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The map label for this gene is trmH [H]

Identifier: 157373101

GI number: 157373101

Start: 5393538

End: 5394233

Strand: Direct

Name: trmH [H]

Synonym: Spro_4870

Alternate gene names: 157373101

Gene position: 5393538-5394233 (Clockwise)

Preceding gene: 157373100

Following gene: 157373102

Centisome position: 98.98

GC content: 58.33

Gene sequence:

>696_bases
ATGAGCCCTGAACGCTATGCGCGGATTTGTGAAATGCTGGCCACCCGGCAGCCGGATTTAACCGTCTGCCTGGAACAGGT
TCATAAACCGCATAACGTCTCCGCCATTATCCGTACCGCCGACGCCGTCGGTGTGCATCAGGTGCATGCCGTGTGGCCAA
CTCCACGCATGCGAACCCTGGTTTCCTCCGCCGCCGGCAGCAACAGTTGGGTGAATGTCAAAACCCATCCCACCATCGTC
GACGCCGTGACCCATTTGAAGGATCAGGGTATGCAGATCCTGGCAACCAACCTCTCCGCCCGCGCAGTAGACTTCCGCGA
GGTGGATTACACCCGCCCGACCTGCGTGCTGCTGGGCCAGGAAAAAACCGGTATTACCGAAGAAGCCCTGCGGCTTGCCG
ATCAGGACATCATCATTCCAATGATCGGTATGGTGCAGTCACTCAACGTGTCGGTCGCCTCAGCATTGATCCTGTACGAA
GCCCAACGCCAGCGGCAAAATGCCGGTCTGTATCGCCGTGAGCACAGCATGCTCGACCAGGAAGAACAACAGCGCCTGCT
GTTTGAAGGTGGTTATCCGGTGCTGGCCAACGTGGCCAAACGTAAAAGACTGCCGCGTCCGCACATTGATGAACAGGGCC
AGGTGGTCGCCGATGCCGAATGGTGGGCAGCCATGCAAGCGACGGTACGCAAATGA

Upstream 100 bases:

>100_bases
GTTTGACCACCCGCGATCGCATCCATCTGGCAAATATTATGCGTAAAATCCGTATCATGCCGGATGTGATTAAAGTTAAC
CGTAACCGAAATTAGCGCTT

Downstream 100 bases:

>100_bases
AAGGCCGCCTGCTGGATGCTGTCCCACTCACCACACTTTCCGGCGTGGGTGCCAGCCAGGCGGGAAAACTGGCCAAAATT
GGCCTGGAAACCATTCAGGA

Product: tRNA guanosine-2'-O-methyltransferase

Products: NA

Alternate protein names: tRNA [Gm18] methyltransferase [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MSPERYARICEMLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGVHQVHAVWPTPRMRTLVSSAAGSNSWVNVKTHPTIV
DAVTHLKDQGMQILATNLSARAVDFREVDYTRPTCVLLGQEKTGITEEALRLADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGLYRREHSMLDQEEQQRLLFEGGYPVLANVAKRKRLPRPHIDEQGQVVADAEWWAAMQATVRK

Sequences:

>Translated_231_residues
MSPERYARICEMLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGVHQVHAVWPTPRMRTLVSSAAGSNSWVNVKTHPTIV
DAVTHLKDQGMQILATNLSARAVDFREVDYTRPTCVLLGQEKTGITEEALRLADQDIIIPMIGMVQSLNVSVASALILYE
AQRQRQNAGLYRREHSMLDQEEQQRLLFEGGYPVLANVAKRKRLPRPHIDEQGQVVADAEWWAAMQATVRK
>Mature_230_residues
SPERYARICEMLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGVHQVHAVWPTPRMRTLVSSAAGSNSWVNVKTHPTIVD
AVTHLKDQGMQILATNLSARAVDFREVDYTRPTCVLLGQEKTGITEEALRLADQDIIIPMIGMVQSLNVSVASALILYEA
QRQRQNAGLYRREHSMLDQEEQQRLLFEGGYPVLANVAKRKRLPRPHIDEQGQVVADAEWWAAMQATVRK

Specific function: Specifically methylates guanosine-18 in various tRNAs [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790083, Length=227, Percent_Identity=82.3788546255507, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1790623, Length=159, Percent_Identity=30.8176100628931, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022724
- InterPro:   IPR001537 [H]

Pfam domain/function: PF12105 SpoU_methylas_C; PF00588 SpoU_methylase [H]

EC number: =2.1.1.34 [H]

Molecular weight: Translated: 25935; Mature: 25804

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPERYARICEMLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGVHQVHAVWPTPRMRTL
CCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHEEEEECCCHHHHHH
VSSAAGSNSWVNVKTHPTIVDAVTHLKDQGMQILATNLSARAVDFREVDYTRPTCVLLGQ
HHHCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEECCCHHEEEHHHCCCCCCEEEEEEC
EKTGITEEALRLADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGLYRREHSMLDQ
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCH
EEQQRLLFEGGYPVLANVAKRKRLPRPHIDEQGQVVADAEWWAAMQATVRK
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHCC
>Mature Secondary Structure 
SPERYARICEMLATRQPDLTVCLEQVHKPHNVSAIIRTADAVGVHQVHAVWPTPRMRTL
CHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHEEEEECCCHHHHHH
VSSAAGSNSWVNVKTHPTIVDAVTHLKDQGMQILATNLSARAVDFREVDYTRPTCVLLGQ
HHHCCCCCCEEEEECCCHHHHHHHHHHHCCCEEEEECCCHHEEEHHHCCCCCCEEEEEEC
EKTGITEEALRLADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGLYRREHSMLDQ
CCCCCHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCH
EEQQRLLFEGGYPVLANVAKRKRLPRPHIDEQGQVVADAEWWAAMQATVRK
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]