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The map label for this gene is pdxJ [H]

Identifier: 157371901

GI number: 157371901

Start: 4058949

End: 4059680

Strand: Reverse

Name: pdxJ [H]

Synonym: Spro_3666

Alternate gene names: 157371901

Gene position: 4059680-4058949 (Counterclockwise)

Preceding gene: 157371902

Following gene: 157371900

Centisome position: 74.51

GC content: 56.56

Gene sequence:

>732_bases
ATGGCTGATTTGCTGCTGGGCGTCAATATCGATCACATCGCCACGTTACGTAACGCGCGCGGAACCCAATACCCGGATCC
GGTTCAGGCGGCATTTATTGCCGAGCAGGCAGGGGCCGACGGGATCACCGTACACCTGCGTGAAGACCGCCGTCATATCA
CCGATCGCGATGTTCGCATTTTGCGCCAGACCATTCAGACCCGAATGAATCTGGAAATGGCCGTGACCGATGAGATGTTG
GATATCGCCATAGAGTTAAAGCCGCATTTTTGTTGTCTGGTGCCGGAAAAACGCGAAGAAGTGACCACCGAAGGTGGGCT
GGACGTCGCCGGGCAGCAAGACAAAATGGCCGTCGCGGTTGAGCAACTGGCAAAGGCCGGCATTCTGGTTTCGCTGTTTA
TCGATCCGGATCACCGCCAAATCGATGCGGCGGTCGCTGTAGGAGCACCCTATATTGAGATTCATACCGGCGCCTATGCA
GAAGCCGAAGGCGAACTGGCAGTAAAAGCCGAGCTGCGCCGCATTGCCGTTGCCGCTACCTACGCCGCAGAGAAAGGCCT
GAAGGTCAACGCCGGCCACGGCCTGACTTACCATAACGTTCAGCCAATTGCTGCTCTGCCGGAAATGCATGAGCTAAATA
TCGGCCACGCTATTATCGGCCAGGCGGTGATGTGTGGGTTACCGGCAGCGGTGACTGACATGAAATTGCTGATGCGCGAA
GCGCGTCGCTAA

Upstream 100 bases:

>100_bases
CCGGATCCGCCGGTCGACGACGCCTGACCTTTTCCCCTTTTGCAGCACTGTTCCCGCGTGTAAACTACGGCCACTGAAAA
TCGTTTTTTAGAGGGTTGTC

Downstream 100 bases:

>100_bases
TGGCAGTGCTGGGGCTGGGTACCGACATTGTCGAAATGGCGCGCATTGAAGCCGTCGTTGAGCGCAGTGGCGATCGTCTG
GCGCGCCGCGTGCTGAGCGA

Product: pyridoxine 5'-phosphate synthase

Products: NA

Alternate protein names: PNP synthase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MADLLLGVNIDHIATLRNARGTQYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRILRQTIQTRMNLEMAVTDEML
DIAIELKPHFCCLVPEKREEVTTEGGLDVAGQQDKMAVAVEQLAKAGILVSLFIDPDHRQIDAAVAVGAPYIEIHTGAYA
EAEGELAVKAELRRIAVAATYAAEKGLKVNAGHGLTYHNVQPIAALPEMHELNIGHAIIGQAVMCGLPAAVTDMKLLMRE
ARR

Sequences:

>Translated_243_residues
MADLLLGVNIDHIATLRNARGTQYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRILRQTIQTRMNLEMAVTDEML
DIAIELKPHFCCLVPEKREEVTTEGGLDVAGQQDKMAVAVEQLAKAGILVSLFIDPDHRQIDAAVAVGAPYIEIHTGAYA
EAEGELAVKAELRRIAVAATYAAEKGLKVNAGHGLTYHNVQPIAALPEMHELNIGHAIIGQAVMCGLPAAVTDMKLLMRE
ARR
>Mature_242_residues
ADLLLGVNIDHIATLRNARGTQYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRILRQTIQTRMNLEMAVTDEMLD
IAIELKPHFCCLVPEKREEVTTEGGLDVAGQQDKMAVAVEQLAKAGILVSLFIDPDHRQIDAAVAVGAPYIEIHTGAYAE
AEGELAVKAELRRIAVAATYAAEKGLKVNAGHGLTYHNVQPIAALPEMHELNIGHAIIGQAVMCGLPAAVTDMKLLMREA
RR

Specific function: Catalyzes the complicated ring closure reaction between the two acyclic compounds 1-deoxy-D-xylulose-5-phosphate (DXP) and 3-amino-2-oxopropyl phosphate (1-amino-acetone-3-phosphate or AAP) to form pyridoxine 5'-phosphate (PNP) and inorganic phosphate [H]

COG id: COG0854

COG function: function code H; Pyridoxal phosphate biosynthesis protein

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788917, Length=242, Percent_Identity=79.3388429752066, Blast_Score=382, Evalue=1e-107,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004569 [H]

Pfam domain/function: PF03740 PdxJ [H]

EC number: =2.6.99.2 [H]

Molecular weight: Translated: 26447; Mature: 26316

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLLLGVNIDHIATLRNARGTQYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRI
CCCEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
LRQTIQTRMNLEMAVTDEMLDIAIELKPHFCCLVPEKREEVTTEGGLDVAGQQDKMAVAV
HHHHHHHHHCEEEEEHHHEEEEEEEECCCEEEECCCHHHHCCCCCCCCCCCCCCHHHHHH
EQLAKAGILVSLFIDPDHRQIDAAVAVGAPYIEIHTGAYAEAEGELAVKAELRRIAVAAT
HHHHHCCEEEEEEECCCCCEEEEEEECCCCEEEEECCCEECCCCCEEHHHHHHHHHHHHH
YAAEKGLKVNAGHGLTYHNVQPIAALPEMHELNIGHAIIGQAVMCGLPAAVTDMKLLMRE
HHHHCCCEEECCCCEEECCCCCHHHCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
ARR
HCC
>Mature Secondary Structure 
ADLLLGVNIDHIATLRNARGTQYPDPVQAAFIAEQAGADGITVHLREDRRHITDRDVRI
CCEEEECCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHH
LRQTIQTRMNLEMAVTDEMLDIAIELKPHFCCLVPEKREEVTTEGGLDVAGQQDKMAVAV
HHHHHHHHHCEEEEEHHHEEEEEEEECCCEEEECCCHHHHCCCCCCCCCCCCCCHHHHHH
EQLAKAGILVSLFIDPDHRQIDAAVAVGAPYIEIHTGAYAEAEGELAVKAELRRIAVAAT
HHHHHCCEEEEEEECCCCCEEEEEEECCCCEEEEECCCEECCCCCEEHHHHHHHHHHHHH
YAAEKGLKVNAGHGLTYHNVQPIAALPEMHELNIGHAIIGQAVMCGLPAAVTDMKLLMRE
HHHHCCCEEECCCCEEECCCCCHHHCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHH
ARR
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]