Click here to switch to the map view.

The map label for this gene is rluB [H]

Identifier: 157961474

GI number: 157961474

Start: 1991861

End: 1992733

Strand: Direct

Name: rluB [H]

Synonym: Spea_1648

Alternate gene names: 157961474

Gene position: 1991861-1992733 (Clockwise)

Preceding gene: 157961473

Following gene: 157961476

Centisome position: 38.49

GC content: 47.08

Gene sequence:

>873_bases
ATGAGTGAAAAATTGCAGAAAGTCTTGGCCCGTGCAGGCCATGGCTCCCGTCGTGAGATGGAGGCATGGATTGCTGCAGG
TCGAGTTAGTGTTGACGGCGAAATTGCAAGCCTAGGTGATCGCGTTGAATCTGACGCAAAGATCCGTATCGATGGCCGTA
CAGTTTCAATTCGTTCTGAAGAAGATGTGGTTTGTCGTGTTATTGCATACCACAAACCTGAAGGTGAAATCTGTAGTCGT
AAAGACCCAGAAGGACGCCCAACGGTATTTGACCGTCTACCAAAAACTCGCGATTCACGCTGGGTTGCTGTAGGACGCTT
GGATATCAACACATCAGGTTTATTGTTGTTTACCTCTGATGGTGAACTAGCAAACCGCCTAATGCACCCATCAAATGAAG
TGGAGCGTGAATACGCTGTACGTACGTTTGGTGAAGTGAATGACGCTTGTATCCAACGTTTACGCATGGGCGTAACGTTA
GAAGATGGCCCAGCAAACTTTGACAAAGTTAAAGCTGCTGGTGGCGAAGGCATGAACAAGTGGTGGCATGTCAGCTTAGC
TGAAGGCCGTAACCGTGAAGTTCGTCGTCTATGGGAATCTCAAGAGGTTCAGGTAAGTCGTCTTATCCGTATTCGTTACG
GTATGATTGAACTGCCTAAAGCTCTGCCACGTGGTGGTTGGATTGAGTTGCCAATTGAAGAAGTTAACTACTTACGTAAA
ACAGCGGGTATGGAAGAGGAAACTCGTACATTGCTGGGCACTGATAAACACAGTGTTGCACGTAATAAAGTACGTAGCGC
AAAAATCAAGCGCGCTGTACATAAGCATAAAGTTGCTACAGGTAAGCCAAAGCCAACGCGTCAGCGTAGCTAA

Upstream 100 bases:

>100_bases
GACAGTATTGCGGATCTACCGCCTCTGTCAGATGCCAAATCATTGGACGCGCTGTTCCATGTTGAGAGCGGCGTGACCGA
AAAAACAGAAGAGTCTACTA

Downstream 100 bases:

>100_bases
TTGCTAACCAGCCTATCGACTAGAGATAGTCGAACAAAACGCATTTAAAAGGCCGCTATTTTAGCGGCCTTTTTGTATAT
AATGTCTTGTCCTGAAATGT

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSEKLQKVLARAGHGSRREMEAWIAAGRVSVDGEIASLGDRVESDAKIRIDGRTVSIRSEEDVVCRVIAYHKPEGEICSR
KDPEGRPTVFDRLPKTRDSRWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVEREYAVRTFGEVNDACIQRLRMGVTL
EDGPANFDKVKAAGGEGMNKWWHVSLAEGRNREVRRLWESQEVQVSRLIRIRYGMIELPKALPRGGWIELPIEEVNYLRK
TAGMEEETRTLLGTDKHSVARNKVRSAKIKRAVHKHKVATGKPKPTRQRS

Sequences:

>Translated_290_residues
MSEKLQKVLARAGHGSRREMEAWIAAGRVSVDGEIASLGDRVESDAKIRIDGRTVSIRSEEDVVCRVIAYHKPEGEICSR
KDPEGRPTVFDRLPKTRDSRWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVEREYAVRTFGEVNDACIQRLRMGVTL
EDGPANFDKVKAAGGEGMNKWWHVSLAEGRNREVRRLWESQEVQVSRLIRIRYGMIELPKALPRGGWIELPIEEVNYLRK
TAGMEEETRTLLGTDKHSVARNKVRSAKIKRAVHKHKVATGKPKPTRQRS
>Mature_289_residues
SEKLQKVLARAGHGSRREMEAWIAAGRVSVDGEIASLGDRVESDAKIRIDGRTVSIRSEEDVVCRVIAYHKPEGEICSRK
DPEGRPTVFDRLPKTRDSRWVAVGRLDINTSGLLLFTSDGELANRLMHPSNEVEREYAVRTFGEVNDACIQRLRMGVTLE
DGPANFDKVKAAGGEGMNKWWHVSLAEGRNREVRRLWESQEVQVSRLIRIRYGMIELPKALPRGGWIELPIEEVNYLRKT
AGMEEETRTLLGTDKHSVARNKVRSAKIKRAVHKHKVATGKPKPTRQRS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=283, Percent_Identity=64.3109540636042, Blast_Score=373, Evalue=1e-105,
Organism=Escherichia coli, GI1790453, Length=239, Percent_Identity=28.8702928870293, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=30.3418803418803, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=31.6062176165803, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 32796; Mature: 32665

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEKLQKVLARAGHGSRREMEAWIAAGRVSVDGEIASLGDRVESDAKIRIDGRTVSIRSE
CCHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCHHHHHHHHCCCCCEEEECCEEEEEECC
EDVVCRVIAYHKPEGEICSRKDPEGRPTVFDRLPKTRDSRWVAVGRLDINTSGLLLFTSD
CCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEEECCCEEEEEECC
GELANRLMHPSNEVEREYAVRTFGEVNDACIQRLRMGVTLEDGPANFDKVKAAGGEGMNK
CHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCEECCCCCCHHHHHCCCCCCCCC
WWHVSLAEGRNREVRRLWESQEVQVSRLIRIRYGMIELPKALPRGGWIELPIEEVNYLRK
EEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHCCCCCEEEECHHHHHHHHH
TAGMEEETRTLLGTDKHSVARNKVRSAKIKRAVHKHKVATGKPKPTRQRS
HCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SEKLQKVLARAGHGSRREMEAWIAAGRVSVDGEIASLGDRVESDAKIRIDGRTVSIRSE
CHHHHHHHHHCCCCCHHHHHHHHHHCCEEECCHHHHHHHHCCCCCEEEECCEEEEEECC
EDVVCRVIAYHKPEGEICSRKDPEGRPTVFDRLPKTRDSRWVAVGRLDINTSGLLLFTSD
CCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEEECCCEEEEEECC
GELANRLMHPSNEVEREYAVRTFGEVNDACIQRLRMGVTLEDGPANFDKVKAAGGEGMNK
CHHHHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCEECCCCCCHHHHHCCCCCCCCC
WWHVSLAEGRNREVRRLWESQEVQVSRLIRIRYGMIELPKALPRGGWIELPIEEVNYLRK
EEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHCCCCCEEEECHHHHHHHHH
TAGMEEETRTLLGTDKHSVARNKVRSAKIKRAVHKHKVATGKPKPTRQRS
HCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]