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The map label for this gene is ackA

Identifier: 157961494

GI number: 157961494

Start: 2023001

End: 2024197

Strand: Reverse

Name: ackA

Synonym: Spea_1669

Alternate gene names: 157961494

Gene position: 2024197-2023001 (Counterclockwise)

Preceding gene: 157961496

Following gene: 157961493

Centisome position: 39.12

GC content: 45.78

Gene sequence:

>1197_bases
ATGTCACACAAACTAGTATTGGTGCTTAACTGTGGTAGTTCATCTCTAAAGTTTGCCGTTATTGATGCGCTTACTGGCGA
TGACCAAATTTCAGGTCTAGCTGAGTGTTTTGGTCTAGAAGATTCTAGAATCAAGTGGAAAGTTAACGGCCAAAAGAGTG
AAGCGAGCCTAGGTGCTTTCACTGCTCACAGAGAAGCAGTTGAATACATTGTTAACGACATTCTAGGTGCTCACCCAGAA
ATCGCAGCAGAGATCCAAGCAATTGGTCACCGTGTTGTACACGGCGGTGAGAAGTTCACTCGCTCAGTAATTATTGACGA
ATCAGTTATTCACGGTATTGAAGATTGTGCAACTTTAGCACCTCTTCACAACCCAGCTCACCTAATCGGTATTCGTGCTG
CGCAAGCTTCATTCCCTGCCCTACCACAAGTAGCCGTGTTTGATACTGCGTTCCACCAGACTATGCCAGAAAAAGCATAC
ATCTACGCACTTCCATACAAGCTATACCGCGAAAACGCGATCCGTCGCTATGGCATGCACGGTACTAGCCACCTATTCAT
CAGCCGCGAAGCAGCTGCTGCACTAGGTAAAGACGAAGCTGACACAAACATCATTTGTGCTCACCTAGGTAACGGCGCAT
CAGTTACTGCTATTAAAGGCGGTAAGAGTGTTGATACTTCTATGGGTCTAACTCCACTAGAAGGTTTGGTAATGGGTACA
CGTTGTGGTGACCTTGACCCTTCAGTTATCTTCCACCTAGTTAACCGTTTAGGTTACACACTAGATGAAGTTGAATCTGT
ACTGAACAAGCAAAGTGGTCTGCTAGGTATTTCTGAGCTTACTAACGATTGCCGTGGCATCGAAGAAGGTTTCGGAAGTG
GTCATAAAGGCGCTACGCTAGCACTAGAAATCTTCTGCTACCGTCTAGCTAAGTACATCGCTTCTTACACTGTACCTTTA
GAGCGTCTTGACGCTGTAGTGTTCACTGGCGGTATCGGTGAAAACTCTGACCTAATCCGTGAGAAAGTACTTAACTCTCT
AGCGATCTTCAACTTCAACGTTGATAAAGAGCGTAACGCTGCAGCTCGTTTCGGTAACGGTGGCCAGATCACTACCGACG
AAGGTACTGTTGCTATGGTTATCCCTACTAATGAAGAGTGGGTTATTGCGCAAGACGCAATCGAGCTAATCAAGTAA

Upstream 100 bases:

>100_bases
TTAAAAGAGCATACTTTACGACTTCGGCTATAGTTGCCATATGAAGTTTTGTTATTCGGTGAGTCAGACGACTTCACCAT
TTATTGCGAAAGGTTTAAAC

Downstream 100 bases:

>100_bases
TAATGCAAGAAAATGCAGCTAATACTCAGTATTAGCTGCAGTTAGCGCAGTAAAGATTTTAGTCATACTTTGTATGGCAT
CACCGAGTTTCAAGAGGTCA

Product: acetate kinase

Products: NA

Alternate protein names: Acetokinase

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSHKLVLVLNCGSSSLKFAVIDALTGDDQISGLAECFGLEDSRIKWKVNGQKSEASLGAFTAHREAVEYIVNDILGAHPE
IAAEIQAIGHRVVHGGEKFTRSVIIDESVIHGIEDCATLAPLHNPAHLIGIRAAQASFPALPQVAVFDTAFHQTMPEKAY
IYALPYKLYRENAIRRYGMHGTSHLFISREAAAALGKDEADTNIICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGT
RCGDLDPSVIFHLVNRLGYTLDEVESVLNKQSGLLGISELTNDCRGIEEGFGSGHKGATLALEIFCYRLAKYIASYTVPL
ERLDAVVFTGGIGENSDLIREKVLNSLAIFNFNVDKERNAAARFGNGGQITTDEGTVAMVIPTNEEWVIAQDAIELIK

Sequences:

>Translated_398_residues
MSHKLVLVLNCGSSSLKFAVIDALTGDDQISGLAECFGLEDSRIKWKVNGQKSEASLGAFTAHREAVEYIVNDILGAHPE
IAAEIQAIGHRVVHGGEKFTRSVIIDESVIHGIEDCATLAPLHNPAHLIGIRAAQASFPALPQVAVFDTAFHQTMPEKAY
IYALPYKLYRENAIRRYGMHGTSHLFISREAAAALGKDEADTNIICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGT
RCGDLDPSVIFHLVNRLGYTLDEVESVLNKQSGLLGISELTNDCRGIEEGFGSGHKGATLALEIFCYRLAKYIASYTVPL
ERLDAVVFTGGIGENSDLIREKVLNSLAIFNFNVDKERNAAARFGNGGQITTDEGTVAMVIPTNEEWVIAQDAIELIK
>Mature_397_residues
SHKLVLVLNCGSSSLKFAVIDALTGDDQISGLAECFGLEDSRIKWKVNGQKSEASLGAFTAHREAVEYIVNDILGAHPEI
AAEIQAIGHRVVHGGEKFTRSVIIDESVIHGIEDCATLAPLHNPAHLIGIRAAQASFPALPQVAVFDTAFHQTMPEKAYI
YALPYKLYRENAIRRYGMHGTSHLFISREAAAALGKDEADTNIICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGTR
CGDLDPSVIFHLVNRLGYTLDEVESVLNKQSGLLGISELTNDCRGIEEGFGSGHKGATLALEIFCYRLAKYIASYTVPLE
RLDAVVFTGGIGENSDLIREKVLNSLAIFNFNVDKERNAAARFGNGGQITTDEGTVAMVIPTNEEWVIAQDAIELIK

Specific function: Involved In The Activation Of Acetate To Acetyl CoA And The Secretion Of Acetate. During Anaerobic Growth Of The Organism, This Enzyme Is Also Involved In The Synthesis Of Most Of The ATP Formed Catabolically. [C]

COG id: COG0282

COG function: function code C; Acetate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family

Homologues:

Organism=Escherichia coli, GI1788633, Length=399, Percent_Identity=61.6541353383459, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI145693184, Length=396, Percent_Identity=46.2121212121212, Blast_Score=339, Evalue=2e-94,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): ACKA_SHEPA (A8H356)

Other databases:

- EMBL:   CP000851
- RefSeq:   YP_001501528.1
- ProteinModelPortal:   A8H356
- SMR:   A8H356
- STRING:   A8H356
- GeneID:   5662065
- GenomeReviews:   CP000851_GR
- KEGG:   spl:Spea_1669
- NMPDR:   fig|398579.3.peg.1438
- eggNOG:   COG0282
- HOGENOM:   HBG285863
- OMA:   ERMINHA
- ProtClustDB:   PRK00180
- BioCyc:   SPEA398579:SPEA_1669-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00020
- InterPro:   IPR000890
- InterPro:   IPR004372
- PANTHER:   PTHR21060
- PANTHER:   PTHR21060:SF11
- PIRSF:   PIRSF000722
- PRINTS:   PR00471
- TIGRFAMs:   TIGR00016

Pfam domain/function: PF00871 Acetate_kinase

EC number: =2.7.2.1

Molecular weight: Translated: 42849; Mature: 42718

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS01075 ACETATE_KINASE_1; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHKLVLVLNCGSSSLKFAVIDALTGDDQISGLAECFGLEDSRIKWKVNGQKSEASLGAF
CCCEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHH
TAHREAVEYIVNDILGAHPEIAAEIQAIGHRVVHGGEKFTRSVIIDESVIHGIEDCATLA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHEECHHHHHHHHHHHHHC
PLHNPAHLIGIRAAQASFPALPQVAVFDTAFHQTMPEKAYIYALPYKLYRENAIRRYGMH
CCCCCHHEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCC
GTSHLFISREAAAALGKDEADTNIICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGT
CCEEEEEEHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEEC
RCGDLDPSVIFHLVNRLGYTLDEVESVLNKQSGLLGISELTNDCRGIEEGFGSGHKGATL
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCHHH
ALEIFCYRLAKYIASYTVPLERLDAVVFTGGIGENSDLIREKVLNSLAIFNFNVDKERNA
HHHHHHHHHHHHHHHHCCCHHHHCEEEEECCCCCCHHHHHHHHHHHHEEEEECCCCCCCH
AARFGNGGQITTDEGTVAMVIPTNEEWVIAQDAIELIK
HHHCCCCCEEEECCCEEEEEEECCCCEEEHHHHHHHHC
>Mature Secondary Structure 
SHKLVLVLNCGSSSLKFAVIDALTGDDQISGLAECFGLEDSRIKWKVNGQKSEASLGAF
CCEEEEEEECCCCCCEEEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHH
TAHREAVEYIVNDILGAHPEIAAEIQAIGHRVVHGGEKFTRSVIIDESVIHGIEDCATLA
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHEECHHHHHHHHHHHHHC
PLHNPAHLIGIRAAQASFPALPQVAVFDTAFHQTMPEKAYIYALPYKLYRENAIRRYGMH
CCCCCHHEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCC
GTSHLFISREAAAALGKDEADTNIICAHLGNGASVTAIKGGKSVDTSMGLTPLEGLVMGT
CCEEEEEEHHHHHHCCCCCCCCCEEEEECCCCCEEEEECCCCCCCCCCCCCCHHHEEEEC
RCGDLDPSVIFHLVNRLGYTLDEVESVLNKQSGLLGISELTNDCRGIEEGFGSGHKGATL
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHCCCCCCCHHH
ALEIFCYRLAKYIASYTVPLERLDAVVFTGGIGENSDLIREKVLNSLAIFNFNVDKERNA
HHHHHHHHHHHHHHHHCCCHHHHCEEEEECCCCCCHHHHHHHHHHHHEEEEECCCCCCCH
AARFGNGGQITTDEGTVAMVIPTNEEWVIAQDAIELIK
HHHCCCCCEEEECCCEEEEEEECCCCEEEHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA