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The map label for this gene is rihA [H]

Identifier: 157961687

GI number: 157961687

Start: 2270984

End: 2271952

Strand: Reverse

Name: rihA [H]

Synonym: Spea_1863

Alternate gene names: 157961687

Gene position: 2271952-2270984 (Counterclockwise)

Preceding gene: 157961688

Following gene: 157961685

Centisome position: 43.91

GC content: 48.92

Gene sequence:

>969_bases
ATGAGCCCTACTCAAGCAAAGCAGCAATCACAAAAAGTTACTCCTATTATTCTCGACTGCGATCCCGGCCATGATGACGC
AATTTCATTAATCCTAGCCATGAGCAGTAAGGCACTTAAGCCATTAGCGGTCACCACTAGTGCCGGTAATCAAACCCCCG
ATAAGACCTTAAACAATGCGCTGCGCATTTTAACCTTGCTCGAGCGCCACGATATTCCTGTCGCAGCTGGCGCACCTAAG
CCTTTAGCCCGAGAGCTGATTATTGCCGACAATGTCCATGGTGAGAGTGGTCTAGACGGTCCTAAACTGCCCGACCCCGC
TTTTGCGCCGCTAACGATTTCCGCCATCGAGTTAATGGCGCAAAAGGTGCGAGAAAGTGATGAAGCTGTCACCCTTGTGC
CATCTGGCCCACTGACCAATATCGCGCTATTTATAGCGACCTACCCAGAGCTACATAGCAAGATTGAGCGTATCGTGCTG
ATGGGCGGCGCAGCAGAGGCCGGTAACTGGACACCGGCGGCTGAGTTCAATATTTTTGTTGACCCAGAAGCGGCTGATAT
GGTGTTTAAGTCAGGTATTCCTATCACCATGTGTGGCCTTGATATTACTCATCAAGCGCAGATCATGGACGAAGATATCG
AGCGTATTCGTGTTATCAACAACCCTGTCGCCAAGTGCGTGGCCGAGTTACTGGATTTCTTTATGATCTACCACAGAGAC
CCAAAGTGGGGCTTTGAAGGCGCGCCACTGCACGACCCTTGCACAATAGCTTGGTTACTTAAGCCTGAGCTATTTACCTC
GTACGATTGCTGGGTGGGTGTTGAAACAAAAGGCGAATACACCCAAGGGATGACAGTGGTTGACCGTTATCAGCTAACTA
ATAATCCACACAATACTCAAGTATTGTGCAAGCTTGATAGACAAGGCTTTGTCGACTTGATCGTTGAGTGTTTAGAGGCT
TATAACTAA

Upstream 100 bases:

>100_bases
AAGCGATTACTCAGTCACAGAATTTGCAGGCATTAGGCTCTAGGATGTTAAACAACCTACAAACCTATTTTAATAGACAC
ATATTTTGCGGAGCCAAACA

Downstream 100 bases:

>100_bases
CACTTAAAACTAACAATGTATCATGAGCTATTCATAGCGAATTGTTTCAATACATGGCTAACCGCTTCAATACAGGGTGA
AATAGTGTAACTATATTCAC

Product: ribonucleoside hydrolase 1

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSPTQAKQQSQKVTPIILDCDPGHDDAISLILAMSSKALKPLAVTTSAGNQTPDKTLNNALRILTLLERHDIPVAAGAPK
PLARELIIADNVHGESGLDGPKLPDPAFAPLTISAIELMAQKVRESDEAVTLVPSGPLTNIALFIATYPELHSKIERIVL
MGGAAEAGNWTPAAEFNIFVDPEAADMVFKSGIPITMCGLDITHQAQIMDEDIERIRVINNPVAKCVAELLDFFMIYHRD
PKWGFEGAPLHDPCTIAWLLKPELFTSYDCWVGVETKGEYTQGMTVVDRYQLTNNPHNTQVLCKLDRQGFVDLIVECLEA
YN

Sequences:

>Translated_322_residues
MSPTQAKQQSQKVTPIILDCDPGHDDAISLILAMSSKALKPLAVTTSAGNQTPDKTLNNALRILTLLERHDIPVAAGAPK
PLARELIIADNVHGESGLDGPKLPDPAFAPLTISAIELMAQKVRESDEAVTLVPSGPLTNIALFIATYPELHSKIERIVL
MGGAAEAGNWTPAAEFNIFVDPEAADMVFKSGIPITMCGLDITHQAQIMDEDIERIRVINNPVAKCVAELLDFFMIYHRD
PKWGFEGAPLHDPCTIAWLLKPELFTSYDCWVGVETKGEYTQGMTVVDRYQLTNNPHNTQVLCKLDRQGFVDLIVECLEA
YN
>Mature_321_residues
SPTQAKQQSQKVTPIILDCDPGHDDAISLILAMSSKALKPLAVTTSAGNQTPDKTLNNALRILTLLERHDIPVAAGAPKP
LARELIIADNVHGESGLDGPKLPDPAFAPLTISAIELMAQKVRESDEAVTLVPSGPLTNIALFIATYPELHSKIERIVLM
GGAAEAGNWTPAAEFNIFVDPEAADMVFKSGIPITMCGLDITHQAQIMDEDIERIRVINNPVAKCVAELLDFFMIYHRDP
KWGFEGAPLHDPCTIAWLLKPELFTSYDCWVGVETKGEYTQGMTVVDRYQLTNNPHNTQVLCKLDRQGFVDLIVECLEAY
N

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=307, Percent_Identity=69.0553745928339, Blast_Score=449, Evalue=1e-128,
Organism=Escherichia coli, GI1788486, Length=311, Percent_Identity=41.8006430868167, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1786213, Length=304, Percent_Identity=43.0921052631579, Blast_Score=209, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI32565474, Length=193, Percent_Identity=30.0518134715026, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17565698, Length=194, Percent_Identity=32.9896907216495, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6320608, Length=328, Percent_Identity=32.9268292682927, Blast_Score=138, Evalue=9e-34,
Organism=Drosophila melanogaster, GI24583915, Length=322, Percent_Identity=27.639751552795, Blast_Score=89, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24641839, Length=199, Percent_Identity=32.6633165829146, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24641837, Length=324, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 35267; Mature: 35136

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTQAKQQSQKVTPIILDCDPGHDDAISLILAMSSKALKPLAVTTSAGNQTPDKTLNNA
CCCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHH
LRILTLLERHDIPVAAGAPKPLARELIIADNVHGESGLDGPKLPDPAFAPLTISAIELMA
HHHHHHHHHCCCCEECCCCCHHHHHEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
QKVRESDEAVTLVPSGPLTNIALFIATYPELHSKIERIVLMGGAAEAGNWTPAAEFNIFV
HHHHCCCCEEEEECCCCHHHEEEEEEECHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEE
DPEAADMVFKSGIPITMCGLDITHQAQIMDEDIERIRVINNPVAKCVAELLDFFMIYHRD
CCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
PKWGFEGAPLHDPCTIAWLLKPELFTSYDCWVGVETKGEYTQGMTVVDRYQLTNNPHNTQ
CCCCCCCCCCCCCCEEEEEECCHHHCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCE
VLCKLDRQGFVDLIVECLEAYN
EEEEECCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
SPTQAKQQSQKVTPIILDCDPGHDDAISLILAMSSKALKPLAVTTSAGNQTPDKTLNNA
CCCHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHH
LRILTLLERHDIPVAAGAPKPLARELIIADNVHGESGLDGPKLPDPAFAPLTISAIELMA
HHHHHHHHHCCCCEECCCCCHHHHHEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
QKVRESDEAVTLVPSGPLTNIALFIATYPELHSKIERIVLMGGAAEAGNWTPAAEFNIFV
HHHHCCCCEEEEECCCCHHHEEEEEEECHHHHHHHHEEEEECCCCCCCCCCCCCEEEEEE
DPEAADMVFKSGIPITMCGLDITHQAQIMDEDIERIRVINNPVAKCVAELLDFFMIYHRD
CCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
PKWGFEGAPLHDPCTIAWLLKPELFTSYDCWVGVETKGEYTQGMTVVDRYQLTNNPHNTQ
CCCCCCCCCCCCCCEEEEEECCHHHCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCCE
VLCKLDRQGFVDLIVECLEAYN
EEEEECCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA