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The map label for this gene is dhbF [H]

Identifier: 157369219

GI number: 157369219

Start: 1072185

End: 1073531

Strand: Direct

Name: dhbF [H]

Synonym: Spro_0974

Alternate gene names: 157369219

Gene position: 1072185-1073531 (Clockwise)

Preceding gene: 157369213

Following gene: 157369220

Centisome position: 19.68

GC content: 61.17

Gene sequence:

>1347_bases
ATGTCGGGAACTGTTTTAGCTACTGAATGGCTGCCGCTGACACCGGCGCAGTTAGACTTCTGGGACGAATTTACCTTGCA
CCCCAGGCAGCCCTTTTCCACCGTTGCCCACGTCACCGAACTCCGTGGCGCGGTGGATGAGCTGGCGCTGGCTCGGGCAA
TCACGCTCACCATGGCGGAAACGCAGGCGTTTGCCTTGCGGTTTAGCGCCGGTACTGGCGATAGCCCGCCGCTGCAGCGT
TACGACCCTCAATCGGTGCCAGCCTTGCAGCAAATTGATCTGCAGGCGCAGGCCGATCCTTATCAGGCGGCGATGCGGCT
GATGCAGGCGGATGTCGAACGACCAATAGATTTACGCAGCGAACCGGTGGCGGCCGTATGGTTGATCAAACTGGAGCCGT
CGCGCCACCTCTGGTACCTGCGGACCCATCATATTGTGATCGATGGCTTCGGTATGGCGTTGGTCGAGCACCGCTGCGCC
ACGCTATACGCGCATTTCCTTGGCAACGGCGCAGTGGGGCAGGCTCTGGGCAGCTTCAGCGCTTTCCAGCATCATGAACT
GGCCTATGCCGCTTCTGAACGTTGCTCAAAGGATCGGTTTTTTTGGCAGAACTATCTGCCGCCGTCACAGACCTTGCCGA
CGGTGCGCAAAGGCGGCCGGGAGTACGGCGTGAAATGCCTGAGCACCAGCACGGCTCTGCCGGAAGAGATTTCGCTGCGG
TTGCGTGAGCTGTCGGAACGCAGCGGTATTGGCTGGCCCGATCTGCTGCTGGCACTGTCCGCCGCCTGGCTGTTCTTTAC
TTTACCGCCGTTGCCGCATGATAAAGGCGATACGCGCACCCTATGGATGCCGGTGATGAACCGCCGCAGCAGCCAGGCGA
CCAATACTCCGGCGCTGGCGGTTAATACGCTGCCGCTGTTGGTATCACTGCGCGGGGAGGAGACGCTGGGGCGTTTTCTG
TCAGACATGGCGCAGAGGCTGCGCGAGCTGCGCAGTCACGCCGGTTATCGCTTGCGGCAGATTGCTGCCGATCGCGGCGT
GACGCCGGCTTCGCGTTTGTTTATCTCTCCGTTTATCAACGTTCAGCCGTTTGATCCGGCCGGTTTCAGCGGTTGCACCA
GCACGCGGCGGGTACTGGCCGGCGGATCTGGCGACGGCGCTAACCTGACCTTCCGTGGCCGCACCGATGCCGGCGATCTG
TATCTGGATATTGATATTTACGTGCAGCAGTTCCCCACGGCTGGGGTTGCCGACTACGGCAATGCCCTGCAGGAGTTTTT
ACTGCGTGCGTTGCAGGAAGAGGCGTTGGAGACGGCGGTCGGTGAACTGGTGGCGGTGCCCGCCTGA

Upstream 100 bases:

>100_bases
ATTCATCCCGCTTTGGCCGCTATCAAACGTAATTTGCGGCGGCGGGGTTTTGGCTTGTGTTGTTTCAACATTTGTTTATG
GCTTTTCAAAGGTAGGTAAC

Downstream 100 bases:

>100_bases
TGCCGAGCAGGTAGGGGTCGAACACGTTCGACCCGATTTACCCTTGGGTGATGTGAGAGGGCGCAGCATGCTGCGCCCCT
ACGTTTAAACAGTGTTAACG

Product: condensation domain-containing protein

Products: pyrophosphate; AMP; enterobactin; pyrophosphate; L-Seryl-AMP [C]

Alternate protein names: NA

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MSGTVLATEWLPLTPAQLDFWDEFTLHPRQPFSTVAHVTELRGAVDELALARAITLTMAETQAFALRFSAGTGDSPPLQR
YDPQSVPALQQIDLQAQADPYQAAMRLMQADVERPIDLRSEPVAAVWLIKLEPSRHLWYLRTHHIVIDGFGMALVEHRCA
TLYAHFLGNGAVGQALGSFSAFQHHELAYAASERCSKDRFFWQNYLPPSQTLPTVRKGGREYGVKCLSTSTALPEEISLR
LRELSERSGIGWPDLLLALSAAWLFFTLPPLPHDKGDTRTLWMPVMNRRSSQATNTPALAVNTLPLLVSLRGEETLGRFL
SDMAQRLRELRSHAGYRLRQIAADRGVTPASRLFISPFINVQPFDPAGFSGCTSTRRVLAGGSGDGANLTFRGRTDAGDL
YLDIDIYVQQFPTAGVADYGNALQEFLLRALQEEALETAVGELVAVPA

Sequences:

>Translated_448_residues
MSGTVLATEWLPLTPAQLDFWDEFTLHPRQPFSTVAHVTELRGAVDELALARAITLTMAETQAFALRFSAGTGDSPPLQR
YDPQSVPALQQIDLQAQADPYQAAMRLMQADVERPIDLRSEPVAAVWLIKLEPSRHLWYLRTHHIVIDGFGMALVEHRCA
TLYAHFLGNGAVGQALGSFSAFQHHELAYAASERCSKDRFFWQNYLPPSQTLPTVRKGGREYGVKCLSTSTALPEEISLR
LRELSERSGIGWPDLLLALSAAWLFFTLPPLPHDKGDTRTLWMPVMNRRSSQATNTPALAVNTLPLLVSLRGEETLGRFL
SDMAQRLRELRSHAGYRLRQIAADRGVTPASRLFISPFINVQPFDPAGFSGCTSTRRVLAGGSGDGANLTFRGRTDAGDL
YLDIDIYVQQFPTAGVADYGNALQEFLLRALQEEALETAVGELVAVPA
>Mature_447_residues
SGTVLATEWLPLTPAQLDFWDEFTLHPRQPFSTVAHVTELRGAVDELALARAITLTMAETQAFALRFSAGTGDSPPLQRY
DPQSVPALQQIDLQAQADPYQAAMRLMQADVERPIDLRSEPVAAVWLIKLEPSRHLWYLRTHHIVIDGFGMALVEHRCAT
LYAHFLGNGAVGQALGSFSAFQHHELAYAASERCSKDRFFWQNYLPPSQTLPTVRKGGREYGVKCLSTSTALPEEISLRL
RELSERSGIGWPDLLLALSAAWLFFTLPPLPHDKGDTRTLWMPVMNRRSSQATNTPALAVNTLPLLVSLRGEETLGRFLS
DMAQRLRELRSHAGYRLRQIAADRGVTPASRLFISPFINVQPFDPAGFSGCTSTRRVLAGGSGDGANLTFRGRTDAGDLY
LDIDIYVQQFPTAGVADYGNALQEFLLRALQEEALETAVGELVAVPA

Specific function: Specifically adenylates threonine and glycine, and loads them onto their corresponding peptidyl carrier domains [H]

COG id: COG1020

COG function: function code Q; Non-ribosomal peptide synthetase modules and related proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 acyl carrier domain [H]

Homologues:

Organism=Escherichia coli, GI1786801, Length=365, Percent_Identity=29.041095890411, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010071
- InterPro:   IPR009081
- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR001242
- InterPro:   IPR006163
- InterPro:   IPR020806
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00501 AMP-binding; PF00668 Condensation; PF00550 PP-binding; PF00975 Thioesterase [H]

EC number: 2.7.7.- [C]

Molecular weight: Translated: 49470; Mature: 49339

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTVLATEWLPLTPAQLDFWDEFTLHPRQPFSTVAHVTELRGAVDELALARAITLTMAE
CCCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
TQAFALRFSAGTGDSPPLQRYDPQSVPALQQIDLQAQADPYQAAMRLMQADVERPIDLRS
CEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
EPVAAVWLIKLEPSRHLWYLRTHHIVIDGFGMALVEHRCATLYAHFLGNGAVGQALGSFS
CCEEEEEEEEECCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AFQHHELAYAASERCSKDRFFWQNYLPPSQTLPTVRKGGREYGVKCLSTSTALPEEISLR
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHCCHHHCCEEEECCCCCCHHHHHH
LRELSERSGIGWPDLLLALSAAWLFFTLPPLPHDKGDTRTLWMPVMNRRSSQATNTPALA
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHCCCCCCCCCCCEEE
VNTLPLLVSLRGEETLGRFLSDMAQRLRELRSHAGYRLRQIAADRGVTPASRLFISPFIN
EECEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHEECCCCC
VQPFDPAGFSGCTSTRRVLAGGSGDGANLTFRGRTDAGDLYLDIDIYVQQFPTAGVADYG
CCCCCCCCCCCCCHHHHEEECCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCHHHHH
NALQEFLLRALQEEALETAVGELVAVPA
HHHHHHHHHHHHHHHHHHHHHHHEECCC
>Mature Secondary Structure 
SGTVLATEWLPLTPAQLDFWDEFTLHPRQPFSTVAHVTELRGAVDELALARAITLTMAE
CCCEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEC
TQAFALRFSAGTGDSPPLQRYDPQSVPALQQIDLQAQADPYQAAMRLMQADVERPIDLRS
CEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCC
EPVAAVWLIKLEPSRHLWYLRTHHIVIDGFGMALVEHRCATLYAHFLGNGAVGQALGSFS
CCEEEEEEEEECCCCCEEEEEEEEEEEECHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
AFQHHELAYAASERCSKDRFFWQNYLPPSQTLPTVRKGGREYGVKCLSTSTALPEEISLR
HHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHCCHHHCCEEEECCCCCCHHHHHH
LRELSERSGIGWPDLLLALSAAWLFFTLPPLPHDKGDTRTLWMPVMNRRSSQATNTPALA
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHHCCCCCCCCCCCEEE
VNTLPLLVSLRGEETLGRFLSDMAQRLRELRSHAGYRLRQIAADRGVTPASRLFISPFIN
EECEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHEECCCCC
VQPFDPAGFSGCTSTRRVLAGGSGDGANLTFRGRTDAGDLYLDIDIYVQQFPTAGVADYG
CCCCCCCCCCCCCHHHHEEECCCCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCHHHHH
NALQEFLLRALQEEALETAVGELVAVPA
HHHHHHHHHHHHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Phosphopantetheine. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 6 ATP; L-serine; 2,3-dihydroxybenzoate [C]

Specific reaction: 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Seryl-AMP 6 ATP + 3 L-serine + 3 2,3-dihydroxybenzoate = 6 pyrophosphate + 6 AMP + enterobactin ATP + L-serine = pyrophosphate + L-Ser

General reaction: Transferases; Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11112781; 9384377; 10568751; 8550523 [H]