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The map label for this gene is ycdJ [C]

Identifier: 157370783

GI number: 157370783

Start: 2798921

End: 2799817

Strand: Reverse

Name: ycdJ [C]

Synonym: Spro_2543

Alternate gene names: 157370783

Gene position: 2799817-2798921 (Counterclockwise)

Preceding gene: 157370784

Following gene: 157370782

Centisome position: 51.38

GC content: 59.31

Gene sequence:

>897_bases
ATGACACTGCAAAATAACGGGTCCATGTTGAATGGACCTGAACCAGAACACGTCTATACGCGGGTAAACGGCAACCGAAT
TCACTGCGTGACGCTTGGCGAAGGGCAGCCGGTATTGCTGATCCCGGGGTGGCCGCAAACCTGGTACACCTGGCGTCATG
TGATGCAGGCCCTGGCGGCGGCAGGCTTTAAGGCGATAGCGGTAGATCCGCCGGGCATTGGCGATTCAGATAAACCCCAC
GGCGGCTATGACACCGGCAGCGTGGCCGCTACCTTGCACCAGACCATGCTGCAACTGGGGCATCCGCGCTATCAACTGGT
CGGTCATGATATTGGCATGTGGGTGGGTTATGCCATGGCGAGCGATTTCCCGCAGGCGGTGACGGGGCTGGTACTGACGG
AGGCGGTGATCCCAGGGTTGGCACCCGCGCCACCGATTTTTGTACCGGCAGAACAAAACATTTTCCTGTGGCACTTCATG
TTCAACCAACTGGCGGATTTGCCTGAAGCCTTGACGCTGGGCCGCGAGAGAGAATATCTCAGCTATATCTTTAACCGCTG
GTCCTATCGCCGCGATCGGGTAGCGGCGGAAACCTACATCGCCGCCTATTCGGCACCGGGCAGCCTGCGGGCCGGTTTTG
ATTATTATCGCGCCATTCCGGAAACCATCCGGCAAAACCAGCGTCGGGCCCAGACACCGCTCAGCATGCCGGTGATGACC
ATTGGTGCCGAGCACGCCACCGGCGATGCGCCCTATGTCACGTTGCAGGGCAACGCCGTTAACCTGCGCGGCGAAACGGT
CGCTGGCTGCGGTCATTTCATCACCGAAGAGTGCCATGAGGCGTTTATCGCTCTGGTAGTGCCATTCTTGTCAGGGAGCC
TGGCCGATGCCGCTTGA

Upstream 100 bases:

>100_bases
AGGTGTTTGATGACTTTGCCGGCGCGCCGGTTGACCAACAGCAAATCAATACCTTGCTTGGCCTGCGCTGAGCCACACAA
TTCCACCACGGAGAACACCG

Downstream 100 bases:

>100_bases
CTCACAGATTGCCCGTTTTCTGGCGGAAACGACAGATGCCGCCGCGCCTGAATCCCTGAGTGAAATGCGTGCTGCCGCGC
AGGCCGGGTTGTTGGCGTTG

Product: alpha/beta hydrolase fold domain-containing protein

Products: NA

Alternate protein names: SEH; Cytosolic epoxide hydrolase; cEH; Epoxide hydratase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MTLQNNGSMLNGPEPEHVYTRVNGNRIHCVTLGEGQPVLLIPGWPQTWYTWRHVMQALAAAGFKAIAVDPPGIGDSDKPH
GGYDTGSVAATLHQTMLQLGHPRYQLVGHDIGMWVGYAMASDFPQAVTGLVLTEAVIPGLAPAPPIFVPAEQNIFLWHFM
FNQLADLPEALTLGREREYLSYIFNRWSYRRDRVAAETYIAAYSAPGSLRAGFDYYRAIPETIRQNQRRAQTPLSMPVMT
IGAEHATGDAPYVTLQGNAVNLRGETVAGCGHFITEECHEAFIALVVPFLSGSLADAA

Sequences:

>Translated_298_residues
MTLQNNGSMLNGPEPEHVYTRVNGNRIHCVTLGEGQPVLLIPGWPQTWYTWRHVMQALAAAGFKAIAVDPPGIGDSDKPH
GGYDTGSVAATLHQTMLQLGHPRYQLVGHDIGMWVGYAMASDFPQAVTGLVLTEAVIPGLAPAPPIFVPAEQNIFLWHFM
FNQLADLPEALTLGREREYLSYIFNRWSYRRDRVAAETYIAAYSAPGSLRAGFDYYRAIPETIRQNQRRAQTPLSMPVMT
IGAEHATGDAPYVTLQGNAVNLRGETVAGCGHFITEECHEAFIALVVPFLSGSLADAA
>Mature_297_residues
TLQNNGSMLNGPEPEHVYTRVNGNRIHCVTLGEGQPVLLIPGWPQTWYTWRHVMQALAAAGFKAIAVDPPGIGDSDKPHG
GYDTGSVAATLHQTMLQLGHPRYQLVGHDIGMWVGYAMASDFPQAVTGLVLTEAVIPGLAPAPPIFVPAEQNIFLWHFMF
NQLADLPEALTLGREREYLSYIFNRWSYRRDRVAAETYIAAYSAPGSLRAGFDYYRAIPETIRQNQRRAQTPLSMPVMTI
GAEHATGDAPYVTLQGNAVNLRGETVAGCGHFITEECHEAFIALVVPFLSGSLADAA

Specific function: Involved in catabolic degradation of epoxides. Shows highest activity towards C6 and C7 carbocyclic epoxides. Also active towards linear 1,2-epoxyalkanes [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm. Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Epoxide hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=230, Percent_Identity=26.5217391304348, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI27597073, Length=169, Percent_Identity=28.9940828402367, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI218777840, Length=277, Percent_Identity=23.4657039711191, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI13376164, Length=277, Percent_Identity=23.4657039711191, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17554012, Length=236, Percent_Identity=26.271186440678, Blast_Score=81, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639
- InterPro:   IPR010497 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF06441 EHN [H]

EC number: =3.3.2.10 [H]

Molecular weight: Translated: 32604; Mature: 32473

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLQNNGSMLNGPEPEHVYTRVNGNRIHCVTLGEGQPVLLIPGWPQTWYTWRHVMQALAA
CEECCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHH
AGFKAIAVDPPGIGDSDKPHGGYDTGSVAATLHQTMLQLGHPRYQLVGHDIGMWVGYAMA
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
SDFPQAVTGLVLTEAVIPGLAPAPPIFVPAEQNIFLWHFMFNQLADLPEALTLGREREYL
CCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCEEEHHHHHHHHHCCHHHHHCCCHHHHH
SYIFNRWSYRRDRVAAETYIAAYSAPGSLRAGFDYYRAIPETIRQNQRRAQTPLSMPVMT
HHHHHHHHHHHHHHHHHHHHEEECCCCCCHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
IGAEHATGDAPYVTLQGNAVNLRGETVAGCGHFITEECHEAFIALVVPFLSGSLADAA
ECCCCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TLQNNGSMLNGPEPEHVYTRVNGNRIHCVTLGEGQPVLLIPGWPQTWYTWRHVMQALAA
EECCCCCCCCCCCCCEEEEEECCCEEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHH
AGFKAIAVDPPGIGDSDKPHGGYDTGSVAATLHQTMLQLGHPRYQLVGHDIGMWVGYAMA
CCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHH
SDFPQAVTGLVLTEAVIPGLAPAPPIFVPAEQNIFLWHFMFNQLADLPEALTLGREREYL
CCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCEEEHHHHHHHHHCCHHHHHCCCHHHHH
SYIFNRWSYRRDRVAAETYIAAYSAPGSLRAGFDYYRAIPETIRQNQRRAQTPLSMPVMT
HHHHHHHHHHHHHHHHHHHHEEECCCCCCHHCHHHHHHHHHHHHHHHHHHCCCCCCCEEE
IGAEHATGDAPYVTLQGNAVNLRGETVAGCGHFITEECHEAFIALVVPFLSGSLADAA
ECCCCCCCCCCEEEEECCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9578475 [H]