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The map label for this gene is pflB [H]

Identifier: 157961497

GI number: 157961497

Start: 2025667

End: 2027949

Strand: Reverse

Name: pflB [H]

Synonym: Spea_1672

Alternate gene names: 157961497

Gene position: 2027949-2025667 (Counterclockwise)

Preceding gene: 157961498

Following gene: 157961496

Centisome position: 39.19

GC content: 45.86

Gene sequence:

>2283_bases
ATGACCGAAAAAACTGAACAGTTTGCAACCGCGTGGGAAGGTTTCACCGCTGGCGATTGGAAAACTGAAGTCAATGTGCG
TGACTTTATTCAAGCTAACTACACTCCTTATGAAGGCGACGAGTCTTTCCTTGCTGGTGCTACTGAAGCAACAACCGAAC
TTTGGGACAAAGTTATGGTTGGCGTTAAGGAAGAGAACCGCACTCACGCTCCTGTAGATTTTGATACATCTCGCGTATCA
ACAATCACTTCTCACGAAGCTGGTTACATTACTAAAGATCTAGAAACTATCGTTGGTCTGCAAACAGAAGCTCCGCTTAA
GCGCGCTATGTTGCCAAACGGTGGTATTCGTATGGTTGAAAGTTCTTGCGCTGCATACGGCCGTGAACTAGACGCTGACG
TAAAATACGTATACTCAGAGCTACGTAAGACACACAACCAAGGTGTGTTTGATATCTACACTCCAGAAATCATGCGTTGT
CGTAAGTCTGGCGTATTGACTGGTTTACCAGATGCATACGGCCGTGGTCGTATCATTGGTGACTACCGTCGTGTAGCGCT
ATACGGTATTGATTTCCTAATGGCGGACAAGTTTGCTCAGTTCACTTCTCTTCAGTCTGATTTCGAAACTGGCGAAAACT
TGTCTTACACTATGCAACTTCGTGAAGAAATTGCTGAGCAGCACAAAGCACTAGGTCAAATGAAGAAGATGGCAGCAAGC
TACGGCTTCGACATCTCTCTACCTGCGACTAGCGCTCAAGAAGCTATCCAGTGGACTTACTTCGGCTACCTAGCTGCAGT
TAAGAGCCAGAACGGCGCGGCTATGTCTTTAGGTCGTACTTCTAGCTTCCTAGATATCTTCATTGAACGCGACATCAAGA
ATGGCGTGATCACTGAGCAACAAGCTCAGGAAATGGTTGACCATTTCGTAATGAAGCTACGTATGGTTCGTTTCCTACGT
ACTCCAGAATACGATGAGTTATTCTCTGGCGACCCAATCTGGGCTACTGAGTCTATCGCTGGTATGGGTCTAGACGGCCG
TACATTGGTTACTAAGTCTAGCTTCCGTTTCCTACACACATTGTACAACATGGGTCCTAGCCCAGAGCCAAACATCACGG
TTCTATGGTCAGAGCAACTACCGCTAAACTTCAAGAAGTACGCGTCTAAAGTATCTATCGATACTAGCTCTATCCAGTAC
GAAAACGATGACCTAATGCGTCCAGATTTCGAATCTGACGATTACGCTATCGCTTGTTGTGTAAGCCCAATGGTTGTTGG
TAAACACATGCAGTTCTTCGGCGCGCGTGCAAACCTTGCCAAGACTATGCTTTACGCTATCAACGGCGGTGTTGACGAAA
AGCTTAACGCTCAAATCGGTCCTAAGTCTGCGCCAATCACAGACGAAGTATTGAACTATGATGACGTTATGGGTCGTTTA
GACACTATGATGGACTGGTTAGCTACACAATATGTGTCTGCACTAAACGCTATCCACTTCATGCACGACAAGTACTCTTA
TGAATCAGCACTAATGGCTCTTCATGACAGAGACGTACGTCGTACTATGGCTTGTGGTATCGCAGGTCTTTCTATCACTG
CTGACTCACTAGCAGCGATTAAGTACGCAACTGTTAAGCCAATCCGTGATGAAAACGGTATTGCTGTAGACTTCGACATC
GAAGGCGACTATCCAAAGTTCGGTAACAACGACTCACGTGTTGATGATATCGCTACTGAATTGGTTGAGCGCTTCATGGC
GAAGATCCGTAACATGAAGATGTACCGTAACGCGATCCCAACTCAGTCTATCTTAACCATTACCTCTAACGTGGTTTACG
GTAAGAAGACAGGTAACACACCTGACGGTCGCCGCTCTGGTGCACCATTTGCTCCAGGTGCTAACCCAATGCACGGTCGT
GATGAAAAAGGCGCTATCGCTTCTTTAACATCTGTAGCTAAACTACCATTTGCTCACGCTCAAGACGGTATTTCTTACAC
ATTCTCAATCGTACCTAACGCACTAGGTAAAGATGATGATGCACGTAAGACAAACCTTGCAGCGCTTATGGATGGTTACT
TCGCTCACAACGAGAACCGTGAAGGTGGTCAACACTTAAACGTTAACGTTATGAACCGCGAAATGCTTGAAGATGCAGTG
GTTAATCCAGATATGTATCCACAGCTAACTATCCGTGTTTCTGGCTACGCAGTACGCTTTAACTCTCTAACACCAGAACA
GCAGCAAGACGTTATTACCCGTACCTTCACTAAAGGTCTGTAA

Upstream 100 bases:

>100_bases
GTTGGGGCGTTTAAGACTTAACTATTGGTGTATCGCACGCCTCGTTTTGAGCCTATATCGATACCCAATAACCTTATCTC
AAACAGAAGGTAATAGTACG

Downstream 100 bases:

>100_bases
GTTTAATTAAGTGTCGATTGAGCTGATGCTAGCTTAAGCAAGCATCAGCTAAACAGGAGTTTAAATGGCAGTTATCGGTC
GGATTCATTCAATAGAATCT

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 1 [H]

Number of amino acids: Translated: 760; Mature: 759

Protein sequence:

>760_residues
MTEKTEQFATAWEGFTAGDWKTEVNVRDFIQANYTPYEGDESFLAGATEATTELWDKVMVGVKEENRTHAPVDFDTSRVS
TITSHEAGYITKDLETIVGLQTEAPLKRAMLPNGGIRMVESSCAAYGRELDADVKYVYSELRKTHNQGVFDIYTPEIMRC
RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMADKFAQFTSLQSDFETGENLSYTMQLREEIAEQHKALGQMKKMAAS
YGFDISLPATSAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIFIERDIKNGVITEQQAQEMVDHFVMKLRMVRFLR
TPEYDELFSGDPIWATESIAGMGLDGRTLVTKSSFRFLHTLYNMGPSPEPNITVLWSEQLPLNFKKYASKVSIDTSSIQY
ENDDLMRPDFESDDYAIACCVSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLNAQIGPKSAPITDEVLNYDDVMGRL
DTMMDWLATQYVSALNAIHFMHDKYSYESALMALHDRDVRRTMACGIAGLSITADSLAAIKYATVKPIRDENGIAVDFDI
EGDYPKFGNNDSRVDDIATELVERFMAKIRNMKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGR
DEKGAIASLTSVAKLPFAHAQDGISYTFSIVPNALGKDDDARKTNLAALMDGYFAHNENREGGQHLNVNVMNREMLEDAV
VNPDMYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL

Sequences:

>Translated_760_residues
MTEKTEQFATAWEGFTAGDWKTEVNVRDFIQANYTPYEGDESFLAGATEATTELWDKVMVGVKEENRTHAPVDFDTSRVS
TITSHEAGYITKDLETIVGLQTEAPLKRAMLPNGGIRMVESSCAAYGRELDADVKYVYSELRKTHNQGVFDIYTPEIMRC
RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMADKFAQFTSLQSDFETGENLSYTMQLREEIAEQHKALGQMKKMAAS
YGFDISLPATSAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIFIERDIKNGVITEQQAQEMVDHFVMKLRMVRFLR
TPEYDELFSGDPIWATESIAGMGLDGRTLVTKSSFRFLHTLYNMGPSPEPNITVLWSEQLPLNFKKYASKVSIDTSSIQY
ENDDLMRPDFESDDYAIACCVSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLNAQIGPKSAPITDEVLNYDDVMGRL
DTMMDWLATQYVSALNAIHFMHDKYSYESALMALHDRDVRRTMACGIAGLSITADSLAAIKYATVKPIRDENGIAVDFDI
EGDYPKFGNNDSRVDDIATELVERFMAKIRNMKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGR
DEKGAIASLTSVAKLPFAHAQDGISYTFSIVPNALGKDDDARKTNLAALMDGYFAHNENREGGQHLNVNVMNREMLEDAV
VNPDMYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL
>Mature_759_residues
TEKTEQFATAWEGFTAGDWKTEVNVRDFIQANYTPYEGDESFLAGATEATTELWDKVMVGVKEENRTHAPVDFDTSRVST
ITSHEAGYITKDLETIVGLQTEAPLKRAMLPNGGIRMVESSCAAYGRELDADVKYVYSELRKTHNQGVFDIYTPEIMRCR
KSGVLTGLPDAYGRGRIIGDYRRVALYGIDFLMADKFAQFTSLQSDFETGENLSYTMQLREEIAEQHKALGQMKKMAASY
GFDISLPATSAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIFIERDIKNGVITEQQAQEMVDHFVMKLRMVRFLRT
PEYDELFSGDPIWATESIAGMGLDGRTLVTKSSFRFLHTLYNMGPSPEPNITVLWSEQLPLNFKKYASKVSIDTSSIQYE
NDDLMRPDFESDDYAIACCVSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLNAQIGPKSAPITDEVLNYDDVMGRLD
TMMDWLATQYVSALNAIHFMHDKYSYESALMALHDRDVRRTMACGIAGLSITADSLAAIKYATVKPIRDENGIAVDFDIE
GDYPKFGNNDSRVDDIATELVERFMAKIRNMKMYRNAIPTQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGRD
EKGAIASLTSVAKLPFAHAQDGISYTFSIVPNALGKDDDARKTNLAALMDGYFAHNENREGGQHLNVNVMNREMLEDAVV
NPDMYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1787131, Length=760, Percent_Identity=81.1842105263158, Blast_Score=1331, Evalue=0.0,
Organism=Escherichia coli, GI48994926, Length=753, Percent_Identity=74.5019920318725, Blast_Score=1219, Evalue=0.0,
Organism=Escherichia coli, GI1787044, Length=583, Percent_Identity=27.1012006861063, Blast_Score=175, Evalue=8e-45,
Organism=Escherichia coli, GI1790388, Length=642, Percent_Identity=24.9221183800623, Blast_Score=130, Evalue=4e-31,
Organism=Escherichia coli, GI1788933, Length=60, Percent_Identity=81.6666666666667, Blast_Score=107, Evalue=3e-24,

Paralogues:

None

Copy number: 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005949
- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 84767; Mature: 84636

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEKTEQFATAWEGFTAGDWKTEVNVRDFIQANYTPYEGDESFLAGATEATTELWDKVMV
CCCHHHHHHHHHCCCCCCCCEECCCHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHH
GVKEENRTHAPVDFDTSRVSTITSHEAGYITKDLETIVGLQTEAPLKRAMLPNGGIRMVE
CCCCCCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHH
SSCAAYGRELDADVKYVYSELRKTHNQGVFDIYTPEIMRCRKSGVLTGLPDAYGRGRIIG
HHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCEEH
DYRRVALYGIDFLMADKFAQFTSLQSDFETGENLSYTMQLREEIAEQHKALGQMKKMAAS
HHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YGFDISLPATSAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIFIERDIKNGVITEQ
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCHH
QAQEMVDHFVMKLRMVRFLRTPEYDELFSGDPIWATESIAGMGLDGRTLVTKSSFRFLHT
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEECCCHHCCCCCCCEEEEHHHHHHHHH
LYNMGPSPEPNITVLWSEQLPLNFKKYASKVSIDTSSIQYENDDLMRPDFESDDYAIACC
HHHCCCCCCCCEEEEECCCCCCCHHHHHHHEEECCCCEEECCCCCCCCCCCCCCCEEEEE
VSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLNAQIGPKSAPITDEVLNYDDVMGRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHH
DTMMDWLATQYVSALNAIHFMHDKYSYESALMALHDRDVRRTMACGIAGLSITADSLAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHE
KYATVKPIRDENGIAVDFDIEGDYPKFGNNDSRVDDIATELVERFMAKIRNMKMYRNAIP
EEEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGRDEKGAIASLTSVAKLPFAHA
CHHHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHC
QDGISYTFSIVPNALGKDDDARKTNLAALMDGYFAHNENREGGQHLNVNVMNREMLEDAV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHH
VNPDMYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL
CCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEKTEQFATAWEGFTAGDWKTEVNVRDFIQANYTPYEGDESFLAGATEATTELWDKVMV
CCHHHHHHHHHCCCCCCCCEECCCHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHH
GVKEENRTHAPVDFDTSRVSTITSHEAGYITKDLETIVGLQTEAPLKRAMLPNGGIRMVE
CCCCCCCCCCCCCCCCCHHHEECCCCCCCHHHHHHHHHCCCCCCCHHHHCCCCCCHHHHH
SSCAAYGRELDADVKYVYSELRKTHNQGVFDIYTPEIMRCRKSGVLTGLPDAYGRGRIIG
HHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCCCCCCCCEEH
DYRRVALYGIDFLMADKFAQFTSLQSDFETGENLSYTMQLREEIAEQHKALGQMKKMAAS
HHHEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YGFDISLPATSAQEAIQWTYFGYLAAVKSQNGAAMSLGRTSSFLDIFIERDIKNGVITEQ
CCCEEECCCCCHHHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHCCCCCCCHH
QAQEMVDHFVMKLRMVRFLRTPEYDELFSGDPIWATESIAGMGLDGRTLVTKSSFRFLHT
HHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCEECCCHHCCCCCCCEEEEHHHHHHHHH
LYNMGPSPEPNITVLWSEQLPLNFKKYASKVSIDTSSIQYENDDLMRPDFESDDYAIACC
HHHCCCCCCCCEEEEECCCCCCCHHHHHHHEEECCCCEEECCCCCCCCCCCCCCCEEEEE
VSPMVVGKHMQFFGARANLAKTMLYAINGGVDEKLNAQIGPKSAPITDEVLNYDDVMGRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCHHHHHHHH
DTMMDWLATQYVSALNAIHFMHDKYSYESALMALHDRDVRRTMACGIAGLSITADSLAAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECHHHHHHE
KYATVKPIRDENGIAVDFDIEGDYPKFGNNDSRVDDIATELVERFMAKIRNMKMYRNAIP
EEEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TQSILTITSNVVYGKKTGNTPDGRRSGAPFAPGANPMHGRDEKGAIASLTSVAKLPFAHA
CHHHHEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHC
QDGISYTFSIVPNALGKDDDARKTNLAALMDGYFAHNENREGGQHLNVNVMNREMLEDAV
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHHH
VNPDMYPQLTIRVSGYAVRFNSLTPEQQQDVITRTFTKGL
CCCCCCCEEEEEEEEEEEEECCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3053170; 8905232; 9278503; 2651404; 1310545; 9629924; 10504733 [H]