterclockwise)

Preceding gene: 157963638

Following gene: 157963636

Centisome position: 89.91

GC content: 50.61

Gene sequence:

>1401_bases
ATGGCTAAGACACTATATGAGAAAGTATGGGACAGCCATGTGGTTGTCGCCCCTGAAGGCGAAGCGCCCATTATCTATGT
TGATAGGCATCTAGTGCATGAGGTGACCTCACCCCAAGCATTTAGTGGAGTTAAGGTCGCTGGCCGACAGTTACGTGCGC
CACAGAAAACCTTCGCCACCATGGATCACAACACATCCACAACCAGCGCAAGCTTAGATGCACTTAGCCCTATGGCGCGT
ACTCAGGTTGAAACTCTAGAGCAAAACTGTAAAGAGTTCGGCGTTCGCCTCTATGATATTCACCATAAGAATCAAGGTAT
TGTGCATGTGATGGGCCCAGAGCTTGGCATTACTTTGCCGGGAACTGTGATTGTGTGTGGTGACTCGCATACCGCCACCC
ATGGCGCCTTTGGTGCTCTTGCATTTGGTATCGGTACATCGGAAGTTGAGCATGTGATGGCGACGCAGACACTACGTCAG
CTAAAGGCCAAGACCATGAAGATTGAAGTTCGTGGTCATGTGGCTTCTGGGATCACCGCTAAAGATATCGTGTTAGCGAT
TATTGGTAAAATTGGCATGGATGGCGGCACAGGTTATGTGGTTGAGTTCTGTGGTGAAGCAATCGAGGCGCTGAGCATGG
AAGGCCGCATGACAGTATGCAATATGGCCATCGAGATGGGCGCAAAGGCAGGCATGATAGCCCCAGATGCGACGACGGCT
GAATACCTTGAAGGTCGTGAGTTTTCACCAAAAGGTGAAAGCTGGAGCCAAGCCGTTGCTGCTTGGGAGCAGTTAAAAAC
CGACGAAGGTGCAGTATTTGATGCCAGTGTGGTGTTAGATGCCATAGATATTGCACCGCAACTGACTTGGGGAACCAACC
CAGGCCAGGTGGTTGCTATCGATCAGTTAGTGCCAAACCCACTCGATGCCACCAACTCTACCGTTCGTAGTAGTATCGAG
AAGGCGCTGGAGTATGTCGATTTAACAGCGGGCACCTTGATGACAGATGTCAGTATCAATAAAGTATTTATTGGTTCTTG
TACTAACTCTCGCATTGAGGATCTTCGCGCCGCAGCGGTTCACGCCAAGGGGCGCAAGGTCGCTGATGGGGTAAAGGCTA
TTGTTGTACCAGGCTCAGGGCTAGTTAAAGAGCAAGCCGAGGCTGAAGGTTTAGATAAGATCTTTACTGATGCCGGTTTT
GAATGGCGCTTACCGGGTTGCTCTATGTGTTTAGCTATGAATGACGATAAATTGGAAGCGGGCGATCGCTGCGCGTCGAC
TAGTAATCGTAATTTTGAGGGCCGACAAGGGCGTGGCAGCCGTACTCACTTAGTGAGCCCTGCTATGGCCGCCGCTGCTG
CGGTAGCCGGACACTTTGTTGATATTCGCAAACCATACTAG

Upstream 100 bases:

>100_bases
TGACGGGGGAATTGTTGCCTGCGGAGCAGCGTCACAATGCAAAATCGACTAGCGAGATGGGAGATTACATCTCTCAGTTA
GTAAGAGAGGGGGTGTAATC

Downstream 100 bases:

>100_bases
GAGCATAGCATGCAACCATTTATCTCACATACAGGGCTTGCGGTTATCATAGATAGTGCAAACGTGGATACCGATCAGAT
CATCCCTAAGCAGTTTCTTT

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MAKTLYEKVWDSHVVVAPEGEAPIIYVDRHLVHEVTSPQAFSGVKVAGRQLRAPQKTFATMDHNTSTTSASLDALSPMAR
TQVETLEQNCKEFGVRLYDIHHKNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLRQ
LKAKTMKIEVRGHVASGITAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMIAPDATTA
EYLEGREFSPKGESWSQAVAAWEQLKTDEGAVFDASVVLDAIDIAPQLTWGTNPGQVVAIDQLVPNPLDATNSTVRSSIE
KALEYVDLTAGTLMTDVSINKVFIGSCTNSRIEDLRAAAVHAKGRKVADGVKAIVVPGSGLVKEQAEAEGLDKIFTDAGF
EWRLPGCSMCLAMNDDKLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY

Sequences:

>Translated_466_residues
MAKTLYEKVWDSHVVVAPEGEAPIIYVDRHLVHEVTSPQAFSGVKVAGRQLRAPQKTFATMDHNTSTTSASLDALSPMAR
TQVETLEQNCKEFGVRLYDIHHKNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLRQ
LKAKTMKIEVRGHVASGITAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMIAPDATTA
EYLEGREFSPKGESWSQAVAAWEQLKTDEGAVFDASVVLDAIDIAPQLTWGTNPGQVVAIDQLVPNPLDATNSTVRSSIE
KALEYVDLTAGTLMTDVSINKVFIGSCTNSRIEDLRAAAVHAKGRKVADGVKAIVVPGSGLVKEQAEAEGLDKIFTDAGF
EWRLPGCSMCLAMNDDKLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
>Mature_465_residues
AKTLYEKVWDSHVVVAPEGEAPIIYVDRHLVHEVTSPQAFSGVKVAGRQLRAPQKTFATMDHNTSTTSASLDALSPMART
QVETLEQNCKEFGVRLYDIHHKNQGIVHVMGPELGITLPGTVIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLRQL
KAKTMKIEVRGHVASGITAKDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMIAPDATTAE
YLEGREFSPKGESWSQAVAAWEQLKTDEGAVFDASVVLDAIDIAPQLTWGTNPGQVVAIDQLVPNPLDATNSTVRSSIEK
ALEYVDLTAGTLMTDVSINKVFIGSCTNSRIEDLRAAAVHAKGRKVADGVKAIVVPGSGLVKEQAEAEGLDKIFTDAGFE
WRLPGCSMCLAMNDDKLEAGDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=363, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI41352693, Length=364, Percent_Identity=28.021978021978, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI8659555, Length=396, Percent_Identity=27.5252525252525, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786259, Length=464, Percent_Identity=70.9051724137931, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=367, Percent_Identity=30.2452316076294, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI87081781, Length=353, Percent_Identity=26.628895184136, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25149337, Length=365, Percent_Identity=29.8630136986301, Blast_Score=121, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=365, Percent_Identity=29.8630136986301, Blast_Score=121, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17568399, Length=447, Percent_Identity=27.5167785234899, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI25149342, Length=305, Percent_Identity=29.1803278688525, Blast_Score=111, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6321429, Length=469, Percent_Identity=60.3411513859275, Blast_Score=563, Evalue=1e-161,
Organism=Saccharomyces cerevisiae, GI6320440, Length=465, Percent_Identity=25.8064516129032, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=368, Percent_Identity=28.5326086956522, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6322261, Length=398, Percent_Identity=26.8844221105528, Blast_Score=121, Evalue=3e-28,
Organism=Drosophila melanogaster, GI281365315, Length=388, Percent_Identity=25.2577319587629, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI17864292, Length=388, Percent_Identity=25.2577319587629, Blast_Score=105, Evalue=8e-23,
Organism=Drosophila melanogaster, GI161076999, Length=388, Percent_Identity=25.2577319587629, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI28571643, Length=363, Percent_Identity=28.099173553719, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24645686, Length=456, Percent_Identity=26.5350877192982, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17137564, Length=440, Percent_Identity=27.0454545454545, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 49636; Mature: 49505

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTLYEKVWDSHVVVAPEGEAPIIYVDRHLVHEVTSPQAFSGVKVAGRQLRAPQKTFAT
CCHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCCHHCCHHHHHHHHCCCHHHHHC
MDHNTSTTSASLDALSPMARTQVETLEQNCKEFGVRLYDIHHKNQGIVHVMGPELGITLP
CCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCEECC
GTVIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLRQLKAKTMKIEVRGHVASGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCH
KDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMIAPDATTA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHH
EYLEGREFSPKGESWSQAVAAWEQLKTDEGAVFDASVVLDAIDIAPQLTWGTNPGQVVAI
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCEECCCCCCCEEEE
DQLVPNPLDATNSTVRSSIEKALEYVDLTAGTLMTDVSINKVFIGSCTNSRIEDLRAAAV
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECEECEEEEECCCCHHHHHHHHHHH
HAKGRKVADGVKAIVVPGSGLVKEQAEAEGLDKIFTDAGFEWRLPGCSMCLAMNDDKLEA
HCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCC
GDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
CCHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEECCCC
>Mature Secondary Structure 
AKTLYEKVWDSHVVVAPEGEAPIIYVDRHLVHEVTSPQAFSGVKVAGRQLRAPQKTFAT
CHHHHHHHCCCEEEEECCCCCCEEEECHHHHHHCCCCCHHCCHHHHHHHHCCCHHHHHC
MDHNTSTTSASLDALSPMARTQVETLEQNCKEFGVRLYDIHHKNQGIVHVMGPELGITLP
CCCCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCEECC
GTVIVCGDSHTATHGAFGALAFGIGTSEVEHVMATQTLRQLKAKTMKIEVRGHVASGITA
CEEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCH
KDIVLAIIGKIGMDGGTGYVVEFCGEAIEALSMEGRMTVCNMAIEMGAKAGMIAPDATTA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCHH
EYLEGREFSPKGESWSQAVAAWEQLKTDEGAVFDASVVLDAIDIAPQLTWGTNPGQVVAI
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHCCCCEECCCCCCCEEEE
DQLVPNPLDATNSTVRSSIEKALEYVDLTAGTLMTDVSINKVFIGSCTNSRIEDLRAAAV
HHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEECEECEEEEECCCCHHHHHHHHHHH
HAKGRKVADGVKAIVVPGSGLVKEQAEAEGLDKIFTDAGFEWRLPGCSMCLAMNDDKLEA
HCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCC
GDRCASTSNRNFEGRQGRGSRTHLVSPAMAAAAAVAGHFVDIRKPY
CCHHCCCCCCCCCCCCCCCCCCEECCHHHHHHHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA