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The map label for this gene is hsrA [H]

Identifier: 157373126

GI number: 157373126

Start: 5431051

End: 5432466

Strand: Direct

Name: hsrA [H]

Synonym: Spro_4895

Alternate gene names: 157373126

Gene position: 5431051-5432466 (Clockwise)

Preceding gene: 157373125

Following gene: 157373134

Centisome position: 99.67

GC content: 57.56

Gene sequence:

>1416_bases
ATGACCAAATCCGCGCGCAGTATGGCAGGACTACCCTGGATCGCCGCCATGGCGTTCTTTATGCAGGCACTGGATGCCAC
CATCCTCAATACGGCGTTGCCAGCGATAGCTCAAAGTCTTGAGCGCTCGCCGTTGGCGATGCAGTCGGCGGTTATCAGCT
ATACCCTGACGGTAGCGATGCTGATCCCGGTCAGCGGCTGGCTGGCGGATCGCTTCGGTACCCGCCGGGTGTTTATCATC
GCCGTTTCTCTGTTTACTCTCGGTTCGCTGCTGTGTGCGCTTTCTCCGACGTTAAGCCTGTTGGTAACTTCACGAGTCAT
TCAGGGTGTCGGCGGAGCGATGATGATGCCGGTGGCGCGCCTGGCTCTGCTGCGCGCCTACCCACGCAGCGAGCTGTTAC
CGGTGCTGAACTTCGTGACCATGCCGGGGCTGGTTGGGCCGATCATGGGGCCGATGCTGGGCGGTTGGCTGGTGACCTAC
GCCACCTGGCACTGGATTTTCCTTATCAATATCCCGATTGGCCTGCTCGGCATCTTCTATGCGCGTAAATATATGCCGGA
CTTTACCACCCCCAAGCGCCGCTTCGACTTCATTGGCTTTATGCTGTTTGGCCTGAGCCTGGTGTTGGTTTCTACCGGGC
TGGAACTGTTTGGTGAGCAGGTGCTGGCCAGCTATATCTCATTGGGCATTCTGCTCAGCGGCGTGGTGATGCTGTTCGGC
TATATCGCCCATGCCCGCAGGCATCCTCAACCGCTGATTGGCCTGGACCTGTTCCAGACCCGAACCTTCTCGGTGGGGAT
TGCCGGTAACGTCGCCTCACGGCTGGGCACCGGCTGTGTCCCCTTCCTGATGCCACTGATGTTGCAGGTGGGATTCGGTT
ACTCGGCAATTATTGCCGGTTGCATGATGGCCCCGACCGCGGTCGGATCATTGATGGCGAAATCCACCGTCACTCAGGTG
CTGCGCTGGTTCGGTTATCGTAAAACGCTGGTCGGCATCACCGTCATTATTGGCGTGCTGATTGCTCAGTTCGCGCTGCA
AAGCCCGGGGATGCCGTTATGGTTGATGATCCTGCCGTTGTTCGTGCTGGGGATGGCGATGTCTACGCAATTCACCGCCA
TGAATACCATCAGCCTGGCAGATTTGAATGACGCCAACGCCAGCTCCGGCAATAGCGTGCTGGCAGTAACTCAACAGCTT
TCCATCAGCTTCGGGGTGGCGATCAGCGCGGCGGTACTGCGTTTCTATCAAACCATGTCGCTGGGGACCATGGTCGATCA
CTTCCACTACACCTTTATTACCATGGGGATTGTCACCGTGGCCTCGGCGTTTGTGTTTATGTTACTGAGAAGAAAGGACG
GCCGTAACCTGATCAGCGGCCAGGACCCGAAGAAACCGGTTAAAACCGCGGGTTAA

Upstream 100 bases:

>100_bases
TAACCGCCTGCCAGGTTTTGCTTAGAGAGAAAGACTAACGCCCCCTACTTATATAGAAGAAGATGAAAGGGACGAAGACG
ACAGTTAACCGGGACTCACA

Downstream 100 bases:

>100_bases
CGGTAACCGACCGAAGCACGCTCCACCAGCTCAGGGGTCAACACCAGAACCTGGGCTTCGCGGTCCGGGTTCTGCAGGCG
GTTAATCAGCGTATCAATCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTKSARSMAGLPWIAAMAFFMQALDATILNTALPAIAQSLERSPLAMQSAVISYTLTVAMLIPVSGWLADRFGTRRVFII
AVSLFTLGSLLCALSPTLSLLVTSRVIQGVGGAMMMPVARLALLRAYPRSELLPVLNFVTMPGLVGPIMGPMLGGWLVTY
ATWHWIFLINIPIGLLGIFYARKYMPDFTTPKRRFDFIGFMLFGLSLVLVSTGLELFGEQVLASYISLGILLSGVVMLFG
YIAHARRHPQPLIGLDLFQTRTFSVGIAGNVASRLGTGCVPFLMPLMLQVGFGYSAIIAGCMMAPTAVGSLMAKSTVTQV
LRWFGYRKTLVGITVIIGVLIAQFALQSPGMPLWLMILPLFVLGMAMSTQFTAMNTISLADLNDANASSGNSVLAVTQQL
SISFGVAISAAVLRFYQTMSLGTMVDHFHYTFITMGIVTVASAFVFMLLRRKDGRNLISGQDPKKPVKTAG

Sequences:

>Translated_471_residues
MTKSARSMAGLPWIAAMAFFMQALDATILNTALPAIAQSLERSPLAMQSAVISYTLTVAMLIPVSGWLADRFGTRRVFII
AVSLFTLGSLLCALSPTLSLLVTSRVIQGVGGAMMMPVARLALLRAYPRSELLPVLNFVTMPGLVGPIMGPMLGGWLVTY
ATWHWIFLINIPIGLLGIFYARKYMPDFTTPKRRFDFIGFMLFGLSLVLVSTGLELFGEQVLASYISLGILLSGVVMLFG
YIAHARRHPQPLIGLDLFQTRTFSVGIAGNVASRLGTGCVPFLMPLMLQVGFGYSAIIAGCMMAPTAVGSLMAKSTVTQV
LRWFGYRKTLVGITVIIGVLIAQFALQSPGMPLWLMILPLFVLGMAMSTQFTAMNTISLADLNDANASSGNSVLAVTQQL
SISFGVAISAAVLRFYQTMSLGTMVDHFHYTFITMGIVTVASAFVFMLLRRKDGRNLISGQDPKKPVKTAG
>Mature_470_residues
TKSARSMAGLPWIAAMAFFMQALDATILNTALPAIAQSLERSPLAMQSAVISYTLTVAMLIPVSGWLADRFGTRRVFIIA
VSLFTLGSLLCALSPTLSLLVTSRVIQGVGGAMMMPVARLALLRAYPRSELLPVLNFVTMPGLVGPIMGPMLGGWLVTYA
TWHWIFLINIPIGLLGIFYARKYMPDFTTPKRRFDFIGFMLFGLSLVLVSTGLELFGEQVLASYISLGILLSGVVMLFGY
IAHARRHPQPLIGLDLFQTRTFSVGIAGNVASRLGTGCVPFLMPLMLQVGFGYSAIIAGCMMAPTAVGSLMAKSTVTQVL
RWFGYRKTLVGITVIIGVLIAQFALQSPGMPLWLMILPLFVLGMAMSTQFTAMNTISLADLNDANASSGNSVLAVTQQLS
ISFGVAISAAVLRFYQTMSLGTMVDHFHYTFITMGIVTVASAFVFMLLRRKDGRNLISGQDPKKPVKTAG

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=466, Percent_Identity=77.0386266094421, Blast_Score=730, Evalue=0.0,
Organism=Escherichia coli, GI1788392, Length=451, Percent_Identity=45.4545454545455, Blast_Score=355, Evalue=5e-99,
Organism=Escherichia coli, GI1789042, Length=426, Percent_Identity=28.169014084507, Blast_Score=144, Evalue=9e-36,
Organism=Escherichia coli, GI1788710, Length=431, Percent_Identity=28.538283062645, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI87081983, Length=288, Percent_Identity=31.5972222222222, Blast_Score=125, Evalue=8e-30,
Organism=Saccharomyces cerevisiae, GI6322958, Length=480, Percent_Identity=25.4166666666667, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6321663, Length=149, Percent_Identity=31.5436241610738, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6325455, Length=127, Percent_Identity=31.496062992126, Blast_Score=71, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50870; Mature: 50739

Theoretical pI: Translated: 10.69; Mature: 10.69

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
6.0 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TKSARSMAGLPWIAAMAFFMQALDATILNTALPAIAQSLERSPLAMQSAVISYTLTVAM
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]