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The map label for this gene is smpB

Identifier: 157371925

GI number: 157371925

Start: 4081388

End: 4081870

Strand: Direct

Name: smpB

Synonym: Spro_3690

Alternate gene names: 157371925

Gene position: 4081388-4081870 (Clockwise)

Preceding gene: 157371922

Following gene: 157371926

Centisome position: 74.9

GC content: 51.55

Gene sequence:

>483_bases
ATGACAAAGAAAAAAGCACACAAACCCGGCTCTGCCACCATTGCGCAAAACAAGCGTGCGCGTTTCGAATACGCCATTGA
GGAAGAATTCGAAGCGGGCCTGTCTCTGCAAGGGTGGGAAGTCAAATCGCTGCGTGCCGGCAAAGCCAACATCAGTGACA
GCTACGTGACTTTTCGCGATGGTGAAGCCTACCTGTTTGGCGCCACTGTCTCACCGCTCAACGTAGCTTCTTCTCATGTG
GTCTGCGATCCGACACGTACCCGTAAACTCCTGCTGAAAAAGCGTGAGCTGGACTCACTGCTCGGCCGTGTAAATCGTGA
CGGTTACACCGTGGTTGCCTTGTCCATGTATTGGAAAAATGCCTGGGCCAAGATCAAAATCGGCGTAGCTAAAGGTAAGA
AAGAACACGATAAGCGCGATGACATCAAAGATCGTGAATGGCAGACGGCGAAATCCCGTATCATGAAGCACGCCAACCGA
TAA

Upstream 100 bases:

>100_bases
ATCTGCTATGCAACACAGAGGCCAAAAATGCGAAAAATTGCGCAGGTGCTGAGCGGGAAAGGATTTCTTTCGCTGACGCA
TCCAGTATAATGAACACACT

Downstream 100 bases:

>100_bases
GCCTCTGGCTTAACGGGATAAACTTCTGTTATACTGCGCGACGTAACTTGGGGCTGATTCTGGATTCGACGGGATTGTGA
GGCCTTAGGAGCATGCCGAG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 160; Mature: 159

Protein sequence:

>160_residues
MTKKKAHKPGSATIAQNKRARFEYAIEEEFEAGLSLQGWEVKSLRAGKANISDSYVTFRDGEAYLFGATVSPLNVASSHV
VCDPTRTRKLLLKKRELDSLLGRVNRDGYTVVALSMYWKNAWAKIKIGVAKGKKEHDKRDDIKDREWQTAKSRIMKHANR

Sequences:

>Translated_160_residues
MTKKKAHKPGSATIAQNKRARFEYAIEEEFEAGLSLQGWEVKSLRAGKANISDSYVTFRDGEAYLFGATVSPLNVASSHV
VCDPTRTRKLLLKKRELDSLLGRVNRDGYTVVALSMYWKNAWAKIKIGVAKGKKEHDKRDDIKDREWQTAKSRIMKHANR
>Mature_159_residues
TKKKAHKPGSATIAQNKRARFEYAIEEEFEAGLSLQGWEVKSLRAGKANISDSYVTFRDGEAYLFGATVSPLNVASSHVV
CDPTRTRKLLLKKRELDSLLGRVNRDGYTVVALSMYWKNAWAKIKIGVAKGKKEHDKRDDIKDREWQTAKSRIMKHANR

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family

Homologues:

Organism=Escherichia coli, GI1788973, Length=160, Percent_Identity=82.5, Blast_Score=280, Evalue=4e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SSRP_SERP5 (A8GI46)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001479914.1
- ProteinModelPortal:   A8GI46
- SMR:   A8GI46
- STRING:   A8GI46
- GeneID:   5606975
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_3690
- eggNOG:   COG0691
- HOGENOM:   HBG293422
- OMA:   EKYEAGI
- BioCyc:   SPRO399741:SPRO_3690-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00023
- InterPro:   IPR000037
- InterPro:   IPR020081
- Gene3D:   G3DSA:2.40.280.10
- ProDom:   PD004488
- TIGRFAMs:   TIGR00086

Pfam domain/function: PF01668 SmpB; SSF74982 SmpB

EC number: NA

Molecular weight: Translated: 18181; Mature: 18050

Theoretical pI: Translated: 10.62; Mature: 10.62

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKKAHKPGSATIAQNKRARFEYAIEEEFEAGLSLQGWEVKSLRAGKANISDSYVTFRD
CCCCCCCCCCCCEEECCCCCCEEHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCEEEEEC
GEAYLFGATVSPLNVASSHVVCDPTRTRKLLLKKRELDSLLGRVNRDGYTVVALSMYWKN
CCEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCC
AWAKIKIGVAKGKKEHDKRDDIKDREWQTAKSRIMKHANR
CEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TKKKAHKPGSATIAQNKRARFEYAIEEEFEAGLSLQGWEVKSLRAGKANISDSYVTFRD
CCCCCCCCCCCEEECCCCCCEEHHHHHHHHCCCCCCCCEECCCCCCCCCCCCCEEEEEC
GEAYLFGATVSPLNVASSHVVCDPTRTRKLLLKKRELDSLLGRVNRDGYTVVALSMYWKN
CCEEEEECCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCC
AWAKIKIGVAKGKKEHDKRDDIKDREWQTAKSRIMKHANR
CEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA