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The map label for this gene is ada [H]

Identifier: 157961284

GI number: 157961284

Start: 1779534

End: 1781048

Strand: Direct

Name: ada [H]

Synonym: Spea_1458

Alternate gene names: 157961284

Gene position: 1779534-1781048 (Clockwise)

Preceding gene: 157961282

Following gene: 157961285

Centisome position: 34.39

GC content: 44.75

Gene sequence:

>1515_bases
ATGTTAGTTCAAGAGTGCCAGCTTGATAAAGACGCTTGCCGTAAAGCAAGGCTTGCTCGAGATCCGCGATTTGACGGCCA
GTTTTTTATTGGCGTGCTTACAACTAAAATTTATTGCAGACCAATATGCCCGGCAGTCAGTCCGAAAGAGCAAAATGTTC
GCTATTTTGATAATGCGATACAAGCTGCAAATGCCGGACTACGCCCGTGTTTACGTTGCCGACCAGATTCAGCCCCCAAG
TCTTATGCATGGCAAGGCACACAAACTACCTTAGACAGAGCTAAAGCATTAATTGATGCAGGCACGTTGTCAGGCACAGA
AGGGCAGAGTGTAGAAGCCTTATCTGAACGTCTTGGGATAAGTAGTCGCTACTTGCGTAGACTGTTTAACGATAAAATGG
GGATTTCCGTAAAAGGGTATAACCAGTATCGACAACTTCTGCTTGCGAAACAGTTATTACATCAGACTGAGTTACCAATC
ACCCAAGTGGCATTTGCGGCTGGCTTTACTAGTATTCGACGCTTTAATGAAGCTTTCCAGCAAACATTGCAGCTAACCCC
ATCAGAATTACGAAAAGAAAATGCTAAGCCTAATAGGGAACATCGAGTAAGTACTGAGCTAACACCTAATAGCACCAACC
AGAATAAAGGGTTAGTGGCGTTGAAACTACAGATGAGTTTCAGGCCACCTTTTTCATGGGCTAAGCAGCATCATTTTTAT
CAGGTGCGTGCCGTCACTCATATGGAGTGGCTAGCTGATGAACTTAGCTATGGTCGTAGCTTTACGATTAACGGGGTAAG
TGGTTATTTTTCCGCAAAGCTCGATGCTAGCAAGCATCAGTTTAACGTCGAGATTGTACTCGAGAATGAATCTGGGCTGG
TGCAGCTTAGTGCAGTCATTAATGGCATTCGCCGAGTGTTGGATCTTGATGCCAATATTAGTCAGATTGACAATGTGCTC
GAAACCATTACACCCCTAGCAGGACAAATGACTCAAGGGCTGAGACTACCTGGTGTTTGGTCTGTGTTTGAGGCTGGCTG
CAGGGCGGTTTTAGGTCAGCAAGTCAGTATTGTACAAGCTTCGAAGTTACTTAATCAGTTAGTGCTCGCTTATGGTGAAG
AGCGCCTTATTGGTGACAAACGTGTTAAGTTATTTCCAACGCCAGAAGTGATAGCCAAAGCGGAACTGACAGAGTTAAAA
ATCCCTGGTGCTAGAAGAAACGCCCTTAATGCGTTAGCTCAATTTGTCTATGAGCATCCGGATGCAGTACCTGATTCATG
GCTCACAATCAAAGGTATAGGGCCGTGGACTATTGCCTATGCAAAAATGCGTGGATTGAGCGATCCCAATATTCTGTTGT
GCGGCGATCTTGTCGTGAAAAAGCGAGTGTTGGCCTTATATTCTGAGCATAGTGAAAATAGTGGTAGTGATTACTCAGCC
ATTAGCCAAAAGCTCGCTCAAATTAGTACCCCTTGGGGGAGCTATTTAACTTTTCAGTTGTGGAACTTGTCATGA

Upstream 100 bases:

>100_bases
TAAATAAAGCAATGAATTAATATAGAGTTCCGTTTTCCGCTAGTGATGCAAAGGCTTGTTGCGTAAGATTGGTGTTATCA
ACCTAATTGTAGGACTGTAA

Downstream 100 bases:

>100_bases
ATAAACAATCACAACCTTTGGTGAAACTCAATTTTGAATCAGATTATGGAGTGATTTCCTTATGCGCAAATGAAACAGGG
ATCACTCATTTAAATTTTAT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MLVQECQLDKDACRKARLARDPRFDGQFFIGVLTTKIYCRPICPAVSPKEQNVRYFDNAIQAANAGLRPCLRCRPDSAPK
SYAWQGTQTTLDRAKALIDAGTLSGTEGQSVEALSERLGISSRYLRRLFNDKMGISVKGYNQYRQLLLAKQLLHQTELPI
TQVAFAAGFTSIRRFNEAFQQTLQLTPSELRKENAKPNREHRVSTELTPNSTNQNKGLVALKLQMSFRPPFSWAKQHHFY
QVRAVTHMEWLADELSYGRSFTINGVSGYFSAKLDASKHQFNVEIVLENESGLVQLSAVINGIRRVLDLDANISQIDNVL
ETITPLAGQMTQGLRLPGVWSVFEAGCRAVLGQQVSIVQASKLLNQLVLAYGEERLIGDKRVKLFPTPEVIAKAELTELK
IPGARRNALNALAQFVYEHPDAVPDSWLTIKGIGPWTIAYAKMRGLSDPNILLCGDLVVKKRVLALYSEHSENSGSDYSA
ISQKLAQISTPWGSYLTFQLWNLS

Sequences:

>Translated_504_residues
MLVQECQLDKDACRKARLARDPRFDGQFFIGVLTTKIYCRPICPAVSPKEQNVRYFDNAIQAANAGLRPCLRCRPDSAPK
SYAWQGTQTTLDRAKALIDAGTLSGTEGQSVEALSERLGISSRYLRRLFNDKMGISVKGYNQYRQLLLAKQLLHQTELPI
TQVAFAAGFTSIRRFNEAFQQTLQLTPSELRKENAKPNREHRVSTELTPNSTNQNKGLVALKLQMSFRPPFSWAKQHHFY
QVRAVTHMEWLADELSYGRSFTINGVSGYFSAKLDASKHQFNVEIVLENESGLVQLSAVINGIRRVLDLDANISQIDNVL
ETITPLAGQMTQGLRLPGVWSVFEAGCRAVLGQQVSIVQASKLLNQLVLAYGEERLIGDKRVKLFPTPEVIAKAELTELK
IPGARRNALNALAQFVYEHPDAVPDSWLTIKGIGPWTIAYAKMRGLSDPNILLCGDLVVKKRVLALYSEHSENSGSDYSA
ISQKLAQISTPWGSYLTFQLWNLS
>Mature_504_residues
MLVQECQLDKDACRKARLARDPRFDGQFFIGVLTTKIYCRPICPAVSPKEQNVRYFDNAIQAANAGLRPCLRCRPDSAPK
SYAWQGTQTTLDRAKALIDAGTLSGTEGQSVEALSERLGISSRYLRRLFNDKMGISVKGYNQYRQLLLAKQLLHQTELPI
TQVAFAAGFTSIRRFNEAFQQTLQLTPSELRKENAKPNREHRVSTELTPNSTNQNKGLVALKLQMSFRPPFSWAKQHHFY
QVRAVTHMEWLADELSYGRSFTINGVSGYFSAKLDASKHQFNVEIVLENESGLVQLSAVINGIRRVLDLDANISQIDNVL
ETITPLAGQMTQGLRLPGVWSVFEAGCRAVLGQQVSIVQASKLLNQLVLAYGEERLIGDKRVKLFPTPEVIAKAELTELK
IPGARRNALNALAQFVYEHPDAVPDSWLTIKGIGPWTIAYAKMRGLSDPNILLCGDLVVKKRVLALYSEHSENSGSDYSA
ISQKLAQISTPWGSYLTFQLWNLS

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=290, Percent_Identity=30, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI1788542, Length=174, Percent_Identity=32.7586206896552, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 56316; Mature: 56316

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVQECQLDKDACRKARLARDPRFDGQFFIGVLTTKIYCRPICPAVSPKEQNVRYFDNAI
CCCCHHCCCHHHHHHHHHHCCCCCCCCEEHEEHHHHHHHEECCCCCCCCCCCHHHHHHHH
QAANAGLRPCLRCRPDSAPKSYAWQGTQTTLDRAKALIDAGTLSGTEGQSVEALSERLGI
HHHCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
SSRYLRRLFNDKMGISVKGYNQYRQLLLAKQLLHQTELPITQVAFAAGFTSIRRFNEAFQ
HHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
QTLQLTPSELRKENAKPNREHRVSTELTPNSTNQNKGLVALKLQMSFRPPFSWAKQHHFY
HHHCCCHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHCCEE
QVRAVTHMEWLADELSYGRSFTINGVSGYFSAKLDASKHQFNVEIVLENESGLVQLSAVI
EEHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCEEEEEEEEECCCCCEEHHHHH
NGIRRVLDLDANISQIDNVLETITPLAGQMTQGLRLPGVWSVFEAGCRAVLGQQVSIVQA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
SKLLNQLVLAYGEERLIGDKRVKLFPTPEVIAKAELTELKIPGARRNALNALAQFVYEHP
HHHHHHHHHHHCCHHCCCCCCEEEECCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCC
DAVPDSWLTIKGIGPWTIAYAKMRGLSDPNILLCGDLVVKKRVLALYSEHSENSGSDYSA
CCCCCCCEEEECCCCHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCHHHH
ISQKLAQISTPWGSYLTFQLWNLS
HHHHHHHHCCCCCCEEEEEEECCC
>Mature Secondary Structure
MLVQECQLDKDACRKARLARDPRFDGQFFIGVLTTKIYCRPICPAVSPKEQNVRYFDNAI
CCCCHHCCCHHHHHHHHHHCCCCCCCCEEHEEHHHHHHHEECCCCCCCCCCCHHHHHHHH
QAANAGLRPCLRCRPDSAPKSYAWQGTQTTLDRAKALIDAGTLSGTEGQSVEALSERLGI
HHHCCCCCCHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCC
SSRYLRRLFNDKMGISVKGYNQYRQLLLAKQLLHQTELPITQVAFAAGFTSIRRFNEAFQ
HHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
QTLQLTPSELRKENAKPNREHRVSTELTPNSTNQNKGLVALKLQMSFRPPFSWAKQHHFY
HHHCCCHHHHHHCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEEEECCCCCHHHHHHCCEE
QVRAVTHMEWLADELSYGRSFTINGVSGYFSAKLDASKHQFNVEIVLENESGLVQLSAVI
EEHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEECCCCCEEEEEEEEECCCCCEEHHHHH
NGIRRVLDLDANISQIDNVLETITPLAGQMTQGLRLPGVWSVFEAGCRAVLGQQVSIVQA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHH
SKLLNQLVLAYGEERLIGDKRVKLFPTPEVIAKAELTELKIPGARRNALNALAQFVYEHP
HHHHHHHHHHHCCHHCCCCCCEEEECCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCC
DAVPDSWLTIKGIGPWTIAYAKMRGLSDPNILLCGDLVVKKRVLALYSEHSENSGSDYSA
CCCCCCCEEEECCCCHHHHHHHHHCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCHHHH
ISQKLAQISTPWGSYLTFQLWNLS
HHHHHHHHCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]