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The map label for this gene is citA [H]

Identifier: 157826331

GI number: 157826331

Start: 1184905

End: 1185396

Strand: Reverse

Name: citA [H]

Synonym: A1C_06600

Alternate gene names: 157826331

Gene position: 1185396-1184905 (Counterclockwise)

Preceding gene: 157826332

Following gene: 157826330

Centisome position: 96.29

GC content: 32.93

Gene sequence:

>492_bases
ATGCAAATGCTTGGTTATACTAATCAGCAAAGCACAATTGTTAGTAGTATAGTATTAATCGTAATGATGACAGTATTTCC
TATATCTACGTATGTTTCGGATAAGGTTGGGAGGCGTCCTGTACTAATATGTTGGATTATCTTGTTAATTTTATCAGTCT
ATCCTATTTTTGTAGCCTTAGGGTCAATGAATTTTACTTTAGCTATAATATCTCAAGTAATATTTGCTGGAATTATCGCT
ATTTATATGGGACCCATTCCAAAAGTTTTAGTAGAGATATTCCCGACTAGTGTAAGATTTACCGGCATTGCACTTTCATA
CAACCTTGCAGCTGCTGTATTTGGTGGTGCTGCTCCAATGTTAGCAATGATATTAACGAAAGTGACCGGAGACAATTATG
CTATTGCATATTATTTAATTGCACTTGCATTATTGTCTTCTATTCTTCTAAAATTCTACAAAGAAACTTATAAAAAGAAT
TTGGTAAATTAG

Upstream 100 bases:

>100_bases
AAACATTAACTAAATCTCCAAAAGAACTCATTATCGCACTTGGTCTTTATATTACTGTAACTGCGCCTTTTTATACTTCT
ACGGTTTTATCGGTAATTTT

Downstream 100 bases:

>100_bases
GTTCTATGATGAGCGCAGTTTTTGAAATACCAAACGGTGAAAGCAAAGTTTTACTACATTGTTGTTGTGCTCCTTGCGTT
GGTCCTTTAATGGAAAAAAT

Product: proline/betaine transporter

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: Citrate carrier protein; Citrate transporter; Citrate utilization determinant; Citrate utilization protein A [H]

Number of amino acids: Translated: 163; Mature: 163

Protein sequence:

>163_residues
MQMLGYTNQQSTIVSSIVLIVMMTVFPISTYVSDKVGRRPVLICWIILLILSVYPIFVALGSMNFTLAIISQVIFAGIIA
IYMGPIPKVLVEIFPTSVRFTGIALSYNLAAAVFGGAAPMLAMILTKVTGDNYAIAYYLIALALLSSILLKFYKETYKKN
LVN

Sequences:

>Translated_163_residues
MQMLGYTNQQSTIVSSIVLIVMMTVFPISTYVSDKVGRRPVLICWIILLILSVYPIFVALGSMNFTLAIISQVIFAGIIA
IYMGPIPKVLVEIFPTSVRFTGIALSYNLAAAVFGGAAPMLAMILTKVTGDNYAIAYYLIALALLSSILLKFYKETYKKN
LVN
>Mature_163_residues
MQMLGYTNQQSTIVSSIVLIVMMTVFPISTYVSDKVGRRPVLICWIILLILSVYPIFVALGSMNFTLAIISQVIFAGIIA
IYMGPIPKVLVEIFPTSVRFTGIALSYNLAAAVFGGAAPMLAMILTKVTGDNYAIAYYLIALALLSSILLKFYKETYKKN
LVN

Specific function: Uptake of citrate across the boundary membrane with the concomitant transport of protons into the cell (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=142, Percent_Identity=33.0985915492958, Blast_Score=62, Evalue=1e-11,
Organism=Escherichia coli, GI1790550, Length=159, Percent_Identity=28.9308176100629, Blast_Score=60, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 17872; Mature: 17872

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMLGYTNQQSTIVSSIVLIVMMTVFPISTYVSDKVGRRPVLICWIILLILSVYPIFVAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GSMNFTLAIISQVIFAGIIAIYMGPIPKVLVEIFPTSVRFTGIALSYNLAAAVFGGAAPM
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHH
LAMILTKVTGDNYAIAYYLIALALLSSILLKFYKETYKKNLVN
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQMLGYTNQQSTIVSSIVLIVMMTVFPISTYVSDKVGRRPVLICWIILLILSVYPIFVAL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
GSMNFTLAIISQVIFAGIIAIYMGPIPKVLVEIFPTSVRFTGIALSYNLAAAVFGGAAPM
CCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHH
LAMILTKVTGDNYAIAYYLIALALLSSILLKFYKETYKKNLVN
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]