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The map label for this gene is lpdA [H]

Identifier: 157372243

GI number: 157372243

Start: 4445071

End: 4446495

Strand: Reverse

Name: lpdA [H]

Synonym: Spro_4009

Alternate gene names: 157372243

Gene position: 4446495-4445071 (Counterclockwise)

Preceding gene: 157372244

Following gene: 157372242

Centisome position: 81.6

GC content: 54.88

Gene sequence:

>1425_bases
ATGAGTACTGAAATTAAAACTCAGGTCGTGGTACTTGGGGCGGGCCCGGCAGGTTACTCTGCTGCCTTTCGTTGCGCTGA
CTTAGGTCTTGAAACCGTTCTGGTTGAACGTTATTCCACTCTGGGCGGGGTTTGCCTGAACGTGGGATGTATCCCTTCCA
AAGCCCTGTTGCACGTTGCCAAAGTGATCGAAGAAGCCAAAGCCCTGGCCGAACACGGCATCGTTTTCGGCGAGCCGAAA
ACTGACATCGACAAAGTGCGCGTCTGGAAAGAAAAAGTCATCAATCAGCTGACCGGCGGTCTGGCTGGTATGGCTAAAGG
CCGTAAAGTGAAAGTGGTTAACGGCCTGGGCAAGTTCACCGGCGCTAACACCCTGGTTGTTGAAGGCGAAAATGGCCCAA
CCACCATCAACTTCGACAATGCCATCATTGCCGCAGGTTCCCGTCCGATCCAACTGCCATTCATTCCTCATGAAGATCCA
CGCGTGTGGGACTCTACCGATGCACTGGAACTGAAAACTGTCCCAGAGCGTCTGCTGGTTATGGGCGGCGGCATCATCGG
CCTGGAAATGGGCACCGTATACCATGCGCTGGGTTCACAGATCGACGTGGTCGAAATGTTTGACCAGGTGATCCCTGCAG
CTGACAAAGACGTGGTGAAAGTCTTCACCAAACGCATCAGCAAGCAGTTCAACCTGATGCTGGAAACCAAAGTGACCGCG
GTAGAAGCCAAAGAAGACGGCATCTATGTCACTATGGAAGGCAAAAAAGCGCCTGCAGAACCACAGCGTTACGACGCGGT
GCTGGTGGCTATCGGCCGTGTGCCGAACGGCAAACTGCTGGAAGCGGGTAAAGCCGGTGTTGAAGTTGACGAGCGTGGCT
TCATCAACGTCGACAAACAACTGCGCACCAACGTGCCGCACATCTTCGCTATCGGCGACATCGTCGGTCAACCGATGCTG
GCGCACAAAGGCGTGCATGAAGGCCACGTTGCCGCAGAAGTTATCGCCGGCATGAAGCACTACTTCGACCCGAAAGTGAT
CCCATCGATCGCGTACACCGAGCCGGAAGTTGCGTGGGTGGGTCTGACCGAGAAAGAAGCGAAAGAGAAAGGCATCAGCT
ACGAAACTTCCACCTTCCCGTGGGCAGCTTCTGGCCGTGCTATCGCTTCCGATTGTGCAGACGGCATGACCAAACTGATC
TTCGACAAAGAAACTCACCGTATTATCGGTGGCGCGATTGTCGGCACCAACGGCGGCGAGCTGCTGGGTGAGATCGGTCT
GGCTATCGAGATGGGTTGCGACGCAGAAGACATCGCGCTGACTATCCATGCTCACCCAACCCTGCACGAATCCGTAGGTT
TGGCGGCTGAGATCTACGAAGGCAGCATCACCGACTTGCCTAACCCGAAAGCCAAGAAGAAGTAA

Upstream 100 bases:

>100_bases
TGCTGGCGGTGTAAGCGTCCCGGTGGATGAAGGGCGTTATGAGATCGATTAGCTATAAAAATGACGTCTGACCCGCCGGA
CAATCAATTAAGAGGTCATG

Downstream 100 bases:

>100_bases
TTCTGCTGGTTTGAACGTTTGATAAGCGGCTCCTGAAAAGGGGCCGTTTTTTTGTGCCTTCCGCTCTCAAATTTTACTAT
TCGCGACCAATAGCCATTGT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes; Glycine cleavage system L protein [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVLVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPK
TDIDKVRVWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLVVEGENGPTTINFDNAIIAAGSRPIQLPFIPHEDP
RVWDSTDALELKTVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDVVKVFTKRISKQFNLMLETKVTA
VEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKLLEAGKAGVEVDERGFINVDKQLRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETSTFPWAASGRAIASDCADGMTKLI
FDKETHRIIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK

Sequences:

>Translated_474_residues
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVLVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPK
TDIDKVRVWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLVVEGENGPTTINFDNAIIAAGSRPIQLPFIPHEDP
RVWDSTDALELKTVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDVVKVFTKRISKQFNLMLETKVTA
VEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKLLEAGKAGVEVDERGFINVDKQLRTNVPHIFAIGDIVGQPML
AHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETSTFPWAASGRAIASDCADGMTKLI
FDKETHRIIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK
>Mature_473_residues
STEIKTQVVVLGAGPAGYSAAFRCADLGLETVLVERYSTLGGVCLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKT
DIDKVRVWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFTGANTLVVEGENGPTTINFDNAIIAAGSRPIQLPFIPHEDPR
VWDSTDALELKTVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDVVKVFTKRISKQFNLMLETKVTAV
EAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKLLEAGKAGVEVDERGFINVDKQLRTNVPHIFAIGDIVGQPMLA
HKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETSTFPWAASGRAIASDCADGMTKLIF
DKETHRIIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK

Specific function: Lipoamide dehydrogenase is a component of the glycine cleavage system as well as of the alpha-ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=454, Percent_Identity=42.9515418502203, Blast_Score=338, Evalue=5e-93,
Organism=Homo sapiens, GI50301238, Length=455, Percent_Identity=27.2527472527473, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI148277065, Length=452, Percent_Identity=27.6548672566372, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI22035672, Length=429, Percent_Identity=28.4382284382284, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI33519430, Length=452, Percent_Identity=27.6548672566372, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI33519428, Length=452, Percent_Identity=27.6548672566372, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI33519426, Length=452, Percent_Identity=27.6548672566372, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI148277071, Length=452, Percent_Identity=27.6548672566372, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI291045266, Length=454, Percent_Identity=27.7533039647577, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI291045268, Length=446, Percent_Identity=26.0089686098655, Blast_Score=90, Evalue=5e-18,
Organism=Escherichia coli, GI1786307, Length=474, Percent_Identity=95.5696202531646, Blast_Score=924, Evalue=0.0,
Organism=Escherichia coli, GI87082354, Length=469, Percent_Identity=27.9317697228145, Blast_Score=184, Evalue=9e-48,
Organism=Escherichia coli, GI87081717, Length=455, Percent_Identity=28.3516483516484, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=27.9176201372998, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI32565766, Length=448, Percent_Identity=40.1785714285714, Blast_Score=318, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=27.9411764705882, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=436, Percent_Identity=26.3761467889908, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=436, Percent_Identity=26.3761467889908, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI71982272, Length=440, Percent_Identity=25.6818181818182, Blast_Score=107, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321091, Length=456, Percent_Identity=41.2280701754386, Blast_Score=305, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6325240, Length=468, Percent_Identity=26.9230769230769, Blast_Score=173, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6325166, Length=474, Percent_Identity=27.4261603375527, Blast_Score=144, Evalue=4e-35,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=40.0437636761488, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24640549, Length=462, Percent_Identity=29.004329004329, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24640553, Length=462, Percent_Identity=29.004329004329, Blast_Score=122, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24640551, Length=462, Percent_Identity=29.004329004329, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI17737741, Length=479, Percent_Identity=26.0960334029228, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50593; Mature: 50462

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVLVERYSTLGGVCLNVGCIPSKALLHVA
CCCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKALAEHGIVFGEPKTDIDKVRVWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCCC
GANTLVVEGENGPTTINFDNAIIAAGSRPIQLPFIPHEDPRVWDSTDALELKTVPERLLV
CCCEEEEECCCCCEEEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHH
MGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDVVKVFTKRISKQFNLMLETKVTA
HCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKLLEAGKAGVEVDERGFINVDKQ
EEECCCCEEEEECCCCCCCCCHHHCEEEEEEECCCCCCEEECCCCCCEECCCCCEEECHH
LRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEE
GLTEKEAKEKGISYETSTFPWAASGRAIASDCADGMTKLIFDKETHRIIGGAIVGTNGGE
ECCHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEEEEEEEECCCHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHCCCEEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
STEIKTQVVVLGAGPAGYSAAFRCADLGLETVLVERYSTLGGVCLNVGCIPSKALLHVA
CCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHH
KVIEEAKALAEHGIVFGEPKTDIDKVRVWKEKVINQLTGGLAGMAKGRKVKVVNGLGKFT
HHHHHHHHHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHCCCCEEEEEECCCCCC
GANTLVVEGENGPTTINFDNAIIAAGSRPIQLPFIPHEDPRVWDSTDALELKTVPERLLV
CCCEEEEECCCCCEEEEECCEEEECCCCEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHH
MGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDVVKVFTKRISKQFNLMLETKVTA
HCCCEEEEEHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCEEEEEEEEE
VEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPNGKLLEAGKAGVEVDERGFINVDKQ
EEECCCCEEEEECCCCCCCCCHHHCEEEEEEECCCCCCEEECCCCCCEECCCCCEEECHH
LRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEVIAGMKHYFDPKVIPSIAYTEPEVAWV
HHCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCEECCCCCEEEE
GLTEKEAKEKGISYETSTFPWAASGRAIASDCADGMTKLIFDKETHRIIGGAIVGTNGGE
ECCHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHEEEEEEEEECCCHH
LLGEIGLAIEMGCDAEDIALTIHAHPTLHESVGLAAEIYEGSITDLPNPKAKKK
HHHHCCEEEEECCCCCCEEEEEECCCCHHHHCCCEEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]