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The map label for this gene is glyS [H]

Identifier: 157826327

GI number: 157826327

Start: 1180965

End: 1182953

Strand: Reverse

Name: glyS [H]

Synonym: A1C_06580

Alternate gene names: 157826327

Gene position: 1182953-1180965 (Counterclockwise)

Preceding gene: 157826328

Following gene: 157826326

Centisome position: 96.09

GC content: 31.52

Gene sequence:

>1989_bases
GTGAGTGAATTATTATTAGAGCTATTTAGTGAAGAAATACCTGCATTTATGCAAAAAAACGCTGAAGAGGGGTATTTAAA
TATATTCACACAAATTTTTGCAGAAAGCGCAATATTTGCAAAAGTACAAGTATTTGTAGGACCTCGTAGGATAACGCTGT
ATGCTACCCATTTGCCAAAGGTCACTTTGCCACAAGAGGAAGAAATTAAAGGACCAAGCATAGAATCTCCGGAAGCCGCT
ATTAACGGCTTTTGCAGTGCTCATAATGTTAGTAAATTAGAGCTTTCTACTAAATTAATTAACAATCAGCTATATTATTT
CTTTGTCAAAAAGACAAAAGAAAGAGAAATAAAAGAGATTTTGCCTAAGATTATTATAGAGGCTATTAATAAATATAGCT
GGGCAAAATCGATGTTTTGGGGTGATTACAAAATAAAATGGATCAGACCTTTGCGAAATATTTTATGTATATTTGATGGT
GAAGTACTACCACTGCAATTTGGGCATTTAACCGCTAATAATATTACGTACGGGCATCGTCTTACTGATAATAAAAAATT
AGAAGTAACAGATTTTAAAGATTATAGAAATAAGCTTTTAGAAAATAACGTTGTTTTAGAAAGAATAAAGAGAGAAGAAA
TAATTAAGACCGGCTTATTAGAGCTAGCAAATTCTCAAAATCTAAATATAAAAGAAGATGCGCGTTTAATAGAAGAAGTA
GCAGGACTTAGCGAATTTCCTGTCGTATTGCTTGGGAAAATAGCGCAGAAATTCTTAGAGCTACCTAAAGAAGTACTTAT
TGCTTCGATGCGTACGCATCAGAAATATTTTTGTTTATTTGACAAAACAGGAAATTTTGCACCATATTTTCTCTTTGTTA
GTAATGGTAGATTTGCAAATGCTGAACTCGTTATTCAAGGTAATGAAAAAGTACTATCGGCAAGACTTTCCGATGCTTTA
TATTTTTATAAACAAGATATAGCTAAAACTTTAGAATCAAGATTAGGTAAATTAGAAGCTGTAACATTTCATGCAAAACT
TGGTAACTTAAGAGAAAAAATTGAACACATAACCGATATTTGTAATTATATAGCTCCAAATAATAAAGATTTAATTACGG
CAGCAAAGCTTTGCAAAAGTGACCTTGTTTCTGAGATGGTTGGAGAATTTCCTGATCTGCAAGGTATTATGGGTTATTAT
TATGCAAAATATGAAGGGCTAAATGAAGAAATAGCCGCTGCAATTAGAGACCATTATAAACCGCAAGGTTTGAGTGACAA
TGTAGCAAGCGGAAATGCGGCATTGCTTGCTTTAGCCGATAAGCTAGATAGTCTAGTAGGTTTGATGATAGCAGGCGAAG
CTCCAACAGGTTCAGGTGACCCATATGCATTGAGACGTCAAGCTCTTGGTATAATAAGAATAATACTTGAAAATAAATTA
GAACTAAATTTAAATGATTTAATTATTTTTTCCTTAAAGTTATATGGAAATTCAGCAGATAAAGATTTAATAACATCTTT
CTTTGAAGAAAGAGCAAAATTTTACTTTAAAAATAAATATGATATTTTATTAATTAATGCTGTTCTTGATTTAAATTTAG
TAGATACACAATTTAAGCTTGAGACGCTAAAAGAATTTTTAATAGAAGACGCAGGCAAGCAGTTATTAAATGCTTATAAA
CGAGCGAGCAATATAATCGGTGATCAGAAAATTACCGGCCTAGTTGATGCTAGTCTCTTTAGTACACAGCCTGAAAAAGA
CTTATTTGAAGTAGTCCAAAAAATTTCTCCACAGATTATAGATAGTATAGCTGATAAAGATTATAAGAGAGCCTTAAATT
TATTATCGTTTCTATTAACTCCTATTACTAGCTTTTTTGATAATGTATTAGTTAATGATCCTGATCCAAAAATCGCTCAA
AATCGTTTATCGCTATTACAAAATATTTGCGAGTTATTAAATAAAATTGCTAAGTTTAACCGCTTATGA

Upstream 100 bases:

>100_bases
AATGCGTTAGGTGTAATTAGTGTAACGGAGCGTGCTTCTTATGTTTTAAGAGTGCGTCATTTAGCTAGAATTTGTTGTAT
GAAATGGTTGGAGTTAAGCG

Downstream 100 bases:

>100_bases
CTGAAACTTTCTTATGTAATTCACGCGACAGCGGGAATCCATTAAGCAGATATATAACAAAACCAAAATAACTAAATAAT
GGTTAGTAAAGCAGTTCAAT

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 662; Mature: 661

Protein sequence:

>662_residues
MSELLLELFSEEIPAFMQKNAEEGYLNIFTQIFAESAIFAKVQVFVGPRRITLYATHLPKVTLPQEEEIKGPSIESPEAA
INGFCSAHNVSKLELSTKLINNQLYYFFVKKTKEREIKEILPKIIIEAINKYSWAKSMFWGDYKIKWIRPLRNILCIFDG
EVLPLQFGHLTANNITYGHRLTDNKKLEVTDFKDYRNKLLENNVVLERIKREEIIKTGLLELANSQNLNIKEDARLIEEV
AGLSEFPVVLLGKIAQKFLELPKEVLIASMRTHQKYFCLFDKTGNFAPYFLFVSNGRFANAELVIQGNEKVLSARLSDAL
YFYKQDIAKTLESRLGKLEAVTFHAKLGNLREKIEHITDICNYIAPNNKDLITAAKLCKSDLVSEMVGEFPDLQGIMGYY
YAKYEGLNEEIAAAIRDHYKPQGLSDNVASGNAALLALADKLDSLVGLMIAGEAPTGSGDPYALRRQALGIIRIILENKL
ELNLNDLIIFSLKLYGNSADKDLITSFFEERAKFYFKNKYDILLINAVLDLNLVDTQFKLETLKEFLIEDAGKQLLNAYK
RASNIIGDQKITGLVDASLFSTQPEKDLFEVVQKISPQIIDSIADKDYKRALNLLSFLLTPITSFFDNVLVNDPDPKIAQ
NRLSLLQNICELLNKIAKFNRL

Sequences:

>Translated_662_residues
MSELLLELFSEEIPAFMQKNAEEGYLNIFTQIFAESAIFAKVQVFVGPRRITLYATHLPKVTLPQEEEIKGPSIESPEAA
INGFCSAHNVSKLELSTKLINNQLYYFFVKKTKEREIKEILPKIIIEAINKYSWAKSMFWGDYKIKWIRPLRNILCIFDG
EVLPLQFGHLTANNITYGHRLTDNKKLEVTDFKDYRNKLLENNVVLERIKREEIIKTGLLELANSQNLNIKEDARLIEEV
AGLSEFPVVLLGKIAQKFLELPKEVLIASMRTHQKYFCLFDKTGNFAPYFLFVSNGRFANAELVIQGNEKVLSARLSDAL
YFYKQDIAKTLESRLGKLEAVTFHAKLGNLREKIEHITDICNYIAPNNKDLITAAKLCKSDLVSEMVGEFPDLQGIMGYY
YAKYEGLNEEIAAAIRDHYKPQGLSDNVASGNAALLALADKLDSLVGLMIAGEAPTGSGDPYALRRQALGIIRIILENKL
ELNLNDLIIFSLKLYGNSADKDLITSFFEERAKFYFKNKYDILLINAVLDLNLVDTQFKLETLKEFLIEDAGKQLLNAYK
RASNIIGDQKITGLVDASLFSTQPEKDLFEVVQKISPQIIDSIADKDYKRALNLLSFLLTPITSFFDNVLVNDPDPKIAQ
NRLSLLQNICELLNKIAKFNRL
>Mature_661_residues
SELLLELFSEEIPAFMQKNAEEGYLNIFTQIFAESAIFAKVQVFVGPRRITLYATHLPKVTLPQEEEIKGPSIESPEAAI
NGFCSAHNVSKLELSTKLINNQLYYFFVKKTKEREIKEILPKIIIEAINKYSWAKSMFWGDYKIKWIRPLRNILCIFDGE
VLPLQFGHLTANNITYGHRLTDNKKLEVTDFKDYRNKLLENNVVLERIKREEIIKTGLLELANSQNLNIKEDARLIEEVA
GLSEFPVVLLGKIAQKFLELPKEVLIASMRTHQKYFCLFDKTGNFAPYFLFVSNGRFANAELVIQGNEKVLSARLSDALY
FYKQDIAKTLESRLGKLEAVTFHAKLGNLREKIEHITDICNYIAPNNKDLITAAKLCKSDLVSEMVGEFPDLQGIMGYYY
AKYEGLNEEIAAAIRDHYKPQGLSDNVASGNAALLALADKLDSLVGLMIAGEAPTGSGDPYALRRQALGIIRIILENKLE
LNLNDLIIFSLKLYGNSADKDLITSFFEERAKFYFKNKYDILLINAVLDLNLVDTQFKLETLKEFLIEDAGKQLLNAYKR
ASNIIGDQKITGLVDASLFSTQPEKDLFEVVQKISPQIIDSIADKDYKRALNLLSFLLTPITSFFDNVLVNDPDPKIAQN
RLSLLQNICELLNKIAKFNRL

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=688, Percent_Identity=30.8139534883721, Blast_Score=314, Evalue=9e-87,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75146; Mature: 75015

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELLLELFSEEIPAFMQKNAEEGYLNIFTQIFAESAIFAKVQVFVGPRRITLYATHLPK
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEEECCCC
VTLPQEEEIKGPSIESPEAAINGFCSAHNVSKLELSTKLINNQLYYFFVKKTKEREIKEI
CCCCCHHCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHH
LPKIIIEAINKYSWAKSMFWGDYKIKWIRPLRNILCIFDGEVLPLQFGHLTANNITYGHR
HHHHHHHHHHHHHHHHHHEECCEEEHHHHHHHHHEEHCCCCEEEEEECCEEECCEEECCE
LTDNKKLEVTDFKDYRNKLLENNVVLERIKREEIIKTGLLELANSQNLNIKEDARLIEEV
ECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AGLSEFPVVLLGKIAQKFLELPKEVLIASMRTHQKYFCLFDKTGNFAPYFLFVSNGRFAN
HCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCEEC
AELVIQGNEKVLSARLSDALYFYKQDIAKTLESRLGKLEAVTFHAKLGNLREKIEHITDI
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
CNYIAPNNKDLITAAKLCKSDLVSEMVGEFPDLQGIMGYYYAKYEGLNEEIAAAIRDHYK
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC
PQGLSDNVASGNAALLALADKLDSLVGLMIAGEAPTGSGDPYALRRQALGIIRIILENKL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCC
ELNLNDLIIFSLKLYGNSADKDLITSFFEERAKFYFKNKYDILLINAVLDLNLVDTQFKL
CCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEECHHHHH
ETLKEFLIEDAGKQLLNAYKRASNIIGDQKITGLVDASLFSTQPEKDLFEVVQKISPQII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHH
DSIADKDYKRALNLLSFLLTPITSFFDNVLVNDPDPKIAQNRLSLLQNICELLNKIAKFN
HHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
RL
CC
>Mature Secondary Structure 
SELLLELFSEEIPAFMQKNAEEGYLNIFTQIFAESAIFAKVQVFVGPRRITLYATHLPK
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEEEEEECCEEEEEEEECCCC
VTLPQEEEIKGPSIESPEAAINGFCSAHNVSKLELSTKLINNQLYYFFVKKTKEREIKEI
CCCCCHHCCCCCCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEEEECCHHHHHHHH
LPKIIIEAINKYSWAKSMFWGDYKIKWIRPLRNILCIFDGEVLPLQFGHLTANNITYGHR
HHHHHHHHHHHHHHHHHHEECCEEEHHHHHHHHHEEHCCCCEEEEEECCEEECCEEECCE
LTDNKKLEVTDFKDYRNKLLENNVVLERIKREEIIKTGLLELANSQNLNIKEDARLIEEV
ECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AGLSEFPVVLLGKIAQKFLELPKEVLIASMRTHQKYFCLFDKTGNFAPYFLFVSNGRFAN
HCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEEEEEEECCCCCCEEEEEEECCCEEC
AELVIQGNEKVLSARLSDALYFYKQDIAKTLESRLGKLEAVTFHAKLGNLREKIEHITDI
CEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHH
CNYIAPNNKDLITAAKLCKSDLVSEMVGEFPDLQGIMGYYYAKYEGLNEEIAAAIRDHYK
HHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCC
PQGLSDNVASGNAALLALADKLDSLVGLMIAGEAPTGSGDPYALRRQALGIIRIILENKL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHCCC
ELNLNDLIIFSLKLYGNSADKDLITSFFEERAKFYFKNKYDILLINAVLDLNLVDTQFKL
CCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCEEECHHHHH
ETLKEFLIEDAGKQLLNAYKRASNIIGDQKITGLVDASLFSTQPEKDLFEVVQKISPQII
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHH
DSIADKDYKRALNLLSFLLTPITSFFDNVLVNDPDPKIAQNRLSLLQNICELLNKIAKFN
HHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHC
RL
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA