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The map label for this gene is thiF [H]

Identifier: 157962202

GI number: 157962202

Start: 2902480

End: 2903316

Strand: Reverse

Name: thiF [H]

Synonym: Spea_2381

Alternate gene names: 157962202

Gene position: 2903316-2902480 (Counterclockwise)

Preceding gene: 157962203

Following gene: 157962201

Centisome position: 56.11

GC content: 47.67

Gene sequence:

>837_bases
ATGAGAAATAGTATGTCACTCACTGATAGTGATTTTTTACGATACTCGCGTCAGGTGTTATTGCCTGAAGTTGGCGAGGC
TGGTCAGATTGCCATTTCCAAGTCTCATGTTGTTATCATCGGCGTTGGTGGCTTGGGCAATCTAGTTGCTCAGCAGCTAG
CCGCCGCTGGTGTGGGTCATATCACTCTTGTTGATGGAGATAGGGTAGAGCTGTCAAATTTACCCAGACAATTGCTATTT
GACGATAGCGATATCGGTAACAATAAAGCGCTGGTGGCCAAGGATAAACTCAGCCGTGCCTACACTCAAACTCAGCTTAT
CGCGGTGAGTGAGCATTTAACTCAAGACAATATCAGTCAGGTGTTAACACTTGATGAGCCCTATGCTCAGCTACTCGACT
GCACTGACAACTTCGAAACCCGACAGCGAGTCAATAGGTTTGCGATCGAGAGCCGTACCCCTTTGGTGTCGGCATCGGCG
GCAAATTTTCAGGGACAGCTGCTCAATATCAATCAACAGCATAATCCACAATCTGGTTGTTATCACTGTTTGTATCCAGC
TGATATGTCGGTGAATCAAACTTGCCAAACTGTCGGTGTTTTGGGGCCTATGGTCGCAGCGTTAGCGTCAATGCAGGCAT
TAATGGCGCTGCAACAATTATTAGGAGATAGGCAGACGGTAGGTAAGCTGTTTCGCTTCGATGGCAAATCCTTTAGTTGG
CGAGAAGCCCGTTTACCGCGTAGCCCCGAGTGCGTGATCTGTGGGGACGTTCAAGGTCTATCTGGTGCCGAAAAAAACTG
CGGCAAGTTACCGTCTACGGAGAAAGAATATGTATAA

Upstream 100 bases:

>100_bases
GTTAAAGCAACGGGTGTGGATGACATCGCAGTGGTGCGAGCTTTGACTGAGGCCGATGATCCCACTGCGGCGTTTACGGC
TCTGAATAGGGCATGGGCTG

Downstream 100 bases:

>100_bases
AGGTAATGACAGCATTGCTATCAGTATCAATGATGAGAGCAAGCAAGTTGCGATTAACTCAAGCGTGCAAAGTGTGATTG
AAATGTATGCCGAGGTGAGA

Product: UBA/THIF-type NAD/FAD-binding protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MRNSMSLTDSDFLRYSRQVLLPEVGEAGQIAISKSHVVIIGVGGLGNLVAQQLAAAGVGHITLVDGDRVELSNLPRQLLF
DDSDIGNNKALVAKDKLSRAYTQTQLIAVSEHLTQDNISQVLTLDEPYAQLLDCTDNFETRQRVNRFAIESRTPLVSASA
ANFQGQLLNINQQHNPQSGCYHCLYPADMSVNQTCQTVGVLGPMVAALASMQALMALQQLLGDRQTVGKLFRFDGKSFSW
REARLPRSPECVICGDVQGLSGAEKNCGKLPSTEKEYV

Sequences:

>Translated_278_residues
MRNSMSLTDSDFLRYSRQVLLPEVGEAGQIAISKSHVVIIGVGGLGNLVAQQLAAAGVGHITLVDGDRVELSNLPRQLLF
DDSDIGNNKALVAKDKLSRAYTQTQLIAVSEHLTQDNISQVLTLDEPYAQLLDCTDNFETRQRVNRFAIESRTPLVSASA
ANFQGQLLNINQQHNPQSGCYHCLYPADMSVNQTCQTVGVLGPMVAALASMQALMALQQLLGDRQTVGKLFRFDGKSFSW
REARLPRSPECVICGDVQGLSGAEKNCGKLPSTEKEYV
>Mature_278_residues
MRNSMSLTDSDFLRYSRQVLLPEVGEAGQIAISKSHVVIIGVGGLGNLVAQQLAAAGVGHITLVDGDRVELSNLPRQLLF
DDSDIGNNKALVAKDKLSRAYTQTQLIAVSEHLTQDNISQVLTLDEPYAQLLDCTDNFETRQRVNRFAIESRTPLVSASA
ANFQGQLLNINQQHNPQSGCYHCLYPADMSVNQTCQTVGVLGPMVAALASMQALMALQQLLGDRQTVGKLFRFDGKSFSW
REARLPRSPECVICGDVQGLSGAEKNCGKLPSTEKEYV

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein ThiS [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=181, Percent_Identity=41.9889502762431, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI4885649, Length=153, Percent_Identity=28.7581699346405, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI87082356, Length=249, Percent_Identity=41.3654618473896, Blast_Score=176, Evalue=1e-45,
Organism=Escherichia coli, GI1787048, Length=250, Percent_Identity=35.6, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI1789177, Length=160, Percent_Identity=26.875, Blast_Score=73, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540406, Length=254, Percent_Identity=37.4015748031496, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI193203301, Length=163, Percent_Identity=26.9938650306748, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=219, Percent_Identity=35.6164383561644, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321965, Length=211, Percent_Identity=29.8578199052133, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6320598, Length=165, Percent_Identity=26.6666666666667, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24582879, Length=276, Percent_Identity=36.5942028985507, Blast_Score=151, Evalue=5e-37,
Organism=Drosophila melanogaster, GI24660640, Length=155, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012731
- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 30295; Mature: 30295

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNSMSLTDSDFLRYSRQVLLPEVGEAGQIAISKSHVVIIGVGGLGNLVAQQLAAAGVGH
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHCCCCE
ITLVDGDRVELSNLPRQLLFDDSDIGNNKALVAKDKLSRAYTQTQLIAVSEHLTQDNISQ
EEEECCCEEEHHHCCHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHH
VLTLDEPYAQLLDCTDNFETRQRVNRFAIESRTPLVSASAANFQGQLLNINQQHNPQSGC
EEECCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCCCCCCC
YHCLYPADMSVNQTCQTVGVLGPMVAALASMQALMALQQLLGDRQTVGKLFRFDGKSFSW
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
REARLPRSPECVICGDVQGLSGAEKNCGKLPSTEKEYV
HHHCCCCCCCEEEEECCCCCCCCCHHCCCCCCCCCCCC
>Mature Secondary Structure
MRNSMSLTDSDFLRYSRQVLLPEVGEAGQIAISKSHVVIIGVGGLGNLVAQQLAAAGVGH
CCCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEECCHHHHHHHHHHHCCCCE
ITLVDGDRVELSNLPRQLLFDDSDIGNNKALVAKDKLSRAYTQTQLIAVSEHLTQDNISQ
EEEECCCEEEHHHCCHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHH
VLTLDEPYAQLLDCTDNFETRQRVNRFAIESRTPLVSASAANFQGQLLNINQQHNPQSGC
EEECCCHHHHHHCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEECCCCCCCCCCC
YHCLYPADMSVNQTCQTVGVLGPMVAALASMQALMALQQLLGDRQTVGKLFRFDGKSFSW
EEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCC
REARLPRSPECVICGDVQGLSGAEKNCGKLPSTEKEYV
HHHCCCCCCCEEEEECCCCCCCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 9632726; 10082377 [H]