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The map label for this gene is gatA [H]

Identifier: 157369661

GI number: 157369661

Start: 1546174

End: 1548003

Strand: Direct

Name: gatA [H]

Synonym: Spro_1418

Alternate gene names: 157369661

Gene position: 1546174-1548003 (Clockwise)

Preceding gene: 157369657

Following gene: 157369662

Centisome position: 28.38

GC content: 63.33

Gene sequence:

>1830_bases
ATGACTCAGTCCGTTAGCCTACGCGGTTTCACCCTCAGCGAATGGCAGCATCATTATCATACCCACCCGGCCGGCGAGCG
CATGGCCTGTGTGCGTACCACGCTGGTTGCCTTGCTGGCCAATTTGCCAGCGGATGACAATGCCTGGCTGTACCTTGCCA
GCGCTGAACAACTGGAGGGGCAGTACCAACAACTGGCCCAACGGCTTGAAGCCGTCGCCGGGGACATCAGCCGCCTGCCG
CTGTTTGGTGTGCCTTTTGCGGTCAAAGACAATATCGATGTCGGCGGTTGGCCGACTACCGCCGCCTGCCCGGAGTTTGC
CTATCAGGCGACGGCAGATGCAGCCGTCGTCGCCAATCTGCGCGCCGCCGGTGCGCTGGTGATCGGTAAAACCAATCTCG
ATCAGTTCGCTACCGGCCTGGTCGGCACCCGTTCGCCGCACGGCGCAGTGGTGAACAGTTTCGACGGTCGCTATGTCAGC
GGCGGCTCCAGCTCCGGCTCGGCCTCGGTGGTGGCGCGCGGTCTGACGCCGTTCTCATTGGGAACCGATACCGCCGGTTC
AGGGCGCGTCCCGGCCGGATTCAACAATATTGTCGGCCTGAAACCGACCAAGGGCTGGCTGTCGAACCGTGGCGTGGTAC
CCGCCTGTCGGCTCAATGACACGGTGTCGGTATTCGCCCTGACGGTGGCAGACGCTGCGCTGGTGGCCGAACTGGCCGGC
GGCTATGACGCTGCCGATGCCTATTCCCGCATCAATCCCCATACCGCCCCGGCGAGTCTTCCCGCGGCCCCCCGCTTTGC
GGTACCGGCGCAGCTCGAGTTTTTCGGCGATACCCAGGCCGAGCAGGCATTTCAACGGGCGCTGGAGCAGTTGCAAAGCT
GCGGCGTAACGCTGGAAACCCTCGATTTTACCCCGTTCCGCGTTCTGGCAGATCAGCTCTATTACGGCCCCTGGGTGGCG
GAACGCACCGTGGCGGTAGAGAAGATATTGAGTGCCAATCCACAGGCGATTAATCCGGTGGTGCGCGGCATCGTCAATAA
CGGCCTGAAGTACAGCGCCTGTGACGCCTACAAAGCGGAATATCTGCGTGCCGAGCTGGCGCGGCAGATTGAACAACGGC
TGGCTCCCTTCGACGCACTGGTGGTGCCGACCTCTCCGACCATTCGCACACTGGCGGAAATGGAGCAGGAGCCGGTGTTG
TTCAACTCGCAGTTTGGCACCTACACCAATTTCACCAACCTGGCCGATCTGAGCGCGCTGGCACTGCCTGCGCCGATGCG
CGACGACGGCTTGCCTGCCGGTATCACGCTGGTTGCTCCGGCCTGGCACGATCGGGCGCTGGCGGCCTTCGGTCTGCGCT
GGCAACAGGCTCAGGCTCTGCCGTTGGGTGCGACCGGTCGCGTGCTACCGCCGCTGAATTCTCTGACGCCATCGGCTCAG
CACGTGCAGGTAGCGGTAGTCGGCGCTCACCTCAGCGGGATGCCGCTGAATTTCCAGCTCACTCAGCGTGCCGCGATTTT
GCTGGAGCAAACCACCACCGCACCCTGCTACCGGCTGTACGCGCTGGCCAATACTCAGCCGCCAAAGCCGGGGCTGGTCA
AAACCGAGCAGGGGGCCGCAATCCGTGTGGAACTGTGGGAGATCCCGCTGGCGCGTTTTGGCGAATTGGTGGCGGAGATC
CCGGCGCCGCTGGGTATCGGCACGCTGTTGCTGGCGGATGGTCGCCGGGTGAAAGGCTTTATCTGCGAACCCTGGGCGCT
GCAGGGGGCCACGGATATCACTGAATTTGGCGGCTGGCGCAATTATGTCGCCAGCCTGAAGGGGGCTTGA

Upstream 100 bases:

>100_bases
CTATAAGTTTTTCTTATATTTTATTGAAATCAGCCGATTAAAAATTGGCACGCCGGTTGCTCTATGAATCCCATCTTGTG
TACCAGATGGAATTTTGCCA

Downstream 100 bases:

>100_bases
TCATGTTTTCTACCGTACTGATTGCCAACCGTGGCGAAATTGCCTGCCGCGCCATCCGCACCCTGAAACGCCTGGGAGTG
ACCAGCGTGGCGGTTTATTC

Product: allophanate hydrolase

Products: monocarboxylate; NH3

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 609; Mature: 608

Protein sequence:

>609_residues
MTQSVSLRGFTLSEWQHHYHTHPAGERMACVRTTLVALLANLPADDNAWLYLASAEQLEGQYQQLAQRLEAVAGDISRLP
LFGVPFAVKDNIDVGGWPTTAACPEFAYQATADAAVVANLRAAGALVIGKTNLDQFATGLVGTRSPHGAVVNSFDGRYVS
GGSSSGSASVVARGLTPFSLGTDTAGSGRVPAGFNNIVGLKPTKGWLSNRGVVPACRLNDTVSVFALTVADAALVAELAG
GYDAADAYSRINPHTAPASLPAAPRFAVPAQLEFFGDTQAEQAFQRALEQLQSCGVTLETLDFTPFRVLADQLYYGPWVA
ERTVAVEKILSANPQAINPVVRGIVNNGLKYSACDAYKAEYLRAELARQIEQRLAPFDALVVPTSPTIRTLAEMEQEPVL
FNSQFGTYTNFTNLADLSALALPAPMRDDGLPAGITLVAPAWHDRALAAFGLRWQQAQALPLGATGRVLPPLNSLTPSAQ
HVQVAVVGAHLSGMPLNFQLTQRAAILLEQTTTAPCYRLYALANTQPPKPGLVKTEQGAAIRVELWEIPLARFGELVAEI
PAPLGIGTLLLADGRRVKGFICEPWALQGATDITEFGGWRNYVASLKGA

Sequences:

>Translated_609_residues
MTQSVSLRGFTLSEWQHHYHTHPAGERMACVRTTLVALLANLPADDNAWLYLASAEQLEGQYQQLAQRLEAVAGDISRLP
LFGVPFAVKDNIDVGGWPTTAACPEFAYQATADAAVVANLRAAGALVIGKTNLDQFATGLVGTRSPHGAVVNSFDGRYVS
GGSSSGSASVVARGLTPFSLGTDTAGSGRVPAGFNNIVGLKPTKGWLSNRGVVPACRLNDTVSVFALTVADAALVAELAG
GYDAADAYSRINPHTAPASLPAAPRFAVPAQLEFFGDTQAEQAFQRALEQLQSCGVTLETLDFTPFRVLADQLYYGPWVA
ERTVAVEKILSANPQAINPVVRGIVNNGLKYSACDAYKAEYLRAELARQIEQRLAPFDALVVPTSPTIRTLAEMEQEPVL
FNSQFGTYTNFTNLADLSALALPAPMRDDGLPAGITLVAPAWHDRALAAFGLRWQQAQALPLGATGRVLPPLNSLTPSAQ
HVQVAVVGAHLSGMPLNFQLTQRAAILLEQTTTAPCYRLYALANTQPPKPGLVKTEQGAAIRVELWEIPLARFGELVAEI
PAPLGIGTLLLADGRRVKGFICEPWALQGATDITEFGGWRNYVASLKGA
>Mature_608_residues
TQSVSLRGFTLSEWQHHYHTHPAGERMACVRTTLVALLANLPADDNAWLYLASAEQLEGQYQQLAQRLEAVAGDISRLPL
FGVPFAVKDNIDVGGWPTTAACPEFAYQATADAAVVANLRAAGALVIGKTNLDQFATGLVGTRSPHGAVVNSFDGRYVSG
GSSSGSASVVARGLTPFSLGTDTAGSGRVPAGFNNIVGLKPTKGWLSNRGVVPACRLNDTVSVFALTVADAALVAELAGG
YDAADAYSRINPHTAPASLPAAPRFAVPAQLEFFGDTQAEQAFQRALEQLQSCGVTLETLDFTPFRVLADQLYYGPWVAE
RTVAVEKILSANPQAINPVVRGIVNNGLKYSACDAYKAEYLRAELARQIEQRLAPFDALVVPTSPTIRTLAEMEQEPVLF
NSQFGTYTNFTNLADLSALALPAPMRDDGLPAGITLVAPAWHDRALAAFGLRWQQAQALPLGATGRVLPPLNSLTPSAQH
VQVAVVGAHLSGMPLNFQLTQRAAILLEQTTTAPCYRLYALANTQPPKPGLVKTEQGAAIRVELWEIPLARFGELVAEIP
APLGIGTLLLADGRRVKGFICEPWALQGATDITEFGGWRNYVASLKGA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=461, Percent_Identity=26.6811279826464, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI195972892, Length=153, Percent_Identity=33.9869281045752, Blast_Score=87, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17543272, Length=400, Percent_Identity=30.75, Blast_Score=140, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17556264, Length=196, Percent_Identity=32.6530612244898, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17537465, Length=446, Percent_Identity=24.8878923766816, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=133, Percent_Identity=33.0827067669173, Blast_Score=81, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71990152, Length=141, Percent_Identity=32.6241134751773, Blast_Score=69, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=627, Percent_Identity=44.0191387559809, Blast_Score=444, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6323950, Length=173, Percent_Identity=38.728323699422, Blast_Score=94, Evalue=8e-20,
Organism=Drosophila melanogaster, GI24648113, Length=447, Percent_Identity=26.8456375838926, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI21356731, Length=132, Percent_Identity=37.1212121212121, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24652985, Length=137, Percent_Identity=37.956204379562, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922090, Length=137, Percent_Identity=37.956204379562, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652981, Length=137, Percent_Identity=37.956204379562, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24652983, Length=137, Percent_Identity=37.956204379562, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24644968, Length=413, Percent_Identity=24.6973365617433, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI45550774, Length=139, Percent_Identity=32.3741007194245, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648435, Length=139, Percent_Identity=32.3741007194245, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648437, Length=139, Percent_Identity=32.3741007194245, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24648441, Length=139, Percent_Identity=32.3741007194245, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24648439, Length=139, Percent_Identity=32.3741007194245, Blast_Score=67, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 3.5.1.4

Molecular weight: Translated: 64833; Mature: 64702

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQSVSLRGFTLSEWQHHYHTHPAGERMACVRTTLVALLANLPADDNAWLYLASAEQLEG
CCCCEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHH
QYQQLAQRLEAVAGDISRLPLFGVPFAVKDNIDVGGWPTTAACPEFAYQATADAAVVANL
HHHHHHHHHHHHHCCHHCCCEECCCEEEECCCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
RAAGALVIGKTNLDQFATGLVGTRSPHGAVVNSFDGRYVSGGSSSGSASVVARGLTPFSL
HHCCEEEEECCCHHHHHHCCCCCCCCCCCEEECCCCEEECCCCCCCCCCEEEECCCCCCC
GTDTAGSGRVPAGFNNIVGLKPTKGWLSNRGVVPACRLNDTVSVFALTVADAALVAELAG
CCCCCCCCCCCCCCCCCEECCCCCCHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHC
GYDAADAYSRINPHTAPASLPAAPRFAVPAQLEFFGDTQAEQAFQRALEQLQSCGVTLET
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEE
LDFTPFRVLADQLYYGPWVAERTVAVEKILSANPQAINPVVRGIVNNGLKYSACDAYKAE
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
YLRAELARQIEQRLAPFDALVVPTSPTIRTLAEMEQEPVLFNSQFGTYTNFTNLADLSAL
HHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHH
ALPAPMRDDGLPAGITLVAPAWHDRALAAFGLRWQQAQALPLGATGRVLPPLNSLTPSAQ
HCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCCCCCC
HVQVAVVGAHLSGMPLNFQLTQRAAILLEQTTTAPCYRLYALANTQPPKPGLVKTEQGAA
EEEEEEEEECCCCCCCEEEEHHHEEEEEECCCCCCCEEEEEECCCCCCCCCCEECCCCCE
IRVELWEIPLARFGELVAEIPAPLGIGTLLLADGRRVKGFICEPWALQGATDITEFGGWR
EEEEEECCCHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCHHHHHCCHH
NYVASLKGA
HHHHHHCCC
>Mature Secondary Structure 
TQSVSLRGFTLSEWQHHYHTHPAGERMACVRTTLVALLANLPADDNAWLYLASAEQLEG
CCCEEECCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECHHHHHH
QYQQLAQRLEAVAGDISRLPLFGVPFAVKDNIDVGGWPTTAACPEFAYQATADAAVVANL
HHHHHHHHHHHHHCCHHCCCEECCCEEEECCCCCCCCCCCCCCHHHHHHHCCCHHHHHHH
RAAGALVIGKTNLDQFATGLVGTRSPHGAVVNSFDGRYVSGGSSSGSASVVARGLTPFSL
HHCCEEEEECCCHHHHHHCCCCCCCCCCCEEECCCCEEECCCCCCCCCCEEEECCCCCCC
GTDTAGSGRVPAGFNNIVGLKPTKGWLSNRGVVPACRLNDTVSVFALTVADAALVAELAG
CCCCCCCCCCCCCCCCCEECCCCCCHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHC
GYDAADAYSRINPHTAPASLPAAPRFAVPAQLEFFGDTQAEQAFQRALEQLQSCGVTLET
CCCHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHCCCEEEE
LDFTPFRVLADQLYYGPWVAERTVAVEKILSANPQAINPVVRGIVNNGLKYSACDAYKAE
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHH
YLRAELARQIEQRLAPFDALVVPTSPTIRTLAEMEQEPVLFNSQFGTYTNFTNLADLSAL
HHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHH
ALPAPMRDDGLPAGITLVAPAWHDRALAAFGLRWQQAQALPLGATGRVLPPLNSLTPSAQ
HCCCCCCCCCCCCCEEEEECCCCCHHHHHHCCCHHHCCCCCCCCCCCCCCCCHHCCCCCC
HVQVAVVGAHLSGMPLNFQLTQRAAILLEQTTTAPCYRLYALANTQPPKPGLVKTEQGAA
EEEEEEEEECCCCCCCEEEEHHHEEEEEECCCCCCCEEEEEECCCCCCCCCCEECCCCCE
IRVELWEIPLARFGELVAEIPAPLGIGTLLLADGRRVKGFICEPWALQGATDITEFGGWR
EEEEEECCCHHHHHHHHHHCCCCCCCCEEEEECCCEEEEEEECCCCCCCCCHHHHHCCHH
NYVASLKGA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: monocarboxylic acid amide; H2O

Specific reaction: A monocarboxylic acid amide + H2O = a monocarboxylate + NH3

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA