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The map label for this gene is metL [H]

Identifier: 157960390

GI number: 157960390

Start: 687943

End: 690336

Strand: Direct

Name: metL [H]

Synonym: Spea_0561

Alternate gene names: 157960390

Gene position: 687943-690336 (Clockwise)

Preceding gene: 157960389

Following gene: 157960391

Centisome position: 13.29

GC content: 48.87

Gene sequence:

>2394_bases
ATGGCACGCTGTCACTTACATAAATTTGGTGGCTCAAGTCTTGCTGATGCAGACTGTTACCGTCGCGTGGCTCATATTCT
GCTGACTCACGGTCACAGCGATGACTTAGTTGTGGTGTCTGCGGCAGGTAAAAGCACCAATTTCTTATATAAGTTACTCG
AGTTGAGTACGACTAAGCAGCTGTGGCAAGAAGAGCTACAAACACTGATTAGTTTCCAGCAAAATTTGATTGAGCAATTG
CTCTCAAATGAGCAGGCACGCTCTCTTCGGGAGCGACTCTCTAACGATAAAGCGCAGCTAATCAGCTTGCTATCGATAGA
GTCGATTAATGAATACCAAAGCAATCAGGTAGTGAGCTTTGGCGAGCGTTGGTCTGCACGCCTATTGGCGGCGCTATTAC
GTGAATCGGGTGTGGCGGCATCGGATGTAGATGCTCGCACACTCTTGATTGCCGACGAAGCGGCTGTACCGCAGATTCGT
TTACAGGATTCCCGTCAACGTGTGCATTCGATGACTGAAACTCATCCTAATGAGCGTCTAATTATTACTGGCTTTATCTG
CGCGAACGAGCGGGGTGACACCTTGCTACTAGGACGTAACGGCTCCGATTTCAGTGCGACCTTGATTGCCAGCTTAGCAG
ATATTGAGCGCGTGACCATTTGGACCGATGTAGAGGGCGTATTTAACGCCGACCCAAATAAGATCAACGATGCTAAGTTA
CTGAGTAGTTTGTCATTAGCCGAAGCTAACCGCTTGGCACATCTAGGTTCACCTGTGCTGCATAATAGAACGCTGCAACC
GCTGTTTGATACTCAAGTGAGTTTGGCTGTTCGTTCTAGTTACGCGTCCCATACCGACTTTACCTTGATTGCACCAAAGA
GTTCGTCGGCTAGTGCGCCAGTGGTTACTAATCTAAGCAGCGTTGCGCTGTTCAATCTTGAGCTTACAACGGAACTTAAG
CAGCTTATTGAGCTGCTCGAGGAGACTGGCCTGAGTCCGCTGGCATACTGGGAACTTGGGCAGGGGCGCGCCGAGATCAC
TGTGACCTTAGAAAATCAGAAGCAGGTATTGGCATTACTGGATATTAACCGTGACGCCTTAGGCGTGAGTGATATTAAGA
TCACCGAAGATCTTGGCCTGGTTGCGCTGGTCAGCGCCGATGCCAGCCTGTATCGCCGCGGCTTTGCAAGATTACTGAGT
CGTAATGCTAGACCGCTGTTTAACGACGGCATGAGCCTAGTGACCTTGGTACCTCAAGGGCAAGTAAACTTGTTAACTCA
AAAAGTACACCGTCGCTGCGCGGGGCCTCGCAAACGTATCGGCGTACTGCTATTGGGTGTCGGTAACATTGGCGAAGCTT
GGGTTGAACTCTTTAGTGGCGCTAAGGCAGCACTCAATAAAGAGCTCGAGGCTAGTGTAGAGTTGGTTGGTTTGGTTAGC
TCACGTAACGCTTTGATCTGCGATGAGGGCATTGAGCTCGATTCGTGGAAGCAGGCCTTCGAAGAACACGCCACTCCATG
GCAATACAGCCATCTGTTTGAAGAGTTACATGATTTAGAGTGTGATGAAGTGATTGCGCTGGATATTAGTGCCAGCGCCG
ATTTGACCTTGCAATATCCAGAGTTTTTCGCTCGTGGTATTCATGTTGTCAGCGCTAATAAATTAGCCGGTTCTGGACCA
CTAACCTTCTATCGTGAATTGAAACAGCAATTGGATAACCGTCGCTTATTTTGGCGTTACAACGCCAGTTGTGGCGCTGG
TTTACCGGTACAACATGCATTGAACGATCTGCATAACAGCGGCGACAGTATCGAAGCTGTTGGCGGGATCTTCTCTGGCA
CTCTTTGTTGGTTATTTGAAAACTTCGATGCCAAGAAACCTTTCTCTGAGTTAGTTATTGAGGCGAGGGGCTTAGGGATC
ACCGAGCCAGATCCACGTGATGATCTGTCGGGTCGAGACATGCAGCGTAAACTACTCATTCTTGCCCGCGAGATAGGTTT
TGAGATTGAGCTTGAAGATATTGAGCTTAAATCTTTAGTGCCAGCAAACTTGGCTGAAATCCCTCTAGAGCAGTTTTTAT
CGCGTATCACCGAGCTCGATGATGAGATGCAGCAGCAGTTTATTGCGGCTGCAGAGCAAGATAAGGTGCTGAGATACGTG
GCCTCTTTAGATAAGGTCGATGGCGAGTTAAAAGCCGAGGTGGGGATACAGTGGGTTGATATCAGCCACCCCTATGCGAA
CCTAACGCCAGGTGACAACGTGTTTGTGATCCGCAGTGAATTCTATCAGGAAAATCCGTTGATTATTCGTGGCCCTGGAG
CCGGGCGTTTAGTGACAGCAGCGGCTGTACAGTCTGATCTGGCACATATCTGTCGCGATCTGCTACAAGAATAA

Upstream 100 bases:

>100_bases
TATTCGTCTGTCTGTGGGTATCGAAGATGCCGATGATCTTGTTGCCGATATTGAAGCTGCGCTTAATGCCGCAGCCGCTT
GTTAATTAAAGGGGTATACG

Downstream 100 bases:

>100_bases
TTTTCACGTTAATCAATAGAAAGGGCTCATTTGAGCCCTTTTTTATTGACTTGTTTATTATGATTTTATTGTTTTAATAA
AAAAACTCATTTGCCTGCTT

Product: bifunctional aspartate kinase II/homoserine dehydrogenase II

Products: NA

Alternate protein names: Aspartokinase II/homoserine dehydrogenase II; AKII-HDII; Aspartokinase; Homoserine dehydrogenase [H]

Number of amino acids: Translated: 797; Mature: 796

Protein sequence:

>797_residues
MARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYKLLELSTTKQLWQEELQTLISFQQNLIEQL
LSNEQARSLRERLSNDKAQLISLLSIESINEYQSNQVVSFGERWSARLLAALLRESGVAASDVDARTLLIADEAAVPQIR
LQDSRQRVHSMTETHPNERLIITGFICANERGDTLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKL
LSSLSLAEANRLAHLGSPVLHNRTLQPLFDTQVSLAVRSSYASHTDFTLIAPKSSSASAPVVTNLSSVALFNLELTTELK
QLIELLEETGLSPLAYWELGQGRAEITVTLENQKQVLALLDINRDALGVSDIKITEDLGLVALVSADASLYRRGFARLLS
RNARPLFNDGMSLVTLVPQGQVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWVELFSGAKAALNKELEASVELVGLVS
SRNALICDEGIELDSWKQAFEEHATPWQYSHLFEELHDLECDEVIALDISASADLTLQYPEFFARGIHVVSANKLAGSGP
LTFYRELKQQLDNRRLFWRYNASCGAGLPVQHALNDLHNSGDSIEAVGGIFSGTLCWLFENFDAKKPFSELVIEARGLGI
TEPDPRDDLSGRDMQRKLLILAREIGFEIELEDIELKSLVPANLAEIPLEQFLSRITELDDEMQQQFIAAAEQDKVLRYV
ASLDKVDGELKAEVGIQWVDISHPYANLTPGDNVFVIRSEFYQENPLIIRGPGAGRLVTAAAVQSDLAHICRDLLQE

Sequences:

>Translated_797_residues
MARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYKLLELSTTKQLWQEELQTLISFQQNLIEQL
LSNEQARSLRERLSNDKAQLISLLSIESINEYQSNQVVSFGERWSARLLAALLRESGVAASDVDARTLLIADEAAVPQIR
LQDSRQRVHSMTETHPNERLIITGFICANERGDTLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKL
LSSLSLAEANRLAHLGSPVLHNRTLQPLFDTQVSLAVRSSYASHTDFTLIAPKSSSASAPVVTNLSSVALFNLELTTELK
QLIELLEETGLSPLAYWELGQGRAEITVTLENQKQVLALLDINRDALGVSDIKITEDLGLVALVSADASLYRRGFARLLS
RNARPLFNDGMSLVTLVPQGQVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWVELFSGAKAALNKELEASVELVGLVS
SRNALICDEGIELDSWKQAFEEHATPWQYSHLFEELHDLECDEVIALDISASADLTLQYPEFFARGIHVVSANKLAGSGP
LTFYRELKQQLDNRRLFWRYNASCGAGLPVQHALNDLHNSGDSIEAVGGIFSGTLCWLFENFDAKKPFSELVIEARGLGI
TEPDPRDDLSGRDMQRKLLILAREIGFEIELEDIELKSLVPANLAEIPLEQFLSRITELDDEMQQQFIAAAEQDKVLRYV
ASLDKVDGELKAEVGIQWVDISHPYANLTPGDNVFVIRSEFYQENPLIIRGPGAGRLVTAAAVQSDLAHICRDLLQE
>Mature_796_residues
ARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYKLLELSTTKQLWQEELQTLISFQQNLIEQLL
SNEQARSLRERLSNDKAQLISLLSIESINEYQSNQVVSFGERWSARLLAALLRESGVAASDVDARTLLIADEAAVPQIRL
QDSRQRVHSMTETHPNERLIITGFICANERGDTLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKLL
SSLSLAEANRLAHLGSPVLHNRTLQPLFDTQVSLAVRSSYASHTDFTLIAPKSSSASAPVVTNLSSVALFNLELTTELKQ
LIELLEETGLSPLAYWELGQGRAEITVTLENQKQVLALLDINRDALGVSDIKITEDLGLVALVSADASLYRRGFARLLSR
NARPLFNDGMSLVTLVPQGQVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWVELFSGAKAALNKELEASVELVGLVSS
RNALICDEGIELDSWKQAFEEHATPWQYSHLFEELHDLECDEVIALDISASADLTLQYPEFFARGIHVVSANKLAGSGPL
TFYRELKQQLDNRRLFWRYNASCGAGLPVQHALNDLHNSGDSIEAVGGIFSGTLCWLFENFDAKKPFSELVIEARGLGIT
EPDPRDDLSGRDMQRKLLILAREIGFEIELEDIELKSLVPANLAEIPLEQFLSRITELDDEMQQQFIAAAEQDKVLRYVA
SLDKVDGELKAEVGIQWVDISHPYANLTPGDNVFVIRSEFYQENPLIIRGPGAGRLVTAAAVQSDLAHICRDLLQE

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0460

COG function: function code E; Homoserine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the homoserine dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1790376, Length=814, Percent_Identity=41.1547911547912, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1786183, Length=827, Percent_Identity=29.3833131801693, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1790455, Length=298, Percent_Identity=32.5503355704698, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6322599, Length=346, Percent_Identity=32.9479768786127, Blast_Score=161, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6320893, Length=311, Percent_Identity=22.508038585209, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR005106
- InterPro:   IPR001341
- InterPro:   IPR018042
- InterPro:   IPR011147
- InterPro:   IPR001342
- InterPro:   IPR019811
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00696 AA_kinase; PF00742 Homoserine_dh; PF03447 NAD_binding_3 [H]

EC number: =2.7.2.4; =1.1.1.3 [H]

Molecular weight: Translated: 88075; Mature: 87944

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS01042 HOMOSER_DHGENASE ; PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYKLLELSTTKQ
CCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
LWQEELQTLISFQQNLIEQLLSNEQARSLRERLSNDKAQLISLLSIESINEYQSNQVVSF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEH
GERWSARLLAALLRESGVAASDVDARTLLIADEAAVPQIRLQDSRQRVHSMTETHPNERL
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCEE
IITGFICANERGDTLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKL
EEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHH
LSSLSLAEANRLAHLGSPVLHNRTLQPLFDTQVSLAVRSSYASHTDFTLIAPKSSSASAP
HHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
VVTNLSSVALFNLELTTELKQLIELLEETGLSPLAYWELGQGRAEITVTLENQKQVLALL
CCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCEEEEEE
DINRDALGVSDIKITEDLGLVALVSADASLYRRGFARLLSRNARPLFNDGMSLVTLVPQG
ECCCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHCCCEEEEEECCC
QVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWVELFSGAKAALNKELEASVELVGLVS
CHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHCHHHHCCEEEEEEEC
SRNALICDEGIELDSWKQAFEEHATPWQYSHLFEELHDLECDEVIALDISASADLTLQYP
CCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECH
EFFARGIHVVSANKLAGSGPLTFYRELKQQLDNRRLFWRYNASCGAGLPVQHALNDLHNS
HHHHCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCC
GDSIEAVGGIFSGTLCWLFENFDAKKPFSELVIEARGLGITEPDPRDDLSGRDMQRKLLI
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LAREIGFEIELEDIELKSLVPANLAEIPLEQFLSRITELDDEMQQQFIAAAEQDKVLRYV
HHHHCCCEEEEECEEHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLDKVDGELKAEVGIQWVDISHPYANLTPGDNVFVIRSEFYQENPLIIRGPGAGRLVTA
HHHHHCCCCEEHCCCEEEEECCCCCCCCCCCCCEEEEEHHCCCCCCEEEECCCCCCCHHH
AAVQSDLAHICRDLLQE
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARCHLHKFGGSSLADADCYRRVAHILLTHGHSDDLVVVSAAGKSTNFLYKLLELSTTKQ
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHH
LWQEELQTLISFQQNLIEQLLSNEQARSLRERLSNDKAQLISLLSIESINEYQSNQVVSF
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEH
GERWSARLLAALLRESGVAASDVDARTLLIADEAAVPQIRLQDSRQRVHSMTETHPNERL
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCEE
IITGFICANERGDTLLLGRNGSDFSATLIASLADIERVTIWTDVEGVFNADPNKINDAKL
EEEEEEEECCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCHHHH
LSSLSLAEANRLAHLGSPVLHNRTLQPLFDTQVSLAVRSSYASHTDFTLIAPKSSSASAP
HHHHHHHHHHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCC
VVTNLSSVALFNLELTTELKQLIELLEETGLSPLAYWELGQGRAEITVTLENQKQVLALL
CCCCCCEEEEEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEECCCCEEEEEE
DINRDALGVSDIKITEDLGLVALVSADASLYRRGFARLLSRNARPLFNDGMSLVTLVPQG
ECCCCCCCCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHCCCCCCHHCCCEEEEEECCC
QVNLLTQKVHRRCAGPRKRIGVLLLGVGNIGEAWVELFSGAKAALNKELEASVELVGLVS
CHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHCHHHHCCEEEEEEEC
SRNALICDEGIELDSWKQAFEEHATPWQYSHLFEELHDLECDEVIALDISASADLTLQYP
CCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCEEEECH
EFFARGIHVVSANKLAGSGPLTFYRELKQQLDNRRLFWRYNASCGAGLPVQHALNDLHNS
HHHHCCEEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHCC
GDSIEAVGGIFSGTLCWLFENFDAKKPFSELVIEARGLGITEPDPRDDLSGRDMQRKLLI
CCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHH
LAREIGFEIELEDIELKSLVPANLAEIPLEQFLSRITELDDEMQQQFIAAAEQDKVLRYV
HHHHCCCEEEEECEEHHHCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ASLDKVDGELKAEVGIQWVDISHPYANLTPGDNVFVIRSEFYQENPLIIRGPGAGRLVTA
HHHHHCCCCEEHCCCEEEEECCCCCCCCCCCCCEEEEEHHCCCCCCEEEECCCCCCCHHH
AAVQSDLAHICRDLLQE
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6298218; 8346018; 9278503 [H]