Click here to switch to the map view.

The map label for this gene is mexA [H]

Identifier: 157370719

GI number: 157370719

Start: 2719133

End: 2720287

Strand: Reverse

Name: mexA [H]

Synonym: Spro_2479

Alternate gene names: 157370719

Gene position: 2720287-2719133 (Counterclockwise)

Preceding gene: 157370720

Following gene: 157370718

Centisome position: 49.92

GC content: 59.39

Gene sequence:

>1155_bases
ATGCAAAGGAACAAGGTTTTACTGGTCAGCCTGTCGCTGTGGTTGTCTGCCTGTGATGGTCAGTCCGGCAGTACACCGGC
TGGTGCGGTCGGCGCACCTGAGGTTGGCGTGGCAACGTTGCGCAGCCAGCCGGTCACGCTGAGCAGTGACCTGACGGGCC
GGGTCAATGCCACCATGACCTCGGATGTTCGTCCGCAGGTGGAAGGCATTATCAAGGCACGCCTGTTTACCGAAGGGGCG
GAGGTCAAAGCCGGACAGGTGCTGTATCAAATCGATCCCGCGACCTACCAGGCCAGTTACGATCAGGCCGCCGCGCAGTT
GAAAAGCGCCCAGGCCACGGTGAAGTCCAGCCGCTTGAAGGCGCAGCGCTATGCCGCACTGGTGAAGGAGAATGGCGTCT
CCAAACAGGATGCGGATGATGCGATGGCAACCTATCTGGAGGCCGTAGCCGCCGTCGCGCAGTATCAGGCGGCGCTGGAA
ACCACCAAAATCAATCTTGCCTATACCCAGGTACGCGCACCGATTTCCGGCCGTATTGGTATTTCTTCGGTCACGCCGGG
GGCGCTGGTGACCGCCAGCCAAACCGATGCACTGGCAACCATTCGCGCGCTCGATCCTGTCTATGTCGATCTCACCCAGT
CCAGCAGTCAACTGCTCAAATTGCGCAAGCAGCAGGCTTCACTACAACGTGACGATGTCACGCCGGTAGCGATCAAACTC
GAAGATGGCACGCCCTATACCCATCCCGGCAAGCTGGAACTGACCGAAGTGTCGGTGGATGAATCTACCGGCTCTGTCAC
GCTGCGTGCCGTGTTCCCTAACCCCGAGCACGAATTGCTACCGGGCATGTATGTCCATGCCACCCTGGACAACGGGGTCG
ATCCCAAAGGCATTTTGGCACCGCAACAGGGCATTACCCGCAATGCCAGGGGCGAGGCGACCGCGTTGGTGGTTAACGCG
CAAAACAAAGTGGAACAACGCACCGTCACCACCGGACAGGTGATCGGCAGCAGTTGGCTGGTCAGCAAAGGGCTGAATGC
AGGCGACCGACTGATTGTCGAAGGCACCAGTAAGGTGGCCGTCGGCGTTGAGGTCAAGGCCGTTGAAGTGCAACAGCAGG
CGAATAAGGCTGCGACTAACGGCGGGGATCAATAA

Upstream 100 bases:

>100_bases
CTGCAGTGCACTTTTACTGAATCCTTACGGAACCCTGTCAGACTAAAAACGTCGCTTTCTATATATTGAGAATAAACGTT
CTCAATAGGATGAAAAGTCG

Downstream 100 bases:

>100_bases
CCATGGCGCAGTTCTTTATTCGGCGGCCGGTGTTTGCCTGGGTGATTGCGATCATCATCATGCTGTGTGGCCTGATGTCG
ATCAATTCGCTCCCCATCTC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MQRNKVLLVSLSLWLSACDGQSGSTPAGAVGAPEVGVATLRSQPVTLSSDLTGRVNATMTSDVRPQVEGIIKARLFTEGA
EVKAGQVLYQIDPATYQASYDQAAAQLKSAQATVKSSRLKAQRYAALVKENGVSKQDADDAMATYLEAVAAVAQYQAALE
TTKINLAYTQVRAPISGRIGISSVTPGALVTASQTDALATIRALDPVYVDLTQSSSQLLKLRKQQASLQRDDVTPVAIKL
EDGTPYTHPGKLELTEVSVDESTGSVTLRAVFPNPEHELLPGMYVHATLDNGVDPKGILAPQQGITRNARGEATALVVNA
QNKVEQRTVTTGQVIGSSWLVSKGLNAGDRLIVEGTSKVAVGVEVKAVEVQQQANKAATNGGDQ

Sequences:

>Translated_384_residues
MQRNKVLLVSLSLWLSACDGQSGSTPAGAVGAPEVGVATLRSQPVTLSSDLTGRVNATMTSDVRPQVEGIIKARLFTEGA
EVKAGQVLYQIDPATYQASYDQAAAQLKSAQATVKSSRLKAQRYAALVKENGVSKQDADDAMATYLEAVAAVAQYQAALE
TTKINLAYTQVRAPISGRIGISSVTPGALVTASQTDALATIRALDPVYVDLTQSSSQLLKLRKQQASLQRDDVTPVAIKL
EDGTPYTHPGKLELTEVSVDESTGSVTLRAVFPNPEHELLPGMYVHATLDNGVDPKGILAPQQGITRNARGEATALVVNA
QNKVEQRTVTTGQVIGSSWLVSKGLNAGDRLIVEGTSKVAVGVEVKAVEVQQQANKAATNGGDQ
>Mature_384_residues
MQRNKVLLVSLSLWLSACDGQSGSTPAGAVGAPEVGVATLRSQPVTLSSDLTGRVNATMTSDVRPQVEGIIKARLFTEGA
EVKAGQVLYQIDPATYQASYDQAAAQLKSAQATVKSSRLKAQRYAALVKENGVSKQDADDAMATYLEAVAAVAQYQAALE
TTKINLAYTQVRAPISGRIGISSVTPGALVTASQTDALATIRALDPVYVDLTQSSSQLLKLRKQQASLQRDDVTPVAIKL
EDGTPYTHPGKLELTEVSVDESTGSVTLRAVFPNPEHELLPGMYVHATLDNGVDPKGILAPQQGITRNARGEATALVVNA
QNKVEQRTVTTGQVIGSSWLVSKGLNAGDRLIVEGTSKVAVGVEVKAVEVQQQANKAATNGGDQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=379, Percent_Identity=48.8126649076517, Blast_Score=342, Evalue=2e-95,
Organism=Escherichia coli, GI1789665, Length=365, Percent_Identity=48.2191780821918, Blast_Score=334, Evalue=7e-93,
Organism=Escherichia coli, GI1789929, Length=379, Percent_Identity=44.8548812664908, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI87082036, Length=321, Percent_Identity=27.4143302180685, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI87081796, Length=339, Percent_Identity=26.2536873156342, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 40402; Mature: 40402

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRNKVLLVSLSLWLSACDGQSGSTPAGAVGAPEVGVATLRSQPVTLSSDLTGRVNATMT
CCCCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCEEECCCCCCEEEEEEE
SDVRPQVEGIIKARLFTEGAEVKAGQVLYQIDPATYQASYDQAAAQLKSAQATVKSSRLK
CCCCHHHHHHHHHHEECCCCCEECCEEEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHH
AQRYAALVKENGVSKQDADDAMATYLEAVAAVAQYQAALETTKINLAYTQVRAPISGRIG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCC
ISSVTPGALVTASQTDALATIRALDPVYVDLTQSSSQLLKLRKQQASLQRDDVTPVAIKL
CCCCCCCEEEEECCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEE
EDGTPYTHPGKLELTEVSVDESTGSVTLRAVFPNPEHELLPGMYVHATLDNGVDPKGILA
ECCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCHHHCCCEEEEEEECCCCCCCCEEC
PQQGITRNARGEATALVVNAQNKVEQRTVTTGQVIGSSWLVSKGLNAGDRLIVEGTSKVA
CCCCCCCCCCCCEEEEEEECCHHHHHHHCCHHHHHCCCHHHHCCCCCCCEEEEECCCEEE
VGVEVKAVEVQQQANKAATNGGDQ
EEEEEEEEHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MQRNKVLLVSLSLWLSACDGQSGSTPAGAVGAPEVGVATLRSQPVTLSSDLTGRVNATMT
CCCCCEEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCHHHHCCCCEEECCCCCCEEEEEEE
SDVRPQVEGIIKARLFTEGAEVKAGQVLYQIDPATYQASYDQAAAQLKSAQATVKSSRLK
CCCCHHHHHHHHHHEECCCCCEECCEEEEEECCCCEECHHHHHHHHHHHHHHHHHHHHHH
AQRYAALVKENGVSKQDADDAMATYLEAVAAVAQYQAALETTKINLAYTQVRAPISGRIG
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCC
ISSVTPGALVTASQTDALATIRALDPVYVDLTQSSSQLLKLRKQQASLQRDDVTPVAIKL
CCCCCCCEEEEECCCCHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEE
EDGTPYTHPGKLELTEVSVDESTGSVTLRAVFPNPEHELLPGMYVHATLDNGVDPKGILA
ECCCCCCCCCEEEEEEEEECCCCCCEEEEEECCCCCHHHCCCEEEEEEECCCCCCCCEEC
PQQGITRNARGEATALVVNAQNKVEQRTVTTGQVIGSSWLVSKGLNAGDRLIVEGTSKVA
CCCCCCCCCCCCEEEEEEECCHHHHHHHCCHHHHHCCCHHHHCCCCCCCEEEEECCCEEE
VGVEVKAVEVQQQANKAATNGGDQ
EEEEEEEEHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]