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The map label for this gene is mmsB [H]

Identifier: 157962599

GI number: 157962599

Start: 3394375

End: 3395304

Strand: Reverse

Name: mmsB [H]

Synonym: Spea_2778

Alternate gene names: 157962599

Gene position: 3395304-3394375 (Counterclockwise)

Preceding gene: 157962600

Following gene: 157962598

Centisome position: 65.62

GC content: 49.35

Gene sequence:

>930_bases
ATGAGTACTGTTGCATTTATTGGTTTAGGAAACATGGGTGGCCCGATGGCGGCCAATCTGATTAAAGCGGGCTTTACGGT
TAAAGTGTTCGATCTGGTGCCTGAAGCGATGCAGGCATTAGCTGAGCAAGGGGCACTTACAGCAACAAGCGCTTGCGGCG
CCGCAGCGGGCGCGGACATAGTGGTGACCATGCTGCCTGCTGGCAAACATGTGCGTAGTCTTTATGTATCAAGTGATGCA
AACGATGATACAAAAAAAGGGCTTATCGATGTGGTGGCTGCGGGGACCCTCTTAATCGATTGTTCAACCATAGATGCAGA
CAGTGCGCGCTTTGTTGCCGAGCATGCGGCTAACAAGGGCTTGGAGTTTATCGATGCGCCAGTATCTGGCGGCACGGCAG
GTGCGGCGGCTGGTACATTGACCTTTATTTGTGGTGGCAGCGATAAGGCTTTTGAACTGGCACAGGGTGTATTAAATGCC
ATGGGGGGGAATATCTTCCACGCTGGCGGTCCTGGTGCTGGGCAAGTGGCTAAAATATGTAATAATATGCTGCTTTCGGT
ACTGATGGTTGCCACATCAGAATCATTGCAGATGGGAGTCGATCATGGGCTCGACCCAAAAGTGCTTTCCGATATTATGA
AAGTCAGCAGTGGTGGTAATTGGACGTTAGAGAAATATAATCCTTGTCCAGATGTGATGGATTCGGTGCCCTCTTCAAAT
GGATATCAAGGCGGCTTTATGGTCGACTTGATGGTGAAGGACTTAGGGCTTTCGCAAGAGGCGGCCTTGTTATCGAATTC
GAGTACGCCAATGGGCGCGTTAGCACGTAGCCTCTATGTGAATCATGCGAAACAAGGTAACGGTAAGCGAGATTTTTCGA
GTATTTTTGAGCTGTTTGCTCAGTCAGAACAGATAAAACACAAAGGGTGA

Upstream 100 bases:

>100_bases
TTTGCACTAACATTAAAAGATAAACGCTAGCGGTAAACTTAAAGATTGCCGCTAAATGGATTAAGTACCTAGCACTCACA
AAGGTGAGTTGGAGCGAATA

Downstream 100 bases:

>100_bases
ACCAATGGATTTAAAAGATAAGGTTGTTGTTATTACTGGTGGTGCAGGTGGACTAGGTTACGCCATGGCGGAGAATTTAG
CGGCTGCAGGTGCCAAGCTA

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MSTVAFIGLGNMGGPMAANLIKAGFTVKVFDLVPEAMQALAEQGALTATSACGAAAGADIVVTMLPAGKHVRSLYVSSDA
NDDTKKGLIDVVAAGTLLIDCSTIDADSARFVAEHAANKGLEFIDAPVSGGTAGAAAGTLTFICGGSDKAFELAQGVLNA
MGGNIFHAGGPGAGQVAKICNNMLLSVLMVATSESLQMGVDHGLDPKVLSDIMKVSSGGNWTLEKYNPCPDVMDSVPSSN
GYQGGFMVDLMVKDLGLSQEAALLSNSSTPMGALARSLYVNHAKQGNGKRDFSSIFELFAQSEQIKHKG

Sequences:

>Translated_309_residues
MSTVAFIGLGNMGGPMAANLIKAGFTVKVFDLVPEAMQALAEQGALTATSACGAAAGADIVVTMLPAGKHVRSLYVSSDA
NDDTKKGLIDVVAAGTLLIDCSTIDADSARFVAEHAANKGLEFIDAPVSGGTAGAAAGTLTFICGGSDKAFELAQGVLNA
MGGNIFHAGGPGAGQVAKICNNMLLSVLMVATSESLQMGVDHGLDPKVLSDIMKVSSGGNWTLEKYNPCPDVMDSVPSSN
GYQGGFMVDLMVKDLGLSQEAALLSNSSTPMGALARSLYVNHAKQGNGKRDFSSIFELFAQSEQIKHKG
>Mature_308_residues
STVAFIGLGNMGGPMAANLIKAGFTVKVFDLVPEAMQALAEQGALTATSACGAAAGADIVVTMLPAGKHVRSLYVSSDAN
DDTKKGLIDVVAAGTLLIDCSTIDADSARFVAEHAANKGLEFIDAPVSGGTAGAAAGTLTFICGGSDKAFELAQGVLNAM
GGNIFHAGGPGAGQVAKICNNMLLSVLMVATSESLQMGVDHGLDPKVLSDIMKVSSGGNWTLEKYNPCPDVMDSVPSSNG
YQGGFMVDLMVKDLGLSQEAALLSNSSTPMGALARSLYVNHAKQGNGKRDFSSIFELFAQSEQIKHKG

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=302, Percent_Identity=43.7086092715232, Blast_Score=259, Evalue=2e-69,
Organism=Homo sapiens, GI40556376, Length=296, Percent_Identity=23.9864864864865, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI145693186, Length=303, Percent_Identity=32.6732673267327, Blast_Score=155, Evalue=3e-39,
Organism=Escherichia coli, GI1790315, Length=298, Percent_Identity=32.8859060402685, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1786719, Length=292, Percent_Identity=29.7945205479452, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789092, Length=299, Percent_Identity=29.7658862876254, Blast_Score=106, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17557316, Length=303, Percent_Identity=41.5841584158416, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24655230, Length=300, Percent_Identity=41, Blast_Score=224, Evalue=5e-59,
Organism=Drosophila melanogaster, GI19922568, Length=300, Percent_Identity=41, Blast_Score=224, Evalue=5e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 31638; Mature: 31507

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVAFIGLGNMGGPMAANLIKAGFTVKVFDLVPEAMQALAEQGALTATSACGAAAGADI
CCEEEEEEECCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCE
VVTMLPAGKHVRSLYVSSDANDDTKKGLIDVVAAGTLLIDCSTIDADSARFVAEHAANKG
EEEECCCCHHHHEEEECCCCCCCHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHCCC
LEFIDAPVSGGTAGAAAGTLTFICGGSDKAFELAQGVLNAMGGNIFHAGGPGAGQVAKIC
CEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
NNMLLSVLMVATSESLQMGVDHGLDPKVLSDIMKVSSGGNWTLEKYNPCPDVMDSVPSSN
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCCC
GYQGGFMVDLMVKDLGLSQEAALLSNSSTPMGALARSLYVNHAKQGNGKRDFSSIFELFA
CCCCCEEEEEHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
QSEQIKHKG
HHHHHCCCC
>Mature Secondary Structure 
STVAFIGLGNMGGPMAANLIKAGFTVKVFDLVPEAMQALAEQGALTATSACGAAAGADI
CEEEEEEECCCCCHHHHHHHHCCCEEEHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCE
VVTMLPAGKHVRSLYVSSDANDDTKKGLIDVVAAGTLLIDCSTIDADSARFVAEHAANKG
EEEECCCCHHHHEEEECCCCCCCHHHHHHHHHHHCEEEEEECCCCCCHHHHHHHHHHCCC
LEFIDAPVSGGTAGAAAGTLTFICGGSDKAFELAQGVLNAMGGNIFHAGGPGAGQVAKIC
CEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHH
NNMLLSVLMVATSESLQMGVDHGLDPKVLSDIMKVSSGGNWTLEKYNPCPDVMDSVPSSN
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCHHHHHCCCCCC
GYQGGFMVDLMVKDLGLSQEAALLSNSSTPMGALARSLYVNHAKQGNGKRDFSSIFELFA
CCCCCEEEEEHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
QSEQIKHKG
HHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339433; 10984043 [H]