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The map label for this gene is ogt [H]

Identifier: 157961285

GI number: 157961285

Start: 1781045

End: 1781608

Strand: Direct

Name: ogt [H]

Synonym: Spea_1459

Alternate gene names: 157961285

Gene position: 1781045-1781608 (Clockwise)

Preceding gene: 157961284

Following gene: 157961286

Centisome position: 34.42

GC content: 43.79

Gene sequence:

>564_bases
ATGAATAAACAATCACAACCTTTGGTGAAACTCAATTTTGAATCAGATTATGGAGTGATTTCCTTATGCGCAAATGAAAC
AGGGATCACTCATTTAAATTTTATTCCTCAAATTGATAGTGAATGGCAGTCTAGGGAAGTTGATAACCAGTTGCTTGAAA
ATAATCGTGTCCAAAGCTCGATTGGCTCAGTGAGTGAGCCGGCATTAGAGCAAAGAGCAAGTGCGCATCTATTACAGGCA
AAACAGGAGCTTTGTGAGTATTTTTCCGGTACGCGACGTCACTTTGATGTAGCCTTAGCGCCTAAGGGCACCGAGTTTCA
ACAGCAAGTATGGCACGCGTTAACAGAGCTAGATTATGGGCAAAGTTGCAGCTACGCCGATATCGCGATGAAAATCGCAA
GGCCAAAAGCTGTGCGCGCAGTGGGTACGGCAAACGGCGCTAATCCTATTGCGATTATTGTGCCTTGCCATAGAGTGATA
GGGAAAAATGGCACGTTAACCGGTTATGCTTACGGGCTAGAGCTAAAACAAAAATTACTCAGTCTTGAGGGCTTATCAGT
TTAG

Upstream 100 bases:

>100_bases
GTGAAAATAGTGGTAGTGATTACTCAGCCATTAGCCAAAAGCTCGCTCAAATTAGTACCCCTTGGGGGAGCTATTTAACT
TTTCAGTTGTGGAACTTGTC

Downstream 100 bases:

>100_bases
AATAAATGGTTTATTTTCCTTTTAAACATAACTTGTAAGGCTCGGGAAGTTCCCTCAAGCTGCACACTAAGAAAGATAAG
CTTTTAAAGTGATATTAGCG

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MNKQSQPLVKLNFESDYGVISLCANETGITHLNFIPQIDSEWQSREVDNQLLENNRVQSSIGSVSEPALEQRASAHLLQA
KQELCEYFSGTRRHFDVALAPKGTEFQQQVWHALTELDYGQSCSYADIAMKIARPKAVRAVGTANGANPIAIIVPCHRVI
GKNGTLTGYAYGLELKQKLLSLEGLSV

Sequences:

>Translated_187_residues
MNKQSQPLVKLNFESDYGVISLCANETGITHLNFIPQIDSEWQSREVDNQLLENNRVQSSIGSVSEPALEQRASAHLLQA
KQELCEYFSGTRRHFDVALAPKGTEFQQQVWHALTELDYGQSCSYADIAMKIARPKAVRAVGTANGANPIAIIVPCHRVI
GKNGTLTGYAYGLELKQKLLSLEGLSV
>Mature_187_residues
MNKQSQPLVKLNFESDYGVISLCANETGITHLNFIPQIDSEWQSREVDNQLLENNRVQSSIGSVSEPALEQRASAHLLQA
KQELCEYFSGTRRHFDVALAPKGTEFQQQVWHALTELDYGQSCSYADIAMKIARPKAVRAVGTANGANPIAIIVPCHRVI
GKNGTLTGYAYGLELKQKLLSLEGLSV

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=185, Percent_Identity=28.1081081081081, Blast_Score=71, Evalue=7e-13,
Organism=Escherichia coli, GI1787596, Length=103, Percent_Identity=51.4563106796116, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1788542, Length=83, Percent_Identity=51.8072289156626, Blast_Score=95, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI115533070, Length=81, Percent_Identity=49.3827160493827, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115533068, Length=81, Percent_Identity=49.3827160493827, Blast_Score=85, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=46.9135802469136, Blast_Score=93, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17137554, Length=87, Percent_Identity=44.8275862068966, Blast_Score=74, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 20616; Mature: 20616

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQSQPLVKLNFESDYGVISLCANETGITHLNFIPQIDSEWQSREVDNQLLENNRVQSS
CCCCCCCEEEEEECCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHH
IGSVSEPALEQRASAHLLQAKQELCEYFSGTRRHFDVALAPKGTEFQQQVWHALTELDYG
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
QSCSYADIAMKIARPKAVRAVGTANGANPIAIIVPCHRVIGKNGTLTGYAYGLELKQKLL
CCCCHHHHHHHHHCCHHHHEEECCCCCCCEEEEEECHHHHCCCCCEEEEEECHHHHHHHH
SLEGLSV
HHCCCCC
>Mature Secondary Structure
MNKQSQPLVKLNFESDYGVISLCANETGITHLNFIPQIDSEWQSREVDNQLLENNRVQSS
CCCCCCCEEEEEECCCCCEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCHHHHH
IGSVSEPALEQRASAHLLQAKQELCEYFSGTRRHFDVALAPKGTEFQQQVWHALTELDYG
HCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCC
QSCSYADIAMKIARPKAVRAVGTANGANPIAIIVPCHRVIGKNGTLTGYAYGLELKQKLL
CCCCHHHHHHHHHCCHHHHEEECCCCCCCEEEEEECHHHHCCCCCEEEEEECHHHHHHHH
SLEGLSV
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]