Click here to switch to the map view.

The map label for this gene is bioF

Identifier: 157369555

GI number: 157369555

Start: 1441099

End: 1442250

Strand: Direct

Name: bioF

Synonym: Spro_1312

Alternate gene names: 157369555

Gene position: 1441099-1442250 (Clockwise)

Preceding gene: 157369554

Following gene: 157369556

Centisome position: 26.45

GC content: 62.24

Gene sequence:

>1152_bases
ATGAGCTGGCAACAACGCATTGAACAAGCGCTGGAGGAGCGGCAACAGAGCGCCGCTTACCGCCGCCGTCAGGTGAATCA
GGGTGGCAATGGCCGCTATATTCAGCTTAATGGCGAGCATTACCTGAATTTCTCCGGCAATGACTACCTGGGCCTGAGCC
AGAATGCCCAGGTGATCGCCGCCTGGCAGCAGGGCGCGCAGCAGTATGGGGTCGGCAGCGGTGGCTCTGGCCACGTTACC
GGTTTTTCTACGGCACATCAGGTGCTGGAACAGCAACTGGCGGACTGGCTTGGCTACCCGCGTGCGTTGCTGTTTATCTC
TGGCTATGCCGCCAACCAGGCGCTGCTGGCGGCTCTGATGCAGGCCGAGGATCGCATTTTAGCCGACCGGCTCAGCCATG
CTTCTTTGCTGGAGGCGGCTGCCCACTCACCGGCCCAGTTGCGTCGTTTTCGTCATAATCAGCCCCAGGCGCTGGCTGAT
TTGCTTGCCAAACCCTGCGACGGTCAAATTTTGGCGGTGACCGAAGGGGTATTCAGCATGGACGGCGACAGTGCGCCACT
GGCGGAGCTGCACCGTTTGACCCGCGCGGCGGGGGCCTGGCTGATGGTCGATGACGCCCACGGTATCGGCGTGCACGGCG
AGCAGGGGCGCGGCAGTTGCTGGCAGCAGGGTGTACGGCCCGAACTGTTGGTGGTCACTTTCGGCAAAGCCTTTGGCCTC
AGCGGTGCCGCAGTGTTGTGCGATGAACCCACCGCCGAATATCTGCTGCAGTTTGCCCGCCATTTGATTTACAGCACCGC
GATGCCACCGGCACAGGCCAGCGCATTGCAGGCAGCGTTGCATTGCATTCGGCAGGGCGACGAATTACGTGCCCGGCTGC
AGGATAACATTCAGCGTTTCCGCCAGGGAGCGGCCCAGTTGTCGCTGACCCTGACGGAGTCGGTGACCGCCATTCAACCT
TTGCTGGTGGGCGATAACCAACGAGCGCTCGATCTGGCGCAAAGCCTGCGCGAGCAGGGCCTGTGGGTGAGTGCTATCCG
CCCGCCAACGGTGCCACCGGGCGGCGCGCGCTTGCGCATTACCCTGACGGCGGCACATCAGCCGCAGGATATCGATCGTC
TGCTGGAGGTATTGCATGACGTCAGCGTTTGA

Upstream 100 bases:

>100_bases
AGCAGACCGAAACCGAGCACGGCGACAACCAGCAACAGCAGGCGCTGGCTGCGCAGTTGATGCAGGCGGATACCGCTGAG
TTTTACAACGCGGCACTGTA

Downstream 100 bases:

>100_bases
ACCGATCAATAAGCAGGCGGTGGCCTCGGCCTTCAGCCGTGCCGCTGGCAGCTATGACGCGGCGGCGGTGCTGCAGCGTG
AAGTTGGTGAGCGCTTGCTG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSWQQRIEQALEERQQSAAYRRRQVNQGGNGRYIQLNGEHYLNFSGNDYLGLSQNAQVIAAWQQGAQQYGVGSGGSGHVT
GFSTAHQVLEQQLADWLGYPRALLFISGYAANQALLAALMQAEDRILADRLSHASLLEAAAHSPAQLRRFRHNQPQALAD
LLAKPCDGQILAVTEGVFSMDGDSAPLAELHRLTRAAGAWLMVDDAHGIGVHGEQGRGSCWQQGVRPELLVVTFGKAFGL
SGAAVLCDEPTAEYLLQFARHLIYSTAMPPAQASALQAALHCIRQGDELRARLQDNIQRFRQGAAQLSLTLTESVTAIQP
LLVGDNQRALDLAQSLREQGLWVSAIRPPTVPPGGARLRITLTAAHQPQDIDRLLEVLHDVSV

Sequences:

>Translated_383_residues
MSWQQRIEQALEERQQSAAYRRRQVNQGGNGRYIQLNGEHYLNFSGNDYLGLSQNAQVIAAWQQGAQQYGVGSGGSGHVT
GFSTAHQVLEQQLADWLGYPRALLFISGYAANQALLAALMQAEDRILADRLSHASLLEAAAHSPAQLRRFRHNQPQALAD
LLAKPCDGQILAVTEGVFSMDGDSAPLAELHRLTRAAGAWLMVDDAHGIGVHGEQGRGSCWQQGVRPELLVVTFGKAFGL
SGAAVLCDEPTAEYLLQFARHLIYSTAMPPAQASALQAALHCIRQGDELRARLQDNIQRFRQGAAQLSLTLTESVTAIQP
LLVGDNQRALDLAQSLREQGLWVSAIRPPTVPPGGARLRITLTAAHQPQDIDRLLEVLHDVSV
>Mature_382_residues
SWQQRIEQALEERQQSAAYRRRQVNQGGNGRYIQLNGEHYLNFSGNDYLGLSQNAQVIAAWQQGAQQYGVGSGGSGHVTG
FSTAHQVLEQQLADWLGYPRALLFISGYAANQALLAALMQAEDRILADRLSHASLLEAAAHSPAQLRRFRHNQPQALADL
LAKPCDGQILAVTEGVFSMDGDSAPLAELHRLTRAAGAWLMVDDAHGIGVHGEQGRGSCWQQGVRPELLVVTFGKAFGLS
GAAVLCDEPTAEYLLQFARHLIYSTAMPPAQASALQAALHCIRQGDELRARLQDNIQRFRQGAAQLSLTLTESVTAIQPL
LVGDNQRALDLAQSLREQGLWVSAIRPPTVPPGGARLRITLTAAHQPQDIDRLLEVLHDVSV

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily

Homologues:

Organism=Homo sapiens, GI7657118, Length=384, Percent_Identity=32.5520833333333, Blast_Score=194, Evalue=8e-50,
Organism=Homo sapiens, GI284448556, Length=347, Percent_Identity=34.0057636887608, Blast_Score=189, Evalue=3e-48,
Organism=Homo sapiens, GI83977442, Length=403, Percent_Identity=32.0099255583127, Blast_Score=174, Evalue=1e-43,
Organism=Homo sapiens, GI83977440, Length=403, Percent_Identity=32.0099255583127, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI83977444, Length=403, Percent_Identity=32.0099255583127, Blast_Score=173, Evalue=2e-43,
Organism=Homo sapiens, GI4502025, Length=399, Percent_Identity=31.5789473684211, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI40316939, Length=399, Percent_Identity=31.5789473684211, Blast_Score=163, Evalue=2e-40,
Organism=Homo sapiens, GI119220554, Length=361, Percent_Identity=28.808864265928, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI4758668, Length=365, Percent_Identity=27.6712328767123, Blast_Score=141, Evalue=9e-34,
Organism=Homo sapiens, GI5454084, Length=201, Percent_Identity=27.363184079602, Blast_Score=86, Evalue=5e-17,
Organism=Escherichia coli, GI1786993, Length=381, Percent_Identity=67.1916010498688, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI1790046, Length=349, Percent_Identity=35.8166189111748, Blast_Score=209, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI71994529, Length=385, Percent_Identity=30.6493506493506, Blast_Score=205, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI32566772, Length=363, Percent_Identity=25.6198347107438, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17560914, Length=360, Percent_Identity=25.2777777777778, Blast_Score=118, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17560912, Length=360, Percent_Identity=25.2777777777778, Blast_Score=118, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI71982617, Length=306, Percent_Identity=22.8758169934641, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI71982625, Length=306, Percent_Identity=22.8758169934641, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320438, Length=363, Percent_Identity=29.2011019283747, Blast_Score=146, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6320267, Length=372, Percent_Identity=28.7634408602151, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323954, Length=267, Percent_Identity=26.2172284644195, Blast_Score=87, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24662918, Length=352, Percent_Identity=32.6704545454545, Blast_Score=200, Evalue=1e-51,
Organism=Drosophila melanogaster, GI17137420, Length=401, Percent_Identity=27.6807980049875, Blast_Score=141, Evalue=8e-34,
Organism=Drosophila melanogaster, GI17136286, Length=363, Percent_Identity=25.3443526170799, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24653280, Length=390, Percent_Identity=23.0769230769231, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653276, Length=390, Percent_Identity=23.0769230769231, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24653278, Length=390, Percent_Identity=23.0769230769231, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOF_SERP5 (A8GBC6)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477544.1
- ProteinModelPortal:   A8GBC6
- STRING:   A8GBC6
- GeneID:   5605373
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_1312
- eggNOG:   COG0156
- HOGENOM:   HBG649839
- OMA:   KDYASND
- ProtClustDB:   PRK05958
- BioCyc:   SPRO399741:SPRO_1312-MONOMER
- HAMAP:   MF_01693
- InterPro:   IPR022834
- InterPro:   IPR001917
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- TIGRFAMs:   TIGR00858

Pfam domain/function: PF00155 Aminotran_1_2; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.3.1.47

Molecular weight: Translated: 41675; Mature: 41543

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00599 AA_TRANSFER_CLASS_2

Important sites: BINDING 21-21 BINDING 133-133 BINDING 179-179 BINDING 350-350

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWQQRIEQALEERQQSAAYRRRQVNQGGNGRYIQLNGEHYLNFSGNDYLGLSQNAQVIA
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCEECCCCCCHHHH
AWQQGAQQYGVGSGGSGHVTGFSTAHQVLEQQLADWLGYPRALLFISGYAANQALLAALM
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEHEEECCHHHHHHHHHHH
QAEDRILADRLSHASLLEAAAHSPAQLRRFRHNQPQALADLLAKPCDGQILAVTEGVFSM
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCHHCC
DGDSAPLAELHRLTRAAGAWLMVDDAHGIGVHGEQGRGSCWQQGVRPELLVVTFGKAFGL
CCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCEEEEEEECHHHCC
SGAAVLCDEPTAEYLLQFARHLIYSTAMPPAQASALQAALHCIRQGDELRARLQDNIQRF
CCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RQGAAQLSLTLTESVTAIQPLLVGDNQRALDLAQSLREQGLWVSAIRPPTVPPGGARLRI
HCCHHHEEEEHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEE
TLTAAHQPQDIDRLLEVLHDVSV
EEEECCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SWQQRIEQALEERQQSAAYRRRQVNQGGNGRYIQLNGEHYLNFSGNDYLGLSQNAQVIA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCEEEEECCCCEECCCCCCHHHH
AWQQGAQQYGVGSGGSGHVTGFSTAHQVLEQQLADWLGYPRALLFISGYAANQALLAALM
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEHEEECCHHHHHHHHHHH
QAEDRILADRLSHASLLEAAAHSPAQLRRFRHNQPQALADLLAKPCDGQILAVTEGVFSM
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHCCCCCCEEEEEECCHHCC
DGDSAPLAELHRLTRAAGAWLMVDDAHGIGVHGEQGRGSCWQQGVRPELLVVTFGKAFGL
CCCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCHHHHCCCCCEEEEEEECHHHCC
SGAAVLCDEPTAEYLLQFARHLIYSTAMPPAQASALQAALHCIRQGDELRARLQDNIQRF
CCCEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
RQGAAQLSLTLTESVTAIQPLLVGDNQRALDLAQSLREQGLWVSAIRPPTVPPGGARLRI
HCCHHHEEEEHHHHHHHHHHEEECCCHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEEE
TLTAAHQPQDIDRLLEVLHDVSV
EEEECCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA