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The map label for this gene is proA [H]

Identifier: 157369213

GI number: 157369213

Start: 1065941

End: 1067194

Strand: Direct

Name: proA [H]

Synonym: Spro_0968

Alternate gene names: 157369213

Gene position: 1065941-1067194 (Clockwise)

Preceding gene: 157369212

Following gene: 157369219

Centisome position: 19.56

GC content: 60.93

Gene sequence:

>1254_bases
ATGCTCGAGCAGATGGGGAAGGCCGCCAAGCAGGCCTCCTGGCAACTGGCGGTGCTGAGCACGGCGAAGAAAAACCAGGT
GCTGTCGGTGATTGCCGACAAACTGGAAGCCGAAAGCGAAGTGATTTTACAGGCTAACGAACAGGACATGGAGCAGGCAC
GCGCCAGCGGAATGAGCGAAGCGCTGCTCGATCGCCTGTTACTGACCCCGGCACGTCTGGCGGCGATCGCCAACGACGTG
CGTCAGGTATGCCGCCTGAACGACCCGGTCGGCCACGTATTGGACGGCAGCCTGCTGGACAGCGGGTTGAAGCTGGAACG
TCGCCGGGTACCGCTCGGGGTGATTGGCGTGATTTATGAGGCGCGGCCAAACGTCACCATCGACGTCGCCAGCCTGTGCC
TGAAAACCGGCAACGCGGTGATCCTGCGTGGCGGTAAAGAAACCCACCACACCAATCAGGCGACGGTGAAGGTGATCCAG
CAGGCGCTGGAACAGTGCGGCCTGCCGGCTGCAGCGGTACAGGCGATTGAAAGCCCGGATCGCGCACTGGTGAATGAATT
GCTGCGCCTGGATCGCTACGTCGACATGCTGATCCCACGCGGCGGTGCTGGTCTGCATAAATTGTGCCGCGAACAGTCAA
CCATCCCGGTGATCACCGGCGGTATCGGCGTTTGCCACACCTATGTTGATGACAGCGTTGATTTCGACAAAGCACTGACG
GTGATCGAGAACGCCAAAATTCAACGCCCAAGCGCCTGTAACTCGCTGGAGACGCTGCTGGTGAACCGCAATATCGCTGC
AGAGTTTCTGCCTGCCTTGAGCAGCAAAATGGCGGTGGTCGGCGTTACCCTGCACGCGGCGGAGAACGCCATGCCGATGT
TGCAAGACGGCCCGGCAACGGTGGTGGCGGTCGCGGCGGAAGATTACGACGACGAATGGCTGTCGTTGGACTTGAACGTG
GCGCTGGTGGATGACATCGATCAGGCCATCGACCATATTCGCACCCATGGCACCAGCCACTCCGACGCGATCCTCACCCG
TTCACTGAGCAGCGCCGAGCACTTCGTGCGTGCGGTGGATTCAAGCGCGGTGTACGTCAACGCCAGCACCCGCTTCACCG
ACGGCGGCCAGTTCGGCCTTGGCGCAGAAGTGGCGGTCAGCACCCAGAAACTGCACGCCCGTGGCCCAATGGGCCTGGAC
GCTCTGACCACCTATAAGTGGATCGGCTACGGTGATGATTTGGTTCGCAGTTAA

Upstream 100 bases:

>100_bases
TGCCGGTCACCACTCGCAGGAAATCAGCGAAATTCTCGGTTATGAATACGGCCCGGTGGCAGTTCACCGCGACGATATGA
TTGTCAGCTAAGGAGTGAGC

Downstream 100 bases:

>100_bases
GTTTTAGTACGACGTTGAGATTTACTTTAGGGGCGCAGCATGCTGCGCCCTTTTGTATTCTGGTTCATACCGGAATTGGC
ATCGTTGCGGGCCTCAAAGA

Product: gamma-glutamyl phosphate reductase

Products: NA

Alternate protein names: GPR; Glutamate-5-semialdehyde dehydrogenase; Glutamyl-gamma-semialdehyde dehydrogenase; GSA dehydrogenase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLEQMGKAAKQASWQLAVLSTAKKNQVLSVIADKLEAESEVILQANEQDMEQARASGMSEALLDRLLLTPARLAAIANDV
RQVCRLNDPVGHVLDGSLLDSGLKLERRRVPLGVIGVIYEARPNVTIDVASLCLKTGNAVILRGGKETHHTNQATVKVIQ
QALEQCGLPAAAVQAIESPDRALVNELLRLDRYVDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHTYVDDSVDFDKALT
VIENAKIQRPSACNSLETLLVNRNIAAEFLPALSSKMAVVGVTLHAAENAMPMLQDGPATVVAVAAEDYDDEWLSLDLNV
ALVDDIDQAIDHIRTHGTSHSDAILTRSLSSAEHFVRAVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLD
ALTTYKWIGYGDDLVRS

Sequences:

>Translated_417_residues
MLEQMGKAAKQASWQLAVLSTAKKNQVLSVIADKLEAESEVILQANEQDMEQARASGMSEALLDRLLLTPARLAAIANDV
RQVCRLNDPVGHVLDGSLLDSGLKLERRRVPLGVIGVIYEARPNVTIDVASLCLKTGNAVILRGGKETHHTNQATVKVIQ
QALEQCGLPAAAVQAIESPDRALVNELLRLDRYVDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHTYVDDSVDFDKALT
VIENAKIQRPSACNSLETLLVNRNIAAEFLPALSSKMAVVGVTLHAAENAMPMLQDGPATVVAVAAEDYDDEWLSLDLNV
ALVDDIDQAIDHIRTHGTSHSDAILTRSLSSAEHFVRAVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLD
ALTTYKWIGYGDDLVRS
>Mature_417_residues
MLEQMGKAAKQASWQLAVLSTAKKNQVLSVIADKLEAESEVILQANEQDMEQARASGMSEALLDRLLLTPARLAAIANDV
RQVCRLNDPVGHVLDGSLLDSGLKLERRRVPLGVIGVIYEARPNVTIDVASLCLKTGNAVILRGGKETHHTNQATVKVIQ
QALEQCGLPAAAVQAIESPDRALVNELLRLDRYVDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHTYVDDSVDFDKALT
VIENAKIQRPSACNSLETLLVNRNIAAEFLPALSSKMAVVGVTLHAAENAMPMLQDGPATVVAVAAEDYDDEWLSLDLNV
ALVDDIDQAIDHIRTHGTSHSDAILTRSLSSAEHFVRAVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLD
ALTTYKWIGYGDDLVRS

Specific function: Catalyzes the NADPH dependent reduction of L-gamma- glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate [H]

COG id: COG0014

COG function: function code E; Gamma-glutamyl phosphate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gamma-glutamyl phosphate reductase family [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=419, Percent_Identity=38.1861575178998, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI21361368, Length=419, Percent_Identity=38.1861575178998, Blast_Score=277, Evalue=1e-74,
Organism=Escherichia coli, GI1786438, Length=417, Percent_Identity=76.0191846522782, Blast_Score=642, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17569967, Length=415, Percent_Identity=36.6265060240964, Blast_Score=274, Evalue=7e-74,
Organism=Caenorhabditis elegans, GI17569969, Length=415, Percent_Identity=36.6265060240964, Blast_Score=274, Evalue=7e-74,
Organism=Saccharomyces cerevisiae, GI6324899, Length=432, Percent_Identity=37.962962962963, Blast_Score=293, Evalue=5e-80,
Organism=Drosophila melanogaster, GI21357643, Length=415, Percent_Identity=36.6265060240964, Blast_Score=278, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016161
- InterPro:   IPR016163
- InterPro:   IPR016162
- InterPro:   IPR015590
- InterPro:   IPR000965
- InterPro:   IPR020593
- InterPro:   IPR012134 [H]

Pfam domain/function: PF00171 Aldedh [H]

EC number: =1.2.1.41 [H]

Molecular weight: Translated: 44778; Mature: 44778

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS01223 PROA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEQMGKAAKQASWQLAVLSTAKKNQVLSVIADKLEAESEVILQANEQDMEQARASGMSE
CCHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCHHH
ALLDRLLLTPARLAAIANDVRQVCRLNDPVGHVLDGSLLDSGLKLERRRVPLGVIGVIYE
HHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHCCCHHHHHCCCEEEEEEEEE
ARPNVTIDVASLCLKTGNAVILRGGKETHHTNQATVKVIQQALEQCGLPAAAVQAIESPD
CCCCCEEHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCC
RALVNELLRLDRYVDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHTYVDDSVDFDKALT
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEECCHHHHHHHCCCCCCHHHHHH
VIENAKIQRPSACNSLETLLVNRNIAAEFLPALSSKMAVVGVTLHAAENAMPMLQDGPAT
HHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCE
VVAVAAEDYDDEWLSLDLNVALVDDIDQAIDHIRTHGTSHSDAILTRSLSSAEHFVRAVD
EEEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
SSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLDALTTYKWIGYGDDLVRS
CCEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCCHHHHHEEECCCCHHHHCC
>Mature Secondary Structure
MLEQMGKAAKQASWQLAVLSTAKKNQVLSVIADKLEAESEVILQANEQDMEQARASGMSE
CCHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHCCHHH
ALLDRLLLTPARLAAIANDVRQVCRLNDPVGHVLDGSLLDSGLKLERRRVPLGVIGVIYE
HHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHCCCHHHHHCCCEEEEEEEEE
ARPNVTIDVASLCLKTGNAVILRGGKETHHTNQATVKVIQQALEQCGLPAAAVQAIESPD
CCCCCEEHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCC
RALVNELLRLDRYVDMLIPRGGAGLHKLCREQSTIPVITGGIGVCHTYVDDSVDFDKALT
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEECCHHHHHHHCCCCCCHHHHHH
VIENAKIQRPSACNSLETLLVNRNIAAEFLPALSSKMAVVGVTLHAAENAMPMLQDGPAT
HHHCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCE
VVAVAAEDYDDEWLSLDLNVALVDDIDQAIDHIRTHGTSHSDAILTRSLSSAEHFVRAVD
EEEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
SSAVYVNASTRFTDGGQFGLGAEVAVSTQKLHARGPMGLDALTTYKWIGYGDDLVRS
CCEEEEECCCEECCCCCCCCCCCEEEEHHHHHCCCCCCCHHHHHEEECCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1851803 [H]