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The map label for this gene is bioB [H]

Identifier: 157369554

GI number: 157369554

Start: 1440062

End: 1441099

Strand: Direct

Name: bioB [H]

Synonym: Spro_1311

Alternate gene names: 157369554

Gene position: 1440062-1441099 (Clockwise)

Preceding gene: 157369552

Following gene: 157369555

Centisome position: 26.43

GC content: 58.77

Gene sequence:

>1038_bases
ATGGCCGATCGCATTCACTGGACAGTGGGTCTTGCCCAGACCCTGTTTGATAAACCTTTGCTCGAATTGTTATTTGAAGC
CCAGACGGTTCACCGTCAGCACTTTGACCCGCGTCAGGTACAGGTGAGCACGCTGTTATCAATCAAGACCGGCGCATGCC
CGGAAGACTGTAAGTACTGCCCGCAAAGCTCGCGCTATAAAACCGGCCTGGAGTCGGAGCGTCTGATGCAGGTGGAGCAG
GTGCTGGAGTCGGCGCGTAAGGCCAAGGCGAATGGATCGACCCGTTTCTGCATGGGGGCGGCGTGGAAAAACCCGCACGA
GCGCGATATGCCTTACCTGCAGCAGATGGTGCAAGGGGTGAAGGCGATGGGCATGGAAACCTGCATGACGCTGGGCACGC
TGGATGGTACGCAGGCGGAACGGCTGGCGGATGCCGGGCTGGATTACTACAACCATAACCTGGACACCTCGCCGGAATTC
TACGGCAATATCATCACCACTCGCAGCTATCAGGAACGTCTGGATACCCTGGGTAAAGTGCGCGGCGCGGGCATTAAAGT
CTGTTCCGGCGGCATTGTCGGGCTGGGTGAAACGGTGCGTGACCGCGCCGGGCTGCTGGTGCAGTTGGCCAATCTGCCGA
CGCCGCCGGAGAGCGTGCCGATCAACATGCTGGTGAAGGTCAAAGGCACGCCGCTGGCCGATAACGAAGACGTCGATCCG
TTTGATTTCATCCGCACCATTGCGGTGGCACGCATCATGATGCCGGCTTCTTACGTGCGCCTGTCCGCCGGCCGTGAGCA
GATGAACGAGCAAACTCAGGCGATGTGCTTTATGGCCGGTGCCAACTCGATCTTCTATGGCTGCAAACTGCTGACTACGC
CAAACCCGGAAGAAGACAAAGACCTGCAGTTGTTCCGCAAACTGGGCCTGAACCCGCAGCAGACCGAAACCGAGCACGGC
GACAACCAGCAACAGCAGGCGCTGGCTGCGCAGTTGATGCAGGCGGATACCGCTGAGTTTTACAACGCGGCACTGTAA

Upstream 100 bases:

>100_bases
GTGATAGACATGCTTGTAAACCAAATCATATTCGTAATGGTTGACAGTATATCCACAATATCTAAACTGACGACACTTTT
TTCATTTTGGAGACGCCGTG

Downstream 100 bases:

>100_bases
TGAGCTGGCAACAACGCATTGAACAAGCGCTGGAGGAGCGGCAACAGAGCGCCGCTTACCGCCGCCGTCAGGTGAATCAG
GGTGGCAATGGCCGCTATAT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MADRIHWTVGLAQTLFDKPLLELLFEAQTVHRQHFDPRQVQVSTLLSIKTGACPEDCKYCPQSSRYKTGLESERLMQVEQ
VLESARKAKANGSTRFCMGAAWKNPHERDMPYLQQMVQGVKAMGMETCMTLGTLDGTQAERLADAGLDYYNHNLDTSPEF
YGNIITTRSYQERLDTLGKVRGAGIKVCSGGIVGLGETVRDRAGLLVQLANLPTPPESVPINMLVKVKGTPLADNEDVDP
FDFIRTIAVARIMMPASYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTETEHG
DNQQQQALAAQLMQADTAEFYNAAL

Sequences:

>Translated_345_residues
MADRIHWTVGLAQTLFDKPLLELLFEAQTVHRQHFDPRQVQVSTLLSIKTGACPEDCKYCPQSSRYKTGLESERLMQVEQ
VLESARKAKANGSTRFCMGAAWKNPHERDMPYLQQMVQGVKAMGMETCMTLGTLDGTQAERLADAGLDYYNHNLDTSPEF
YGNIITTRSYQERLDTLGKVRGAGIKVCSGGIVGLGETVRDRAGLLVQLANLPTPPESVPINMLVKVKGTPLADNEDVDP
FDFIRTIAVARIMMPASYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTETEHG
DNQQQQALAAQLMQADTAEFYNAAL
>Mature_344_residues
ADRIHWTVGLAQTLFDKPLLELLFEAQTVHRQHFDPRQVQVSTLLSIKTGACPEDCKYCPQSSRYKTGLESERLMQVEQV
LESARKAKANGSTRFCMGAAWKNPHERDMPYLQQMVQGVKAMGMETCMTLGTLDGTQAERLADAGLDYYNHNLDTSPEFY
GNIITTRSYQERLDTLGKVRGAGIKVCSGGIVGLGETVRDRAGLLVQLANLPTPPESVPINMLVKVKGTPLADNEDVDPF
DFIRTIAVARIMMPASYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTETEHGD
NQQQQALAAQLMQADTAEFYNAAL

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=344, Percent_Identity=87.2093023255814, Blast_Score=631, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6321725, Length=317, Percent_Identity=50.788643533123, Blast_Score=326, Evalue=4e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 38333; Mature: 38202

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADRIHWTVGLAQTLFDKPLLELLFEAQTVHRQHFDPRQVQVSTLLSIKTGACPEDCKYC
CCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHCC
PQSSRYKTGLESERLMQVEQVLESARKAKANGSTRFCMGAAWKNPHERDMPYLQQMVQGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
KAMGMETCMTLGTLDGTQAERLADAGLDYYNHNLDTSPEFYGNIITTRSYQERLDTLGKV
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCCCCHHHHCCHHCCCHHHHHHHHHHHH
RGAGIKVCSGGIVGLGETVRDRAGLLVQLANLPTPPESVPINMLVKVKGTPLADNEDVDP
CCCCEEEECCCCCCCCHHHHHHHHHEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCH
FDFIRTIAVARIMMPASYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPEEDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECEEEECCCCCCCCH
DLQLFRKLGLNPQQTETEHGDNQQQQALAAQLMQADTAEFYNAAL
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ADRIHWTVGLAQTLFDKPLLELLFEAQTVHRQHFDPRQVQVSTLLSIKTGACPEDCKYC
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHCC
PQSSRYKTGLESERLMQVEQVLESARKAKANGSTRFCMGAAWKNPHERDMPYLQQMVQGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHH
KAMGMETCMTLGTLDGTQAERLADAGLDYYNHNLDTSPEFYGNIITTRSYQERLDTLGKV
HHHHHHHHHHHCCCCCHHHHHHHHHCCHHHCCCCCCCHHHHCCHHCCCHHHHHHHHHHHH
RGAGIKVCSGGIVGLGETVRDRAGLLVQLANLPTPPESVPINMLVKVKGTPLADNEDVDP
CCCCEEEECCCCCCCCHHHHHHHHHEEEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCH
FDFIRTIAVARIMMPASYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPEEDK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECEEEECCCCCCCCH
DLQLFRKLGLNPQQTETEHGDNQQQQALAAQLMQADTAEFYNAAL
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8250549 [H]