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The map label for this gene is nrfA [H]

Identifier: 157960659

GI number: 157960659

Start: 1030831

End: 1032234

Strand: Direct

Name: nrfA [H]

Synonym: Spea_0831

Alternate gene names: 157960659

Gene position: 1030831-1032234 (Clockwise)

Preceding gene: 157960656

Following gene: 157960661

Centisome position: 19.92

GC content: 44.02

Gene sequence:

>1404_bases
ATGGTGAAAACATTAACTAAAAAGTCTTTTGCACTCAGTGCAATCGTAGCTGCAAGTTTAATGGCTGGTAGCGCACTAGC
CAGTGATAAAACTGAACCTCGTAACGAAGTTTATAAAGATAAGTTTTCAAAGCAGTATAACAGCTGGCATGAAACTTCTG
AAAGTGTAGAGATCATTGATGCACTTGAGCAAGATCCAAATCTAGTTATTCTTTGGGCCGGTTATGGTTTTGCTAAAGAT
TATAACAAGGCTCGTGGTCATGTTTATGCAGTAACGGATCTGCGTAATACGCTACGCTCTGGTGCTCCTAAGACTGCTGA
AGATGGTCCAATGCCAATGGCATGTTGGTCTTGTAAGAGCCCTGATGTACCTCGTATGATCGAAGAGCAAGGTGAAGACG
GTTACTTCAAAGGTAAGTGGGCTAAAGGCGGTGCAGAAATCGTTAACTCTATCGGTTGTTCTGACTGTCACGAAAAAGGT
AAGTCTAAGCTACGTATCTCTCGTCCATTCGCTGAGCGCGGCATGGAAGCTCTTGGCACTCCATTCGATGAAGCTTCTCG
TAAAGACAAGCAATCAATGGTTTGTGGTCAGTGTCACGTAGAGTACTACTTCGAAAAGACTGCTGATAAGAAAGGCTTCG
TTAAGTTCCCATGGGACAAGGGCACAACTGTTGAAGATATGGAAGCATACTATGATGCTATCGATTTCGCAGATTGGACT
CACGCCGTTTCTAAGACGCCAATGCTAAAAGCACAGCACCCAGGTTATGAAACTTGGCAGCTAGGTATGCACGGTAAGAA
CAATGTAAGTTGTACTGACTGTCACATGCCTAAAGTTAAGAATGCTGAAGGCCGTAAGTTTACCGACCACAAAGTGGGTA
ACCCATTCGATCGTTTCGAAGAAACTTGTGGTACTTGTCATGATCAAAGCAAAGAGTTCATGGTTAACCTAGATAACGAG
CGTAAAGCTAAGGTTAAGCAGATCCAACTTCAAGCTGAAGATCAGTTAGTTAAAGCTCACTTCGAAGCTGGTGCAGCTTG
GGAAGCTGGCGCAACTGAAGCTGAAATGGCACCAATCCTAATGGATATCCGTCATGGTCAATGGCGTTGGGATTATGCAA
TCGCTTCTCACGGTGTTGCATCTCACGCTCCAGATGAAGCGTTACGTGTACTAGGTACTGCGCTAAACAAAGCTGCTGAT
GCTCGCGTTAAGCTAGCTCAACTTCTTGCTAAGAAAGGTGTTAAGCAGCCTATCGCGCTTCCAGATATCTCTACTAAAGC
TAAGGCTCAAGCTGCTCTAGGTATGGATATGAAGAAGATGAATGCTGATAAGGCTGAATTCAAGAAGACTATTCTACCTA
AGTGGGATGCTGAAGCTGAAAAGCGTGAAGCTACTTACAAGTAA

Upstream 100 bases:

>100_bases
ACCTCATAGGAGGTATGTCTTTGTTGCGTATAAGTGATTAAATTCAACCTTGAAATAGGTGGGACTATACAAAAGAATAT
AAAAGTAACATGGAGATGAG

Downstream 100 bases:

>100_bases
GTCTTACACTGCATAGTGTTTAATATTAAAAGCCCTCATTAGAGGGCTTTTTTATTTTCAGCCCTAAACCACCTACTTCA
GTAGGGGCCGTTATCATCAA

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase [H]

Number of amino acids: Translated: 467; Mature: 467

Protein sequence:

>467_residues
MVKTLTKKSFALSAIVAASLMAGSALASDKTEPRNEVYKDKFSKQYNSWHETSESVEIIDALEQDPNLVILWAGYGFAKD
YNKARGHVYAVTDLRNTLRSGAPKTAEDGPMPMACWSCKSPDVPRMIEEQGEDGYFKGKWAKGGAEIVNSIGCSDCHEKG
KSKLRISRPFAERGMEALGTPFDEASRKDKQSMVCGQCHVEYYFEKTADKKGFVKFPWDKGTTVEDMEAYYDAIDFADWT
HAVSKTPMLKAQHPGYETWQLGMHGKNNVSCTDCHMPKVKNAEGRKFTDHKVGNPFDRFEETCGTCHDQSKEFMVNLDNE
RKAKVKQIQLQAEDQLVKAHFEAGAAWEAGATEAEMAPILMDIRHGQWRWDYAIASHGVASHAPDEALRVLGTALNKAAD
ARVKLAQLLAKKGVKQPIALPDISTKAKAQAALGMDMKKMNADKAEFKKTILPKWDAEAEKREATYK

Sequences:

>Translated_467_residues
MVKTLTKKSFALSAIVAASLMAGSALASDKTEPRNEVYKDKFSKQYNSWHETSESVEIIDALEQDPNLVILWAGYGFAKD
YNKARGHVYAVTDLRNTLRSGAPKTAEDGPMPMACWSCKSPDVPRMIEEQGEDGYFKGKWAKGGAEIVNSIGCSDCHEKG
KSKLRISRPFAERGMEALGTPFDEASRKDKQSMVCGQCHVEYYFEKTADKKGFVKFPWDKGTTVEDMEAYYDAIDFADWT
HAVSKTPMLKAQHPGYETWQLGMHGKNNVSCTDCHMPKVKNAEGRKFTDHKVGNPFDRFEETCGTCHDQSKEFMVNLDNE
RKAKVKQIQLQAEDQLVKAHFEAGAAWEAGATEAEMAPILMDIRHGQWRWDYAIASHGVASHAPDEALRVLGTALNKAAD
ARVKLAQLLAKKGVKQPIALPDISTKAKAQAALGMDMKKMNADKAEFKKTILPKWDAEAEKREATYK
>Mature_467_residues
MVKTLTKKSFALSAIVAASLMAGSALASDKTEPRNEVYKDKFSKQYNSWHETSESVEIIDALEQDPNLVILWAGYGFAKD
YNKARGHVYAVTDLRNTLRSGAPKTAEDGPMPMACWSCKSPDVPRMIEEQGEDGYFKGKWAKGGAEIVNSIGCSDCHEKG
KSKLRISRPFAERGMEALGTPFDEASRKDKQSMVCGQCHVEYYFEKTADKKGFVKFPWDKGTTVEDMEAYYDAIDFADWT
HAVSKTPMLKAQHPGYETWQLGMHGKNNVSCTDCHMPKVKNAEGRKFTDHKVGNPFDRFEETCGTCHDQSKEFMVNLDNE
RKAKVKQIQLQAEDQLVKAHFEAGAAWEAGATEAEMAPILMDIRHGQWRWDYAIASHGVASHAPDEALRVLGTALNKAAD
ARVKLAQLLAKKGVKQPIALPDISTKAKAQAALGMDMKKMNADKAEFKKTILPKWDAEAEKREATYK

Specific function: Plays a role in nitrite reduction [H]

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family [H]

Homologues:

Organism=Escherichia coli, GI1790506, Length=438, Percent_Identity=62.1004566210046, Blast_Score=597, Evalue=1e-172,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031 [H]

Pfam domain/function: PF02335 Cytochrom_C552 [H]

EC number: =1.7.2.2 [H]

Molecular weight: Translated: 52049; Mature: 52049

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKTLTKKSFALSAIVAASLMAGSALASDKTEPRNEVYKDKFSKQYNSWHETSESVEIID
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
ALEQDPNLVILWAGYGFAKDYNKARGHVYAVTDLRNTLRSGAPKTAEDGPMPMACWSCKS
HHCCCCCEEEEECCCCCHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEEECCCC
PDVPRMIEEQGEDGYFKGKWAKGGAEIVNSIGCSDCHEKGKSKLRISRPFAERGMEALGT
CCCCHHHHHCCCCCEEECCCCCCHHHHHHHCCCHHHHHCCCCCEEEECCHHHHCHHHHCC
PFDEASRKDKQSMVCGQCHVEYYFEKTADKKGFVKFPWDKGTTVEDMEAYYDAIDFADWT
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
HAVSKTPMLKAQHPGYETWQLGMHGKNNVSCTDCHMPKVKNAEGRKFTDHKVGNPFDRFE
HHHCCCCCCCCCCCCCCEEEECCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHH
ETCGTCHDQSKEFMVNLDNERKAKVKQIQLQAEDQLVKAHFEAGAAWEAGATEAEMAPIL
HHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
MDIRHGQWRWDYAIASHGVASHAPDEALRVLGTALNKAADARVKLAQLLAKKGVKQPIAL
HHHCCCCEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDISTKAKAQAALGMDMKKMNADKAEFKKTILPKWDAEAEKREATYK
CCCCHHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCCCCHHHHCCCCC
>Mature Secondary Structure
MVKTLTKKSFALSAIVAASLMAGSALASDKTEPRNEVYKDKFSKQYNSWHETSESVEIID
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHH
ALEQDPNLVILWAGYGFAKDYNKARGHVYAVTDLRNTLRSGAPKTAEDGPMPMACWSCKS
HHCCCCCEEEEECCCCCHHHHHHCCCCEEEHHHHHHHHHCCCCCCCCCCCCCEEEECCCC
PDVPRMIEEQGEDGYFKGKWAKGGAEIVNSIGCSDCHEKGKSKLRISRPFAERGMEALGT
CCCCHHHHHCCCCCEEECCCCCCHHHHHHHCCCHHHHHCCCCCEEEECCHHHHCHHHHCC
PFDEASRKDKQSMVCGQCHVEYYFEKTADKKGFVKFPWDKGTTVEDMEAYYDAIDFADWT
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
HAVSKTPMLKAQHPGYETWQLGMHGKNNVSCTDCHMPKVKNAEGRKFTDHKVGNPFDRFE
HHHCCCCCCCCCCCCCCEEEECCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHHH
ETCGTCHDQSKEFMVNLDNERKAKVKQIQLQAEDQLVKAHFEAGAAWEAGATEAEMAPIL
HHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHH
MDIRHGQWRWDYAIASHGVASHAPDEALRVLGTALNKAADARVKLAQLLAKKGVKQPIAL
HHHCCCCEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PDISTKAKAQAALGMDMKKMNADKAEFKKTILPKWDAEAEKREATYK
CCCCHHHHHHHHHCCCHHHHCCCHHHHHHHCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA