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The map label for this gene is gap [H]

Identifier: 157961927

GI number: 157961927

Start: 2548825

End: 2550261

Strand: Direct

Name: gap [H]

Synonym: Spea_2106

Alternate gene names: 157961927

Gene position: 2548825-2550261 (Clockwise)

Preceding gene: 157961924

Following gene: 157961934

Centisome position: 49.26

GC content: 43.63

Gene sequence:

>1437_bases
ATGAGCGCTGATAAACACCTACAAAGTTGGCAAGAACGATTCGAAATGGCTGAGGCTATGCAGCCATTACTTGGTAAGTT
ATACCGTAACCAAGGTGTAGAAGTCGTCGTCTATGGCAAACCTCTACTGAATGCCTCTACAATCGAAATCATTAAATCGC
ACCGTTTAGTGCGTCGCCACGTTGGTGAAAAACTAAGACTACGTGAAAGCTTTCCATTTGTTGAGGCTTTAAGCAAGTTA
GCGGTAAAACAGTGCAAAGTCGATATCGGTAAATTAGCTGTTAATTACTGGCGCGAATACCCTGATGCGAGTCAAATTGA
GGCTTATATGAGCCGTGAACTTGCAGATGCTATCACTAACGAAGACGACGAAGCCGCTAGAGATGTTGTCCTATACGGTT
TTGGTCGTATTGGTCGTCTATTAGCACGTTTGCTGATTGAAAGAACAGGTGTTAGTAACAAGCTTAGACTACGTGCAATC
GTACTGCGCGGTGGCCGTAAAGGTGATTTAGAAAAGCGTGCAAGCTTACTACGTCGTGACTCGGTACATGGTCCATTCAA
CGGTTCAGTTGAAGTCGACGAAGAAAACAACGCAATTATCGCTAACGGTACCTACATTCAGGTTATCTACGCGAACTCGC
CAGACGAAGTGGATTACACTAAATTCGGTATTTCAAATGCGCTTGTAGTTGATAACACCGGTATTTGGAAAGATGAAGCG
GGCCTTGGTTTACACCTTAAATCACCAGGTGCAAGCAAAGTATTGCTTACTGCTCCAGCAAAAGGTGCAATTAAGAACGT
AGTTTATGGTGTAAACGAGCAAGACATCCTTGACGAAGATACGATTGTATCAGCTGCAAGCTGTACCACTAACGCGATTA
CACCAGTACTTAAAGCGGTTAATGACAAGTACGGTATCGAAAATGGTCACGTGGAAACAATCCACTCATATACTAACGAC
CAAAACCTAATCGATAACTATCACAGTGCAGATCGCCGTGGACGTAGTGCTCCATTAAACATGGTAATTACTGAAACAGG
CGCAGCTAAAGCAGTATCTAAGGCGTTACCTGTGCTAGAAGGTAAGCTAACAGGCAACGCGATTCGTGTGCCTACACCAA
ACGTTTCTATGGCGATTATTAGCTTAAACCTGAACGGTGAGACTAATAAAGAAGAGCTTAACGAATACATAAAAGATATG
GCGCTGCAGTCTGATTTGCAAAACCAGATTGATTACACTGATTCGACAGAAATCGTTTCGAGCGACCTTGTGGGTTCACG
TTACGCAGGTGTTGTTGATTCGCAAGCGACCATTGCTGAAGGTAAGCGTGCAATCCTTTACGTATGGTATGACAATGAAT
TTGGCTATAGCTGCCAGGTTGTTGGTGTTATGCAAAAGATGCTTGGCCTTAACTACCAGTCATTACCACATGGCTAA

Upstream 100 bases:

>100_bases
GGAAAGACACCCTATATAACGTTAGATTATTGGTCTTTTCTTTTTGTTACTCAAACATTTCTGATGAAAAAAGCAGAAAA
GTGAGATAAAGGATCTGTTA

Downstream 100 bases:

>100_bases
TTTGTTATAACTCTACGCTTTCTGATAAAACGCCTCTTACTGTATAGAGGCGTTTTTGTTTTTAGAACTAGCATTATCCA
ATAACATTACTTGCTTATTC

Product: glyceraldehyde-3-phosphate dehydrogenase

Products: NA

Alternate protein names: GAPDH [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MSADKHLQSWQERFEMAEAMQPLLGKLYRNQGVEVVVYGKPLLNASTIEIIKSHRLVRRHVGEKLRLRESFPFVEALSKL
AVKQCKVDIGKLAVNYWREYPDASQIEAYMSRELADAITNEDDEAARDVVLYGFGRIGRLLARLLIERTGVSNKLRLRAI
VLRGGRKGDLEKRASLLRRDSVHGPFNGSVEVDEENNAIIANGTYIQVIYANSPDEVDYTKFGISNALVVDNTGIWKDEA
GLGLHLKSPGASKVLLTAPAKGAIKNVVYGVNEQDILDEDTIVSAASCTTNAITPVLKAVNDKYGIENGHVETIHSYTND
QNLIDNYHSADRRGRSAPLNMVITETGAAKAVSKALPVLEGKLTGNAIRVPTPNVSMAIISLNLNGETNKEELNEYIKDM
ALQSDLQNQIDYTDSTEIVSSDLVGSRYAGVVDSQATIAEGKRAILYVWYDNEFGYSCQVVGVMQKMLGLNYQSLPHG

Sequences:

>Translated_478_residues
MSADKHLQSWQERFEMAEAMQPLLGKLYRNQGVEVVVYGKPLLNASTIEIIKSHRLVRRHVGEKLRLRESFPFVEALSKL
AVKQCKVDIGKLAVNYWREYPDASQIEAYMSRELADAITNEDDEAARDVVLYGFGRIGRLLARLLIERTGVSNKLRLRAI
VLRGGRKGDLEKRASLLRRDSVHGPFNGSVEVDEENNAIIANGTYIQVIYANSPDEVDYTKFGISNALVVDNTGIWKDEA
GLGLHLKSPGASKVLLTAPAKGAIKNVVYGVNEQDILDEDTIVSAASCTTNAITPVLKAVNDKYGIENGHVETIHSYTND
QNLIDNYHSADRRGRSAPLNMVITETGAAKAVSKALPVLEGKLTGNAIRVPTPNVSMAIISLNLNGETNKEELNEYIKDM
ALQSDLQNQIDYTDSTEIVSSDLVGSRYAGVVDSQATIAEGKRAILYVWYDNEFGYSCQVVGVMQKMLGLNYQSLPHG
>Mature_477_residues
SADKHLQSWQERFEMAEAMQPLLGKLYRNQGVEVVVYGKPLLNASTIEIIKSHRLVRRHVGEKLRLRESFPFVEALSKLA
VKQCKVDIGKLAVNYWREYPDASQIEAYMSRELADAITNEDDEAARDVVLYGFGRIGRLLARLLIERTGVSNKLRLRAIV
LRGGRKGDLEKRASLLRRDSVHGPFNGSVEVDEENNAIIANGTYIQVIYANSPDEVDYTKFGISNALVVDNTGIWKDEAG
LGLHLKSPGASKVLLTAPAKGAIKNVVYGVNEQDILDEDTIVSAASCTTNAITPVLKAVNDKYGIENGHVETIHSYTNDQ
NLIDNYHSADRRGRSAPLNMVITETGAAKAVSKALPVLEGKLTGNAIRVPTPNVSMAIISLNLNGETNKEELNEYIKDMA
LQSDLQNQIDYTDSTEIVSSDLVGSRYAGVVDSQATIAEGKRAILYVWYDNEFGYSCQVVGVMQKMLGLNYQSLPHG

Specific function: Second phase of glycolysis; first step. [C]

COG id: COG0057

COG function: function code G; Glyceraldehyde-3-phosphate dehydrogenase/erythrose-4-phosphate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI7657116, Length=339, Percent_Identity=36.283185840708, Blast_Score=186, Evalue=6e-47,
Organism=Homo sapiens, GI7669492, Length=337, Percent_Identity=35.9050445103858, Blast_Score=179, Evalue=6e-45,
Organism=Escherichia coli, GI1788079, Length=339, Percent_Identity=36.283185840708, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789295, Length=347, Percent_Identity=32.8530259365994, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17534677, Length=338, Percent_Identity=34.9112426035503, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17534679, Length=338, Percent_Identity=34.6153846153846, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17568413, Length=340, Percent_Identity=35.5882352941176, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI32566163, Length=340, Percent_Identity=35.5882352941176, Blast_Score=168, Evalue=6e-42,
Organism=Saccharomyces cerevisiae, GI6322468, Length=341, Percent_Identity=36.950146627566, Blast_Score=199, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6321631, Length=342, Percent_Identity=37.4269005847953, Blast_Score=198, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6322409, Length=339, Percent_Identity=36.5781710914454, Blast_Score=194, Evalue=3e-50,
Organism=Drosophila melanogaster, GI19922412, Length=331, Percent_Identity=37.7643504531722, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI17933600, Length=337, Percent_Identity=35.3115727002967, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI18110149, Length=337, Percent_Identity=35.3115727002967, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI85725000, Length=337, Percent_Identity=35.6083086053413, Blast_Score=181, Evalue=1e-45,
Organism=Drosophila melanogaster, GI22023983, Length=337, Percent_Identity=35.6083086053413, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020831
- InterPro:   IPR020830
- InterPro:   IPR020829
- InterPro:   IPR020828
- InterPro:   IPR006424
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02800 Gp_dh_C; PF00044 Gp_dh_N [H]

EC number: =1.2.1.12 [H]

Molecular weight: Translated: 52714; Mature: 52583

Theoretical pI: Translated: 6.09; Mature: 6.09

Prosite motif: PS00071 GAPDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADKHLQSWQERFEMAEAMQPLLGKLYRNQGVEVVVYGKPLLNASTIEIIKSHRLVRRH
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
VGEKLRLRESFPFVEALSKLAVKQCKVDIGKLAVNYWREYPDASQIEAYMSRELADAITN
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
EDDEAARDVVLYGFGRIGRLLARLLIERTGVSNKLRLRAIVLRGGRKGDLEKRASLLRRD
CCCHHHHHEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHC
SVHGPFNGSVEVDEENNAIIANGTYIQVIYANSPDEVDYTKFGISNALVVDNTGIWKDEA
CCCCCCCCCEEEECCCCEEEECCCEEEEEECCCCCCCCHHHHCCCCEEEEECCCCCCCCC
GLGLHLKSPGASKVLLTAPAKGAIKNVVYGVNEQDILDEDTIVSAASCTTNAITPVLKAV
CCEEEECCCCCCEEEEECCCCHHHHHHHCCCCCHHCCCCCHHEEHHHCCHHHHHHHHHHH
NDKYGIENGHVETIHSYTNDQNLIDNYHSADRRGRSAPLNMVITETGAAKAVSKALPVLE
HHHCCCCCCCEEEEHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHC
GKLTGNAIRVPTPNVSMAIISLNLNGETNKEELNEYIKDMALQSDLQNQIDYTDSTEIVS
CEECCCEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SDLVGSRYAGVVDSQATIAEGKRAILYVWYDNEFGYSCQVVGVMQKMLGLNYQSLPHG
HHCCCCHHCCCCCCCHHHHCCCEEEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SADKHLQSWQERFEMAEAMQPLLGKLYRNQGVEVVVYGKPLLNASTIEIIKSHRLVRRH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
VGEKLRLRESFPFVEALSKLAVKQCKVDIGKLAVNYWREYPDASQIEAYMSRELADAITN
CCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
EDDEAARDVVLYGFGRIGRLLARLLIERTGVSNKLRLRAIVLRGGRKGDLEKRASLLRRD
CCCHHHHHEEEECHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHC
SVHGPFNGSVEVDEENNAIIANGTYIQVIYANSPDEVDYTKFGISNALVVDNTGIWKDEA
CCCCCCCCCEEEECCCCEEEECCCEEEEEECCCCCCCCHHHHCCCCEEEEECCCCCCCCC
GLGLHLKSPGASKVLLTAPAKGAIKNVVYGVNEQDILDEDTIVSAASCTTNAITPVLKAV
CCEEEECCCCCCEEEEECCCCHHHHHHHCCCCCHHCCCCCHHEEHHHCCHHHHHHHHHHH
NDKYGIENGHVETIHSYTNDQNLIDNYHSADRRGRSAPLNMVITETGAAKAVSKALPVLE
HHHCCCCCCCEEEEHHHCCCCHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHC
GKLTGNAIRVPTPNVSMAIISLNLNGETNKEELNEYIKDMALQSDLQNQIDYTDSTEIVS
CEECCCEEECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
SDLVGSRYAGVVDSQATIAEGKRAILYVWYDNEFGYSCQVVGVMQKMLGLNYQSLPHG
HHCCCCHHCCCCCCCHHHHCCCEEEEEEEECCCCCCEEHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8508805; 10360571; 2401296; 7877172 [H]