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The map label for this gene is gcvT [H]

Identifier: 157963119

GI number: 157963119

Start: 4046160

End: 4047254

Strand: Reverse

Name: gcvT [H]

Synonym: Spea_3303

Alternate gene names: 157963119

Gene position: 4047254-4046160 (Counterclockwise)

Preceding gene: 157963120

Following gene: 157963118

Centisome position: 78.21

GC content: 49.22

Gene sequence:

>1095_bases
ATGGCTAACAAAACTGTACTTTTTAACAAGCACCTAGAATCGAACGGCAAGATGGTAGACTTCCATGGTTGGGATATGCC
ACTAAACTACGGTTCACAAATCGAAGAACATCACGCAGTTCGTCAAGACGCTGGTATGTTCGACGTTTCACACATGACAG
TAGTCGACGTGATTGGTGATGACGCTTGTGCCTTTTTACGCAAACTGCTAGCCAACGATGTCGCTAAGCTTAAAGTGCCT
GGTAAGGCACTTTACGGCGGTATGCTTGACCACAATGGCGGCGTTATCGACGACCTTATCACTTATTACCTATCAGATAC
CGAGTACCGTATTGTTGTTAACTCAGCAACACGCGAAAAAGACCTCGCTTGGATAAACGAACAAGTTAAAGGCTTCAGCG
TCGAAGTGACCGAGCGTCCAGAGCTGGCGATGATCGCCGTTCAAGGCCCAAATGCTAAGGCAAAAGCGGCTACCGTATTT
AACGATGCGCAAAACGCAGCAATCGAAGGCATGAAGCCATTCTTTGGTGTACAAGCTGGCTCACTGTTTATCGCAACCAC
TGGCTACACTGGTGAAACTGGCTACGAAGTTATCGTACCAAATGATGAAGCCGAAGCACTATGGCAAGCTTTCCTAGATG
CGGGTATTAAGCCTTGTGGACTAGGTGCACGTGACACATTACGTCTCGAAGCGGGCATGAACCTTTACGGCCTAGATATG
GACGAAAGCGTTAACCCACTTGCAGCCAACATGGGCTGGACTGTGGCATGGGAGCCAGCGGAACGTGACTTTAACGGTCG
CCAAGCGCTAGAGAAGATTAAAGCAGAAGGTACAGACAAACTTGTTGGCCTAATCATGGACGCTAAAGGCGTTATCCGTC
ACGGCATGTCAGTGTTCTTTACCGACAGCGACGGCGTTGAGCAGCAAGGTACCATTACCAGCGGCACATTCTCGCCTACA
CTGGGTTACTCAATTGCCATGGCTCGCGTACCGCGCAGCATTGGTGACGTGGCTGAAGTAGAGATGCGTAAGAAGCGTGT
ACCGGTAAAAGTTATCGCTCCGTCATTTGTGCGCAATGGTAAGCAAGCCTTCTAA

Upstream 100 bases:

>100_bases
ATATATGTACCCTACAAAAGTACGCGATATAAGTACGCTATATAAAGTGTAAAGTGATGGCGGCAGTTGCAGACTAGAAG
ACCCCAAAGAAGGGATAGAA

Downstream 100 bases:

>100_bases
TTTTCAGGCTTAATACTGGCTATTTAGTCCTACTTTGTTGTCGCCAGTGCTCATTGATGCTTATCAACTGCGCGCTGGCT
CCTAAAATTAGAACTAAATC

Product: glycine cleavage system aminomethyltransferase T

Products: NA

Alternate protein names: Glycine cleavage system T protein [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MANKTVLFNKHLESNGKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGDDACAFLRKLLANDVAKLKVP
GKALYGGMLDHNGGVIDDLITYYLSDTEYRIVVNSATREKDLAWINEQVKGFSVEVTERPELAMIAVQGPNAKAKAATVF
NDAQNAAIEGMKPFFGVQAGSLFIATTGYTGETGYEVIVPNDEAEALWQAFLDAGIKPCGLGARDTLRLEAGMNLYGLDM
DESVNPLAANMGWTVAWEPAERDFNGRQALEKIKAEGTDKLVGLIMDAKGVIRHGMSVFFTDSDGVEQQGTITSGTFSPT
LGYSIAMARVPRSIGDVAEVEMRKKRVPVKVIAPSFVRNGKQAF

Sequences:

>Translated_364_residues
MANKTVLFNKHLESNGKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGDDACAFLRKLLANDVAKLKVP
GKALYGGMLDHNGGVIDDLITYYLSDTEYRIVVNSATREKDLAWINEQVKGFSVEVTERPELAMIAVQGPNAKAKAATVF
NDAQNAAIEGMKPFFGVQAGSLFIATTGYTGETGYEVIVPNDEAEALWQAFLDAGIKPCGLGARDTLRLEAGMNLYGLDM
DESVNPLAANMGWTVAWEPAERDFNGRQALEKIKAEGTDKLVGLIMDAKGVIRHGMSVFFTDSDGVEQQGTITSGTFSPT
LGYSIAMARVPRSIGDVAEVEMRKKRVPVKVIAPSFVRNGKQAF
>Mature_363_residues
ANKTVLFNKHLESNGKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGDDACAFLRKLLANDVAKLKVPG
KALYGGMLDHNGGVIDDLITYYLSDTEYRIVVNSATREKDLAWINEQVKGFSVEVTERPELAMIAVQGPNAKAKAATVFN
DAQNAAIEGMKPFFGVQAGSLFIATTGYTGETGYEVIVPNDEAEALWQAFLDAGIKPCGLGARDTLRLEAGMNLYGLDMD
ESVNPLAANMGWTVAWEPAERDFNGRQALEKIKAEGTDKLVGLIMDAKGVIRHGMSVFFTDSDGVEQQGTITSGTFSPTL
GYSIAMARVPRSIGDVAEVEMRKKRVPVKVIAPSFVRNGKQAF

Specific function: The glycine cleavage system catalyzes the degradation of glycine [H]

COG id: COG0404

COG function: function code E; Glycine cleavage system T protein (aminomethyltransferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gcvT family [H]

Homologues:

Organism=Homo sapiens, GI44662838, Length=361, Percent_Identity=33.2409972299169, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI257796258, Length=343, Percent_Identity=33.5276967930029, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI257796254, Length=354, Percent_Identity=29.6610169491525, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI257796256, Length=306, Percent_Identity=31.6993464052288, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI24797151, Length=341, Percent_Identity=30.2052785923754, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI197927446, Length=295, Percent_Identity=26.1016949152542, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI21361378, Length=295, Percent_Identity=26.1016949152542, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1789272, Length=361, Percent_Identity=66.7590027700831, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17560118, Length=378, Percent_Identity=33.3333333333333, Blast_Score=160, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI71994045, Length=355, Percent_Identity=24.7887323943662, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI71994052, Length=355, Percent_Identity=24.7887323943662, Blast_Score=78, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI32563613, Length=315, Percent_Identity=24.1269841269841, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320222, Length=375, Percent_Identity=33.0666666666667, Blast_Score=167, Evalue=4e-42,
Organism=Drosophila melanogaster, GI20129441, Length=377, Percent_Identity=33.9522546419098, Blast_Score=178, Evalue=6e-45,
Organism=Drosophila melanogaster, GI28571104, Length=270, Percent_Identity=25.9259259259259, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI20130091, Length=368, Percent_Identity=23.6413043478261, Blast_Score=71, Evalue=9e-13,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013977
- InterPro:   IPR006222
- InterPro:   IPR006223
- InterPro:   IPR022903 [H]

Pfam domain/function: PF01571 GCV_T; PF08669 GCV_T_C [H]

EC number: =2.1.2.10 [H]

Molecular weight: Translated: 39647; Mature: 39515

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANKTVLFNKHLESNGKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGD
CCCCEEEEECEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCEEEEEECCC
DACAFLRKLLANDVAKLKVPGKALYGGMLDHNGGVIDDLITYYLSDTEYRIVVNSATREK
HHHHHHHHHHHCCHHEEECCCHHHCCCEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCH
DLAWINEQVKGFSVEVTERPELAMIAVQGPNAKAKAATVFNDAQNAAIEGMKPFFGVQAG
HHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCEEEEEECCCHHHHHCCCCHHHCCCCC
SLFIATTGYTGETGYEVIVPNDEAEALWQAFLDAGIKPCGLGARDTLRLEAGMNLYGLDM
CEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECCC
DESVNPLAANMGWTVAWEPAERDFNGRQALEKIKAEGTDKLVGLIMDAKGVIRHGMSVFF
CCCCCCHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCEEEE
TDSDGVEQQGTITSGTFSPTLGYSIAMARVPRSIGDVAEVEMRKKRVPVKVIAPSFVRNG
ECCCCCCCCCCEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECHHHHCCC
KQAF
CCCC
>Mature Secondary Structure 
ANKTVLFNKHLESNGKMVDFHGWDMPLNYGSQIEEHHAVRQDAGMFDVSHMTVVDVIGD
CCCEEEEECEECCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCEEEEEECCC
DACAFLRKLLANDVAKLKVPGKALYGGMLDHNGGVIDDLITYYLSDTEYRIVVNSATREK
HHHHHHHHHHHCCHHEEECCCHHHCCCEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCH
DLAWINEQVKGFSVEVTERPELAMIAVQGPNAKAKAATVFNDAQNAAIEGMKPFFGVQAG
HHHHHHHHCCCEEEEEECCCCEEEEEEECCCCCCEEEEEECCCHHHHHCCCCHHHCCCCC
SLFIATTGYTGETGYEVIVPNDEAEALWQAFLDAGIKPCGLGARDTLRLEAGMNLYGLDM
CEEEEECCCCCCCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEECCC
DESVNPLAANMGWTVAWEPAERDFNGRQALEKIKAEGTDKLVGLIMDAKGVIRHGMSVFF
CCCCCCHHHCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCEEEE
TDSDGVEQQGTITSGTFSPTLGYSIAMARVPRSIGDVAEVEMRKKRVPVKVIAPSFVRNG
ECCCCCCCCCCEECCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCEEEECHHHHCCC
KQAF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA