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The map label for this gene is gcvH [H]

Identifier: 157963118

GI number: 157963118

Start: 4045342

End: 4045731

Strand: Reverse

Name: gcvH [H]

Synonym: Spea_3302

Alternate gene names: 157963118

Gene position: 4045731-4045342 (Counterclockwise)

Preceding gene: 157963119

Following gene: 157963117

Centisome position: 78.18

GC content: 48.72

Gene sequence:

>390_bases
ATGAGCAATATCCCAGCTGAACTTAAATATGCTTCTTCTCACGAGTGGATCCGTAAAGAAGAAGACGGTAGCTACACAGT
CGGTATCAGTGAGCACGCTCAAGAGCTTTTGGGTGACATGGTGTTCGTTGAGCTTCCAGAAGTTGGCGACGACGTTAACG
CTGGTGAAGACTGCGCAGTAGCAGAGTCAGTAAAAGCAGCATCAGACATCTACGCACCACTTTCAGGCGAAGTGGTTGCC
GTTAACGAAGCACTGGAAGATTCTCCAGAGCTAGTCAACAGCGATGCATTCGGTGACGGTTGGTTCTTCCGCGTAATGCC
TACCGACCTTGCAGAACTAGACAACTTGCTTGATGCCGAAGGTTATCAAGCAGTTATCGACGACGAATAA

Upstream 100 bases:

>100_bases
GATGTAAAAAAGGTGCAAAAGTTGATATAAGCCTTATAGGCAAATCAGTATAACGTCTTATTAAATACAGAATTAGAACA
TCGAATAAGGATTAAGACCA

Downstream 100 bases:

>100_bases
GCGTCGATGACTCACAAAGCCCCAACTCATCGCTGATTGGGGCTTTGTTCGTTATATGGCTTCTAAGCAAAATTAACAGG
CATGTAAGTGAGAATATTTT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSNIPAELKYASSHEWIRKEEDGSYTVGISEHAQELLGDMVFVELPEVGDDVNAGEDCAVAESVKAASDIYAPLSGEVVA
VNEALEDSPELVNSDAFGDGWFFRVMPTDLAELDNLLDAEGYQAVIDDE

Sequences:

>Translated_129_residues
MSNIPAELKYASSHEWIRKEEDGSYTVGISEHAQELLGDMVFVELPEVGDDVNAGEDCAVAESVKAASDIYAPLSGEVVA
VNEALEDSPELVNSDAFGDGWFFRVMPTDLAELDNLLDAEGYQAVIDDE
>Mature_128_residues
SNIPAELKYASSHEWIRKEEDGSYTVGISEHAQELLGDMVFVELPEVGDDVNAGEDCAVAESVKAASDIYAPLSGEVVAV
NEALEDSPELVNSDAFGDGWFFRVMPTDLAELDNLLDAEGYQAVIDDE

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=46.6666666666667, Blast_Score=115, Evalue=1e-26,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=46.6666666666667, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=70.5426356589147, Blast_Score=196, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17507493, Length=120, Percent_Identity=44.1666666666667, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17551294, Length=120, Percent_Identity=43.3333333333333, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=125, Percent_Identity=39.2, Blast_Score=103, Evalue=9e-24,
Organism=Drosophila melanogaster, GI17865652, Length=127, Percent_Identity=40.9448818897638, Blast_Score=110, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13970; Mature: 13839

Theoretical pI: Translated: 3.51; Mature: 3.51

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIPAELKYASSHEWIRKEEDGSYTVGISEHAQELLGDMVFVELPEVGDDVNAGEDCAV
CCCCCCCEEECCCCCCCEECCCCCEEEEHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHH
AESVKAASDIYAPLSGEVVAVNEALEDSPELVNSDAFGDGWFFRVMPTDLAELDNLLDAE
HHHHHHHHHHCCCCCCCEEEECCHHCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHCCC
GYQAVIDDE
CCEEEECCC
>Mature Secondary Structure 
SNIPAELKYASSHEWIRKEEDGSYTVGISEHAQELLGDMVFVELPEVGDDVNAGEDCAV
CCCCCCEEECCCCCCCEECCCCCEEEEHHHHHHHHHHCEEEEECCCCCCCCCCCCCCHH
AESVKAASDIYAPLSGEVVAVNEALEDSPELVNSDAFGDGWFFRVMPTDLAELDNLLDAE
HHHHHHHHHHCCCCCCCEEEECCHHCCCCHHCCCCCCCCCEEEEECCCCHHHHHHHHCCC
GYQAVIDDE
CCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA