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The map label for this gene is rnhA

Identifier: 157369156

GI number: 157369156

Start: 1010371

End: 1010838

Strand: Reverse

Name: rnhA

Synonym: Spro_0911

Alternate gene names: 157369156

Gene position: 1010838-1010371 (Counterclockwise)

Preceding gene: 157369158

Following gene: 157369154

Centisome position: 18.55

GC content: 56.2

Gene sequence:

>468_bases
ATGCTCAAACAGGTAGAAATTTTCACCGACGGCTCCTGCCTTGGCAACCCGGGCCCCGGTGGTTATGGTGCAATACTGCG
CTATAAGCAGACGGAAAAAACCTTCAGCGCGGGCTTCCGCCTGACCACCAACAACCGCATGGAGATGATGGCGGCGATTG
TCGCGCTGGAAGCTCTGACCACGCCCTGCGAAGTGACCCTGAGCACCGACAGCCAGTATGTACGCCAGGGCATTACCACC
TGGATCCACAACTGGAAAAAGCGCGGCTGGAAAACGGCCGACAAAAAACCGGTCAAGAATGTCGATTTATGGCAGCGGTT
GGATTTGGCCATTCAACGCCATACGGTTAAATGGGAATGGGTCAAGGGCCACGCCGGCCATCCGGAAAACGAACGCTGCG
ACGTGCTGGCGCGTGATGCGGCCAGCAATCCAACCCAGGATGATGTCGGTTACAAACCGGAAAGTTAA

Upstream 100 bases:

>100_bases
CAATCTGTCTGGTGGTTGTAGAGATCATATTGCAGTGCTCGCAGCGCTTGTTTATGTCAGACTTGGCTTTTATAAAACTG
ATAGGAAGAGTCTACCAGAG

Downstream 100 bases:

>100_bases
GGCAGTTTGCGATAACTCTTGGTCGCGCCGACGGCGCGGCTGAGAGCAGGTTTTCTCGCACCGAATTTCATCGGCGTTGG
CGTCAGCGGTAAGGTGCGCT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MLKQVEIFTDGSCLGNPGPGGYGAILRYKQTEKTFSAGFRLTTNNRMEMMAAIVALEALTTPCEVTLSTDSQYVRQGITT
WIHNWKKRGWKTADKKPVKNVDLWQRLDLAIQRHTVKWEWVKGHAGHPENERCDVLARDAASNPTQDDVGYKPES

Sequences:

>Translated_155_residues
MLKQVEIFTDGSCLGNPGPGGYGAILRYKQTEKTFSAGFRLTTNNRMEMMAAIVALEALTTPCEVTLSTDSQYVRQGITT
WIHNWKKRGWKTADKKPVKNVDLWQRLDLAIQRHTVKWEWVKGHAGHPENERCDVLARDAASNPTQDDVGYKPES
>Mature_155_residues
MLKQVEIFTDGSCLGNPGPGGYGAILRYKQTEKTFSAGFRLTTNNRMEMMAAIVALEALTTPCEVTLSTDSQYVRQGITT
WIHNWKKRGWKTADKKPVKNVDLWQRLDLAIQRHTVKWEWVKGHAGHPENERCDVLARDAASNPTQDDVGYKPES

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=142, Percent_Identity=36.6197183098592, Blast_Score=79, Evalue=2e-15,
Organism=Escherichia coli, GI1786408, Length=154, Percent_Identity=83.1168831168831, Blast_Score=272, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI115532542, Length=140, Percent_Identity=35, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17505482, Length=132, Percent_Identity=37.1212121212121, Blast_Score=69, Evalue=8e-13,
Organism=Drosophila melanogaster, GI17137476, Length=145, Percent_Identity=39.3103448275862, Blast_Score=91, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45552509, Length=145, Percent_Identity=39.3103448275862, Blast_Score=90, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_SERP5 (A8GA77)

Other databases:

- EMBL:   CP000826
- RefSeq:   YP_001477145.1
- ProteinModelPortal:   A8GA77
- SMR:   A8GA77
- STRING:   A8GA77
- GeneID:   5603798
- GenomeReviews:   CP000826_GR
- KEGG:   spe:Spro_0911
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- BioCyc:   SPRO399741:SPRO_0911-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17520; Mature: 17520

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKQVEIFTDGSCLGNPGPGGYGAILRYKQTEKTFSAGFRLTTNNRMEMMAAIVALEALT
CCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHCCEEEECCCHHHHHHHHHHHHHHC
TPCEVTLSTDSQYVRQGITTWIHNWKKRGWKTADKKPVKNVDLWQRLDLAIQRHTVKWEW
CCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEE
VKGHAGHPENERCDVLARDAASNPTQDDVGYKPES
EECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKQVEIFTDGSCLGNPGPGGYGAILRYKQTEKTFSAGFRLTTNNRMEMMAAIVALEALT
CCCEEEEEECCCCCCCCCCCCCCEEEEECCHHHHHHCCEEEECCCHHHHHHHHHHHHHHC
TPCEVTLSTDSQYVRQGITTWIHNWKKRGWKTADKKPVKNVDLWQRLDLAIQRHTVKWEW
CCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEE
VKGHAGHPENERCDVLARDAASNPTQDDVGYKPES
EECCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA