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The map label for this gene is proP [H]

Identifier: 157826332

GI number: 157826332

Start: 1185345

End: 1186151

Strand: Reverse

Name: proP [H]

Synonym: A1C_06605

Alternate gene names: 157826332

Gene position: 1186151-1185345 (Counterclockwise)

Preceding gene: 157826333

Following gene: 157826331

Centisome position: 96.35

GC content: 37.79

Gene sequence:

>807_bases
ATGAGAAAAGTAATAATACCGGGAATGATCGGTAATGCTGCTGAGTGGTTTGATTATGCGTTATATGCACAATTTGCTTA
TATTATAGGGCAGCATTTTTTTCCTGATTCTGAAATGCGTGATACATTAACGTTTGCTGTATTTGCTGCGGGTTTTATAG
TGCGTCCACTCGGAGGTATTATTTTCGGTAATATCGGAGATAGATTCGGTAGACGCACTGCTTTAGTTATTGGTATTATA
ACTATGACGGTGCCGACTACAGGAATAGGGCTACTCCCTAGTTATCACACTATTGGAATTGCTGCCCCCATCATTTTGAC
TATTATTAGGCTTATACAAGGTTTTTCTCTAGGAGGTGAGTTTAGCGGCTGTATTTCTTATATAGTAGAGCATGCTTCGT
TTGAGCAAAGAGGGATTGCAGGAAGTGCCTCGTTTGTTAGTATGTGTGGCGGTATGCTTCTTGGGCTACTAACTTCTGCC
GGTTTTTCTTATTTTATGCCTGCCGATATGCTATTTGAATGGGGTTGGAGAATACCTTTTATCGCAGGATTATTTATTGG
CTCTATAGGTCTATATATCAGAAAAAATTTAGCAGCAAGCCCTATTTATAAAAAAGCTAAAGAGAACGGCATGTTAACAC
GTTTTCCTTTGTGTGAAACATTAACTAAATCTCCAAAAGAACTCATTATCGCACTTGGTCTTTATATTACTGTAACTGCG
CCTTTTTATACTTCTACGGTTTTATCGGTAATTTTATGCAAATGCTTGGTTATACTAATCAGCAAAGCACAATTGTTAGT
AGTATAG

Upstream 100 bases:

>100_bases
GACATAAATGAGCTGTACAATGAAGCTATAAAGTTATTTTTACTTGAGAATGTACAATATTCCTTTTATTATCTTGTTTT
AAAATCAATAAAATAACTTA

Downstream 100 bases:

>100_bases
TATTAATCGTAATGATGACAGTATTTCCTATATCTACGTATGTTTCGGATAAGGTTGGGAGGCGTCCTGTACTAATATGT
TGGATTATCTTGTTAATTTT

Product: proline/betaine transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRKVIIPGMIGNAAEWFDYALYAQFAYIIGQHFFPDSEMRDTLTFAVFAAGFIVRPLGGIIFGNIGDRFGRRTALVIGII
TMTVPTTGIGLLPSYHTIGIAAPIILTIIRLIQGFSLGGEFSGCISYIVEHASFEQRGIAGSASFVSMCGGMLLGLLTSA
GFSYFMPADMLFEWGWRIPFIAGLFIGSIGLYIRKNLAASPIYKKAKENGMLTRFPLCETLTKSPKELIIALGLYITVTA
PFYTSTVLSVILCKCLVILISKAQLLVV

Sequences:

>Translated_268_residues
MRKVIIPGMIGNAAEWFDYALYAQFAYIIGQHFFPDSEMRDTLTFAVFAAGFIVRPLGGIIFGNIGDRFGRRTALVIGII
TMTVPTTGIGLLPSYHTIGIAAPIILTIIRLIQGFSLGGEFSGCISYIVEHASFEQRGIAGSASFVSMCGGMLLGLLTSA
GFSYFMPADMLFEWGWRIPFIAGLFIGSIGLYIRKNLAASPIYKKAKENGMLTRFPLCETLTKSPKELIIALGLYITVTA
PFYTSTVLSVILCKCLVILISKAQLLVV
>Mature_268_residues
MRKVIIPGMIGNAAEWFDYALYAQFAYIIGQHFFPDSEMRDTLTFAVFAAGFIVRPLGGIIFGNIGDRFGRRTALVIGII
TMTVPTTGIGLLPSYHTIGIAAPIILTIIRLIQGFSLGGEFSGCISYIVEHASFEQRGIAGSASFVSMCGGMLLGLLTSA
GFSYFMPADMLFEWGWRIPFIAGLFIGSIGLYIRKNLAASPIYKKAKENGMLTRFPLCETLTKSPKELIIALGLYITVTA
PFYTSTVLSVILCKCLVILISKAQLLVV

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=254, Percent_Identity=37.007874015748, Blast_Score=170, Evalue=7e-44,
Organism=Escherichia coli, GI1788292, Length=240, Percent_Identity=34.1666666666667, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789941, Length=257, Percent_Identity=35.408560311284, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1788942, Length=246, Percent_Identity=34.9593495934959, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI87082231, Length=224, Percent_Identity=27.2321428571429, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 29151; Mature: 29151

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKVIIPGMIGNAAEWFDYALYAQFAYIIGQHFFPDSEMRDTLTFAVFAAGFIVRPLGGI
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHE
IFGNIGDRFGRRTALVIGIITMTVPTTGIGLLPSYHTIGIAAPIILTIIRLIQGFSLGGE
EECCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
FSGCISYIVEHASFEQRGIAGSASFVSMCGGMLLGLLTSAGFSYFMPADMLFEWGWRIPF
HHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHCCCCHHH
IAGLFIGSIGLYIRKNLAASPIYKKAKENGMLTRFPLCETLTKSPKELIIALGLYITVTA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCHHHHHHCCHHHHHHHHHHHHEEEC
PFYTSTVLSVILCKCLVILISKAQLLVV
CHHHHHHHHHHHHHHHHHHHCCCHHEEC
>Mature Secondary Structure
MRKVIIPGMIGNAAEWFDYALYAQFAYIIGQHFFPDSEMRDTLTFAVFAAGFIVRPLGGI
CCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHE
IFGNIGDRFGRRTALVIGIITMTVPTTGIGLLPSYHTIGIAAPIILTIIRLIQGFSLGGE
EECCHHHHHCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCC
FSGCISYIVEHASFEQRGIAGSASFVSMCGGMLLGLLTSAGFSYFMPADMLFEWGWRIPF
HHHHHHHHHHHHCHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHCCHHHHHHCCCCHHH
IAGLFIGSIGLYIRKNLAASPIYKKAKENGMLTRFPLCETLTKSPKELIIALGLYITVTA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEECCHHHHHHCCHHHHHHHHHHHHEEEC
PFYTSTVLSVILCKCLVILISKAQLLVV
CHHHHHHHHHHHHHHHHHHHCCCHHEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]