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The map label for this gene is nusA [H]

Identifier: 157825875

GI number: 157825875

Start: 735212

End: 736723

Strand: Reverse

Name: nusA [H]

Synonym: A1C_04070

Alternate gene names: 157825875

Gene position: 736723-735212 (Counterclockwise)

Preceding gene: 157825876

Following gene: 157825874

Centisome position: 59.84

GC content: 33.47

Gene sequence:

>1512_bases
ATGTCTAATATAGGTAATGTAGAAATTTTACAAATTATAGATTCTGTAGCTCGTGAGAAAGGAATATCAAAAGAAATTTT
GGTTTCAACTGTTGAACAAGCGGTACAAGTAGCAGGACGAAAAAAATACGGTAATGAATATAATATTAAAGCTCAGATAA
ATAGGAAAACCGGTGAGATAAATCTTTTAAGAATCTTAAAAATAGTAGAAGATGTACAAGATTACTTAACGCAAATCTCA
CTTGCAGAAGCTTTAAGAAAGAATCCGGAAGCTAAAATCGGTGATGAGATATATGAATATTTACCACCGATTGATCATGC
GAGAGTATCAGCGCAAGCTGCAAAACAAGTGATAACTCAACGTGTTATAGAAGCTGAGCGTGAAAAACAATATCATGACT
TTAAAGATAGAAAAGGCGAAATTATAAACGGGATAGTTAAGAGAATAGAATACGGTGATATAATAGTTGACTTGAGTCGT
GCTGAAGCAATAATAAAAAAAGATCAATTAATTAATGGTGAGAATTTTAAACCTAATGATCGGATAAAAGCATATGTACA
GGATGTAAGGCAAGAGACAAAAGGACCGCAGATTTTCTTGTCTAGAGTAGATAACCAGATGCTAGTTAAACTATTTAAGA
TAGAAGTACCTGAAATTCTTGAAGATATTATTCAAATAAAATCAGTAGCACGTGATCCCGGTTCAAGAGCAAAAATAGCG
GTATTTGCTTCGGATAGTAGTATAGATCCCGTAGGCTCATGTGTAGGTATTAGAGGTAATAGAGTAAAAGCAGTAACAAA
TGAGTTAAACGGAGAGAAAATTGATATAGTATTATGGAGTAATGATCTTGCACAGTTTATAGTTAATGCTCTTGCACCGC
TTGCACCTGGGGAGATTACAAAAATTTTGATTGATGAGGATAGGCATAAGGTAGAAGTAGTAGTTTCACAAGAAAATCAA
AGTATTGCAATAGGTAGAAGAGGGCAGAATGTTCGTTTAGCTTCTAAGCTTACAGGTTGGAATATCGATATAATGACTGA
AGAGCAGGAATCAAAAAGAAGAAACGAAGAGTTTGTGACTTCTACGCAATTATTTATGGAAGCTTTAGATGTTGAAGAAG
TTATAGGTCAGCTTTTATCGGTAACGGGATTTAATTCGGTAGAACAAATTGCCAGTAGTGAGGTTAGTACTTTGACAAGA
ATCGAAGGTTTTGAGGAAGAACTTGCAGTAGAGATCAAAAATCGAGCTGTTAATTACGTCGACCTTAAGAATGAAAAGAT
TATCAAAAAGTTAGAGGATTTAGGAGTTGAGCAAGAATTAATAGATATATTAGCATTACCGCTTGAATTAATATTAAAAT
TTGCCGAATATGGTATAAAGACCATAGAAGATTTAGGAGAGATGAGTGTAAATGAATTTAAAAATTTAGCTCCAAATTCT
AATATAACGGACGAAAATATTAAATTACTGATTAAAACTGCTAGACGGCACGGTGAATTAAAGGATAGTTAG

Upstream 100 bases:

>100_bases
TTTCTAATTGATTATCTATTTTGAGGTATTTTTAAAAAATAACCAAAATTAAATCGTGTAAGAAATTGTCGCAATACCCT
AAAGCAATAAGAGATAATGT

Downstream 100 bases:

>100_bases
AGGATGGGAATGTACGTCATTCCTGCGAAATGGTGGTTGTATGAATCAAAAAACACTCTCGATGTTATTCCCGCGTAGGC
GTGAATCCAGCATAAAGTGA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MSNIGNVEILQIIDSVAREKGISKEILVSTVEQAVQVAGRKKYGNEYNIKAQINRKTGEINLLRILKIVEDVQDYLTQIS
LAEALRKNPEAKIGDEIYEYLPPIDHARVSAQAAKQVITQRVIEAEREKQYHDFKDRKGEIINGIVKRIEYGDIIVDLSR
AEAIIKKDQLINGENFKPNDRIKAYVQDVRQETKGPQIFLSRVDNQMLVKLFKIEVPEILEDIIQIKSVARDPGSRAKIA
VFASDSSIDPVGSCVGIRGNRVKAVTNELNGEKIDIVLWSNDLAQFIVNALAPLAPGEITKILIDEDRHKVEVVVSQENQ
SIAIGRRGQNVRLASKLTGWNIDIMTEEQESKRRNEEFVTSTQLFMEALDVEEVIGQLLSVTGFNSVEQIASSEVSTLTR
IEGFEEELAVEIKNRAVNYVDLKNEKIIKKLEDLGVEQELIDILALPLELILKFAEYGIKTIEDLGEMSVNEFKNLAPNS
NITDENIKLLIKTARRHGELKDS

Sequences:

>Translated_503_residues
MSNIGNVEILQIIDSVAREKGISKEILVSTVEQAVQVAGRKKYGNEYNIKAQINRKTGEINLLRILKIVEDVQDYLTQIS
LAEALRKNPEAKIGDEIYEYLPPIDHARVSAQAAKQVITQRVIEAEREKQYHDFKDRKGEIINGIVKRIEYGDIIVDLSR
AEAIIKKDQLINGENFKPNDRIKAYVQDVRQETKGPQIFLSRVDNQMLVKLFKIEVPEILEDIIQIKSVARDPGSRAKIA
VFASDSSIDPVGSCVGIRGNRVKAVTNELNGEKIDIVLWSNDLAQFIVNALAPLAPGEITKILIDEDRHKVEVVVSQENQ
SIAIGRRGQNVRLASKLTGWNIDIMTEEQESKRRNEEFVTSTQLFMEALDVEEVIGQLLSVTGFNSVEQIASSEVSTLTR
IEGFEEELAVEIKNRAVNYVDLKNEKIIKKLEDLGVEQELIDILALPLELILKFAEYGIKTIEDLGEMSVNEFKNLAPNS
NITDENIKLLIKTARRHGELKDS
>Mature_502_residues
SNIGNVEILQIIDSVAREKGISKEILVSTVEQAVQVAGRKKYGNEYNIKAQINRKTGEINLLRILKIVEDVQDYLTQISL
AEALRKNPEAKIGDEIYEYLPPIDHARVSAQAAKQVITQRVIEAEREKQYHDFKDRKGEIINGIVKRIEYGDIIVDLSRA
EAIIKKDQLINGENFKPNDRIKAYVQDVRQETKGPQIFLSRVDNQMLVKLFKIEVPEILEDIIQIKSVARDPGSRAKIAV
FASDSSIDPVGSCVGIRGNRVKAVTNELNGEKIDIVLWSNDLAQFIVNALAPLAPGEITKILIDEDRHKVEVVVSQENQS
IAIGRRGQNVRLASKLTGWNIDIMTEEQESKRRNEEFVTSTQLFMEALDVEEVIGQLLSVTGFNSVEQIASSEVSTLTRI
EGFEEELAVEIKNRAVNYVDLKNEKIIKKLEDLGVEQELIDILALPLELILKFAEYGIKTIEDLGEMSVNEFKNLAPNSN
ITDENIKLLIKTARRHGELKDS

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=491, Percent_Identity=38.6965376782077, Blast_Score=359, Evalue=1e-100,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 56632; Mature: 56500

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIGNVEILQIIDSVAREKGISKEILVSTVEQAVQVAGRKKYGNEYNIKAQINRKTGEI
CCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCH
NLLRILKIVEDVQDYLTQISLAEALRKNPEAKIGDEIYEYLPPIDHARVSAQAAKQVITQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RVIEAEREKQYHDFKDRKGEIINGIVKRIEYGDIIVDLSRAEAIIKKDQLINGENFKPND
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCCCCH
RIKAYVQDVRQETKGPQIFLSRVDNQMLVKLFKIEVPEILEDIIQIKSVARDPGSRAKIA
HHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEE
VFASDSSIDPVGSCVGIRGNRVKAVTNELNGEKIDIVLWSNDLAQFIVNALAPLAPGEIT
EEECCCCCCCCHHEECCCCCEEHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEE
KILIDEDRHKVEVVVSQENQSIAIGRRGQNVRLASKLTGWNIDIMTEEQESKRRNEEFVT
EEEEECCCCEEEEEEECCCCEEEEECCCCCEEEEHHCCCCEEEEEECCHHHHHHHHHHHH
STQLFMEALDVEEVIGQLLSVTGFNSVEQIASSEVSTLTRIEGFEEELAVEIKNRAVNYV
HHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEE
DLKNEKIIKKLEDLGVEQELIDILALPLELILKFAEYGIKTIEDLGEMSVNEFKNLAPNS
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NITDENIKLLIKTARRHGELKDS
CCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SNIGNVEILQIIDSVAREKGISKEILVSTVEQAVQVAGRKKYGNEYNIKAQINRKTGEI
CCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCH
NLLRILKIVEDVQDYLTQISLAEALRKNPEAKIGDEIYEYLPPIDHARVSAQAAKQVITQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RVIEAEREKQYHDFKDRKGEIINGIVKRIEYGDIIVDLSRAEAIIKKDQLINGENFKPND
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHCCCCCCCCCCH
RIKAYVQDVRQETKGPQIFLSRVDNQMLVKLFKIEVPEILEDIIQIKSVARDPGSRAKIA
HHHHHHHHHHHHCCCHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEE
VFASDSSIDPVGSCVGIRGNRVKAVTNELNGEKIDIVLWSNDLAQFIVNALAPLAPGEIT
EEECCCCCCCCHHEECCCCCEEHHHHHCCCCCEEEEEEECCHHHHHHHHHHCCCCCCCEE
KILIDEDRHKVEVVVSQENQSIAIGRRGQNVRLASKLTGWNIDIMTEEQESKRRNEEFVT
EEEEECCCCEEEEEEECCCCEEEEECCCCCEEEEHHCCCCEEEEEECCHHHHHHHHHHHH
STQLFMEALDVEEVIGQLLSVTGFNSVEQIASSEVSTLTRIEGFEEELAVEIKNRAVNYV
HHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCEE
DLKNEKIIKKLEDLGVEQELIDILALPLELILKFAEYGIKTIEDLGEMSVNEFKNLAPNS
ECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NITDENIKLLIKTARRHGELKDS
CCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]