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The map label for this gene is qacE [H]

Identifier: 157960100

GI number: 157960100

Start: 304145

End: 304471

Strand: Direct

Name: qacE [H]

Synonym: Spea_0271

Alternate gene names: 157960100

Gene position: 304145-304471 (Clockwise)

Preceding gene: 157960099

Following gene: 157960104

Centisome position: 5.88

GC content: 45.26

Gene sequence:

>327_bases
ATGAGCTGGATTTTTCTTTTATTGGGTGTGGTTGCGGAAGTGTTATCTCATGTGGCGCTAAAAGCCACCGATGGTTTTAG
TAAGCCTTTACCTGCGGCCCTGGTTATTTTAGGTCACCTAACAGCTTTTATCTTTTTAGGCCAAGCAATGAAGGGCATGC
CAGTAGGTGTGGTGCATGCTTTATGGGCCGGTTTAGCGATTGTGTCAGTGACATTAATCTCAGCTCTATTTTATCGCGAA
CATTTGGATATGACGGCTTGGATTGGAATGGTACTGGTGGCTGCAGGTGTGATGATGATTAACTTCTCACAGGGGCATGC
TCATTAG

Upstream 100 bases:

>100_bases
TAATGGTGATTATCAATTCTTACTAGAGGGATATAATAGCCTCACTAATTTAAATATCTTTGCAAACATAGCGTTGCTGA
ACAAATATTAAGGTAAGAGC

Downstream 100 bases:

>100_bases
CGATAAGCTAATGAATCAAAAATAAAAAGCCCGCAAGCTTAGCGCTTGCGGGCTTTTATTTTTGATTCATCTAAGGGGTA
ATCTAGGAGCTAATGCAAAG

Product: small multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Quaternary ammonium determinant E [H]

Number of amino acids: Translated: 108; Mature: 107

Protein sequence:

>108_residues
MSWIFLLLGVVAEVLSHVALKATDGFSKPLPAALVILGHLTAFIFLGQAMKGMPVGVVHALWAGLAIVSVTLISALFYRE
HLDMTAWIGMVLVAAGVMMINFSQGHAH

Sequences:

>Translated_108_residues
MSWIFLLLGVVAEVLSHVALKATDGFSKPLPAALVILGHLTAFIFLGQAMKGMPVGVVHALWAGLAIVSVTLISALFYRE
HLDMTAWIGMVLVAAGVMMINFSQGHAH
>Mature_107_residues
SWIFLLLGVVAEVLSHVALKATDGFSKPLPAALVILGHLTAFIFLGQAMKGMPVGVVHALWAGLAIVSVTLISALFYREH
LDMTAWIGMVLVAAGVMMINFSQGHAH

Specific function: Multidrug exporter. Is implicated for the resistance to bacteriocidal quaternary ammonium compounds [H]

COG id: COG2076

COG function: function code P; Membrane transporters of cations and cationic drugs

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the small multidrug resistance (SMR) protein family [H]

Homologues:

Organism=Escherichia coli, GI1786755, Length=107, Percent_Identity=34.5794392523364, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1787883, Length=99, Percent_Identity=34.3434343434343, Blast_Score=61, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000390 [H]

Pfam domain/function: PF00893 Multi_Drug_Res [H]

EC number: NA

Molecular weight: Translated: 11510; Mature: 11379

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.5 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWIFLLLGVVAEVLSHVALKATDGFSKPLPAALVILGHLTAFIFLGQAMKGMPVGVVHA
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LWAGLAIVSVTLISALFYREHLDMTAWIGMVLVAAGVMMINFSQGHAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure 
SWIFLLLGVVAEVLSHVALKATDGFSKPLPAALVILGHLTAFIFLGQAMKGMPVGVVHA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
LWAGLAIVSVTLISALFYREHLDMTAWIGMVLVAAGVMMINFSQGHAH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8494372 [H]