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The map label for this gene is emrB [C]

Identifier: 157370720

GI number: 157370720

Start: 2720436

End: 2721950

Strand: Reverse

Name: emrB [C]

Synonym: Spro_2480

Alternate gene names: 157370720

Gene position: 2721950-2720436 (Counterclockwise)

Preceding gene: 157370722

Following gene: 157370719

Centisome position: 49.95

GC content: 57.56

Gene sequence:

>1515_bases
ATGGGTATTGATAACGTAGCTGGCAACGAAGAAAGCGGCAGGGAGTCTGAACAGGCACCCTCGATCCGCTTGCTGTTCTC
CGCGCTGCTGCTGGTTATGCTGCTGGCAGCACTCGATCAGACCATTGTGTCTACCGCATTACCGACCATCGTTGGCGAAT
TTGGTGGCCTGGAGAACCTGTCGTGGGTGGTGACCGCCTATCTGTTGGCATCCACCATCGTGGTGCCGCTGTATGGCAAG
TTTGGCGATCTGTTCGGTCGCAAGATAGTGCTGCAGATCGCCATTGTGGTGTTCCTGCTCGGTTCGGTGCTGTGTGGGTT
GGCACAGAATATGACGGAACTTATCCTGATGCGTGCGCTGCAAGGGCTGGGCGGCGGGGGTCTGTTGGTGGTGACCATGG
CGGTGGTCGGCGATGTGATCCCACCGGCCGATCGTGGACGCTATCAAGGGTTGTTTGGCGGGGTATTTGGTCTGGCGACG
GTGGTCGGCCCGCTGATCGGCGGGTTGCTGGTGCAGCATTTCTCCTGGCGCTGGATTTTCTATATCAACCTGCCGCTGGG
GATTTTCGCGCTATTGGTGATTGGCGCGGTGTTCCAGTCGAAGGTCAATCGGGTTAAACATGAGATTGATTTTCTCGGGG
CCAGTTATCTCGCGGCGGCACTGACCTGCATCATCCTGTTTACCAGCCAGGGCGGTACGGTGATGGCCTGGTCGGACGGT
CAACTGTGGTGCATTCTGGCCTTTGGCCTGGTGTCGATAGGCGGGTTTATCTACGAAGAGCGGCTGGCGAGCGAGCCGAT
TATTCCGCTCGACCTGTTCCGTAATCGCACCTTTGTACTCTGTGCATTGATAGGTTTTATCGTCGGTATGTCACTGTTTG
GATCGGTGACTTTTCTGCCGCTGTATCTGCAGGTAGTGAAGGATTCCACGCCTACCCAGGCCGGGATGCAGCTGCTGCCG
CTGATGGGTGGGATTATGCTCAGTTCGATCATCAGCGGTCGGTTGATCAGCAAGCGCGGCAAATACCGCATTTTCCCGAT
TATCGGCACCCTGTGTGCCTTGGCAGGCATGGTGTTGCTGGGAACGTTGAAAAACTCGACCCCGGTGCATTTGCTGTACC
TCTACATCAGCCTGCTGGGGTTGGGGCTGGGTATGGTGATGCAGGTGATGATCCTGGCGGCGCAAAACAGCGTAGAGCCA
AAGCATATGGGGGTAGCGACCTCCAGCGCCACGCTGTTCCGCTCTATTGGCGGCTCTATCGGCGTGGCGGTGTTTGGCGC
AATTTTCACCAATGTGCTGCACAGCCGCCTGGCCACTTTGCTGCCGGAGGGCGCAGAGCTGCCGCGTTCACTGGGGGCTG
ATGCCGTGCACCATTTGCCGGTGCAACTGCAAAATGACTACCTGCAGGCCTTCGGCGGTGCGATCCACTCGGTGTACCAG
ATTGCGGCGGGAGTAATGGCGCTGGCCTTTGCCTTGACCTGGCTGTTGAAAGACGTGCCTCTGCGCAAGAAATAA

Upstream 100 bases:

>100_bases
AACGTTTTGTGCTGTCATCGCGGTGAGATCCATTGACCGGAGAAAAGAATAACCCATATATTAATTACTCGATTAATTAA
TTACAAGCAGGCTCGGCTAA

Downstream 100 bases:

>100_bases
ACCCTGAGCCGGTGCGCGAAATGCGTGCGTACCGGCTCACCACTCGCTCTGCAGTGCACTTTTACTGAATCCTTACGGAA
CCCTGTCAGACTAAAAACGT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 504; Mature: 503

Protein sequence:

>504_residues
MGIDNVAGNEESGRESEQAPSIRLLFSALLLVMLLAALDQTIVSTALPTIVGEFGGLENLSWVVTAYLLASTIVVPLYGK
FGDLFGRKIVLQIAIVVFLLGSVLCGLAQNMTELILMRALQGLGGGGLLVVTMAVVGDVIPPADRGRYQGLFGGVFGLAT
VVGPLIGGLLVQHFSWRWIFYINLPLGIFALLVIGAVFQSKVNRVKHEIDFLGASYLAAALTCIILFTSQGGTVMAWSDG
QLWCILAFGLVSIGGFIYEERLASEPIIPLDLFRNRTFVLCALIGFIVGMSLFGSVTFLPLYLQVVKDSTPTQAGMQLLP
LMGGIMLSSIISGRLISKRGKYRIFPIIGTLCALAGMVLLGTLKNSTPVHLLYLYISLLGLGLGMVMQVMILAAQNSVEP
KHMGVATSSATLFRSIGGSIGVAVFGAIFTNVLHSRLATLLPEGAELPRSLGADAVHHLPVQLQNDYLQAFGGAIHSVYQ
IAAGVMALAFALTWLLKDVPLRKK

Sequences:

>Translated_504_residues
MGIDNVAGNEESGRESEQAPSIRLLFSALLLVMLLAALDQTIVSTALPTIVGEFGGLENLSWVVTAYLLASTIVVPLYGK
FGDLFGRKIVLQIAIVVFLLGSVLCGLAQNMTELILMRALQGLGGGGLLVVTMAVVGDVIPPADRGRYQGLFGGVFGLAT
VVGPLIGGLLVQHFSWRWIFYINLPLGIFALLVIGAVFQSKVNRVKHEIDFLGASYLAAALTCIILFTSQGGTVMAWSDG
QLWCILAFGLVSIGGFIYEERLASEPIIPLDLFRNRTFVLCALIGFIVGMSLFGSVTFLPLYLQVVKDSTPTQAGMQLLP
LMGGIMLSSIISGRLISKRGKYRIFPIIGTLCALAGMVLLGTLKNSTPVHLLYLYISLLGLGLGMVMQVMILAAQNSVEP
KHMGVATSSATLFRSIGGSIGVAVFGAIFTNVLHSRLATLLPEGAELPRSLGADAVHHLPVQLQNDYLQAFGGAIHSVYQ
IAAGVMALAFALTWLLKDVPLRKK
>Mature_503_residues
GIDNVAGNEESGRESEQAPSIRLLFSALLLVMLLAALDQTIVSTALPTIVGEFGGLENLSWVVTAYLLASTIVVPLYGKF
GDLFGRKIVLQIAIVVFLLGSVLCGLAQNMTELILMRALQGLGGGGLLVVTMAVVGDVIPPADRGRYQGLFGGVFGLATV
VGPLIGGLLVQHFSWRWIFYINLPLGIFALLVIGAVFQSKVNRVKHEIDFLGASYLAAALTCIILFTSQGGTVMAWSDGQ
LWCILAFGLVSIGGFIYEERLASEPIIPLDLFRNRTFVLCALIGFIVGMSLFGSVTFLPLYLQVVKDSTPTQAGMQLLPL
MGGIMLSSIISGRLISKRGKYRIFPIIGTLCALAGMVLLGTLKNSTPVHLLYLYISLLGLGLGMVMQVMILAAQNSVEPK
HMGVATSSATLFRSIGGSIGVAVFGAIFTNVLHSRLATLLPEGAELPRSLGADAVHHLPVQLQNDYLQAFGGAIHSVYQI
AAGVMALAFALTWLLKDVPLRKK

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=407, Percent_Identity=25.7985257985258, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI87081983, Length=310, Percent_Identity=31.2903225806452, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1790195, Length=411, Percent_Identity=27.4939172749392, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788710, Length=467, Percent_Identity=22.2698072805139, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788392, Length=323, Percent_Identity=30.030959752322, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790146, Length=173, Percent_Identity=30.635838150289, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87082231, Length=187, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994889, Length=104, Percent_Identity=34.6153846153846, Blast_Score=63, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=551, Percent_Identity=24.1379310344828, Blast_Score=158, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6319770, Length=468, Percent_Identity=28.6324786324786, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6325455, Length=509, Percent_Identity=25.5402750491159, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323735, Length=465, Percent_Identity=27.5268817204301, Blast_Score=141, Evalue=3e-34,
Organism=Saccharomyces cerevisiae, GI6321663, Length=511, Percent_Identity=22.8962818003914, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319783, Length=438, Percent_Identity=21.9178082191781, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324954, Length=410, Percent_Identity=24.6341463414634, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320770, Length=488, Percent_Identity=21.7213114754098, Blast_Score=67, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6322070, Length=103, Percent_Identity=33.9805825242718, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6322071, Length=160, Percent_Identity=27.5, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323520, Length=383, Percent_Identity=24.5430809399478, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53733; Mature: 53602

Theoretical pI: Translated: 8.67; Mature: 8.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIDNVAGNEESGRESEQAPSIRLLFSALLLVMLLAALDQTIVSTALPTIVGEFGGLENL
CCCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
SWVVTAYLLASTIVVPLYGKFGDLFGRKIVLQIAIVVFLLGSVLCGLAQNMTELILMRAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGLGGGGLLVVTMAVVGDVIPPADRGRYQGLFGGVFGLATVVGPLIGGLLVQHFSWRWIF
HCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
YINLPLGIFALLVIGAVFQSKVNRVKHEIDFLGASYLAAALTCIILFTSQGGTVMAWSDG
EEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCC
QLWCILAFGLVSIGGFIYEERLASEPIIPLDLFRNRTFVLCALIGFIVGMSLFGSVTFLP
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LYLQVVKDSTPTQAGMQLLPLMGGIMLSSIISGRLISKRGKYRIFPIIGTLCALAGMVLL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHCCCCEEEHHHHHHHHHHHHHHHH
GTLKNSTPVHLLYLYISLLGLGLGMVMQVMILAAQNSVEPKHMGVATSSATLFRSIGGSI
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHHCCHH
GVAVFGAIFTNVLHSRLATLLPEGAELPRSLGADAVHHLPVQLQNDYLQAFGGAIHSVYQ
HHHHHHHHHHHHHHHHHHHHCCCCHHCHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHH
IAAGVMALAFALTWLLKDVPLRKK
HHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GIDNVAGNEESGRESEQAPSIRLLFSALLLVMLLAALDQTIVSTALPTIVGEFGGLENL
CCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH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HHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]