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The map label for this gene is ahpF [H]

Identifier: 157960575

GI number: 157960575

Start: 923915

End: 925489

Strand: Direct

Name: ahpF [H]

Synonym: Spea_0747

Alternate gene names: 157960575

Gene position: 923915-925489 (Clockwise)

Preceding gene: 157960574

Following gene: 157960576

Centisome position: 17.85

GC content: 48.63

Gene sequence:

>1575_bases
ATGTTAGATGCAAACGTAAAGAATCAACTGAACACTTATTTGCAGAACCTCAAGCATCCAGTTGAGCTGGTTGTTTCTAT
TGATGACAGTAAAAAAGCTCAAGAGCTTAAGTCGTTAGCTACGGATATCGATAGCCTGTCTTCACTTATCTCTGTAACAG
ATCAAGTCGGCGAGCGTACGCCATCAATGTCGGTAATTAACCCGCAATTGAATAGCAAAATTACTTTTGCAGGCCTGCCT
ATGGGTCATGAGTTTACCTCTTTGGTACTTGCGCTTCTGCACAGTGGCGGCCACCCAATCAAGCTTGAAGCCGATGTGAT
TGAGCAAATTCGTCAGCTACCGGGTGAGTATCGCTTCGAGACCTACGTCTCTCTAAGTTGTCAGACTTGCCCTGAAGTGG
TTCAGGCGCTGAACATGATGGCGGCAATTAACCCAAATATCACTAACGTGATGATCGACGGATCACTGTTCCAAGACGAA
GTGAATGAGCGCAACATCATGGCGGTGCCATCTGTGTACTTAAATGGTGAGCAGTTCTCAATGGGCGCTATCAGCACGGT
TGAGATCTTAAATAAGCTTGATGTAAACGCAGCAGGTAGAAAAGCCGAGCAGCTAAACGAGAAAGAGCCATTCGAAGTAT
TAGTAATTGGCGGTGGCCCAGCGGGGGCATCGGCGGCAATCTACGCGGCACGTAAAGGTCTGCGCACCGGTATTGTAGCA
GACAAGTTTGGTGGTCAGGTTGCTGAAACTGTTGGCATTGAGAACTTCATCTCTGTTAAAGCGACCGAAGGTCCAAAGCT
GGTGGCAAATCTTGAAGCCCACGTACATGAATATGATGTCGATATCATGAACAACCAGCGTGCCTTAAGCCTTAAGTCTG
GTGAGTTATTCGAACTGGAACTAGAAAATGGCGCGGTACTGCGCAGCAAAACAGTATTGCTAGCAACCGGTGCTCGTTGG
CGTGAAATGAACGTGCCAGGTGAGCAAGAATATCGCGGCAGTGGCGTTGCATACTGCCCACACTGTGATGGTCCACTGTT
CAAAGGCAAGCGCGTTGCGGTGATAGGTGGCGGTAACTCAGGAATCGAAGCGGCAATCGATTTAGCCAATATTGTTGAAC
ACGTTACTGTGCTTGAGTTTGATAGCAGTCTACGCGCCGATGAAGTGTTGCAGCGCAAAGCTAAGTCTATGGGCAATATC
GATATCATCACCCAAGCAATGACAACCGAGGTCACTGGCGATGGTAAGCGCGTTAACGGCCTTAACTATACCGACAGAGC
GACAGGCGAAAGCCATCATGTTGAGCTTGCTGGAATATTTGTGCAGATAGGTTTAGTACCCAACTCTGAGTGGCTTAAAG
ACACTATCGATATGACGGCGCGCGGCGAAATCATTGTCGACGGCAAAGGCGAGACCTCTGTGCCGGGTATTTTTGCGGCG
GGTGATGTGACTAACTCGGCCTACAAACAGATCATCATTGCCATGGGCAGCGGAGCAACCGCATCGTTAGGCGCATTTGA
TTACTTAATTCGTCACGATATCGATGAGTCGGCGGGTGGCAATATTGCTGCCTAG

Upstream 100 bases:

>100_bases
CATTTTTTATTTTTATCGCGAGTGCTAGGTATGCGCCAAGGCTTGTCGCTGCCTGCAAAACGTGAATTTCAGATTAGTTT
TAAGATATTTAGGAGTCATC

Downstream 100 bases:

>100_bases
ATAGCAAATTCTTATTGAAAAGTCAGCAAATGCTGGCTTTTTTTATGCTTTAACAGTATTAATAGCTCCGTTAATAGTGA
CATTTCTTATAGGCTAAATA

Product: FAD-dependent pyridine nucleotide-disulfide oxidoreductase

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 524

Protein sequence:

>524_residues
MLDANVKNQLNTYLQNLKHPVELVVSIDDSKKAQELKSLATDIDSLSSLISVTDQVGERTPSMSVINPQLNSKITFAGLP
MGHEFTSLVLALLHSGGHPIKLEADVIEQIRQLPGEYRFETYVSLSCQTCPEVVQALNMMAAINPNITNVMIDGSLFQDE
VNERNIMAVPSVYLNGEQFSMGAISTVEILNKLDVNAAGRKAEQLNEKEPFEVLVIGGGPAGASAAIYAARKGLRTGIVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVHEYDVDIMNNQRALSLKSGELFELELENGAVLRSKTVLLATGARW
REMNVPGEQEYRGSGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDSSLRADEVLQRKAKSMGNI
DIITQAMTTEVTGDGKRVNGLNYTDRATGESHHVELAGIFVQIGLVPNSEWLKDTIDMTARGEIIVDGKGETSVPGIFAA
GDVTNSAYKQIIIAMGSGATASLGAFDYLIRHDIDESAGGNIAA

Sequences:

>Translated_524_residues
MLDANVKNQLNTYLQNLKHPVELVVSIDDSKKAQELKSLATDIDSLSSLISVTDQVGERTPSMSVINPQLNSKITFAGLP
MGHEFTSLVLALLHSGGHPIKLEADVIEQIRQLPGEYRFETYVSLSCQTCPEVVQALNMMAAINPNITNVMIDGSLFQDE
VNERNIMAVPSVYLNGEQFSMGAISTVEILNKLDVNAAGRKAEQLNEKEPFEVLVIGGGPAGASAAIYAARKGLRTGIVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVHEYDVDIMNNQRALSLKSGELFELELENGAVLRSKTVLLATGARW
REMNVPGEQEYRGSGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDSSLRADEVLQRKAKSMGNI
DIITQAMTTEVTGDGKRVNGLNYTDRATGESHHVELAGIFVQIGLVPNSEWLKDTIDMTARGEIIVDGKGETSVPGIFAA
GDVTNSAYKQIIIAMGSGATASLGAFDYLIRHDIDESAGGNIAA
>Mature_524_residues
MLDANVKNQLNTYLQNLKHPVELVVSIDDSKKAQELKSLATDIDSLSSLISVTDQVGERTPSMSVINPQLNSKITFAGLP
MGHEFTSLVLALLHSGGHPIKLEADVIEQIRQLPGEYRFETYVSLSCQTCPEVVQALNMMAAINPNITNVMIDGSLFQDE
VNERNIMAVPSVYLNGEQFSMGAISTVEILNKLDVNAAGRKAEQLNEKEPFEVLVIGGGPAGASAAIYAARKGLRTGIVA
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVHEYDVDIMNNQRALSLKSGELFELELENGAVLRSKTVLLATGARW
REMNVPGEQEYRGSGVAYCPHCDGPLFKGKRVAVIGGGNSGIEAAIDLANIVEHVTVLEFDSSLRADEVLQRKAKSMGNI
DIITQAMTTEVTGDGKRVNGLNYTDRATGESHHVELAGIFVQIGLVPNSEWLKDTIDMTARGEIIVDGKGETSVPGIFAA
GDVTNSAYKQIIIAMGSGATASLGAFDYLIRHDIDESAGGNIAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=517, Percent_Identity=56.0928433268859, Blast_Score=598, Evalue=1e-172,
Organism=Escherichia coli, GI1787114, Length=309, Percent_Identity=33.0097087378641, Blast_Score=160, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6321898, Length=334, Percent_Identity=33.2335329341317, Blast_Score=166, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6320560, Length=311, Percent_Identity=34.7266881028939, Blast_Score=164, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56148; Mature: 56148

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDANVKNQLNTYLQNLKHPVELVVSIDDSKKAQELKSLATDIDSLSSLISVTDQVGERT
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSMSVINPQLNSKITFAGLPMGHEFTSLVLALLHSGGHPIKLEADVIEQIRQLPGEYRFE
CCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCEEE
TYVSLSCQTCPEVVQALNMMAAINPNITNVMIDGSLFQDEVNERNIMAVPSVYLNGEQFS
EEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCEEEECEEEECCCEEC
MGAISTVEILNKLDVNAAGRKAEQLNEKEPFEVLVIGGGPAGASAAIYAARKGLRTGIVA
CCCHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEE
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVHEYDVDIMNNQRALSLKSGELFELE
HHCCCHHHHHHCCCCEEEEEECCCCEEEEEHHHHHEEEEEEEECCCEEEEECCCCEEEEE
LENGAVLRSKTVLLATGARWREMNVPGEQEYRGSGVAYCPHCDGPLFKGKRVAVIGGGNS
ECCCEEEEECEEEEEECCCEEECCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCC
GIEAAIDLANIVEHVTVLEFDSSLRADEVLQRKAKSMGNIDIITQAMTTEVTGDGKRVNG
CHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEHEEEECCCCCEECC
LNYTDRATGESHHVELAGIFVQIGLVPNSEWLKDTIDMTARGEIIVDGKGETSVPGIFAA
CCCCCCCCCCCCEEEEEEEEEEEEECCCHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEE
GDVTNSAYKQIIIAMGSGATASLGAFDYLIRHDIDESAGGNIAA
CCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLDANVKNQLNTYLQNLKHPVELVVSIDDSKKAQELKSLATDIDSLSSLISVTDQVGERT
CCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PSMSVINPQLNSKITFAGLPMGHEFTSLVLALLHSGGHPIKLEADVIEQIRQLPGEYRFE
CCCEEECCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHCCCCCEEE
TYVSLSCQTCPEVVQALNMMAAINPNITNVMIDGSLFQDEVNERNIMAVPSVYLNGEQFS
EEEEEEHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHCCCCCEEEECEEEECCCEEC
MGAISTVEILNKLDVNAAGRKAEQLNEKEPFEVLVIGGGPAGASAAIYAARKGLRTGIVA
CCCHHHHHHHHHCCCCCCCCHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCEE
DKFGGQVAETVGIENFISVKATEGPKLVANLEAHVHEYDVDIMNNQRALSLKSGELFELE
HHCCCHHHHHHCCCCEEEEEECCCCEEEEEHHHHHEEEEEEEECCCEEEEECCCCEEEEE
LENGAVLRSKTVLLATGARWREMNVPGEQEYRGSGVAYCPHCDGPLFKGKRVAVIGGGNS
ECCCEEEEECEEEEEECCCEEECCCCCCCCCCCCCEEECCCCCCCCCCCCEEEEEECCCC
GIEAAIDLANIVEHVTVLEFDSSLRADEVLQRKAKSMGNIDIITQAMTTEVTGDGKRVNG
CHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHCCCEEEEEEEHEEEECCCCCEECC
LNYTDRATGESHHVELAGIFVQIGLVPNSEWLKDTIDMTARGEIIVDGKGETSVPGIFAA
CCCCCCCCCCCCEEEEEEEEEEEEECCCHHHHHHHHCCCCCCCEEECCCCCCCCCCEEEE
GDVTNSAYKQIIIAMGSGATASLGAFDYLIRHDIDESAGGNIAA
CCCCHHHHEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]