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The map label for this gene is metF [H]

Identifier: 157960391

GI number: 157960391

Start: 690542

End: 691432

Strand: Direct

Name: metF [H]

Synonym: Spea_0562

Alternate gene names: 157960391

Gene position: 690542-691432 (Clockwise)

Preceding gene: 157960390

Following gene: 157960392

Centisome position: 13.34

GC content: 48.04

Gene sequence:

>891_bases
ATGGGTTTTCATCACGCACAACATGCAACATCATTAAACCAGAGCCTGTCTGAGCTAAATGGTGATATTAACGTTTCTTT
CGAATTTTTCCCGCCTTCAACTCCAGAGATGGAAAAGTTACTGTGGAACTCTATCCGTCGTTTAGAGCCATTAAATCCAA
AGTTTGTTTCGGTGACTTATGGTGCTAACTCGGGTGTTCGTGATAGAACCCATGGCGTAATTGAGCGTATCCAGACTGAA
ACAGACTTGCTGGCTGCGCCGCACCTGACTTTAGTCGATGCCAGCGATGAAGAGCTTAAAGAGCTGGCAAAACATTATTG
GAATAGCGGGATCCGCGATATCGTCGCCCTGCGCGGTGACTTACCAGACGGTAGCCCTAAGCCTACTCGTTTTGCTAATG
ATCTTGTCCGCCTATTACGTTCGGTAGCCGACTTTGATATCTCGGTGGCAGCCTACCCAGAGGTGCATCCCGATGCGAGT
AATGCACAGTCAGATCTGATTAATCTTAAAAAGAAGATAGACGCTGGTGCCAACCGCGCTATCACTCAGTTTTTCTTCGA
TGTGGAGTCATATCTGCGTTTTAGAGACCGCTGCGCGGCTGCAGGAATCGACGCTGAAATCATCCCAGGGATCTTACCTG
TGACTAACTTTAATCAGACTAAGCGCTTTGCCGCCATGACCAATGTGTCCATCCCAAGCTGGTTACATCGTCAGTTTGAG
GGGCTCGAAGATGATGCCATTACCCGCCAGATGGTTGGGGCTAACGTGGCTATCGACATGGTCAAAGTCCTATCTCGTGA
AGGAGTGAAAGACTTCCACTTCTATACGTTAAATCGCGCTGAGCTGACCTATGCCATTTGTCACACATTAGGGGTTAGAC
CGGGAAAGTAA

Upstream 100 bases:

>100_bases
GCTTGACTTCAATCTCAGTTTCTCTAGTATATGGACATATAGACGTCCAAACGTCTGTTTCTGGAAGTGCTAAATTAATG
CTGAAGTTGAGGAAGTAGAA

Downstream 100 bases:

>100_bases
GTGTCATAAACCCTTAAGTGATTTATTGAGCCAATAACCTCGTAGGTTGTTGGCTTTTTGTTTTAGAGAGGCATCCAGAA
GGCTGTGTATGCAGGAGCTT

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MGFHHAQHATSLNQSLSELNGDINVSFEFFPPSTPEMEKLLWNSIRRLEPLNPKFVSVTYGANSGVRDRTHGVIERIQTE
TDLLAAPHLTLVDASDEELKELAKHYWNSGIRDIVALRGDLPDGSPKPTRFANDLVRLLRSVADFDISVAAYPEVHPDAS
NAQSDLINLKKKIDAGANRAITQFFFDVESYLRFRDRCAAAGIDAEIIPGILPVTNFNQTKRFAAMTNVSIPSWLHRQFE
GLEDDAITRQMVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPGK

Sequences:

>Translated_296_residues
MGFHHAQHATSLNQSLSELNGDINVSFEFFPPSTPEMEKLLWNSIRRLEPLNPKFVSVTYGANSGVRDRTHGVIERIQTE
TDLLAAPHLTLVDASDEELKELAKHYWNSGIRDIVALRGDLPDGSPKPTRFANDLVRLLRSVADFDISVAAYPEVHPDAS
NAQSDLINLKKKIDAGANRAITQFFFDVESYLRFRDRCAAAGIDAEIIPGILPVTNFNQTKRFAAMTNVSIPSWLHRQFE
GLEDDAITRQMVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPGK
>Mature_295_residues
GFHHAQHATSLNQSLSELNGDINVSFEFFPPSTPEMEKLLWNSIRRLEPLNPKFVSVTYGANSGVRDRTHGVIERIQTET
DLLAAPHLTLVDASDEELKELAKHYWNSGIRDIVALRGDLPDGSPKPTRFANDLVRLLRSVADFDISVAAYPEVHPDASN
AQSDLINLKKKIDAGANRAITQFFFDVESYLRFRDRCAAAGIDAEIIPGILPVTNFNQTKRFAAMTNVSIPSWLHRQFEG
LEDDAITRQMVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPGK

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=285, Percent_Identity=30.8771929824561, Blast_Score=135, Evalue=4e-32,
Organism=Escherichia coli, GI1790377, Length=295, Percent_Identity=72.5423728813559, Blast_Score=461, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI25147436, Length=278, Percent_Identity=31.6546762589928, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25147441, Length=278, Percent_Identity=31.6546762589928, Blast_Score=127, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6325234, Length=294, Percent_Identity=31.6326530612245, Blast_Score=142, Evalue=9e-35,
Organism=Saccharomyces cerevisiae, GI6321313, Length=290, Percent_Identity=29.3103448275862, Blast_Score=128, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24662658, Length=260, Percent_Identity=26.1538461538462, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 33089; Mature: 32958

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFHHAQHATSLNQSLSELNGDINVSFEFFPPSTPEMEKLLWNSIRRLEPLNPKFVSVTY
CCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
GANSGVRDRTHGVIERIQTETDLLAAPHLTLVDASDEELKELAKHYWNSGIRDIVALRGD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHCC
LPDGSPKPTRFANDLVRLLRSVADFDISVAAYPEVHPDASNAQSDLINLKKKIDAGANRA
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHH
ITQFFFDVESYLRFRDRCAAAGIDAEIIPGILPVTNFNQTKRFAAMTNVSIPSWLHRQFE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHC
GLEDDAITRQMVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPGK
CCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GFHHAQHATSLNQSLSELNGDINVSFEFFPPSTPEMEKLLWNSIRRLEPLNPKFVSVTY
CCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEE
GANSGVRDRTHGVIERIQTETDLLAAPHLTLVDASDEELKELAKHYWNSGIRDIVALRGD
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHCC
LPDGSPKPTRFANDLVRLLRSVADFDISVAAYPEVHPDASNAQSDLINLKKKIDAGANRA
CCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHH
ITQFFFDVESYLRFRDRCAAAGIDAEIIPGILPVTNFNQTKRFAAMTNVSIPSWLHRQFE
HHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHC
GLEDDAITRQMVGANVAIDMVKVLSREGVKDFHFYTLNRAELTYAICHTLGVRPGK
CCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6356036; 8346018; 9278503; 10201405 [H]