| Definition | Serratia proteamaculans 568 chromosome, complete genome. |
|---|---|
| Accession | NC_009832 |
| Length | 5,448,853 |
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The map label for this gene is dkgA [H]
Identifier: 157372449
GI number: 157372449
Start: 4673583
End: 4674407
Strand: Direct
Name: dkgA [H]
Synonym: Spro_4216
Alternate gene names: 157372449
Gene position: 4673583-4674407 (Clockwise)
Preceding gene: 157372448
Following gene: 157372453
Centisome position: 85.77
GC content: 56.0
Gene sequence:
>825_bases ATGACAACGCAACAACCCATCATCAAACTCCACGATGGTAATCTGATGCCGCAGTTGGGTCTCGGCGTTTGGCAGGCGAG TATTCCAGACACCACCCAGGCGGTGCTCAAGGCGCTGGAGGTGGGATATCGTTCGATCGATACCGCTGCGATTTACAAGA ACGAGGAAGGGGTCGGTGCTGCACTGCAATCCGCCTCGGTGCCGCGCGATGAACTGTTTATTACCACCAAACTGTGGAAC GACGATCAGGGCGACCCACAGGCCGCCCTGGAAACCAGCCTGAAAAAACTCAAGCTGGACTACGTCGATCTGTATCTGAT CCACTGGCCAAAACCGCAGCAGGATCAATACGTAGACGCCTGGCGCGGGCTGATCAAACTGCGCGAACAGGGGCTGGTGA AGAGTATCGGGGTCTGCAATTTCCATACCCCGCATCTGCAGCGCCTGCTGGACGAAACCAATATCGCGCCGGTGATTAAC CAGATTGAACTGCATCCGTTGCTGCAACAACGTCAGTTGCGCGCCTGGAACGCAACGCACCACATCGCTACCGAATCATG GAGCCCACTGGCGCAGGGCGGCGAAGGGGTGTTCGACCAGCCGGTCATCAAACAGCTGGCGGAAAAATACGAGAAGACCC CGGCGCAAATCGTGGTGCGCTGGCACCTGGACAGCGGTCTTATCGTGATCCCGAAATCGGTCACACCATCACGCATTCGT GAAAACTTTGAGGTGTTTGATTTCAAACTGGATAAAGACGAACTGAGCGATATCGCCCAACTGGATATCGGCAACCGTCT GGGGCCGGATCCCGACTCACTGTAA
Upstream 100 bases:
>100_bases CTTAGCAAACTCAGCACCACCCCAGTTTGCCAACTAGAATTTATTGATAGCCGTTCCTGGTGCATGCAGCACCGGGCAAT CCGTTAACAAGGAGATACGC
Downstream 100 bases:
>100_bases TGTCCCAGCGCCCGGCTTCCGCCGGGCGCTGCATGGCCCCGTAAAGGGGGGTTATGATTGTCCAGGCCGTGCTGGATGGG TAGCAAGTCGGCAGGCAATA
Product: 2,5-diketo-D-gluconate reductase A
Products: NA
Alternate protein names: 2,5-DKG reductase A; 2,5-DKGR A; 25DKGR-A; AKR5C [H]
Number of amino acids: Translated: 274; Mature: 273
Protein sequence:
>274_residues MTTQQPIIKLHDGNLMPQLGLGVWQASIPDTTQAVLKALEVGYRSIDTAAIYKNEEGVGAALQSASVPRDELFITTKLWN DDQGDPQAALETSLKKLKLDYVDLYLIHWPKPQQDQYVDAWRGLIKLREQGLVKSIGVCNFHTPHLQRLLDETNIAPVIN QIELHPLLQQRQLRAWNATHHIATESWSPLAQGGEGVFDQPVIKQLAEKYEKTPAQIVVRWHLDSGLIVIPKSVTPSRIR ENFEVFDFKLDKDELSDIAQLDIGNRLGPDPDSL
Sequences:
>Translated_274_residues MTTQQPIIKLHDGNLMPQLGLGVWQASIPDTTQAVLKALEVGYRSIDTAAIYKNEEGVGAALQSASVPRDELFITTKLWN DDQGDPQAALETSLKKLKLDYVDLYLIHWPKPQQDQYVDAWRGLIKLREQGLVKSIGVCNFHTPHLQRLLDETNIAPVIN QIELHPLLQQRQLRAWNATHHIATESWSPLAQGGEGVFDQPVIKQLAEKYEKTPAQIVVRWHLDSGLIVIPKSVTPSRIR ENFEVFDFKLDKDELSDIAQLDIGNRLGPDPDSL >Mature_273_residues TTQQPIIKLHDGNLMPQLGLGVWQASIPDTTQAVLKALEVGYRSIDTAAIYKNEEGVGAALQSASVPRDELFITTKLWND DQGDPQAALETSLKKLKLDYVDLYLIHWPKPQQDQYVDAWRGLIKLREQGLVKSIGVCNFHTPHLQRLLDETNIAPVINQ IELHPLLQQRQLRAWNATHHIATESWSPLAQGGEGVFDQPVIKQLAEKYEKTPAQIVVRWHLDSGLIVIPKSVTPSRIRE NFEVFDFKLDKDELSDIAQLDIGNRLGPDPDSL
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI223468663, Length=290, Percent_Identity=37.5862068965517, Blast_Score=197, Evalue=8e-51, Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=36.6782006920415, Blast_Score=196, Evalue=2e-50, Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=36.6782006920415, Blast_Score=196, Evalue=2e-50, Organism=Homo sapiens, GI93277124, Length=303, Percent_Identity=36.6336633663366, Blast_Score=195, Evalue=3e-50, Organism=Homo sapiens, GI310109922, Length=286, Percent_Identity=37.4125874125874, Blast_Score=184, Evalue=9e-47, Organism=Homo sapiens, GI5453543, Length=304, Percent_Identity=36.1842105263158, Blast_Score=183, Evalue=1e-46, Organism=Homo sapiens, GI310109920, Length=304, Percent_Identity=36.1842105263158, Blast_Score=183, Evalue=2e-46, Organism=Homo sapiens, GI45446745, Length=304, Percent_Identity=36.1842105263158, Blast_Score=183, Evalue=2e-46, Organism=Homo sapiens, GI4503285, Length=304, Percent_Identity=36.1842105263158, Blast_Score=183, Evalue=2e-46, Organism=Homo sapiens, GI24497583, Length=300, Percent_Identity=34.6666666666667, Blast_Score=172, Evalue=3e-43, Organism=Homo sapiens, GI291291012, Length=264, Percent_Identity=36.7424242424242, Blast_Score=172, Evalue=4e-43, Organism=Homo sapiens, GI4502049, Length=283, Percent_Identity=38.1625441696113, Blast_Score=170, Evalue=1e-42, Organism=Homo sapiens, GI24497585, Length=297, Percent_Identity=37.3737373737374, Blast_Score=167, Evalue=7e-42, Organism=Homo sapiens, GI5174695, Length=299, Percent_Identity=35.7859531772575, Blast_Score=166, Evalue=3e-41, Organism=Homo sapiens, GI300116273, Length=277, Percent_Identity=35.7400722021661, Blast_Score=148, Evalue=5e-36, Organism=Homo sapiens, GI300116271, Length=282, Percent_Identity=32.9787234042553, Blast_Score=132, Evalue=5e-31, Organism=Homo sapiens, GI310109926, Length=119, Percent_Identity=45.3781512605042, Blast_Score=100, Evalue=2e-21, Organism=Homo sapiens, GI207028673, Length=119, Percent_Identity=45.3781512605042, Blast_Score=100, Evalue=2e-21, Organism=Homo sapiens, GI310109924, Length=152, Percent_Identity=34.8684210526316, Blast_Score=96, Evalue=4e-20, Organism=Escherichia coli, GI87082198, Length=268, Percent_Identity=74.2537313432836, Blast_Score=419, Evalue=1e-118, Organism=Escherichia coli, GI1786400, Length=258, Percent_Identity=39.1472868217054, Blast_Score=182, Evalue=1e-47, Organism=Escherichia coli, GI1788081, Length=274, Percent_Identity=29.1970802919708, Blast_Score=102, Evalue=4e-23, Organism=Caenorhabditis elegans, GI17550248, Length=299, Percent_Identity=37.4581939799331, Blast_Score=188, Evalue=3e-48, Organism=Caenorhabditis elegans, GI17537077, Length=293, Percent_Identity=36.1774744027304, Blast_Score=186, Evalue=1e-47, Organism=Caenorhabditis elegans, GI17537075, Length=298, Percent_Identity=36.241610738255, Blast_Score=184, Evalue=6e-47, Organism=Caenorhabditis elegans, GI17566692, Length=289, Percent_Identity=35.2941176470588, Blast_Score=179, Evalue=1e-45, Organism=Caenorhabditis elegans, GI17564128, Length=298, Percent_Identity=35.9060402684564, Blast_Score=175, Evalue=2e-44, Organism=Caenorhabditis elegans, GI71998625, Length=269, Percent_Identity=36.8029739776952, Blast_Score=172, Evalue=2e-43, Organism=Caenorhabditis elegans, GI17538386, Length=274, Percent_Identity=31.3868613138686, Blast_Score=158, Evalue=3e-39, Organism=Caenorhabditis elegans, GI17561298, Length=272, Percent_Identity=32.3529411764706, Blast_Score=157, Evalue=8e-39, Organism=Caenorhabditis elegans, GI17562292, Length=277, Percent_Identity=34.2960288808664, Blast_Score=154, Evalue=5e-38, Organism=Caenorhabditis elegans, GI17552492, Length=275, Percent_Identity=29.8181818181818, Blast_Score=146, Evalue=1e-35, Organism=Caenorhabditis elegans, GI17561300, Length=271, Percent_Identity=31.7343173431734, Blast_Score=145, Evalue=3e-35, Organism=Caenorhabditis elegans, GI17537079, Length=298, Percent_Identity=26.8456375838926, Blast_Score=131, Evalue=3e-31, Organism=Saccharomyces cerevisiae, GI6320576, Length=282, Percent_Identity=38.2978723404255, Blast_Score=206, Evalue=4e-54, Organism=Saccharomyces cerevisiae, GI6324694, Length=283, Percent_Identity=38.1625441696113, Blast_Score=205, Evalue=6e-54, Organism=Saccharomyces cerevisiae, GI6319625, Length=300, Percent_Identity=35.3333333333333, Blast_Score=162, Evalue=5e-41, Organism=Saccharomyces cerevisiae, GI6322556, Length=260, Percent_Identity=31.1538461538462, Blast_Score=149, Evalue=5e-37, Organism=Saccharomyces cerevisiae, GI6321896, Length=313, Percent_Identity=32.2683706070287, Blast_Score=146, Evalue=3e-36, Organism=Saccharomyces cerevisiae, GI6320079, Length=290, Percent_Identity=32.4137931034483, Blast_Score=132, Evalue=5e-32, Organism=Drosophila melanogaster, GI20129731, Length=296, Percent_Identity=38.5135135135135, Blast_Score=201, Evalue=4e-52, Organism=Drosophila melanogaster, GI24662789, Length=297, Percent_Identity=38.047138047138, Blast_Score=196, Evalue=1e-50, Organism=Drosophila melanogaster, GI21356425, Length=301, Percent_Identity=36.5448504983389, Blast_Score=194, Evalue=4e-50, Organism=Drosophila melanogaster, GI24657054, Length=294, Percent_Identity=39.4557823129252, Blast_Score=193, Evalue=1e-49, Organism=Drosophila melanogaster, GI24644950, Length=301, Percent_Identity=36.2126245847176, Blast_Score=190, Evalue=1e-48, Organism=Drosophila melanogaster, GI24663317, Length=295, Percent_Identity=35.9322033898305, Blast_Score=183, Evalue=1e-46, Organism=Drosophila melanogaster, GI221378297, Length=321, Percent_Identity=34.2679127725857, Blast_Score=182, Evalue=2e-46, Organism=Drosophila melanogaster, GI45553081, Length=293, Percent_Identity=35.8361774744027, Blast_Score=173, Evalue=1e-43, Organism=Drosophila melanogaster, GI24662781, Length=296, Percent_Identity=40.5405405405405, Blast_Score=173, Evalue=1e-43, Organism=Drosophila melanogaster, GI24662785, Length=298, Percent_Identity=40.2684563758389, Blast_Score=171, Evalue=4e-43, Organism=Drosophila melanogaster, GI281366140, Length=258, Percent_Identity=34.8837209302326, Blast_Score=145, Evalue=2e-35,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 30873; Mature: 30741
Theoretical pI: Translated: 5.35; Mature: 5.35
Prosite motif: PS00798 ALDOKETO_REDUCTASE_1 ; PS00062 ALDOKETO_REDUCTASE_2 ; PS00063 ALDOKETO_REDUCTASE_3
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 0.7 %Met (Translated Protein) 1.1 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 0.4 %Met (Mature Protein) 0.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MTTQQPIIKLHDGNLMPQLGLGVWQASIPDTTQAVLKALEVGYRSIDTAAIYKNEEGVGA CCCCCCEEEEECCCCCCHHCCCEEECCCCHHHHHHHHHHHHHHHHCCHHEEEECCCCCHH ALQSASVPRDELFITTKLWNDDQGDPQAALETSLKKLKLDYVDLYLIHWPKPQQDQYVDA HHHHCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHHH WRGLIKLREQGLVKSIGVCNFHTPHLQRLLDETNIAPVINQIELHPLLQQRQLRAWNATH HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHH HIATESWSPLAQGGEGVFDQPVIKQLAEKYEKTPAQIVVRWHLDSGLIVIPKSVTPSRIR HCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCEEEEECCCCHHHHH ENFEVFDFKLDKDELSDIAQLDIGNRLGPDPDSL CCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCC >Mature Secondary Structure TTQQPIIKLHDGNLMPQLGLGVWQASIPDTTQAVLKALEVGYRSIDTAAIYKNEEGVGA CCCCCEEEEECCCCCCHHCCCEEECCCCHHHHHHHHHHHHHHHHCCHHEEEECCCCCHH ALQSASVPRDELFITTKLWNDDQGDPQAALETSLKKLKLDYVDLYLIHWPKPQQDQYVDA HHHHCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHHH WRGLIKLREQGLVKSIGVCNFHTPHLQRLLDETNIAPVINQIELHPLLQQRQLRAWNATH HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCHH HIATESWSPLAQGGEGVFDQPVIKQLAEKYEKTPAQIVVRWHLDSGLIVIPKSVTPSRIR HCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCEEEEECCCCHHHHH ENFEVFDFKLDKDELSDIAQLDIGNRLGPDPDSL CCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11586360; 12142430 [H]