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The map label for this gene is pflA [H]

Identifier: 157961496

GI number: 157961496

Start: 2024862

End: 2025602

Strand: Reverse

Name: pflA [H]

Synonym: Spea_1671

Alternate gene names: 157961496

Gene position: 2025602-2024862 (Counterclockwise)

Preceding gene: 157961497

Following gene: 157961494

Centisome position: 39.15

GC content: 43.05

Gene sequence:

>741_bases
ATGGCAGTTATCGGTCGGATTCATTCAATAGAATCTTTTGGAACAGTAGATGGTCCTGGCATTCGGTTTATCACCTTTAT
GCAAGGCTGCTTAATGCGCTGCCTATACTGCCATAACCGCGACACTTGGGACTTACACGGTGGTAAAGAAATGAAAGTCG
ATGACTTAATGAGTCAAATCATTAGTTATCGACCTTTTCTTGAAGCCAGTGGCGGTGGTGTGACCGCAAGCGGCGGAGAA
GCGATTATCCAAGCTGATTTCGTAGCTGAACTTTTCAAAGCCTGTAAGAAAGAAGGTATCCATACCTGTCTCGATACCAA
CGGCTTTGTTCGTAAACACACACCGATTATCGATGAACTACTCGATAACACAGATTTGGTGATGCTCGATATTAAACAGA
TTGATGATGCAAAGCATATTGATCTCACTAATGTCAGTAACCAGCGTACGCTGCAGTTTGCAGAACACTTGCACAAGCGT
GGGCAAAAAGCATGGATTCGATATGTGGTTGTTGGTGGTTACACCGACGATATCCCATCAGCACAAGCCTTAGCTGAATT
TATTCAACCAATGAAAAACGTGGAAAAGGTCGAGTTACTCCCTTACCACGAGCTAGGCAAGCATAAATGGGAAGCCATGG
GTATGGATTATGAGCTTAATAATATCTCACCGCCAAGTACTGAAACCATGGAACAAATTAAGAAAGTCTTTACCGATATG
GGAATGAACGCGGTTTATTAA

Upstream 100 bases:

>100_bases
GACGTTATTACCCGTACCTTCACTAAAGGTCTGTAAGTTTAATTAAGTGTCGATTGAGCTGATGCTAGCTTAAGCAAGCA
TCAGCTAAACAGGAGTTTAA

Downstream 100 bases:

>100_bases
CCGCGTAAAATATTAGAACAATCAGCTAGAGCTCTTCCCAATAGCTGGCATCTAGGCGTTCGAAGGCCGCTTTTAGAATT
AGCGCCATATCGAAATAACA

Product: pyruvate formate lyase-activating enzyme 1

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 1; PFL-activating enzyme 1 [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MAVIGRIHSIESFGTVDGPGIRFITFMQGCLMRCLYCHNRDTWDLHGGKEMKVDDLMSQIISYRPFLEASGGGVTASGGE
AIIQADFVAELFKACKKEGIHTCLDTNGFVRKHTPIIDELLDNTDLVMLDIKQIDDAKHIDLTNVSNQRTLQFAEHLHKR
GQKAWIRYVVVGGYTDDIPSAQALAEFIQPMKNVEKVELLPYHELGKHKWEAMGMDYELNNISPPSTETMEQIKKVFTDM
GMNAVY

Sequences:

>Translated_246_residues
MAVIGRIHSIESFGTVDGPGIRFITFMQGCLMRCLYCHNRDTWDLHGGKEMKVDDLMSQIISYRPFLEASGGGVTASGGE
AIIQADFVAELFKACKKEGIHTCLDTNGFVRKHTPIIDELLDNTDLVMLDIKQIDDAKHIDLTNVSNQRTLQFAEHLHKR
GQKAWIRYVVVGGYTDDIPSAQALAEFIQPMKNVEKVELLPYHELGKHKWEAMGMDYELNNISPPSTETMEQIKKVFTDM
GMNAVY
>Mature_245_residues
AVIGRIHSIESFGTVDGPGIRFITFMQGCLMRCLYCHNRDTWDLHGGKEMKVDDLMSQIISYRPFLEASGGGVTASGGEA
IIQADFVAELFKACKKEGIHTCLDTNGFVRKHTPIIDELLDNTDLVMLDIKQIDDAKHIDLTNVSNQRTLQFAEHLHKRG
QKAWIRYVVVGGYTDDIPSAQALAEFIQPMKNVEKVELLPYHELGKHKWEAMGMDYELNNISPPSTETMEQIKKVFTDMG
MNAVY

Specific function: Activation of pyruvate formate-lyase 1 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the organic radical-activating enzymes family [H]

Homologues:

Organism=Escherichia coli, GI1787130, Length=246, Percent_Identity=66.260162601626, Blast_Score=358, Evalue=1e-100,
Organism=Escherichia coli, GI1790389, Length=270, Percent_Identity=23.7037037037037, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790839, Length=249, Percent_Identity=30.1204819277108, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI226510931, Length=299, Percent_Identity=24.7491638795987, Blast_Score=75, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR012838
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 27725; Mature: 27594

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: PS01087 RADICAL_ACTIVATING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIGRIHSIESFGTVDGPGIRFITFMQGCLMRCLYCHNRDTWDLHGGKEMKVDDLMSQI
CCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHH
ISYRPFLEASGGGVTASGGEAIIQADFVAELFKACKKEGIHTCLDTNGFVRKHTPIIDEL
HHHCCCEECCCCCEEECCCCCEEHHHHHHHHHHHHHHCCCCCHHCCCCCEECCCCHHHHH
LDNTDLVMLDIKQIDDAKHIDLTNVSNQRTLQFAEHLHKRGQKAWIRYVVVGGYTDDIPS
HCCCCEEEEEHHHCCCCCCEEECCCCCHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCC
AQALAEFIQPMKNVEKVELLPYHELGKHKWEAMGMDYELNNISPPSTETMEQIKKVFTDM
HHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHC
GMNAVY
CCCCCC
>Mature Secondary Structure 
AVIGRIHSIESFGTVDGPGIRFITFMQGCLMRCLYCHNRDTWDLHGGKEMKVDDLMSQI
CCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHHHH
ISYRPFLEASGGGVTASGGEAIIQADFVAELFKACKKEGIHTCLDTNGFVRKHTPIIDEL
HHHCCCEECCCCCEEECCCCCEEHHHHHHHHHHHHHHCCCCCHHCCCCCEECCCCHHHHH
LDNTDLVMLDIKQIDDAKHIDLTNVSNQRTLQFAEHLHKRGQKAWIRYVVVGGYTDDIPS
HCCCCEEEEEHHHCCCCCCEEECCCCCHHHHHHHHHHHHCCHHEEEEEEEECCCCCCCCC
AQALAEFIQPMKNVEKVELLPYHELGKHKWEAMGMDYELNNISPPSTETMEQIKKVFTDM
HHHHHHHHHHHHCCHHEEECCHHHHHHHHHHHCCCCEECCCCCCCCHHHHHHHHHHHHHC
GMNAVY
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]