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The map label for this gene is glnK [H]

Identifier: 157960661

GI number: 157960661

Start: 1033605

End: 1033943

Strand: Direct

Name: glnK [H]

Synonym: Spea_0833

Alternate gene names: 157960661

Gene position: 1033605-1033943 (Clockwise)

Preceding gene: 157960659

Following gene: 157960662

Centisome position: 19.97

GC content: 46.61

Gene sequence:

>339_bases
ATGAAATTGGTTAGCGCGATCATTAAGCCATTTAAATTAGACGATGTACGAGAGGCTGTGGCTGGTGTCGGTATTGAAGG
CATGACGGTGACAGAGGTGAAGGGCTTTGGACGTCAAAAAGGTCACACCGAGCTATATCGTGGCGCCGAGTATCAAGTGG
ATTTCTTACCTAAGGTAAAGCTTGAGATTGCAATTAAGGCTGACAGGTTAGAGATGCTACTTGAAGCGATAACTGGCGCG
GCTCGAACCGGGAAGATAGGTGACGGTAAGATTTTTGTGACAGATCTTGAGCAAGCTATCCGGATCAGAACTGGCGAACA
TGATAACGACGCACTTTAA

Upstream 100 bases:

>100_bases
TGTCGTGATTCACTTATCTCCTTGAATTTGCGTGATTAGATATTCTGGCCTAATGCTTGAATTATGTTTTAGTAAATTGA
AAAAGGAATTGGAGTGAGTC

Downstream 100 bases:

>100_bases
GGGGATAAGCGAATGGATGATTTAACCAAATTGGGATTGACGGTAACTGAGTTACGCTTCGCCCTAGATACGTTTTACTT
TTTGATTTCAGGTGCACTCG

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 112; Mature: 112

Protein sequence:

>112_residues
MKLVSAIIKPFKLDDVREAVAGVGIEGMTVTEVKGFGRQKGHTELYRGAEYQVDFLPKVKLEIAIKADRLEMLLEAITGA
ARTGKIGDGKIFVTDLEQAIRIRTGEHDNDAL

Sequences:

>Translated_112_residues
MKLVSAIIKPFKLDDVREAVAGVGIEGMTVTEVKGFGRQKGHTELYRGAEYQVDFLPKVKLEIAIKADRLEMLLEAITGA
ARTGKIGDGKIFVTDLEQAIRIRTGEHDNDAL
>Mature_112_residues
MKLVSAIIKPFKLDDVREAVAGVGIEGMTVTEVKGFGRQKGHTELYRGAEYQVDFLPKVKLEIAIKADRLEMLLEAITGA
ARTGKIGDGKIFVTDLEQAIRIRTGEHDNDAL

Specific function: P-II indirectly controls the transcription of the glutamine synthetase gene (glnA). P-II prevents NR-II-catalyzed conversion of NR-I to NR-I-phosphate, the transcriptional activator of glnA. When P-II is uridylylated to P-II-UMP, these events are reversed

COG id: COG0347

COG function: function code E; Nitrogen regulatory protein PII

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=67.8571428571429, Blast_Score=161, Evalue=1e-41,
Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=70.5357142857143, Blast_Score=159, Evalue=6e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12297; Mature: 12297

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00496 PII_GLNB_UMP ; PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVSAIIKPFKLDDVREAVAGVGIEGMTVTEVKGFGRQKGHTELYRGAEYQVDFLPKVK
CCHHHHHHCCCCCHHHHHHHHCCCCCCEEEEHCCCCCCCCCCHHHHCCCCCEEECCCCEE
LEIAIKADRLEMLLEAITGAARTGKIGDGKIFVTDLEQAIRIRTGEHDNDAL
EEEEEEHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKLVSAIIKPFKLDDVREAVAGVGIEGMTVTEVKGFGRQKGHTELYRGAEYQVDFLPKVK
CCHHHHHHCCCCCHHHHHHHHCCCCCCEEEEHCCCCCCCCCCHHHHCCCCCEEECCCCEE
LEIAIKADRLEMLLEAITGAARTGKIGDGKIFVTDLEQAIRIRTGEHDNDAL
EEEEEEHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]