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The map label for this gene is paaF [C]

Identifier: 157962600

GI number: 157962600

Start: 3395395

End: 3396573

Strand: Reverse

Name: paaF [C]

Synonym: Spea_2779

Alternate gene names: 157962600

Gene position: 3396573-3395395 (Counterclockwise)

Preceding gene: 157962601

Following gene: 157962599

Centisome position: 65.64

GC content: 50.04

Gene sequence:

>1179_bases
ATGAGCGAGTTGAATATGCAAGCAAACAGCGACCAAGTGCTGTTTAGTACGCTAACCACCGACTCTGGTAAGCAAGTTGG
CGTTGCGACACTGAATATTGAAAAAGCGCTCAATGCCCTAAACCTAAACATGGTGCAGGCGTTGACCGCTCAGCTGACTG
CATGGCGCAGTGATGACAATATTGTTGCAGTGGTACTCGACGGAGCCGGTGAAAAAGCCTTTTGCGCCGGTGGCGATGTA
CGCGCCATTTATCAAGCTTCGGTAAAAACGCCAGGTGAAGTCACTGATGCGGCAACCCAGTTCTTTAAACAAGAGTATGA
GCTCGATTATCTACTGCACACCTTTGGTAAACCTGTCGCCGTTTGGGGAGATGGCATAGTCATGGGCGGCGGCCTAGGCC
TGATGATAGGCGCAAGTCACCGTATCGTCACCGAGCGCTCGCGTATCGCCATGCCTGAGGTCACTATCGGTTTATACCCC
GATGTTGGCGGCAGTTATTTCTTAAATCGGATGCCGGGAAAATCCGGGCTATTTTTAGGAATGACGGCTTATAACATGAG
CGGCGCAGATGCCCATTATGTTGGCATAGGTAATCATTATCTGAACTTTGATGACAGAGCGGCATTCTTTGATGCCTTAA
CTGGCGTGAGTTGGAGTGCTAGCCAAAATGAGAATCATCAAGCCCTGCATAGCTTGCTCGATAAGATGCAAGAAAAATGC
AGCACGCCACTCGGCGCGAGCCAGCTTGAGCAAAACCGTGAGCTTATCGATGAGCTAATGACGGGCTCATTAGATGAGAT
CGTTGTTCGTGTTAGGGCTCGTATCAGTACACGTGTCACAGATCAGGATGAGCAAACGGCAATCGCGCACCCTTGGTTAG
TCAAAGCGATTAAGACCATGTTAGCGGGCAGTCCAATCAGCCTGAAACTTGTGCATTTGCAATCACAACTGAGTAAGGAT
TTGAGCCTAGCCGATTGTTTTAAGCTTGAACTTGGACTAAGTGTTAATTGCTGTGCCAAGGCAGATTTTGCCGAAGGTGT
GAGGGCGCTGCTTATCGATAAAGATCGCAATCCGCAGTGGCGCTTTGCCGATGTGGCGGCGATCCCCCAGGGCTACGCAG
AAGGTTTACTGCGATCCCCATGGGACGATGCCAGTCATCCGCTAAAAGGTTTGCACTAA

Upstream 100 bases:

>100_bases
TAGAGCGTGAACTGTTTGCTAATCTGTTTGACACCGAAGATCAGGCCGAAGGGGTTAATGCCTTCTTAAATAAGCGCAAA
GCGAATTGGAAGAATGGTTA

Downstream 100 bases:

>100_bases
CATTAAAAGATAAACGCTAGCGGTAAACTTAAAGATTGCCGCTAAATGGATTAAGTACCTAGCACTCACAAAGGTGAGTT
GGAGCGAATAATGAGTACTG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSELNMQANSDQVLFSTLTTDSGKQVGVATLNIEKALNALNLNMVQALTAQLTAWRSDDNIVAVVLDGAGEKAFCAGGDV
RAIYQASVKTPGEVTDAATQFFKQEYELDYLLHTFGKPVAVWGDGIVMGGGLGLMIGASHRIVTERSRIAMPEVTIGLYP
DVGGSYFLNRMPGKSGLFLGMTAYNMSGADAHYVGIGNHYLNFDDRAAFFDALTGVSWSASQNENHQALHSLLDKMQEKC
STPLGASQLEQNRELIDELMTGSLDEIVVRVRARISTRVTDQDEQTAIAHPWLVKAIKTMLAGSPISLKLVHLQSQLSKD
LSLADCFKLELGLSVNCCAKADFAEGVRALLIDKDRNPQWRFADVAAIPQGYAEGLLRSPWDDASHPLKGLH

Sequences:

>Translated_392_residues
MSELNMQANSDQVLFSTLTTDSGKQVGVATLNIEKALNALNLNMVQALTAQLTAWRSDDNIVAVVLDGAGEKAFCAGGDV
RAIYQASVKTPGEVTDAATQFFKQEYELDYLLHTFGKPVAVWGDGIVMGGGLGLMIGASHRIVTERSRIAMPEVTIGLYP
DVGGSYFLNRMPGKSGLFLGMTAYNMSGADAHYVGIGNHYLNFDDRAAFFDALTGVSWSASQNENHQALHSLLDKMQEKC
STPLGASQLEQNRELIDELMTGSLDEIVVRVRARISTRVTDQDEQTAIAHPWLVKAIKTMLAGSPISLKLVHLQSQLSKD
LSLADCFKLELGLSVNCCAKADFAEGVRALLIDKDRNPQWRFADVAAIPQGYAEGLLRSPWDDASHPLKGLH
>Mature_391_residues
SELNMQANSDQVLFSTLTTDSGKQVGVATLNIEKALNALNLNMVQALTAQLTAWRSDDNIVAVVLDGAGEKAFCAGGDVR
AIYQASVKTPGEVTDAATQFFKQEYELDYLLHTFGKPVAVWGDGIVMGGGLGLMIGASHRIVTERSRIAMPEVTIGLYPD
VGGSYFLNRMPGKSGLFLGMTAYNMSGADAHYVGIGNHYLNFDDRAAFFDALTGVSWSASQNENHQALHSLLDKMQEKCS
TPLGASQLEQNRELIDELMTGSLDEIVVRVRARISTRVTDQDEQTAIAHPWLVKAIKTMLAGSPISLKLVHLQSQLSKDL
SLADCFKLELGLSVNCCAKADFAEGVRALLIDKDRNPQWRFADVAAIPQGYAEGLLRSPWDDASHPLKGLH

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=381, Percent_Identity=32.8083989501312, Blast_Score=202, Evalue=3e-52,
Organism=Homo sapiens, GI37594469, Length=348, Percent_Identity=31.3218390804598, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI194097323, Length=177, Percent_Identity=31.638418079096, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787659, Length=161, Percent_Identity=28.5714285714286, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25144157, Length=354, Percent_Identity=35.5932203389831, Blast_Score=218, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI25144160, Length=354, Percent_Identity=35.5932203389831, Blast_Score=218, Evalue=3e-57,
Organism=Saccharomyces cerevisiae, GI6320241, Length=351, Percent_Identity=28.2051282051282, Blast_Score=139, Evalue=5e-34,
Organism=Drosophila melanogaster, GI28571729, Length=365, Percent_Identity=36.986301369863, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI28571730, Length=365, Percent_Identity=36.986301369863, Blast_Score=221, Evalue=5e-58,
Organism=Drosophila melanogaster, GI20129971, Length=199, Percent_Identity=29.6482412060301, Blast_Score=78, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24653477, Length=199, Percent_Identity=29.6482412060301, Blast_Score=78, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 42561; Mature: 42430

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELNMQANSDQVLFSTLTTDSGKQVGVATLNIEKALNALNLNMVQALTAQLTAWRSDDN
CCCCCCCCCCCEEEEEEEECCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IVAVVLDGAGEKAFCAGGDVRAIYQASVKTPGEVTDAATQFFKQEYELDYLLHTFGKPVA
EEEEEEECCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VWGDGIVMGGGLGLMIGASHRIVTERSRIAMPEVTIGLYPDVGGSYFLNRMPGKSGLFLG
EECCCEEEECCEEEEEECCCEEEECCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCEEEE
MTAYNMSGADAHYVGIGNHYLNFDDRAAFFDALTGVSWSASQNENHQALHSLLDKMQEKC
EEEECCCCCCEEEEECCCCEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
STPLGASQLEQNRELIDELMTGSLDEIVVRVRARISTRVTDQDEQTAIAHPWLVKAIKTM
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHH
LAGSPISLKLVHLQSQLSKDLSLADCFKLELGLSVNCCAKADFAEGVRALLIDKDRNPQW
HCCCCCEEEEEEHHHHHHCCCCHHHHHEEEECCEEEEECCCCHHHCCEEEEEECCCCCCE
RFADVAAIPQGYAEGLLRSPWDDASHPLKGLH
EEEHHHHCCCHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SELNMQANSDQVLFSTLTTDSGKQVGVATLNIEKALNALNLNMVQALTAQLTAWRSDDN
CCCCCCCCCCEEEEEEEECCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IVAVVLDGAGEKAFCAGGDVRAIYQASVKTPGEVTDAATQFFKQEYELDYLLHTFGKPVA
EEEEEEECCCCEEEECCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VWGDGIVMGGGLGLMIGASHRIVTERSRIAMPEVTIGLYPDVGGSYFLNRMPGKSGLFLG
EECCCEEEECCEEEEEECCCEEEECCCCCCCCCEEEEECCCCCCHHHHHCCCCCCCEEEE
MTAYNMSGADAHYVGIGNHYLNFDDRAAFFDALTGVSWSASQNENHQALHSLLDKMQEKC
EEEECCCCCCEEEEECCCCEECCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHH
STPLGASQLEQNRELIDELMTGSLDEIVVRVRARISTRVTDQDEQTAIAHPWLVKAIKTM
CCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCHHHHHHHHHH
LAGSPISLKLVHLQSQLSKDLSLADCFKLELGLSVNCCAKADFAEGVRALLIDKDRNPQW
HCCCCCEEEEEEHHHHHHCCCCHHHHHEEEECCEEEEECCCCHHHCCEEEEEECCCCCCE
RFADVAAIPQGYAEGLLRSPWDDASHPLKGLH
EEEHHHHCCCHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA