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The map label for this gene is hemD [H]

Identifier: 157960201

GI number: 157960201

Start: 451380

End: 452111

Strand: Reverse

Name: hemD [H]

Synonym: Spea_0372

Alternate gene names: 157960201

Gene position: 452111-451380 (Counterclockwise)

Preceding gene: 157960202

Following gene: 157960200

Centisome position: 8.74

GC content: 46.58

Gene sequence:

>732_bases
ATGAAAATCTTACTGACGCGCCCTGAAGGGCGCAATCAGTTGATGAAAGAGGCGTTGACTCAGCGCAACGTCTCTTACTT
CATCACGCCCCTACTCAACGTTCAGCCCACTTCTAATCTTGATGCTAATCAAATCGATAAGCAGCTTAAGCTAGCCGACA
TTATCATTTTCATCAGCACCAATGCCGTTGCTTTTGCCTCCCAAGCAATCGATAACCAATGGCCGAGTTCAGCGCAATAC
TTCGCCGTTGGCGATGCCACCCATGCCGCGTTGAAACAGCTCGGTATTGATGCCCAAAGAGCACCAGAAGATTGCCAACA
AACTGAAGGCTTGCTTACCCTGACCTCATTAGCCGATGTCACTGGCAAACAGATTATTATCGTTAGAGGTCAAGGTGGTC
GGGAAGATCTAGCCACAGAGCTAATCAAGCGAAATGCTCAGCTCAGCTATTGGGAAGTCTATAAGCGCGGCTGCCCAGCG
CTTGATAGCGCTAACCTTTGTCAGCAATGGCGAAGCTTTGGTATAGACACTATTATCATTACCAGTGGCGAAATACTCGA
TAACCTCATTAAAACAGTACCAAAAGAGCTATTTGCTTGGCTGCAAACTTGTCATATTATAGTCCCTAGCCCCCGAGTAT
ATGAAAAGGCAAAGGCTTATAATCTTTTGCATGTCAGCAATGCGACAGCCGCTAACACCAATGCCATGTTAGCAGCGTTA
TCTTTAAACTAG

Upstream 100 bases:

>100_bases
TGCCGATGCAGAGAAGCTAGGCGTTGCACTAGCAGAAGAACTGCTAAGCAAAGGTGCTAAAACGATTCTTGACGCTGTTT
ACGCGAACGCGTAACACTCA

Downstream 100 bases:

>100_bases
TTCGCCGCTAACGTAAGTTCATACTCGCTTTTACTGCTGCATAGCTTTCAAGGACTGTTTAATGGAAACCAAGAACACTG
ACGCCAGCGCTTCAGAGCCA

Product: uroporphyrinogen III synthase HEM4

Products: NA

Alternate protein names: UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MKILLTRPEGRNQLMKEALTQRNVSYFITPLLNVQPTSNLDANQIDKQLKLADIIIFISTNAVAFASQAIDNQWPSSAQY
FAVGDATHAALKQLGIDAQRAPEDCQQTEGLLTLTSLADVTGKQIIIVRGQGGREDLATELIKRNAQLSYWEVYKRGCPA
LDSANLCQQWRSFGIDTIIITSGEILDNLIKTVPKELFAWLQTCHIIVPSPRVYEKAKAYNLLHVSNATAANTNAMLAAL
SLN

Sequences:

>Translated_243_residues
MKILLTRPEGRNQLMKEALTQRNVSYFITPLLNVQPTSNLDANQIDKQLKLADIIIFISTNAVAFASQAIDNQWPSSAQY
FAVGDATHAALKQLGIDAQRAPEDCQQTEGLLTLTSLADVTGKQIIIVRGQGGREDLATELIKRNAQLSYWEVYKRGCPA
LDSANLCQQWRSFGIDTIIITSGEILDNLIKTVPKELFAWLQTCHIIVPSPRVYEKAKAYNLLHVSNATAANTNAMLAAL
SLN
>Mature_243_residues
MKILLTRPEGRNQLMKEALTQRNVSYFITPLLNVQPTSNLDANQIDKQLKLADIIIFISTNAVAFASQAIDNQWPSSAQY
FAVGDATHAALKQLGIDAQRAPEDCQQTEGLLTLTSLADVTGKQIIIVRGQGGREDLATELIKRNAQLSYWEVYKRGCPA
LDSANLCQQWRSFGIDTIIITSGEILDNLIKTVPKELFAWLQTCHIIVPSPRVYEKAKAYNLLHVSNATAANTNAMLAAL
SLN

Specific function: Catalyzes cyclization of the linear tetrapyrrole, hydroxymethylbilane, to the macrocyclic uroporphyrinogen III [H]

COG id: COG1587

COG function: function code H; Uroporphyrinogen-III synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the uroporphyrinogen-III synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790236, Length=247, Percent_Identity=31.5789473684211, Blast_Score=116, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003754 [H]

Pfam domain/function: PF02602 HEM4 [H]

EC number: =4.2.1.75 [H]

Molecular weight: Translated: 26811; Mature: 26811

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILLTRPEGRNQLMKEALTQRNVSYFITPLLNVQPTSNLDANQIDKQLKLADIIIFIST
CEEEEECCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHEEEEEEEEEEC
NAVAFASQAIDNQWPSSAQYFAVGDATHAALKQLGIDAQRAPEDCQQTEGLLTLTSLADV
CHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCHHHHHHCCEEEEHHHHHC
TGKQIIIVRGQGGREDLATELIKRNAQLSYWEVYKRGCPALDSANLCQQWRSFGIDTIII
CCCEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEE
TSGEILDNLIKTVPKELFAWLQTCHIIVPSPRVYEKAKAYNLLHVSNATAANTNAMLAAL
ECHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHEEEEECCCCCCCCCEEEEEE
SLN
ECC
>Mature Secondary Structure
MKILLTRPEGRNQLMKEALTQRNVSYFITPLLNVQPTSNLDANQIDKQLKLADIIIFIST
CEEEEECCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHEEEEEEEEEEC
NAVAFASQAIDNQWPSSAQYFAVGDATHAALKQLGIDAQRAPEDCQQTEGLLTLTSLADV
CHHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCHHHHHHCCEEEEHHHHHC
TGKQIIIVRGQGGREDLATELIKRNAQLSYWEVYKRGCPALDSANLCQQWRSFGIDTIII
CCCEEEEEECCCCHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCEEEE
TSGEILDNLIKTVPKELFAWLQTCHIIVPSPRVYEKAKAYNLLHVSNATAANTNAMLAAL
ECHHHHHHHHHHHHHHHHHHHHHHEEECCCCHHHHHHHHHEEEEECCCCCCCCCEEEEEE
SLN
ECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]