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The map label for this gene is hemX [H]

Identifier: 157960200

GI number: 157960200

Start: 450125

End: 451318

Strand: Reverse

Name: hemX [H]

Synonym: Spea_0371

Alternate gene names: 157960200

Gene position: 451318-450125 (Counterclockwise)

Preceding gene: 157960201

Following gene: 157960199

Centisome position: 8.72

GC content: 45.64

Gene sequence:

>1194_bases
ATGGAAACCAAGAACACTGACGCCAGCGCTTCAGAGCCAACAACTGAGCAAACTGTGGCGCAGCCCGTTATCGACACTCA
GTCAGAATCCCAAAGTCTGCCTAATGAAAGCACTCACTCCAATGAAAGCACTAGCTCTAATGGAAGTACTGTGCAAACAC
AGCTTACTCAAAGCAAGCCTGCGCCTTGGGGGATTATCTTTACCCTATTCTTATTACTGTTACTGACTTTTGCAGCAACG
GCTGGCGGATACTACTTCTATCAACAACTGCAGGCACAGCAAGCCGAGACTGCTGAGCTACAGCAAACCCTAGAGCAAAA
ACTACAAACGGTTCTCGTTGAACCAAATCAGCGTATTGCCTCTCTAGAGCAGCAGCAAAACCAATTTAAGTCTAGCGTCG
ACTTAACTCTGGCTCAGACACTCGATCAGCAAACACAGCTTGAAGAGCGTGTTTCTATTATTGCCCAGCGTAACCCTAAT
CACTGGCGCGCAGAAGAAGCTAAGTACTTAGTAAGACTGGCGGGACAGAAACTCTGGCTAGAGAAAGATCCAAGTACTGC
GACCAGCCTATTAAGAGCGGCCGATGAGCGTATTAAGTCGATGCGCGATCCCTCTTTGATGCCACTACGAAAGGCCCTAT
CAAAAGATATCGCCGCTGTAGCTACAGTTAAAAACACAGATATATCAGGTACCGTGTTGACCTTGGATAGCATCATTGAT
GGTTTAGAAAAGCTACCTTTAAACCGAGCAGAGACTCACTTCTCAGCCGATGAGCTCGCCGATACTGAAGTCAGCAACTC
ACTCGATGATTGGCAATCAAATCTAGCAAAATCTTGGCAAGCACTGATTGATGATTTCGTTATTGTTAGAAAACGCACCA
CGGATGCAGCGCCCCTACTTGAGCCCGACCAACAGTGGTATTTAGTCGAGAATACTCGCAATAAACTGCTGCAAGCCCAG
CTTGCATTGTATCGTCAAGATCAGGTTAATTACCGCAACTCAATTAAGCTAGCGAGAAAATGGATTTTTCAATACTTCGA
TCTGAAAGATCAACTAACCATTGATACGCTTGCCACCTTAGATGCTTTAGAGACACTGAAGATCCAAACGACCAGTATTG
AACGCTTCGAAGCATCTAATTTACTTAATCAGTTAGTCACTTACGGCAACTTGATGACAGAGGAGGCGCAGTAA

Upstream 100 bases:

>100_bases
AACACCAATGCCATGTTAGCAGCGTTATCTTTAAACTAGTTCGCCGCTAACGTAAGTTCATACTCGCTTTTACTGCTGCA
TAGCTTTCAAGGACTGTTTA

Downstream 100 bases:

>100_bases
TGGTCAGAGCGCTCGTCTATCTTGTCATTATCTTATTAGGACTGTGCATCAGTCCTTTTCTTGTGGGCAGCAAAGGCTAC
CTTTATCTAGCGATTGGCGA

Product: hypothetical protein

Products: NA

Alternate protein names: Urogen-III methylase; ORF X [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
METKNTDASASEPTTEQTVAQPVIDTQSESQSLPNESTHSNESTSSNGSTVQTQLTQSKPAPWGIIFTLFLLLLLTFAAT
AGGYYFYQQLQAQQAETAELQQTLEQKLQTVLVEPNQRIASLEQQQNQFKSSVDLTLAQTLDQQTQLEERVSIIAQRNPN
HWRAEEAKYLVRLAGQKLWLEKDPSTATSLLRAADERIKSMRDPSLMPLRKALSKDIAAVATVKNTDISGTVLTLDSIID
GLEKLPLNRAETHFSADELADTEVSNSLDDWQSNLAKSWQALIDDFVIVRKRTTDAAPLLEPDQQWYLVENTRNKLLQAQ
LALYRQDQVNYRNSIKLARKWIFQYFDLKDQLTIDTLATLDALETLKIQTTSIERFEASNLLNQLVTYGNLMTEEAQ

Sequences:

>Translated_397_residues
METKNTDASASEPTTEQTVAQPVIDTQSESQSLPNESTHSNESTSSNGSTVQTQLTQSKPAPWGIIFTLFLLLLLTFAAT
AGGYYFYQQLQAQQAETAELQQTLEQKLQTVLVEPNQRIASLEQQQNQFKSSVDLTLAQTLDQQTQLEERVSIIAQRNPN
HWRAEEAKYLVRLAGQKLWLEKDPSTATSLLRAADERIKSMRDPSLMPLRKALSKDIAAVATVKNTDISGTVLTLDSIID
GLEKLPLNRAETHFSADELADTEVSNSLDDWQSNLAKSWQALIDDFVIVRKRTTDAAPLLEPDQQWYLVENTRNKLLQAQ
LALYRQDQVNYRNSIKLARKWIFQYFDLKDQLTIDTLATLDALETLKIQTTSIERFEASNLLNQLVTYGNLMTEEAQ
>Mature_397_residues
METKNTDASASEPTTEQTVAQPVIDTQSESQSLPNESTHSNESTSSNGSTVQTQLTQSKPAPWGIIFTLFLLLLLTFAAT
AGGYYFYQQLQAQQAETAELQQTLEQKLQTVLVEPNQRIASLEQQQNQFKSSVDLTLAQTLDQQTQLEERVSIIAQRNPN
HWRAEEAKYLVRLAGQKLWLEKDPSTATSLLRAADERIKSMRDPSLMPLRKALSKDIAAVATVKNTDISGTVLTLDSIID
GLEKLPLNRAETHFSADELADTEVSNSLDDWQSNLAKSWQALIDDFVIVRKRTTDAAPLLEPDQQWYLVENTRNKLLQAQ
LALYRQDQVNYRNSIKLARKWIFQYFDLKDQLTIDTLATLDALETLKIQTTSIERFEASNLLNQLVTYGNLMTEEAQ

Specific function: Siroheme and cobalamin biosynthesis. [C]

COG id: COG2959

COG function: function code H; Uncharacterized enzyme of heme biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790235, Length=296, Percent_Identity=32.4324324324324, Blast_Score=158, Evalue=7e-40,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007470 [H]

Pfam domain/function: PF04375 HemX [H]

EC number: =2.1.1.107 [H]

Molecular weight: Translated: 44784; Mature: 44784

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METKNTDASASEPTTEQTVAQPVIDTQSESQSLPNESTHSNESTSSNGSTVQTQLTQSKP
CCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEHHCCCC
APWGIIFTLFLLLLLTFAATAGGYYFYQQLQAQQAETAELQQTLEQKLQTVLVEPNQRIA
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SLEQQQNQFKSSVDLTLAQTLDQQTQLEERVSIIAQRNPNHWRAEEAKYLVRLAGQKLWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHEE
EKDPSTATSLLRAADERIKSMRDPSLMPLRKALSKDIAAVATVKNTDISGTVLTLDSIID
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCCCEEEHHHHHH
GLEKLPLNRAETHFSADELADTEVSNSLDDWQSNLAKSWQALIDDFVIVRKRTTDAAPLL
HHHHCCCCHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EPDQQWYLVENTRNKLLQAQLALYRQDQVNYRNSIKLARKWIFQYFDLKDQLTIDTLATL
CCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DALETLKIQTTSIERFEASNLLNQLVTYGNLMTEEAQ
HHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
METKNTDASASEPTTEQTVAQPVIDTQSESQSLPNESTHSNESTSSNGSTVQTQLTQSKP
CCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEHHCCCC
APWGIIFTLFLLLLLTFAATAGGYYFYQQLQAQQAETAELQQTLEQKLQTVLVEPNQRIA
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHH
SLEQQQNQFKSSVDLTLAQTLDQQTQLEERVSIIAQRNPNHWRAEEAKYLVRLAGQKLWL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHEE
EKDPSTATSLLRAADERIKSMRDPSLMPLRKALSKDIAAVATVKNTDISGTVLTLDSIID
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHEECCCCCCCCEEEHHHHHH
GLEKLPLNRAETHFSADELADTEVSNSLDDWQSNLAKSWQALIDDFVIVRKRTTDAAPLL
HHHHCCCCHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
EPDQQWYLVENTRNKLLQAQLALYRQDQVNYRNSIKLARKWIFQYFDLKDQLTIDTLATL
CCCCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
DALETLKIQTTSIERFEASNLLNQLVTYGNLMTEEAQ
HHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3062586; 3054815; 1379743; 9278503; 9298646 [H]