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The map label for this gene is proB [H]

Identifier: 157369212

GI number: 157369212

Start: 1064828

End: 1065931

Strand: Direct

Name: proB [H]

Synonym: Spro_0967

Alternate gene names: 157369212

Gene position: 1064828-1065931 (Clockwise)

Preceding gene: 157369211

Following gene: 157369213

Centisome position: 19.54

GC content: 62.23

Gene sequence:

>1104_bases
ATGAACGGCAGCCAGACACTGGTTGTGAAATTGGGCACCAGCGTGTTGACCGGCGGATCGCTGCGTCTTAACCGTGCCCA
TATTGTCGAACTGGTTCGCCAGTGCGCACAACAGCATGCAGCGGGTCACCGCATCGTTATCGTCACCTCCGGCGCGATTG
CCGCCGGGCGCGAACATTTGGGTTACCCCGAACTCCCCGCCACCATCGCCTCCAAGCAGTTGCTGGCGGCGGTCGGGCAG
AGCCGGTTGATCCAACTGTGGGAGCAACTGTTCTCCATTTACGGTATCCACGTCGGGCAGATGTTGCTGACGCGTGCCGA
TCTGGAAGACCGCGAACGCTTCCTCAATGCGCGTGACACCATGACGGCGTTGCTGGACAACCGTATCGTGCCGGTAATCA
ATGAGAACGACGCGGTAGCGACCGCAGAAATCAAGGTCGGCGACAACGACAACCTGTCGGCATTGGCGGCAATCCTGGCC
GGGGCCGACAAACTGTTGTTGCTGACCGATCAGCAGGGGCTGTACACCGCCGACCCACGTAACAACCCGCAGGCAGAGCT
GATCCGTGAAGTGCACGGCATCGACGACGCCTTGCGCGCCATCGCCGGCGACAGCGTTTCCGGTCTGGGCACCGGCGGCA
TGGGCACCAAGCTGCAAGCCGCCGACGTGGCCTGCCGCGCCGGTATCGACGTAATCATCGCCGCCGGCAGCAAGCCGGGC
GTGGTGGCTGACGTGATTGAAGGCAAACCGGTGGGCACCCGTTTCCACGCGCTGGAAACCCCGCTGGAAAACCGCAAACG
CTGGATCTTCGGCGCACCACCGGCCGGTGAGATCACCGTCGACGACGGTGCGGTCGACGCCATTATGGCGCGCGGCAGCT
CACTGCTGCCGAAGGGCATCCGCGAGGTCAAAGGCGATTTCTCCCGTGGTGAAGTGATCCGCATCCGCAACCTGACGGGG
CGCGATCTGGCGCACGGCGTCAGCCGTTATAACAGCGACGCGATGCGCATGATTGCCGGTCACCACTCGCAGGAAATCAG
CGAAATTCTCGGTTATGAATACGGCCCGGTGGCAGTTCACCGCGACGATATGATTGTCAGCTAA

Upstream 100 bases:

>100_bases
AGTATTCTGCTGATTTCGCGTTGTGCCTGTTGGCATAACGCGTCTCTCCTGTTACAAAGTCCCTAGTATTATTCTTCTCA
TCTTACACGGCAGAAACACT

Downstream 100 bases:

>100_bases
GGAGTGAGCATGCTCGAGCAGATGGGGAAGGCCGCCAAGCAGGCCTCCTGGCAACTGGCGGTGCTGAGCACGGCGAAGAA
AAACCAGGTGCTGTCGGTGA

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MNGSQTLVVKLGTSVLTGGSLRLNRAHIVELVRQCAQQHAAGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQ
SRLIQLWEQLFSIYGIHVGQMLLTRADLEDRERFLNARDTMTALLDNRIVPVINENDAVATAEIKVGDNDNLSALAAILA
GADKLLLLTDQQGLYTADPRNNPQAELIREVHGIDDALRAIAGDSVSGLGTGGMGTKLQAADVACRAGIDVIIAAGSKPG
VVADVIEGKPVGTRFHALETPLENRKRWIFGAPPAGEITVDDGAVDAIMARGSSLLPKGIREVKGDFSRGEVIRIRNLTG
RDLAHGVSRYNSDAMRMIAGHHSQEISEILGYEYGPVAVHRDDMIVS

Sequences:

>Translated_367_residues
MNGSQTLVVKLGTSVLTGGSLRLNRAHIVELVRQCAQQHAAGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQ
SRLIQLWEQLFSIYGIHVGQMLLTRADLEDRERFLNARDTMTALLDNRIVPVINENDAVATAEIKVGDNDNLSALAAILA
GADKLLLLTDQQGLYTADPRNNPQAELIREVHGIDDALRAIAGDSVSGLGTGGMGTKLQAADVACRAGIDVIIAAGSKPG
VVADVIEGKPVGTRFHALETPLENRKRWIFGAPPAGEITVDDGAVDAIMARGSSLLPKGIREVKGDFSRGEVIRIRNLTG
RDLAHGVSRYNSDAMRMIAGHHSQEISEILGYEYGPVAVHRDDMIVS
>Mature_367_residues
MNGSQTLVVKLGTSVLTGGSLRLNRAHIVELVRQCAQQHAAGHRIVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQ
SRLIQLWEQLFSIYGIHVGQMLLTRADLEDRERFLNARDTMTALLDNRIVPVINENDAVATAEIKVGDNDNLSALAAILA
GADKLLLLTDQQGLYTADPRNNPQAELIREVHGIDDALRAIAGDSVSGLGTGGMGTKLQAADVACRAGIDVIIAAGSKPG
VVADVIEGKPVGTRFHALETPLENRKRWIFGAPPAGEITVDDGAVDAIMARGSSLLPKGIREVKGDFSRGEVIRIRNLTG
RDLAHGVSRYNSDAMRMIAGHHSQEISEILGYEYGPVAVHRDDMIVS

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=32.6388888888889, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=32.4137931034483, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=89.3150684931507, Blast_Score=657, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17569967, Length=274, Percent_Identity=33.5766423357664, Blast_Score=127, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17569969, Length=274, Percent_Identity=33.5766423357664, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6321822, Length=419, Percent_Identity=33.890214797136, Blast_Score=218, Evalue=9e-58,
Organism=Saccharomyces cerevisiae, GI6320506, Length=417, Percent_Identity=33.8129496402878, Blast_Score=210, Evalue=2e-55,
Organism=Drosophila melanogaster, GI21357643, Length=285, Percent_Identity=35.0877192982456, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 39152; Mature: 39152

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGSQTLVVKLGTSVLTGGSLRLNRAHIVELVRQCAQQHAAGHRIVIVTSGAIAAGREHL
CCCCCEEEEEECCHHCCCCCEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCHHHC
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADLEDRERFLNARDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MTALLDNRIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQQGLYTADPR
HHHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCEECCCC
NNPQAELIREVHGIDDALRAIAGDSVSGLGTGGMGTKLQAADVACRAGIDVIIAAGSKPG
CCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHCCCEEEEECCCCCC
VVADVIEGKPVGTRFHALETPLENRKRWIFGAPPAGEITVDDGAVDAIMARGSSLLPKGI
EEEEECCCCCCCCCHHHHCCCHHCCCEEEEECCCCCCEEECCCHHHHHHHCCCCCCHHHH
REVKGDFSRGEVIRIRNLTGRDLAHGVSRYNSDAMRMIAGHHSQEISEILGYEYGPVAVH
HHHCCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHCCCCCCEEEE
RDDMIVS
CCCEECC
>Mature Secondary Structure
MNGSQTLVVKLGTSVLTGGSLRLNRAHIVELVRQCAQQHAAGHRIVIVTSGAIAAGREHL
CCCCCEEEEEECCHHCCCCCEEECHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHCHHHC
GYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQMLLTRADLEDRERFLNARDT
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
MTALLDNRIVPVINENDAVATAEIKVGDNDNLSALAAILAGADKLLLLTDQQGLYTADPR
HHHHHCCCEEEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCEECCCC
NNPQAELIREVHGIDDALRAIAGDSVSGLGTGGMGTKLQAADVACRAGIDVIIAAGSKPG
CCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHCCCEEEEECCCCCC
VVADVIEGKPVGTRFHALETPLENRKRWIFGAPPAGEITVDDGAVDAIMARGSSLLPKGI
EEEEECCCCCCCCCHHHHCCCHHCCCEEEEECCCCCCEEECCCHHHHHHHCCCCCCHHHH
REVKGDFSRGEVIRIRNLTGRDLAHGVSRYNSDAMRMIAGHHSQEISEILGYEYGPVAVH
HHHCCCCCCCCEEEEECCCCHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHCCCCCCEEEE
RDDMIVS
CCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1851803; 1316937 [H]