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The map label for this gene is aceF [H]

Identifier: 157372244

GI number: 157372244

Start: 4446729

End: 4448621

Strand: Reverse

Name: aceF [H]

Synonym: Spro_4010

Alternate gene names: 157372244

Gene position: 4448621-4446729 (Counterclockwise)

Preceding gene: 157372245

Following gene: 157372243

Centisome position: 81.64

GC content: 56.15

Gene sequence:

>1893_bases
ATGTCTATCGAAATTAAAGTACCGGACATCGGTGCAGACGAAGTGGAAATCACCGAAATTCTGGTGAAAGTGGGCGATAA
AGTTGAAGCTGAACAATCGCTGATCACCGTTGAAGGTGATAAAGCTTCCATGGAAGTCCCATCCCCGCAGGCGGGTGTGG
TTAAAGAAATCAAGGTTGCCGTCGGTGATAAAACCGAAACCGGCAAACTGCTGATGATCTTTGAAGCAGAAGGGGCGGCT
CAGGCCGCACCAGCTGCCAAGGCTGAAGAGAAACCGGCCGCAGCACCTGCTGCTGCACCGGCTGCCGCTGCTGCCAAAGA
CGTTGCGGTACCGGACATCGGTGCTGACGAAGTTGAAGTGACCGAGATCCTGGTGAAAGTGGGCGACAAGGTGGAAGCCG
AGCAGTCCCTGATCACCGTTGAAGGCGACAAGGCTTCAATGGAAGTCCCGGCACCGTTCGCAGGTACCGTGAAAGAGATC
AAAATCGCCACCGGCGACAAAGTCACCACCGGTTCTATGATCATGGTGTTCGAAGTGGCTGGCGCCGCTTCTGCTGCCGC
TCCGGCTGCGGCACAAGCACCGGCTGCTCCTGCAGCACCGGCGGCTTCTGCGGCTTCTGCGGCCAAAGACGTTGCAGTAC
CGGACATCGGCGGCGACGAAGTAGAAGTCACTGAAGTGATGGTTAAAGTGGGCGACAAAGTTGCCGCTGAGCAGTCACTG
ATCACCGTTGAAGGTGACAAGGCTTCTATGGAAGTGCCGGCACCGTTCGCAGGTACCGTGAAAGAAATTAAAATCAGCGC
CGGCGATAAAGTAAAAACCGGTTCCCTGATCATGGTGTTCGAAGTTGAAGGCGCTGCGCCTGCTGCCGCTCCAGCCCAGA
AGGCAGAAGCTGCACCGGCTCCGGCTGCAAGTTCTGCACCGGCCCAACAGGCTGCACCTGCTGCCAAAGGCGAGTTCACT
GAGAACGACGCTTATGTGCACGCTACCCCGGTCATCCGCCGTCTGGCGCGTGAGTTCGGCGTAAACCTGGCGAAAGTGAA
AGGCACCGGTCGTAAAGGCCGCATCCTGCGCGAAGACGTTCAGACTTACGTGAAAGACGCGGTCAAACGTGCAGAAGCGG
CTCCGGCTGCTGCTGCCGGTGGCGGTCTGCCTGGCATGCTGCCTTGGCCGAAAGTGGACTTCAGCAAGTTCGGTGAAATC
GAAGAAGTGGAACTGGGTCGTATCCAGAAAATCTCTGGTGCCAACCTGAGCCGTAACTGGGTGATGATCCCGCACGTGAC
TCACTTCGACAAAACCGATATCACCGATCTGGAAGCTTTCCGCAAGCAACAGAACGAAGAAGCTGCCAAGCGTAAGCTGG
ACGTGAAGTTCACCCCGGTGGTGTTCATCATGAAAGCCGTTGCTGCTGCTCTGGAGCAGATGCCGCGCTTCAACAGCTCT
CTGTCCGAAGATGGCCAGAAGCTGACGTTGAAGAAATACATCAACGTGGGTGTGGCGGTTGATACGCCAAACGGTCTGGT
GGTTCCGGTGTTCAAGGATGTGAACAAGAAGAGCATCACTGAACTGTCCCGCGAACTGATGGCTATCTCCAAAAAAGCTC
GTGACGGCAAGCTGACCGCAGGCGAAATGCAGGGCGGGTGTTTCACCATCTCTAGCCTGGGCGGTATCGGGACGACTCAC
TTCGCACCGATTGTGAATGCACCGGAAGTGGCCATTCTGGGCGTCTCCAAGTCTGCTATGGAGCCGGTCTGGAACGGTAA
AGAGTTCGTGCCGCGCCTGATGATGCCAATGTCGCTCTCCTTCGACCACCGTGTTATTGACGGTGCAGATGGTGCGCGCT
TTATTACCATCATCAACAACATGCTGGCTGACATCCGCCGTATGGTGATGTAA

Upstream 100 bases:

>100_bases
CGCGGTGAAATCGAAGCTTCTGTGGTAGCTGATGCGATTAAGAAATTCGACATCAACCCAGAAAAAGTTAACCCGCGTCT
GGCATAAGAGGTACAGAAGA

Downstream 100 bases:

>100_bases
TGAAAAAAGGGCGCAGCATGCTGCGCCCTTACCACTTCAGTAGCAGTTTTGTCGGTTTTTGCACGACTCGACAAAATTGT
TGAGACACGGGTCACTCGAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 630; Mature: 629

Protein sequence:

>630_residues
MSIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVAVGDKTETGKLLMIFEAEGAA
QAAPAAKAEEKPAAAPAAAPAAAAAKDVAVPDIGADEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
KIATGDKVTTGSMIMVFEVAGAASAAAPAAAQAPAAPAAPAASAASAAKDVAVPDIGGDEVEVTEVMVKVGDKVAAEQSL
ITVEGDKASMEVPAPFAGTVKEIKISAGDKVKTGSLIMVFEVEGAAPAAAPAQKAEAAPAPAASSAPAQQAAPAAKGEFT
ENDAYVHATPVIRRLAREFGVNLAKVKGTGRKGRILREDVQTYVKDAVKRAEAAPAAAAGGGLPGMLPWPKVDFSKFGEI
EEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITDLEAFRKQQNEEAAKRKLDVKFTPVVFIMKAVAAALEQMPRFNSS
LSEDGQKLTLKKYINVGVAVDTPNGLVVPVFKDVNKKSITELSRELMAISKKARDGKLTAGEMQGGCFTISSLGGIGTTH
FAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMMPMSLSFDHRVIDGADGARFITIINNMLADIRRMVM

Sequences:

>Translated_630_residues
MSIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVAVGDKTETGKLLMIFEAEGAA
QAAPAAKAEEKPAAAPAAAPAAAAAKDVAVPDIGADEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
KIATGDKVTTGSMIMVFEVAGAASAAAPAAAQAPAAPAAPAASAASAAKDVAVPDIGGDEVEVTEVMVKVGDKVAAEQSL
ITVEGDKASMEVPAPFAGTVKEIKISAGDKVKTGSLIMVFEVEGAAPAAAPAQKAEAAPAPAASSAPAQQAAPAAKGEFT
ENDAYVHATPVIRRLAREFGVNLAKVKGTGRKGRILREDVQTYVKDAVKRAEAAPAAAAGGGLPGMLPWPKVDFSKFGEI
EEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITDLEAFRKQQNEEAAKRKLDVKFTPVVFIMKAVAAALEQMPRFNSS
LSEDGQKLTLKKYINVGVAVDTPNGLVVPVFKDVNKKSITELSRELMAISKKARDGKLTAGEMQGGCFTISSLGGIGTTH
FAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMMPMSLSFDHRVIDGADGARFITIINNMLADIRRMVM
>Mature_629_residues
SIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVAVGDKTETGKLLMIFEAEGAAQ
AAPAAKAEEKPAAAPAAAPAAAAAKDVAVPDIGADEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEIK
IATGDKVTTGSMIMVFEVAGAASAAAPAAAQAPAAPAAPAASAASAAKDVAVPDIGGDEVEVTEVMVKVGDKVAAEQSLI
TVEGDKASMEVPAPFAGTVKEIKISAGDKVKTGSLIMVFEVEGAAPAAAPAQKAEAAPAPAASSAPAQQAAPAAKGEFTE
NDAYVHATPVIRRLAREFGVNLAKVKGTGRKGRILREDVQTYVKDAVKRAEAAPAAAAGGGLPGMLPWPKVDFSKFGEIE
EVELGRIQKISGANLSRNWVMIPHVTHFDKTDITDLEAFRKQQNEEAAKRKLDVKFTPVVFIMKAVAAALEQMPRFNSSL
SEDGQKLTLKKYINVGVAVDTPNGLVVPVFKDVNKKSITELSRELMAISKKARDGKLTAGEMQGGCFTISSLGGIGTTHF
APIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMMPMSLSFDHRVIDGADGARFITIINNMLADIRRMVM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=500, Percent_Identity=29, Blast_Score=174, Evalue=3e-43,
Organism=Homo sapiens, GI31711992, Length=416, Percent_Identity=30.7692307692308, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI203098816, Length=483, Percent_Identity=27.1221532091097, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI19923748, Length=205, Percent_Identity=33.1707317073171, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI203098753, Length=432, Percent_Identity=28.0092592592593, Blast_Score=122, Evalue=2e-27,
Organism=Homo sapiens, GI260898739, Length=157, Percent_Identity=36.9426751592357, Blast_Score=95, Evalue=3e-19,
Organism=Escherichia coli, GI1786305, Length=633, Percent_Identity=85.3080568720379, Blast_Score=978, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=425, Percent_Identity=31.2941176470588, Blast_Score=171, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=400, Percent_Identity=29.5, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17560088, Length=431, Percent_Identity=28.538283062645, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25146366, Length=207, Percent_Identity=36.231884057971, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI17538894, Length=308, Percent_Identity=27.5974025974026, Blast_Score=97, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=412, Percent_Identity=28.3980582524272, Blast_Score=164, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324258, Length=431, Percent_Identity=27.8422273781903, Blast_Score=116, Evalue=9e-27,
Organism=Drosophila melanogaster, GI18859875, Length=424, Percent_Identity=31.3679245283019, Blast_Score=175, Evalue=9e-44,
Organism=Drosophila melanogaster, GI24645909, Length=215, Percent_Identity=33.953488372093, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=30.6382978723404, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=30.6382978723404, Blast_Score=113, Evalue=4e-25,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 65709; Mature: 65578

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVA
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHEEEE
VGDKTETGKLLMIFEAEGAAQAAPAAKAEEKPAAAPAAAPAAAAAKDVAVPDIGADEVEV
ECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHH
TEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEIKIATGDKVTTGSMIMVFEVA
HHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCCEEEEEECCCEEECCCEEEEEEEC
GAASAAAPAAAQAPAAPAAPAASAASAAKDVAVPDIGGDEVEVTEVMVKVGDKVAAEQSL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHCCCEE
ITVEGDKASMEVPAPFAGTVKEIKISAGDKVKTGSLIMVFEVEGAAPAAAPAQKAEAAPA
EEEECCCCCEECCCCCCCCCEEEEECCCCCEECCCEEEEEEECCCCCCCCCHHHHCCCCC
PAASSAPAQQAAPAAKGEFTENDAYVHATPVIRRLAREFGVNLAKVKGTGRKGRILREDV
CCCCCCCHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHH
QTYVKDAVKRAEAAPAAAAGGGLPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNW
HHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHCCCCCEECCCCEEEECCCCCCCCE
VMIPHVTHFDKTDITDLEAFRKQQNEEAAKRKLDVKFTPVVFIMKAVAAALEQMPRFNSS
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCH
LSEDGQKLTLKKYINVGVAVDTPNGLVVPVFKDVNKKSITELSRELMAISKKARDGKLTA
HHCCCCEEEEEHHCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEE
GEMQGGCFTISSLGGIGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMMPMSLS
CCCCCCEEEECCCCCCCCHHHHCCCCCCCEEEEECCHHHCCCCCCCHHHHHHHHCCCCCC
FDHRVIDGADGARFITIINNMLADIRRMVM
CCCEEECCCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASMEVPSPQAGVVKEIKVA
EEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHEEEE
VGDKTETGKLLMIFEAEGAAQAAPAAKAEEKPAAAPAAAPAAAAAKDVAVPDIGADEVEV
ECCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHH
TEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEIKIATGDKVTTGSMIMVFEVA
HHHHHHHCCCCCCCCCEEEEECCCCCEECCCCCCCCCEEEEEECCCEEECCCEEEEEEEC
GAASAAAPAAAQAPAAPAAPAASAASAAKDVAVPDIGGDEVEVTEVMVKVGDKVAAEQSL
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHHCCCEE
ITVEGDKASMEVPAPFAGTVKEIKISAGDKVKTGSLIMVFEVEGAAPAAAPAQKAEAAPA
EEEECCCCCEECCCCCCCCCEEEEECCCCCEECCCEEEEEEECCCCCCCCCHHHHCCCCC
PAASSAPAQQAAPAAKGEFTENDAYVHATPVIRRLAREFGVNLAKVKGTGRKGRILREDV
CCCCCCCHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHH
QTYVKDAVKRAEAAPAAAAGGGLPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNW
HHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCCHHHCCCCCEECCCCEEEECCCCCCCCE
VMIPHVTHFDKTDITDLEAFRKQQNEEAAKRKLDVKFTPVVFIMKAVAAALEQMPRFNSS
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCCCCCH
LSEDGQKLTLKKYINVGVAVDTPNGLVVPVFKDVNKKSITELSRELMAISKKARDGKLTA
HHCCCCEEEEEHHCCCEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCEE
GEMQGGCFTISSLGGIGTTHFAPIVNAPEVAILGVSKSAMEPVWNGKEFVPRLMMPMSLS
CCCCCCEEEECCCCCCCCHHHHCCCCCCCEEEEECCHHHCCCCCCCHHHHHHHHCCCCCC
FDHRVIDGADGARFITIINNMLADIRRMVM
CCCEEECCCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]