BacMap

Definition	Shewanella pealeana ATCC 700345 chromosome, complete genome.
Accession	NC_009901
Length	5,174,581

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The map label for this gene is paaF [C]

Identifier: 157962601

GI number: 157962601

Start: 3396573

End: 3397364

Strand: Reverse

Name: paaF [C]

Synonym: Spea_2780

Alternate gene names: 157962601

Gene position: 3397364-3396573 (Counterclockwise)

Preceding gene: 157962602

Following gene: 157962600

Centisome position: 65.65

GC content: 49.12

Gene sequence:

>792_bases
ATGACGTTAACAACAGAAAAGAGCTTATTGAAAGAGCAAATTGTTGGTCACACAGCGGTGATCAGCATCAACAATCCCCC
CGCTAACACTTGGACTGCCGATAGCCTGCAAGAGCTTAAGCATAAGGTATTAAGCCTCAATGAAAACAAAGAGGTTTATG
CCTTGGTGATTACTGGCGAAGGGAATAAGTTCTTCTCAGCTGGCGCCGATCTAAAACTGTTTGCCGATGGCGATAAGCAA
AATGCGGCGGATATGGCGCGTTACTTTGGTGAAGCCTTCGAAGCCTTAAGCAGCTTCCGCGGTGTCTCGATCGCAGCAAT
TAATGGTTATGCCATGGGCGGCGGTCTAGAAGTGGCACTCGCTTGTGATTTGCGTATTTGTGAAGAGCAGGCGCAACTGG
CGCTGCCAGAGGCTACCGTTGGTCTGCTGCCTTGTGCGGGCGGCACCCAGAACTTAACTGCGCTGGTGGGCGAAGGTTGG
GCTAAGCGTATGATCTTATGTGGTGAGCGAATTAACGCCGAGAAAGCCGAGAAGATAGGCCTAGTTGAAGAAGTGGTTGT
ACAAGGTAATGCCTTTAATACGGCAATGGCGCTGGCCGAAAAAGTAGCGAATCAAAGCCCAAGCAGTGTTAAAGCTTGTA
AAACATTGGTACAAGCTGGTCGCAGTATGCCAAGGGCACAGGCGCTACCACTAGAGCGTGAACTGTTTGCTAATCTGTTT
GACACCGAAGATCAGGCCGAAGGGGTTAATGCCTTCTTAAATAAGCGCAAAGCGAATTGGAAGAATGGTTAA

Upstream 100 bases:

>100_bases
TTGATTATCTCTCGCCGTTTATTGGATGAGAACGCAGGCGCGATTCTCTAGAGCCTTGGTATTCGGTTTAATTGATTTCA
GTTTAGTAAAAGGAGTTTTC

Downstream 100 bases:

>100_bases
TGAGCGAGTTGAATATGCAAGCAAACAGCGACCAAGTGCTGTTTAGTACGCTAACCACCGACTCTGGTAAGCAAGTTGGC
GTTGCGACACTGAATATTGA

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MTLTTEKSLLKEQIVGHTAVISINNPPANTWTADSLQELKHKVLSLNENKEVYALVITGEGNKFFSAGADLKLFADGDKQ
NAADMARYFGEAFEALSSFRGVSIAAINGYAMGGGLEVALACDLRICEEQAQLALPEATVGLLPCAGGTQNLTALVGEGW
AKRMILCGERINAEKAEKIGLVEEVVVQGNAFNTAMALAEKVANQSPSSVKACKTLVQAGRSMPRAQALPLERELFANLF
DTEDQAEGVNAFLNKRKANWKNG

Sequences:

>Translated_263_residues
MTLTTEKSLLKEQIVGHTAVISINNPPANTWTADSLQELKHKVLSLNENKEVYALVITGEGNKFFSAGADLKLFADGDKQ
NAADMARYFGEAFEALSSFRGVSIAAINGYAMGGGLEVALACDLRICEEQAQLALPEATVGLLPCAGGTQNLTALVGEGW
AKRMILCGERINAEKAEKIGLVEEVVVQGNAFNTAMALAEKVANQSPSSVKACKTLVQAGRSMPRAQALPLERELFANLF
DTEDQAEGVNAFLNKRKANWKNG
>Mature_262_residues
TLTTEKSLLKEQIVGHTAVISINNPPANTWTADSLQELKHKVLSLNENKEVYALVITGEGNKFFSAGADLKLFADGDKQN
AADMARYFGEAFEALSSFRGVSIAAINGYAMGGGLEVALACDLRICEEQAQLALPEATVGLLPCAGGTQNLTALVGEGWA
KRMILCGERINAEKAEKIGLVEEVVVQGNAFNTAMALAEKVANQSPSSVKACKTLVQAGRSMPRAQALPLERELFANLFD
TEDQAEGVNAFLNKRKANWKNG

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=244, Percent_Identity=38.9344262295082, Blast_Score=156, Evalue=2e-38,
Organism=Homo sapiens, GI68989263, Length=205, Percent_Identity=35.1219512195122, Blast_Score=112, Evalue=2e-25,
Organism=Homo sapiens, GI4502327, Length=237, Percent_Identity=29.957805907173, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI20127408, Length=178, Percent_Identity=33.7078651685393, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI157694516, Length=227, Percent_Identity=25.1101321585903, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI213417737, Length=227, Percent_Identity=25.1101321585903, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI261878539, Length=113, Percent_Identity=41.5929203539823, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI157694520, Length=207, Percent_Identity=25.6038647342995, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI150378541, Length=245, Percent_Identity=28.1632653061224, Blast_Score=81, Evalue=7e-16,
Organism=Homo sapiens, GI31542718, Length=250, Percent_Identity=27.2, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI37594469, Length=208, Percent_Identity=27.4038461538462, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI37594471, Length=208, Percent_Identity=27.4038461538462, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI70995211, Length=219, Percent_Identity=26.9406392694064, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI62530384, Length=195, Percent_Identity=26.1538461538462, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4757966, Length=203, Percent_Identity=23.6453201970443, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI51538581, Length=203, Percent_Identity=23.6453201970443, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI25453481, Length=203, Percent_Identity=23.6453201970443, Blast_Score=65, Evalue=5e-11,
Organism=Homo sapiens, GI51538573, Length=203, Percent_Identity=23.6453201970443, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787659, Length=243, Percent_Identity=37.4485596707819, Blast_Score=151, Evalue=5e-38,
Organism=Escherichia coli, GI221142681, Length=249, Percent_Identity=34.5381526104418, Blast_Score=144, Evalue=5e-36,
Organism=Escherichia coli, GI1788682, Length=240, Percent_Identity=35.4166666666667, Blast_Score=114, Evalue=9e-27,
Organism=Escherichia coli, GI1790281, Length=241, Percent_Identity=28.2157676348548, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87082183, Length=204, Percent_Identity=25.4901960784314, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=23.2931726907631, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1787660, Length=260, Percent_Identity=26.5384615384615, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=258, Percent_Identity=34.4961240310078, Blast_Score=142, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25145438, Length=247, Percent_Identity=35.2226720647773, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17540714, Length=221, Percent_Identity=29.4117647058824, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI17558304, Length=176, Percent_Identity=34.0909090909091, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=259, Percent_Identity=23.5521235521236, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25144276, Length=176, Percent_Identity=35.2272727272727, Blast_Score=80, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI17508951, Length=176, Percent_Identity=35.2272727272727, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508953, Length=176, Percent_Identity=35.2272727272727, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17560910, Length=164, Percent_Identity=32.9268292682927, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=261, Percent_Identity=24.1379310344828, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=188, Percent_Identity=26.5957446808511, Blast_Score=73, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI25144157, Length=180, Percent_Identity=27.2222222222222, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=180, Percent_Identity=27.2222222222222, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25141363, Length=132, Percent_Identity=31.8181818181818, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17535521, Length=251, Percent_Identity=26.2948207171315, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20129971, Length=257, Percent_Identity=34.6303501945525, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24653477, Length=257, Percent_Identity=34.6303501945525, Blast_Score=141, Evalue=5e-34,
Organism=Drosophila melanogaster, GI24653139, Length=248, Percent_Identity=30.241935483871, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24650670, Length=189, Percent_Identity=31.7460317460317, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922422, Length=243, Percent_Identity=22.6337448559671, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21357171, Length=145, Percent_Identity=33.1034482758621, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24654903, Length=193, Percent_Identity=29.0155440414508, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19921000, Length=247, Percent_Identity=29.5546558704453, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24583077, Length=247, Percent_Identity=29.5546558704453, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583079, Length=247, Percent_Identity=29.5546558704453, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583165, Length=167, Percent_Identity=26.3473053892216, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19920382, Length=191, Percent_Identity=27.2251308900524, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI19921018, Length=167, Percent_Identity=25.1497005988024, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28147; Mature: 28016

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTTEKSLLKEQIVGHTAVISINNPPANTWTADSLQELKHKVLSLNENKEVYALVITGE
CCCCCHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECC
GNKFFSAGADLKLFADGDKQNAADMARYFGEAFEALSSFRGVSIAAINGYAMGGGLEVAL
CCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCEEEEE
ACDLRICEEQAQLALPEATVGLLPCAGGTQNLTALVGEGWAKRMILCGERINAEKAEKIG
EECHHHHHHHHHHCCCCHHEEEEECCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHH
LVEEVVVQGNAFNTAMALAEKVANQSPSSVKACKTLVQAGRSMPRAQALPLERELFANLF
HHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHH
DTEDQAEGVNAFLNKRKANWKNG
CCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TLTTEKSLLKEQIVGHTAVISINNPPANTWTADSLQELKHKVLSLNENKEVYALVITGE
CCCCHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEEECC
GNKFFSAGADLKLFADGDKQNAADMARYFGEAFEALSSFRGVSIAAINGYAMGGGLEVAL
CCEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCEEECCCEEEEE
ACDLRICEEQAQLALPEATVGLLPCAGGTQNLTALVGEGWAKRMILCGERINAEKAEKIG
EECHHHHHHHHHHCCCCHHEEEEECCCCCCHHHHHHCCHHHHHHHHHCCCCCHHHHHHHH
LVEEVVVQGNAFNTAMALAEKVANQSPSSVKACKTLVQAGRSMPRAQALPLERELFANLF
HHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHCCCHHHHHHHHHH
DTEDQAEGVNAFLNKRKANWKNG
CCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA