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The map label for this gene is dapF [H]

Identifier: 157960207

GI number: 157960207

Start: 457970

End: 458806

Strand: Direct

Name: dapF [H]

Synonym: Spea_0378

Alternate gene names: 157960207

Gene position: 457970-458806 (Clockwise)

Preceding gene: 157960206

Following gene: 157960208

Centisome position: 8.85

GC content: 49.82

Gene sequence:

>837_bases
ATGTCCATCTTGATCCAATTCACTAAGATGCATGGGCTAGGCAATGACTTTATGGTCGTCGATGGGGTGACACAGAATGT
GTTTTTCTCTCCCGATCAAATTAAGCGCTTGGCCGATCGGAATTTTGGCGTGGGCTTCGATCAGTTACTGCTGGTTGAGC
CTCCCTACGATCCCGATTTAGATTTTCATTATCGCATCTTTAATGCTGACGGCAGTGAGGTTGAGCAGTGTGGCAATGGC
GCACGCTGTTTCGCACGTTTTGTGCGTAATAAAGGCTTAACCCATAAGCATAAAATTCGCGTCAGCACCTCTTCGGGCAA
GATAACCTTACGCCTTGAGCGTGATGGCAAGGTGACCGTTAATATGGGGATCCCAGCTCTAGAGCCAGGTAAGATCCCAT
TTACCGCTAAGAAGGTAGAGAAAACCTATTTGTTGATGGCATCCAGTGGCACCTATCTATGTGGCGCTGTTTCTATGGGC
AATCCTCATTGCGTGATAGAGGTAGAAGATGTAGCCGCGACTGATGTCGATACTATTGGCGGCGAGCTGACTAACCACGA
GCGTTTTCCGAAGGGCGTGAATGTCGGCTTTATGCAGGTGCTAAATTCTGGCCATATTAAGCTCAGAGTCTACGAGCGCG
GCGCGGCAGAAACCTTAGCCTGTGGTACAGGAGCCTGTGCTGCAGTCGTTGTTGGGATCTTGCAGGGTAAACTGGATAGA
AATGTGCAGGTAGACTTGCCAGGTGGCAGCCTAATGATCAACTGGGACGGTGAGGGTAAGCCGCTATGGATGACTGGGCC
TGCTGAGCATGTTTATGATGGGCAGATTGCGCAATGA

Upstream 100 bases:

>100_bases
AAAAGTCGCTCAATTATGGCAAGGCGAGCAGATCCTGCCGTAAACTAGAATAATACTAAGCCGCGGACGCAAGGCGTGCG
CGGCAGATACTTTTTTAAGG

Downstream 100 bases:

>100_bases
TAGATAGCTCTAAGTCAAACCTACCCTTTGATGAGATTCTGATCCGTGAATACCTGCTGGATAACCCTGACTTCTTTAGT
CGTTATCCAGAGCTATTGCT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MSILIQFTKMHGLGNDFMVVDGVTQNVFFSPDQIKRLADRNFGVGFDQLLLVEPPYDPDLDFHYRIFNADGSEVEQCGNG
ARCFARFVRNKGLTHKHKIRVSTSSGKITLRLERDGKVTVNMGIPALEPGKIPFTAKKVEKTYLLMASSGTYLCGAVSMG
NPHCVIEVEDVAATDVDTIGGELTNHERFPKGVNVGFMQVLNSGHIKLRVYERGAAETLACGTGACAAVVVGILQGKLDR
NVQVDLPGGSLMINWDGEGKPLWMTGPAEHVYDGQIAQ

Sequences:

>Translated_278_residues
MSILIQFTKMHGLGNDFMVVDGVTQNVFFSPDQIKRLADRNFGVGFDQLLLVEPPYDPDLDFHYRIFNADGSEVEQCGNG
ARCFARFVRNKGLTHKHKIRVSTSSGKITLRLERDGKVTVNMGIPALEPGKIPFTAKKVEKTYLLMASSGTYLCGAVSMG
NPHCVIEVEDVAATDVDTIGGELTNHERFPKGVNVGFMQVLNSGHIKLRVYERGAAETLACGTGACAAVVVGILQGKLDR
NVQVDLPGGSLMINWDGEGKPLWMTGPAEHVYDGQIAQ
>Mature_277_residues
SILIQFTKMHGLGNDFMVVDGVTQNVFFSPDQIKRLADRNFGVGFDQLLLVEPPYDPDLDFHYRIFNADGSEVEQCGNGA
RCFARFVRNKGLTHKHKIRVSTSSGKITLRLERDGKVTVNMGIPALEPGKIPFTAKKVEKTYLLMASSGTYLCGAVSMGN
PHCVIEVEDVAATDVDTIGGELTNHERFPKGVNVGFMQVLNSGHIKLRVYERGAAETLACGTGACAAVVVGILQGKLDRN
VQVDLPGGSLMINWDGEGKPLWMTGPAEHVYDGQIAQ

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=272, Percent_Identity=68.75, Blast_Score=394, Evalue=1e-111,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30270; Mature: 30139

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILIQFTKMHGLGNDFMVVDGVTQNVFFSPDQIKRLADRNFGVGFDQLLLVEPPYDPDL
CEEEEEEEECCCCCCCEEEEECCCCCEEECHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
DFHYRIFNADGSEVEQCGNGARCFARFVRNKGLTHKHKIRVSTSSGKITLRLERDGKVTV
CEEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEEECCCEEEE
NMGIPALEPGKIPFTAKKVEKTYLLMASSGTYLCGAVSMGNPHCVIEVEDVAATDVDTIG
EECCCCCCCCCCCEEHHCCCEEEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCHHHCC
GELTNHERFPKGVNVGFMQVLNSGHIKLRVYERGAAETLACGTGACAAVVVGILQGKLDR
CCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHCCCCC
NVQVDLPGGSLMINWDGEGKPLWMTGPAEHVYDGQIAQ
CEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SILIQFTKMHGLGNDFMVVDGVTQNVFFSPDQIKRLADRNFGVGFDQLLLVEPPYDPDL
EEEEEEEECCCCCCCEEEEECCCCCEEECHHHHHHHHCCCCCCCCCEEEEECCCCCCCC
DFHYRIFNADGSEVEQCGNGARCFARFVRNKGLTHKHKIRVSTSSGKITLRLERDGKVTV
CEEEEEECCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEEEEEECCCEEEEEEECCCEEEE
NMGIPALEPGKIPFTAKKVEKTYLLMASSGTYLCGAVSMGNPHCVIEVEDVAATDVDTIG
EECCCCCCCCCCCEEHHCCCEEEEEEECCCCEEEEEEECCCCEEEEEECCCCCCCHHHCC
GELTNHERFPKGVNVGFMQVLNSGHIKLRVYERGAAETLACGTGACAAVVVGILQGKLDR
CCCCCCCCCCCCCCHHHHHHHCCCCEEEEEEECCCCCEEECCCCHHHHHHHHHHHCCCCC
NVQVDLPGGSLMINWDGEGKPLWMTGPAEHVYDGQIAQ
CEEEECCCCEEEEEECCCCCEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA