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The map label for this gene is rfaG [H]

Identifier: 157373065

GI number: 157373065

Start: 5353521

End: 5354648

Strand: Direct

Name: rfaG [H]

Synonym: Spro_4833

Alternate gene names: 157373065

Gene position: 5353521-5354648 (Clockwise)

Preceding gene: 157373064

Following gene: 157373066

Centisome position: 98.25

GC content: 57.36

Gene sequence:

>1128_bases
ATGAAAGCATTTCGTTTGGCGATCGTTCGCCAAAAATACCGCCCGGACGGTGGCGCTGAGCGTTTCGTTTCCCGCGCCCT
TGAGGCGCTGGAACAACAGGACCTCGACCTGAACGTTATCACCCGCGAATGGCAGGGTGACGCCAATCCAAACTGGCATA
TCCACCTGTGTAATCCGATAAAATTCGGCCGCATCAGCCGTGAACGTGGCTTTGCCGAAGCGGCCAGAGCACTGTGGCAG
AAAGAAAACTTCGATCTGGTGCAAAGCCATGAACGCATCCCAGGCTGCGATATTTACCGCGCCGGTGATGGCGTGCATCG
CCGCTGGTTACTGCAGCGGGCTCGTTTGTTGCCGGAGTGGCGTCGTAAATGGCTGTTCTCCAATCGCTATCACCGTTACG
TGATGTGCGCAGAACGGGCAATGTACGCAGCGCCGGAGCTGAAGGCCGTTATCTGCAATGCCGAAATGATTAAGCAGGAA
ATCATCGATGACTTCGGAGTCCCCGCCGATAAAATCACGGTGATCTACAATGCCATTGATAATCAAAAATTTCCGCCCGC
CGATGCCGAACTACGCCAGCAGTTGCGTCTGCAGTATCAGATCCCGCAGCAGGCTCACTGCCTGGTTTTTGTCGGTTCCG
GTTTTGAACGCAAAGGGCTGGCCGCGGCCATCCGTGCCGTCGCAGCGACCGACAGCTATCTGCTGGTGGTAGGTAAGGAT
AAGGCGGAAAAACGTTATCAGGCGCTGGCGCAATCACTGGGCTGCAGCGATCGGGTGCGATTTATGGGGGTGCAGAAACA
AACGCTGCCGTTCTATCAGGCCGCCGATGCGCTGTTGCTGCCCACGTTGTACGATCCGTTCCCCAACGTGATTCTGGAAG
CCATGTCCTGCGGTTTGCCGGTCATTACCAGCACCACCTGCGGCGGCTCGGAGTTTATCACTCAGGGGCAAAATGGCTTT
GTTACCGATGCCCTTGATGTCCCCGCGCTGGTCGCGGCGATCGTAGCGCTACCACGCCAGGCCCTGGGCTCCGCCATGGG
TGAGGCAGCAAGAGCGCGTATCATGTCGGCTACCCCGGCGCGCCTGTCGGAACAGCTGATCTCCCTTTATAACCGGCTGC
TGGATTAA

Upstream 100 bases:

>100_bases
ACTTACCCGATCCGGACGCCATCGATACCGGCACCGATGAACGTTATCTGGACCTTATTCCTACAGACGCGGTGATTGCA
GCCGCGCGGAGCCAGCTTGC

Downstream 100 bases:

>100_bases
CTATGCGTATTCTGATGATTATCGACGGCCTGCCTGGCGGAGGCGCCGAAAAAGTGGTGCTGACCCTGTGCCAGGGAATG
CAGCAAATGGGTCATGACGT

Product: group 1 glycosyl transferase

Products: NA

Alternate protein names: Glucosyltransferase I [H]

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MKAFRLAIVRQKYRPDGGAERFVSRALEALEQQDLDLNVITREWQGDANPNWHIHLCNPIKFGRISRERGFAEAARALWQ
KENFDLVQSHERIPGCDIYRAGDGVHRRWLLQRARLLPEWRRKWLFSNRYHRYVMCAERAMYAAPELKAVICNAEMIKQE
IIDDFGVPADKITVIYNAIDNQKFPPADAELRQQLRLQYQIPQQAHCLVFVGSGFERKGLAAAIRAVAATDSYLLVVGKD
KAEKRYQALAQSLGCSDRVRFMGVQKQTLPFYQAADALLLPTLYDPFPNVILEAMSCGLPVITSTTCGGSEFITQGQNGF
VTDALDVPALVAAIVALPRQALGSAMGEAARARIMSATPARLSEQLISLYNRLLD

Sequences:

>Translated_375_residues
MKAFRLAIVRQKYRPDGGAERFVSRALEALEQQDLDLNVITREWQGDANPNWHIHLCNPIKFGRISRERGFAEAARALWQ
KENFDLVQSHERIPGCDIYRAGDGVHRRWLLQRARLLPEWRRKWLFSNRYHRYVMCAERAMYAAPELKAVICNAEMIKQE
IIDDFGVPADKITVIYNAIDNQKFPPADAELRQQLRLQYQIPQQAHCLVFVGSGFERKGLAAAIRAVAATDSYLLVVGKD
KAEKRYQALAQSLGCSDRVRFMGVQKQTLPFYQAADALLLPTLYDPFPNVILEAMSCGLPVITSTTCGGSEFITQGQNGF
VTDALDVPALVAAIVALPRQALGSAMGEAARARIMSATPARLSEQLISLYNRLLD
>Mature_375_residues
MKAFRLAIVRQKYRPDGGAERFVSRALEALEQQDLDLNVITREWQGDANPNWHIHLCNPIKFGRISRERGFAEAARALWQ
KENFDLVQSHERIPGCDIYRAGDGVHRRWLLQRARLLPEWRRKWLFSNRYHRYVMCAERAMYAAPELKAVICNAEMIKQE
IIDDFGVPADKITVIYNAIDNQKFPPADAELRQQLRLQYQIPQQAHCLVFVGSGFERKGLAAAIRAVAATDSYLLVVGKD
KAEKRYQALAQSLGCSDRVRFMGVQKQTLPFYQAADALLLPTLYDPFPNVILEAMSCGLPVITSTTCGGSEFITQGQNGF
VTDALDVPALVAAIVALPRQALGSAMGEAARARIMSATPARLSEQLISLYNRLLD

Specific function: Involved in the addition of the first glucose residue to the lipopolysaccharide core [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790061, Length=328, Percent_Identity=25.9146341463415, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296 [H]

Pfam domain/function: PF00534 Glycos_transf_1 [H]

EC number: NA

Molecular weight: Translated: 42193; Mature: 42193

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFRLAIVRQKYRPDGGAERFVSRALEALEQQDLDLNVITREWQGDANPNWHIHLCNPI
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCC
KFGRISRERGFAEAARALWQKENFDLVQSHERIPGCDIYRAGDGVHRRWLLQRARLLPEW
CCCCCHHHCCHHHHHHHHHCCCCCHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHCHHH
RRKWLFSNRYHRYVMCAERAMYAAPELKAVICNAEMIKQEIIDDFGVPADKITVIYNAID
HHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEHHC
NQKFPPADAELRQQLRLQYQIPQQAHCLVFVGSGFERKGLAAAIRAVAATDSYLLVVGKD
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECC
KAEKRYQALAQSLGCSDRVRFMGVQKQTLPFYQAADALLLPTLYDPFPNVILEAMSCGLP
HHHHHHHHHHHHCCCCCCHHHCCCCHHHCCHHHHCCHHHCCHHHCHHHHHHHHHHHCCCC
VITSTTCGGSEFITQGQNGFVTDALDVPALVAAIVALPRQALGSAMGEAARARIMSATPA
EEEECCCCCHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RLSEQLISLYNRLLD
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MKAFRLAIVRQKYRPDGGAERFVSRALEALEQQDLDLNVITREWQGDANPNWHIHLCNPI
CCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEEEECCC
KFGRISRERGFAEAARALWQKENFDLVQSHERIPGCDIYRAGDGVHRRWLLQRARLLPEW
CCCCCHHHCCHHHHHHHHHCCCCCHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHCHHH
RRKWLFSNRYHRYVMCAERAMYAAPELKAVICNAEMIKQEIIDDFGVPADKITVIYNAID
HHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHEEEEEEHHC
NQKFPPADAELRQQLRLQYQIPQQAHCLVFVGSGFERKGLAAAIRAVAATDSYLLVVGKD
CCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCEEEEEECC
KAEKRYQALAQSLGCSDRVRFMGVQKQTLPFYQAADALLLPTLYDPFPNVILEAMSCGLP
HHHHHHHHHHHHCCCCCCHHHCCCCHHHCCHHHHCCHHHCCHHHCHHHHHHHHHHHCCCC
VITSTTCGGSEFITQGQNGFVTDALDVPALVAAIVALPRQALGSAMGEAARARIMSATPA
EEEECCCCCHHHHHCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
RLSEQLISLYNRLLD
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Hexosyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732225; 8041620; 9278503; 1447141; 7988890 [H]