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The map label for this gene is leuD [H]

Identifier: 157963636

GI number: 157963636

Start: 4650323

End: 4650928

Strand: Reverse

Name: leuD [H]

Synonym: Spea_3825

Alternate gene names: 157963636

Gene position: 4650928-4650323 (Counterclockwise)

Preceding gene: 157963637

Following gene: 157963635

Centisome position: 89.88

GC content: 44.55

Gene sequence:

>606_bases
ATGCAACCATTTATCTCACATACAGGGCTTGCGGTTATCATAGATAGTGCAAACGTGGATACCGATCAGATCATCCCTAA
GCAGTTTCTTTCTAAGGTGACGCGTGACGGCTTTGGTGTTCACCTTTTTCACGATTGGCGCTATTTAGATGACGCTGGCG
ATCAACTTAATCCAGACTTTACTCTGAATAAGCCTCGCTATCAGGGCGCTTCTATTCTTGTGTCTCAAGAAAACTTTGGC
TGTGGTTCGAGTCGAGAGCATGCGCCATGGGCGTTGGCCGATTTTGGCTTAAAAGTGATTATTGCCCCGAGCTTTGCCGA
TATCTTTTATGGTAACTCAATTAATAATGGCTTATTGCCTGTTAGGTTAGCTGACGCCGAAGTTAAACAGCTTATGGCTG
AGGTTGAGGCTAAAGAGGGAGCGGAGATCAGTGTTGATCTTCAAGCTTTAACTGTGACGAGCCCGTCAGGCTCTCAATTC
CATTTTGAAATTGCCCAATCGGCAAGGCATAACTTATTAAATGGGTTAGATGCAATTGGTCTGACACTGTCACATGCCGA
TACGATTGCTAATTATGAAGCTAACATTCCATCTTGGAGACGCTAA

Upstream 100 bases:

>100_bases
GCGTGGCAGCCGTACTCACTTAGTGAGCCCTGCTATGGCCGCCGCTGCTGCGGTAGCCGGACACTTTGTTGATATTCGCA
AACCATACTAGGAGCATAGC

Downstream 100 bases:

>100_bases
TAACATTAAAGTCGTGTCTAAAAGTTAGCGTACATATGTACCATTATTGAGTTTTGATGAAGCCATTTAGATTTTTTCTA
AATGGCTTTGTTTATTTTAG

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MQPFISHTGLAVIIDSANVDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDQLNPDFTLNKPRYQGASILVSQENFG
CGSSREHAPWALADFGLKVIIAPSFADIFYGNSINNGLLPVRLADAEVKQLMAEVEAKEGAEISVDLQALTVTSPSGSQF
HFEIAQSARHNLLNGLDAIGLTLSHADTIANYEANIPSWRR

Sequences:

>Translated_201_residues
MQPFISHTGLAVIIDSANVDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDQLNPDFTLNKPRYQGASILVSQENFG
CGSSREHAPWALADFGLKVIIAPSFADIFYGNSINNGLLPVRLADAEVKQLMAEVEAKEGAEISVDLQALTVTSPSGSQF
HFEIAQSARHNLLNGLDAIGLTLSHADTIANYEANIPSWRR
>Mature_201_residues
MQPFISHTGLAVIIDSANVDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDQLNPDFTLNKPRYQGASILVSQENFG
CGSSREHAPWALADFGLKVIIAPSFADIFYGNSINNGLLPVRLADAEVKQLMAEVEAKEGAEISVDLQALTVTSPSGSQF
HFEIAQSARHNLLNGLDAIGLTLSHADTIANYEANIPSWRR

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=198, Percent_Identity=64.1414141414141, Blast_Score=265, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6321429, Length=198, Percent_Identity=48.4848484848485, Blast_Score=185, Evalue=4e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004431
- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 22036; Mature: 22036

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPFISHTGLAVIIDSANVDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDQLNPDF
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCE
TLNKPRYQGASILVSQENFGCGSSREHAPWALADFGLKVIIAPSFADIFYGNSINNGLLP
EECCCCCCCCEEEEECCCCCCCCCCCCCCEEEHHCCEEEEEECCHHHHEECCCCCCCEEE
VRLADAEVKQLMAEVEAKEGAEISVDLQALTVTSPSGSQFHFEIAQSARHNLLNGLDAIG
EEECHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHC
LTLSHADTIANYEANIPSWRR
EEECCHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MQPFISHTGLAVIIDSANVDTDQIIPKQFLSKVTRDGFGVHLFHDWRYLDDAGDQLNPDF
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEECCCCCCCCCCE
TLNKPRYQGASILVSQENFGCGSSREHAPWALADFGLKVIIAPSFADIFYGNSINNGLLP
EECCCCCCCCEEEEECCCCCCCCCCCCCCEEEHHCCEEEEEECCHHHHEECCCCCCCEEE
VRLADAEVKQLMAEVEAKEGAEISVDLQALTVTSPSGSQFHFEIAQSARHNLLNGLDAIG
EEECHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHC
LTLSHADTIANYEANIPSWRR
EEECCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA